D15. Week three, and slowly things are starting to happen. She is stretching out and getting wider, and her root development looks good. I increased the DLI to 22 and plan to raise it further over the week. ------------------------------ D16. Transplanted, added worms, and watered with 1 liter of water @ pH 6.4, with one teaspoon of myco/kelp mixed in. ------------------------------ D18. Yesterday, a few of her leaves were drooping but have sprung back. (Maybe a tiny transplant shock or slight overwatering after the transplant...?) In any case, she is now growing along and looking healthy. I increased the DLI to 24 yesterday and 26 today. She doesn't need any more water yet, but I will continue to hand water until her roots have developed enough to drink from the reservoir. ------------------------------ D21. End of the third week, and all is well in the tent. She loves her new home, although I've spotted yellow tips on two new leaves and burnt tips on a couple of old leaves, so it seems that this super-soil might be a bit "hot." I'm being a bit unfair since I had to use a magnifier to spot the burnt tips. That's how minor the damage is. I increased DLI to 30, and still, no water was needed. ------------------------------

🤠🌱

Día 129 (07/10) Riego con 750 / 1.000 ml H2O de osmosis Empiezan a moderarse las temperaturas con 28 ºC de máxima Día 130 (08/10) Riego con 500 / 1.000 ml H2O de ósmosis Las temperaturas están sobre 25 °C Día 131 (09/10) Riego con 500 / 750 ml H2O de ósmosis Día 132 (10/10) Riego con 750 / 1,250 ml H2O de ósmosis Día 133 (11/10) Riego con 750 / 1,250 ml H2O de ósmosis Día 134 (12/10) Riego con 500 / 1.000 ml H2O de ósmosis Reviso los tricomas y estos indican que estamos más o menos a una semana de la cosecha! 50% trasparentes / 50% nublados Día 135 (13/10) Riego con 1 litro H2O pH 6,5 + Kelp Hidrolizado 0,3 g/L 💦Nutrients by Lurpe Solutions - www.lurpenaturalsolutions.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

Al día 55 realizamos el cambio fotoperiodo (12/12). @raizativa

Harvest time. I’m happy with the final results of my grow. The weed smell amazing citrus notes. Fiquei muito feliz com o resultado final do grow. O fumo tem um cheiro cítrico delicioso.

Forgotten Diary

One step at the time! My plant got a new house. I love this tent! Also it keep the smell inside. Now it’s time to guess if it’s a boy or a girl! I bet for a girl 💖

I think I'm on track. About 4.5-5 weeks left for these to fatten up. One of the clan (Cappadonna) started herming so I got rid of him real quick.

OK guys I'm back after being away for 2 weeks. I've proceeded to a final transplantation into a 9L AirPot container, right before I left for vacations. I also added a canopy-ring in order to open up the plant and start some LST without adjusting strings everyday. A friend was in charge of the grow room while I was away, and she did a pretty good job. The AK420 is healthy, she is the smallest plant in the room but her shape and color are amazing. Short inter-nodes. She has 8 main summits and all her apexes are about the same height. She will remain under vegetative stage for 1~2 weeks before flipping into 12/12 light schedule. I can't wait to see her full of buds! Thanks for stopping by 👊

Thursday this week has been good so far, I pulled each plant out yesterday and tidied everything up. I got rid of all the tiny lateral branches that didn’t make the cut and I got Rid of everything that was below about half way down the plant. Obviously the lateral branches that grew to the same height as the canopy are staying it’s just all the non starter stuff below. I annoyingly had my in take fan die on me. We’ll sort of, it just keeps tripping the fuse on my electric so I assume it’s from when I had my res flood onto the floor - I forgot it was there on the floor because it was hidden behind the res

Will post dry weights & more comments soon! Sorry for the lack of updates but Thanks for following & happy growing friends!✌️🏼🙏🏼🌱

Start flower: june 6 2023 The nutrients are 1ml/L calmag 1.3 grams/L drypart bloom Both from T.A. Ph around 6-6.4

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

So far so good :). Cantaloupe haze needed more runoff as leaves were clawing.

Blue Mammoth is 3 months old today and was harvested today. 440 grams wet (untrimmed and still on the branches). Going to try drying with the leaves on, then doing a dry trim this time. She smells sweet and fruity. I'll post the harvest info after she has dried and cured.

TOP Zustand

Hola mosquitos. 14.5 Week has pass and these girls like to grow. Spare is couple days late for a reason. I think i wait maybe week more or when ever spare is ready to be topped and top it first and others couple days later. If others are growing main tops while spare can grow those 4 branches couple days it might catch others. Never tried so dont know does it works. Lets see how it go. Purple Nutz is biggest still 💪 16.5 couldnt keep my hands and do just opposite than planned. Topped all others but not spare. I left 6 branches for each of those three so i can choose the best 4 of them. But NO. Purple nutz have skipped one shoot so it goes with 4 first. Second ones are thick and growing huge. Might be polyploids A.K.A leafy fuckers. Green is just normal. Only that has those 6 branches. Edge had 1 shitty and 1 abnormal branch at those first ones so those get cut and lives now with 4 branches. Spare still untouched. Somekind of issues on leaves. Lets see in couple days if its ready for topping. Didnt graft these to trunkenstein cause doesnt know how good it works. Took just clones and maybe next try go with 4 x clones from the best one in this grow. 18.5 topped spare and cut 2 extra branches from Green. Roots look great. Branches starting to take their places. Purple nuts is crazy. Those 2 abnormsl branches growing huge 🙏 cuttings all lookkng great. Purple Nutz branch is growing 5 nodes instead of 2. Crazy Girl. With High Hopes 🍀

Vamos familia, actualizamos la 4 semana de floración de estas Purple Og Kush . Y es que hasta aquí todo va correcto , tienen un buen color, se ven sanas, y van progresando las flores adecuadamente. La alimentación de Agrobeta la están aceptando muy bien , ya se las puede apreciar. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Las maximas de temperatura no superan los 26 grados y las mínimas no bajan 20, así que no me puedo quejar. Los niveles de humedad también son los correctos van entre 50%/65% de humedad relativa. Por supuesto el Ph lo estamos dejando alrededor de 6. Hasta aquí es todo, buenos humos 💨💨💨.

Day 35 is first Day with 12-12 ger. I get the lights at 60 cm above top of plants with 700 ppfd. About 50% and sprayt them with Neem oil. Placed ScroG net Day 36 lights at 828 PPFD Day 37 lights at 884 PPFD Day 38 lights at 890 PPFD, Distance 66cm Day 39 lights at 890 PPFD Day 40 lights at 890 PPFD One of my females is al little bit smaller. I tryd too feed her with some Alga Grow too see if she becomes more happy. I hope so.

Main crown got damn last week on plant with lil to no training done to it...leave are also a bit yellow going to straighten it out this week