Ajusted the fertilizer ratios to meet more optimal solution.

D72 - This week's diary starts late since I was gone on a short trip. The girls didn't miss me though as they are simply doing their thing, and with the automatic drip system, I don't even have to worry about watering. The girls look amazing! The colas are huge with plenty of frost. I'm very chuffed with how this grow is turning out :) D73 - Gave each girl 1.25-liters of water @ pH 6.6, with 25ml EM-1. I'm fighting against temps and humidity since we have a heatwave with thunderstorms and plenty of rain. The average temp in the tent the last 24 hours was 31 degrees C (88F) and RH 63.5%, which is way high this late in flower. D74 - Yes! Our bad weather has moved on, and the humidity in the tent has dropped significantly to the mid-low 50s. Much better. Have to avoid that budrot! There isn't much to do in the tent now when everything is automated, so I spend more time observing and scouting the girls. I figured out that using my hyper-bright flashlight below the canopy is an excellent way to spot leaves that are yellowing from lack of light. I never thought of that before, but damn, it worked great and will now be a new tool in my arsenal. I removed the yellowing leaves and a couple of other fan leaves blocking bud sites. The rest of the leaves I tucked. Speaking of fan leaves, the stalks on the first girl are purple while they are green on the second. Another difference is that the first look frostier than the second girl. Not sure if that means that the second girl will take longer. One fan leaf on the first girl has a strange discoloration in the middle of a fan leaf, but I'm not overly concerned, considering it is only one leaf, and we're towards the end of the grow. Finally, and then I'll stop harping about fan leaves; one leaf tip on the second girl is turning purple. D76 - I had a first look at the trichomes. Clear/milky on both girls, with a random amber here and there on the second girl. Based on this, I guess chop day is at least a couple of weeks away. D77 - We have reached the end of the sixth week of flower, and everything is trucking along. It's mainly a waiting game at this point, waiting for the buds to fatten up and for the trichomes to mature. I ordered nematodes a few weeks ago and forgot to use them. I don't have a fungus gnat infestation, so it was only a preventative measure anyway. The nematodes were a couple of weeks past their use before date, so no idea if they are still viable, but I figured it is better to use them than throw them. I gave each girl 1.25-liters of nematode water @ pH 6.6, adding 25 ml of EM-1.

Day 31 of flower Basically the girls are on autopilot, with the autopots and Gaia green there really isn't much to do, both methods are super user friendly and the basic needs that need to be meet are just ph water in the reservoir every couple days and then watch them stack buds on top of buds...Occasionally I move a branch over to another square in the net to make room but Basically after the last top feeding it's just watch and weight, pun intended....

To tell the truth, I am very surprised myself, how amazing she is FastBuds Gorilla cookies, she copes well with a small space:) she drinks 1 liter every day :) everything looks better than expected:) good luck to everyone.

Sponsored by: 🌸SUPER SATIVA SEEDS CLUB🌸-💡VIPARSPECTRA💡-💐GREEN BUZZ LIQUIDS💐-🛠️WEDRYER🛠️ 29/9 it was a tough month for me but I'm back in charge. We are almost at the end of the cycle and the flowers begin to be large and the pistils begin to color.The supercropping responded great, although I will definitely be paying more attention when crimping next time __________________________________________ Personal advertising (contains affiliate links) __________________________________________ 🦄Super Sativa Seeds Club, creators of premium and legendary genetics since 1985! Anonymous shipping! ✅https://bit.ly/Supersativaseeds __________________________________________ Did you know that Green Buzz Liquids fertilizers are 100% vegan? A complete line of products ready to give the best to each of your plants! Visit the site and see my journals to see how they work 🦄 🤯 And with the code "dreamit" you will immediately receive a 15% discount on your purchases ✅https: //bit.ly/GreenBuzzLiquidsPro __________________________________________ 👀 Are you looking for a good lamp to start with? 👀 🌞Viparspectra has something more than the others, take a look at their site. ⏩ Use "GDVIP" for an extra discount or "DREAMIT3" for an extra 5 %% discount 👀 Search for it on Amazon ✅Amazon USA: https://amzn.to/30xSTVq ✅Amazon Canada: https://amzn.to/38udUVe ✅Viparspectra UE: bit.ly/ViparspectraUE ✅Viparspectra USA: bit.ly/ViparspectraUS ______________________________________________ 🌈 Tired of blowing on your weed hoping it dries quickly? Check out the Wedryer website! You will find a well-made accessory that will help your weed dry in just 8-10 days without the annoying risk of finding mold or other annoyances! (no affiliate links) ✅https: //bit.ly/Wedryer_ ______________________________________________ 📷🥇Follow the best photos on Instagram 🥇📷 https://www.instagram.com/dreamit420/ Backup https://www.instagram.com/dreamit4200/ 🔻🔻Leave a comment with your opinion if you pass by here🔻🔻 🤟🦄💚 Thank you and enjoy growth 💚🦄🤟

@twenty20Mendocino R&DUpdate ~ Let’s Go day 49 for these girls an we are looking beautiful! This week we gave them another trim up a tied them down more a today they have bounced back like nothing happened, strong ones they gonna be an so nice an healthy! The Sangria is looking really beautiful, she’s Been flowering for lil over 2 weeks now so we still got a good amount of weeks for her to build a little more but she is already looking amazing, can’t wait for her to be done, smelling delicious! Hope you all enjoy an have an amazing productive day as well as a great safe weekend! Peace love positive vibes to all y’all Cheers 😶‍🌫️💨💨💨💨🤙🏻

the girl went to the tent on the 69th, they predict very bad weather, which would probably cause her to rot, I will try to finish the growth in the tent, she has 14 days left and will be cut, I am going on vacation, so I am forced to do it a little earlier... In general, the girl is very it's healthy, it barely fit in the tent, so a fair amount of leaves were stuck to the edges of the tent, I've never seen anything like it, that people carry from nature to the tent - and not from it to nature :D it will be interesting to see how it all ends :) good luck:)

21 días desde su germinación.

