Giovedì 17 ottobre switch a 12/12 Prossimamente cambieremo soluzione. Rinnoverò anche tutti i nutrienti con la fase vegetativa e loro continueranno a crescere fino a che iniziamo a vedere i prefiori e cui di ricambierò l'acqua con la soluzione e adatta, al momento tutto apposto! Venerdì 18 ottobre 2024 Svuoto la vasca, cambio soluzione. Partiamo da 75 lt acqua demineralizzata ec 235 ph 9.5 Aggiungo tutti I nutrienti indicati ottenendo ec 1318 ph 6.2

Videos were taken on day 37 of flower. The mac x peanut butter breath plants are looking great ones in the back left and ones in the back middle towards the right side. They have more orange pistils at this point than all the other plants and are packing on the frost. I gave all the plants just plain water with herculean harvest, enzymes and humic acids last feed and flushed it a little but and it seemed to respond well. The rocstar breaths are frosty too and smell gassy. Also, I just checked some of my DMs on here and theres alot that I've never responded to, I dont check them unless it's from a business. I put in a few diaries a while ago if anyone has any questions for me to ask them in the comment section or a journal that way people with the same or similar questions can see it and I dont have to answer the same thing 8 times. I'm happy to answer any questions about my grows and shit just try to ask them in the comments✌️

First off, that mf holds it's name very well. The smell on that strain is phenomenal. Much of fruity citrus and earthy notes and that's without the curing. I smoked a bit to get a taste. Smoke and taste quite harsh but that should go away with the curing. I harvested 634g wet, including a lot of stems, and ended up with 121g dried. they lost 80% of their mass and I might have over-dried them since I expected to get more tbh. However that's more than enough to get me through next harvest. The 3 plants turned out to be pretty different phenotype. All over pretty great but one could have used denser buds. They don't have the same maturity which means I got buds for all occasions. Kept the trimmings in the freezer for an hour and also harvested 0.67g of kief with a 220 micron bag. A lot of impurities but that was to be expected. I don't think the calcium problem has had a significant impact. Overall very happy with the product and the experience.

This week was a good recovery week, after this previous disaster at the beginning of the week. They took about 3 days to bounce back. I had to defoliate pretty heavily. You can't tell 4 days later that I did. I wanna avoid defoliating too often, as these poor buggers are already stressed. I think in about 3 days I will see if they stop growing in height, when they do... I will defoliate again. All for all, I'm pretty happy that they bounced back so well. I also have upped the EC a touch to 1.5 this week, and I'm only seeing the tiniest hint of tip burn. So I think they are at the max before it starts to really burn them. The rate they are drinking, its a struggle to keep the mix at 1.4.

Hi everyone 😁 Welcome back in another week update! Have to say girls are looking stunning 😍 frost it's almost everywhere. Trichomes on some buds up to 30% amber and progressing by hour. They are gaming weight on buds nicely as well. Aroma OMG I am sensing ripe sweet fruits, a bit woody and gasy. Planning to turn the lights off on Thursday at midnight and harvest by Sunday 😅 Wishing everyone a great week Stay tuned for daily updates Peace and love brothers and sisters ✌️💚 02/10 Day 71 Watered again approx. 2.5l of water and fish shit at 0.4 ml per 1 ltr. Runoff approx. 50-100ml. 04/10 Day 73 Everything going great. More amber trichomes, almost all pistils hairs are brown. These girls are almost ready. The Day it's just around the corner. 05/10 Day 74 Last watering, in the morning approx. 1.5l per pot. At midnight lights out for good. 06/10 Day 75 Uploaded 2 clips from last night. Trichomes are on point. Some buds 50-70% amber and they did gained some weight overnight. Just one more night of darkness and will harvest! 😎 07/10 Day 76 It's done!!! Harvested and trimmed. Soon will return with final update - Harvest 😁 Thank you all for such a great support, likes comments and pm's Peace and love brothers and sisters ✌️💚

Day 73, 25th of November 2020: Hi! Well they are getting nice maturing buds getting bigger and all good. I love the red colour of the Red Hot Cookies. Unfortunately, Tropicanna Poison and Red Mandarnine does not show any signs of purple or red colour. Kalini Asia neither albeit, the bonsai version gets purple. Gelato is little beauty. So yes, basically not much to say. Fertilization is still the same every second day with the ratio and mixture above stated. The lamp is on 11.15 min and off 12.45 min. Last week was 15 min longer light cycle.... So every week 15 min shorter light cycle until the 5th week. So far -45 min. It switches on at 6 am and off at 17.15 pm.

