I didn't get a wet weight, but will update after it completely dry

@MephistoGenetics, Hi all the happy people here in GrowDiaries. This is my second cultivation ever and it will be fun to try a bigger space than my closet grow. First, I'm just going to say I'm done with the construction of my new growroom. The room is 2.14 meters by 1.7 meters and has a ceiling height of 2 meters. It provides a floor area of ​​3.6 square meters. I use a 54 Watt Lightwawe T5 for germination and 2 Pcs 400 Watt HPS lamps. I have a channel fan that replaces the room air about 40 times an hour to get a comfortable environment in the room, the air enters a fresh air intake from the outside. The air is purified through a carbon filter to then leave the room to the rest of the basement. Then I use that heat to heat the rest of the basement. I will use 10 pcs 15 liter Autopots to grow with and a 100 liter water tank that supplies the pots of water and nutrition. I will grow completely organically in soil and will watercure my buds to get the best possible medicine for me. But there are no cultivation rooms to be displayed here, so I continue with what is most important. I am very excited to see how the new growroom will work and how this Illuminauto 21 - Sour Crinkle will turn out. Illuminauto 21 - Sour Crinkle (Grape Crinkle x Sour Crack) Two of our fastest and frostiest varieties collide to make a sweet and sour delight! Expect super frosty, compact nugs that won't be a toke for novices. Destined to be a great strain for extraction work too! Plant size - Small - Medium Cycle time - 60-65 days from sprout Indica/Sativa - 80/20 Frost level - Extra Ridiculous https://www.mephistogenetics.com/product-page/illuminauto-21-sour-crinkle -------------------------------------------------------------------------------------------------------- 2017-10-02. New week. Added pics and video. The girl is 30 cm high and starting to bulk up, there is a little frost beginning to show. Starting to feed them more nutrients now. ---------------------------------------------------------------------------------------------------- 2017-10-06. Added some closeup of this beautiful girl.

Long time since I've been here!... finally back and growing crack!.. well weed... well u know.. Finally got back on here.. these only pics I have left from this grow.. this one been a journey thru and thru.. as u can see that both trees haha. Switched to the HLG a month or so ago and they were light burned pretty quickly.. this light pretty intense.. 50% too strong for veg even at 30 inches away.. thought it was nute burn or overwatering.. eventually solved it. Backed light to 40% and things got 100% better. So if u have this light the less the better at first! Put them in flower this week. So I'll be updating those pics next! They both doing great other then the light burn which I know figured out... next issue will be when to turn it up for flower and when to bring lights closer .. figure once I see first pistil. Signs pistol. Flower I'll turn it up to 50% but keep same distance.

Esa familia, ya hemos vuelto fumetillas, ya estoy de nuevo activo para explicaros mis métodos para cultivar vuestra yerba. Bueno contamos esta vez con una cookies gelato de royalqueenseeds, la verdad por oídas y lo que leí, que son una cepa bastante fuerte tiene bastante thc, veremos estas semanas cómo avanzan. Si germinación 100%, 5/5 ejemplares germinados y preparados para brotar. . Hasta ahora el ph lo dejamos en 5.8 la temperatura ronda los 22/24 grados y la humedad está en torno al 80%. . Ahora en la alimentación, gama agrobeta. 0,5 ml x L Piramid , vía radicular. 0,5 ml x L Growth black line , vía radicular. 0,1 gr x L Cancerbero , vía radicular. 0,1 ml x L Tucán , vía radicular. 0.1 ml x L Flash Root , vía radicular. 0,4 ml x L Great Green , vía foliar. . Empezamos con muy buen pie, espero que no surjan muchos problemas en futuras semanas, buenos humos 💨💨💨.

During my previous grow, I harvested and made some bubble hash. Afterward, I dropped the used, wet buds onto this coco, and it seems some seeds sprouted from the leftover material. I’m not even sure if they’re photoperiod or autoflower, but hey—let's roll with it! Both my pH meter and PPM meter are broken. I have no idea why they keep breaking so often. They’re ADWA meters, and while you can replace the reading elements, the replacements cost half the price of a new meter. It’s frustrating, and I really don’t want to keep buying them every time I start a new grow

Starting to get a bunch of dead leaves???

Están creciendo lindas y sanas, la estructura se va formando sin problema. Ya todas muestran señales de haber tomado de buena manera el cambio de fotoperiodo por lo que ya entramos de lleno a estimular raíces, cambio de fotoperiodo y próximamente engorde.