Day 79: Second Grow We've decided against using additional nutrients and have completely stopped fertilizing. The light cycle has been reduced to 11 hours, and we're already starting to give them cooler water. Starting Monday, I will be watering with ice water to stress the plants further, aiming for higher yield, more terpenes, and increased frost. The adjustment to the light cycle should also help the plants ripen evenly. The lights are hanging about 20 cm above the buds, which are getting thicker every day, soaking up the light intensely. In about a week, most of the plants should be ready for harvest. We plan to stop watering them for about 100 hours before harvest. During this period, I'll place ice cubes on the soil to shock the roots one last time. 48 hours before harvest, I will turn off the lights, giving the plants two days of darkness. After this period, the plants will be cut and hung to dry.

Day 56 - She grew 6cm this week. Not a lot... but she continues to grow well. Day 56 - Tent configuration changed: 4x more CREE CXB3590's have been added and 4x blurple LED things removed. Lights now consist of 8x CXB3590's & 1x 1000W HID with flower bulb.

14.10.24 BT# 24 Servus Leute ✌️ Woche 4 der Blüte ist angebrochen und die Pineapple Express riecht immer mehr nach Ananas. Ihre Buds werden immer dicker und bekommen langsam eine Struktur, die Buds sind ziemlich fest (Dicht). Die Farben erinnern auch immer mehr an eine Ananas Barney's Farm hat da ein ganz feinen Strain entwickelt mit einer sehr guten Gentik. Gestern gab es wieder Nährstoffe mit ner Extra Portion Phosphor was sie jetzt dringend braucht. Vielen Dank fürs Vorbeischauen das nächste Update kommt nächste Woche in diesem Sinne bleibt Cool & Stay High. Mögen all eure Ladys Gesund und prächtig wachsen und euch mit schmackhaften Stuff versorgen 💚😍 ■■■■■■■■■■■■■■■■■■■■■■■■■ 14.10.24 BT# 24 Hi folks ✌️ Week 4 of flowering has begun and Pineapple Express smells more and more like pineapple. Her buds are getting thicker and thicker and slowly getting a structure, the buds are quite firm (dense). The colors also remind more and more of a pineapple. Barney's Farm has developed a very fine strain with very good genetics. Yesterday she was given nutrients again with an extra portion of phosphorus, which she now urgently needs. Thanks for stopping by, the next update will be next week so stay cool & stay high. May all your ladies grow healthy and splendid and provide you with tasty stuff 💚😍

Está genética ya la cosecha muchas veces teniendo muy buenos resultados en todas sus cosechas. En el caso de este esqueje el resultado fue el correcto Aunque tuvo sus inconvenientes como la flora antes de tiempo la cual fue batalla con un sodio el cual me sirvió para poner a revegetar el mismo, el olor que presenta esta genética en la fase de floración es muy fuerte y rico. Es una de mi genética favorito la cual recomiendo para todos los cultivadores principiantes y más experimentados ya que es una planta Qué es fácil de cultivar y que tiene muy buenos resultados

left only the top four main cola leaves on each plant. lights down to 12/12 stretch has been induced. The plants have been mainlined continously.

Day 28: I got home from 10 days of holidays and these girls had cruised along as if I had been there with them the whole time. They are very happy, especially the middle one. The small one on the right has always been small, is what it is. She could be a stack little shorty. Time will tell. I did a feed today, top dressed according to Gaia Green at 15 ml/ gal of media which should last approx 2 weeks. I also added a bit more 444 just to make sure there’s enough N during this stretch phase. All 3 have pistols showing and are starting to flower. I was unable to train as much as I’d have liked but is what it is. Sometimes the more indica strains I’ve done are negatively effected by topping and excess training. I removed a couple huge fans that were shading too much of the plant. I also am trying a “super cropping” of top fans to get them out of the way but still save them as a nute source if they need. We’ll see how it goes. The Vipar spectra xs2000 is killling it as usual. As I’ve said 50 times it’s super powerful and I’ve had to raise the light above recommended height as I started to see leaf edge curl and light stress. No problem at all tho, better too powerful and raise it than too weak and have loose buds. I did a micro top just to stop excessive apical meristem stretch and leaving all the other lower branches too low for effective light absorption or burn on the top cola. Happy Growing 🌱

another week on full dose. 3-4 weeks remaining.

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

16 days from seed. Very impressed with progress thus far. CanndyMann Super Soil is my go too.

————————————————————— WEEK 4 / DAY 22-28 Mars Hydro FC-E3000 Floragard Professional GrowMix 11L Plant bag made of fleece Light: 55cm / 50%; Schedule: 24/0; PPFD: 592 umol/m2/s 20° C - 75 RH 300ml per Plant every 2-3days PH 6,5-7 1ml BioGrow; 0ml TopMax ; 0ml BioBloom 1ml CalMag #1 Royal Critical Automatic #2 Royal Critical Automatic Fan, extractor and pump ON 24/0. ————————————————————— -10.02.2025 The third vegetation week begins, the ladies look really good. The lamp has been set to 55cm and is running at 50%. The lowest leaves with the rust-colored spots have been removed. -13.02.2025 The GrowBox was cleaned and disinfected once. The lowest/oldest leaves were cut off. As the soil had settled after a few weeks, the pots were filled with fresh soil. -16.02.2025 Today is the last day of the third week of vegetation. The plants look good, so far everything is quite unproblematic. Total Time: 701:00h Total Energy: 107.07kWh