She is going to be huuuuuge! All branches and stems have risen up and are strengthening daily in order to produce big resin filled flowers! I love her progress as she climbed to the height of the purple punch and soon to stand taller! Can’t wait to have the final weeks approach! Thanks for stopping by buds and chuds 🤩😎💪🏽

Week 8 Flower week 2 Flower-day 5 26/10/2025 Finally feeling good again! Kept the same nutrient mix almost the same, have had to use some others just to get by until more rock up 🙃 Floramax Veg 1: 1000ml Floramax Rezin: 400ml Floramax Organabud: 400ml Floramax Flowering Enhancer: 400ml Cyclo Platinum Silica: 200ml ISN Cal-Mag: 400ml (still waiting on my main one to be delivered, so using what’s left of this) Green Planet Massive Bloom: 200ml Green Planet Bud Booster: 4 scoops (30ml each) Sleeps with the Fishez: 100ml — used some hydrogen peroxide today while waiting for the next batch to arrive next week I’ve been looking into a flushing additive called New Millennium Winter Frost for a nice clean finish. Pistils finally popped up yesterday on a few of the top sites! Should start seeing some solid stretch from LA punch soon, guessing around 30 to 40cm at least, who really knows right now 🤷‍♂️ I’m letting her do her thing and reach up into the net for now. If she starts getting crowded before the end of week 3, I’ll kick off some early defoliation. Also got two AC Infinity IonBeam S11 lights set up sitting about level with the top colas. I’m aiming the light to hit the middle and lower sections instead of blasting the tops directly, trying to get that nice even coverage. Still debating whether I even need to scrog this run, but a few people keep telling me to give it a go to see the difference, I guess only one way to tell 🙌

These are being feed flora nova bottle nutes

So I harvested on day 70, Pretty excited, I started with 12 seeds, 1 never popped, 2 got messed up in shell, but I hoped they would make it, they did, but they are embarrassing, I one the root system barley existed the other got so bushy and I didn't stay on top of it so it was all frost, no actual buds, pfff. THE OTHER GIRLS more than made up for it, I changed the light cycle to 10 on, 14 off for the last 4 days of light, and then the last 2 days no lights at all. Super happy so far, oh, I did not water at all the last 4 days, I thought that may help my rh during the dry, starting the dry a little on the plant, nope, my rh is high. still happy

Fire 🔥 she smoked nice. Smooth and got a sexy taste. Overall a easy grown and will try again

we are entering the second week, the little plant is growing well. The weather is perfect at the moment ,not too hot. I am continuing this week with the mycorrhizae and bacteria in addition to the bud candy. In all, I would have watered twice with these fertilizers during those first two weeks.see you next week😎

25th April day 46 4 plants stripped I will give them a few days to recover and flip to the 12/12 cycle

📋 The Lemonpaya was a good plant to grow, im gutted about the start of bud rot, it was only a tiny bit, 3 buds tightly packed together and it was were all 3 buds touched each other. So i removed more then i needed to of decent buds just being cautious. I should have chopped the tops a good week before i did because they were ready, and i may have gotten away without mold, and the lowers would have benefited with the extra light. After 2 weeks drying I put the buds into terplock bags, never used these bags before so I'm hoping everything works out. Stats for the GDG21 Truffle gas. Dry decent buds 94g Dry larfy buds 4g Lemonpaya Dry decent buds 62g Dry larfy buds 8g Trashed buds 21g Total decent buds=156g Total larf=12g Removed because of rot 21g Hash=7g Total=163g Light power=217.11 KW during the 115 days of the grow. 18hrs for 35 days=630hrs, 16hrs for 7 days=112, 12hrs for 70 days=840hrs. Total hours lights on=1582hrs Average watt per hour lights on=137w 1.18g/w 📋 Smoke report. This isn't what I expected at all the, I'm not a fan of lemon strains but this one only has a very slight lemony taste. Better then I was expecting. Take it easy. Back soon.

FBT1 did not like being transplanted at all ! i guess being overwatered stressed her out some, good thing it's early in a way, hopefully she wont be permanently slower 🙏 lol , anyway she's basically healthy now, although last time i watered her it was tuesday last week and now it's friday, medium is still wet though, so no water for her until she dries out some more. Realised i hadnt been spraying my kelp carefully enough the week before so i'm spraying the soil with amino to try to keep pH basically fine until i can water with cal mag and hopefully some co2 enriched water. I pHed down the spray with 0.1g of GHE dry pH down powder to 6.0... rain water is pH 5.5 so i'm okay i think. anyway fbt1 in mrB's so easy i didnt even have to water this week lol. check out the videos, let me know ! 🚀

Day75 etAujourd'hui cela fait 14 jour sans arrosage !!!!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Love every plant each one is growing differently stacking different even the visual difference of one strain growin two different ways combined smells of diesel, pez candy and lemons

This week went great sofar just had a little fright when i saw a gaint grasshopper completely chewed of a whole bud for no reason just bit the stem right off i removed it by hand because i dont want to use pesticides .... anyway im reveging some of the clones and keeping some to spray silver and make some seeds

First LST. She gets water once every 3 days. Nutrients every other watering