Viel gibt es zu dieser Genetik zu sagen. Seit 5 Jahren 10/10 Sterne 💚

This is my first grow. And as a first timer I have many question on whether anything I'm doing is going to be the right things. So I got these seeds of a guy on Facebook and when they came in the mail I already had question in the origin of the seeds but nonetheless I got some seeds of super lemons haze and girl scout cookies and had them going in soil within a week or so. I used a mix of regular soil and supersoil as my soil mix as well as some Coco husk hairs mix in lightly. I water every 2-3 days as the soil dry almost completely within 3 days

Week 9 their wasn’t any real changes. Everything is on par with week 8. Girl #1 looks like she’s starting to gain some more weight. I’m satisfied with her progression so far. Girl #2 still hasn’t shown any flowering. I will keep It short this week. Nothing really to report. I’m excited for the next coming weeks. Thank you for viewing my diary.

Ending up trans set 1 got a lil more space in GH...topped up the other also did some defoliation(everything) and LST HST( set 1&2)

Tag 52: Ich habe die Cookies Gelato erneut entlaubt um mehr Struktur zu schaffen. Die Pflanze bildet dermaßen viele Verzweigungen aus die auch noch so winzig sind, das ich denke mich für die falsche Pflanze entschieden zu haben. Jetzt ist es zu spät und ich muss mit dem Arbeiten was ich hier hab 😆 Tag 54: Die Pflanze wächst im zeitlupentempo weiter 😞

Today was Just the way I like it simple. No pest still lucky me. Noticed lower bud sites are forming today. Today was nute feed I reduced the calmag and the floragro by 4ml/gal and raised the bloom by 4ml/gal. Can’t wait to see what the yield will be.

Week 17 (19-5 to 25-5) 19-5 Temps: 19.8 to 25.3 degrees Humidity: 45% to 57% Watering: Both 1000 ml. EC: 1.6 Lamp strength set from 70% to 75% 20-5 Temps: 20.1 to 25.4 degrees Humidity: 46% to 55% Watering: Both 1000 ml. EC: 0.5 21-5 Temps: 19.9 to 24.3 degrees Humidity: 45% to 56% Watering: Both 1000 ml. EC: 1.6 22-5 Temps: 18.2 to 23.9 degrees Humidity: 43% to 52% Watering: Both 1000 ml. EC: 0.5 23-5 Temps: 18.2 to 23.9 degrees Humidity: 47% to 56% Watering: Both 1000 ml. EC: 1.6 24-5 Temps: 18.7 to 25.4 degrees Humidity: 50% to 54% Watering #2: 1000 ml. EC: 0.5 25-5 Temps: 19.4 to 25.8 degrees Humidity: 50% to 61% Watering #1: 1000 ml. EC: 0.5

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Ho eseguito una leggera defogliazione nella parte bassa attorno al primo e secondo nodo in modo da dare più potenza e priorità alla parte apicale verso la luce, lei è la più alta del box ed è veramente una signora! Grazie a questo super soil di Dogma Organics avrò un risultato estremamente eccezionale! Non vedo l’ora 😜

Require regular trimming and maintenance to keep them healthy as much as possible so the girls can thrives…. Take up a lot of water for sure. But will update on the way and picture . Other than that I’m happy and satisfied and will look into Cereal Milk from Premium Cultivar as well. On day 12 will take it down from hang drying in 3 b 3. I will grow 6 plants from here on out instead of 12 . Give room for plants to space out.

🌱 Terminando la semana 4 y las plantas se ven vigorosas 💪 Después del trasplante, las niñas se han mostrado bastante fuertes y adaptándose muy bien al cambio. Se nota que el trabajo previo dio resultado. Detecté una pequeña carencia de nitrógeno, así que en el próximo riego voy a incorporar unos mililitros extra de Bio Grow para corregirlo. Por ahora, todo marcha excelente y las plantas están respondiendo de maravilla. 🌿 Dato útil: Unos días antes del trasplante (semana 3), a las macetas de 11 litros les apliqué al menos 1 litro de agua con una dosis mínima de Root Juice. Esto fue para preparar y humedecer el sustrato, lo que facilitó mucho el trasplante y evitó la necesidad de regar en exceso esta semana. ¡Seguimos dándole con cariño y atención al cultivo! 🌞🌱

Finishing up this week, turned mostly cloudy last week. Plan is to chop her when trichomes are about 30% amber... likely this weekend. Going to stick her in the dark on Thursday and hopefully chop on Saturday. Tester buds seem great, nice strong head buzz, took a few branches off for my husband who prefers the head type high. Chopped her this morning... she hung and drying. We ended up with 7.3 wet ounces. Will update next week with dry weight. Smells a bit diesel/fuel like with sweet berry/grape type undertones. Skunky aroma started at the end of the week right before chopping her. Male plant did end up pollinating some of the bud. I have been finding a few green seeds here and there. Main tent doesn’t appear to be totally pollinated, a few seeds sporadically throughout the grow but most of the bud seems clear of seeds thankfully.

They are all showing preflower and chugging along nicely.