2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Week 5 of flower, looking 👍🏾

È stata una settimana molto calda e piena di bellissime giornate, il meteo è meraviglioso questo periodo. Le bellissime autofiorenti dutchfemseeds sono tutte vicinissime alla fase finale della fioritura e alcune sono già pronte per essere raccolte ma aspetteremo un altra settimana abbondante prima di raccogliere tutte le infiorescenze mature in maniera graduale. Molto colorate le mimosa Purple Kush e molto resinose, il resto delle varietà ognuna ha il suo aroma speciale ed inconfondibile,tutte molto resinose e piene di infiorescenze molto dure e cariche di resina appiccicosa. Sono felice perché queste varietà come sempre soddisfano a pieno le mie esigenze e sono spettacolari vederle fiorire nel giardino. Alcune sfiorano il metro ed ottanta centimetri di altezza con infiorescenze ovunque. Manca poco! Un abbraccio a tutti.

Switching to 12/12 so the girls have the first week of flowering behind them, so far without any major problems, they are out in the world and it is a pleasure to look at them.

30. Juni Riesending. Deutlich größer geworden als gedacht. Sie trinkt gerade bei den Temperaturen 3l + pro Tag. In Relation zur Kush hat sie vor dem Übergang zur Blütenbildung lange stagniert. Bin gespannt wie es weitergeht.

Starting to get a smell and buds are starting to stack. Did one more top dress with the power bloom 2-8-4. Leaves slightly curling so I’ve been adding just a little more nitrogen through the Buddha grow. Other than that, stems starting to sway so she’s gonna get heavy soon. Sugar leaves are smelling identical to fruit loops or fruity pebbles 😊

Deep Forest Auto is acclimated and us showing to be a vigorously growing plant already. She has made excellent progress already, and hopefully I can keep her thriving through the grow. Everything is looking good at the moment. Nothing more to report. Thank you Doctor's Choice, Medic Grow, and Gen1:11 nutrition. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

Week 13 not a bad week if I do say so my self …guna tie down this week and begin spraying silver thiosulfate on donor plants then next week I’ll throw the donor plants into flower and begin the actual breeding project …which ever specimen reverses first shall be collected and used to pollinate which ever one doesnt will be killed and removed acord

D54 (14/01/2021): First day for week 4 of bloom. I gave them tap water cause last time the PPM run off was a bit high. The PPM run off for Banana Kush today was higher then the run off for Glue Gelato. It's not the first time I see that so I think I can say that Glue Gelato uptake more nutrients compared to Banana Kush. If it become a problem I might consider doing two separate nutrients mix. - temp: 24-27C light ON ; 21-23C light OFF - water: PH6.7, 128 PPM approx. 4L per plant - run off: PH7 and 830PPM for Glue Gelato ; PH6.9 and 1220 PPM for Banana Kush - RH: 55% for light ON and OFF D55 (15/01/2021): I saw some brownish spots on small leaves for Glue Gelato. Last feed, Glue Gelato was the one with the lower PPM run off. I think the brown spots are due to calcium deficiency. Glue Gelato is an heavy feeder, I might give a good dose of nutrients next feed. Glue Gelato PH of the run off was on the high acceptable range. So it might also be a nutrient lock due to inappropriate soil PH. I need to check that closely... - temp: 24-27 C light ON ; 21-23C light OFF - no water - RH: 54% light ON ; 50-52% light OFF D56 (16/01/2021): I fed with 3/4 of the recommended dose for mid late flower. Run off for Banana Kush is still high. I will see how she react but so far she's the one who look the healthier! Maybe she doesn't mind having a ''hot'' soil. It's been 3-4 weeks since I did check the accuracy of my PH meter. I checked yesterday and I was off of PH0.4 (reading higher that it was suppose to be). I have a cheap PH pen so I need to check that often. Maybe the little brownish spot on Glue Gelato is due to incorrect PH in water or soil over the last week. - temp: 24-27C light ON ; 21-22C light OFF - water: PH6.2, 913 PPM less then a gallon per plant - run off: PH6.7 and 1200PPM for Glue Gelato ; PH6.8 and 1700 PPM for Banana Kush - RH: 53% light ON ; 51-52% light OFF D57 (17/01/2021): There's sing of nutrient burn on Banana Kush. It was expected. I consider doing a flush for this gal. - temp: 24-26C light ON ; 21-23C light OFF - no water - RH: 54% light ON ; 57% light OFF D58 (18/01/2021): I mixed 1/2 of the recommended dose of Max Minerals. I flushed the Banana Kush until the run off passed below 1000 PPM. I did not flush the Glue Gelato since there is no sing of nutrient burn. They don't smell that much. When I open the tent, there is a weak smell but when I play with the leaves or shake the stems now I can smell them properly. Glue Gelato have a strong pine-citrus-fruity pungent smell and Banana Kush have a smooth tropical-fruity-candy smell. Both smell soooo good and the buds are starting to look really appealing 😍😋 - temp: 24-26C light ON ; 21-22C light OFF - water: PH6.3, 325 PPM - run off: PH6.9 and 1010PPM for Glue Gelato ; PH6.9 and 920 PPM for Banana Kush - RH: 55% light ON ; 51-52% light OFF D59 (19/01/2021): After 12 days of not removing any leaves today I did removed approx. 20 leaves on each plant. From now on I will continue to remove the leaves that are blocking bud site from having enough light. - temp: 23-25C light ON ; 20-21 light OFF - no water - RH: 53-54% light ON ; 54-55% light OFF D60 (20/01/2021): I gave full recommended dose of Max Minerals, half of the recommended dose of Max Grow and 3/4 of the recommended dose of Max Bud. All base on the recommended dose of nutrients for mid late flower. I was aiming for a lower PPM level then last Banana Kush run off. I dropped de lamp of 1 inch height to be around 13-14 inches from canopy. - temp: 23-25C light ON ; 21-22C light OFF - water: PH6.0 , 775 PPM - run off: PH6.7 and 1200PPM for Glue Gelato ; PH6.6 and 1060 PPM for Banana Kush - RH: 51-52% light ON ; 45-49% light OFF

4ème semaine de floraison. Cette dame ce porte a merveille ☠️☠️💀 irrigation tous les 3 jours environ 6L, 12L/Semaine.

Much more leaves appear on her body compared to the last week. All the leaves look green, healthy, and much larger. My only concern is that her stem does not get any thicker or longer these days. I try to feed some nutrients with their density far smaller than what is written on the instruction paper. Now I attach a small camera on my pot and record her growth. Her leaves look full of energy from morning to evening, whereas they look losing strength at night. It could be nature, according to my cannabis-grower friend, so I try to prevent from interfering too much. It would be problematic, however, if it has a correlation with her thin and short stem. I would like your comment on this. Your support will enlighten me, as the LED does onto the plant. "Light, my light, the world-filling light, the eye-kissing light, heart-sweetening light!" In the city of New Leningrad, 2021-02-01. I append an mp4 video to the diary. 2021-02-02.

Day 49 flowering: Hi all , I hope your all well and healthy. I think I have made a few miscalculation errors of flowering weeks here.lol I began timing their flowering period a little later than had been flowering I think. Seeing how advanced the #2 has been the entire grow followed very closely by the #1 , and adding the natural fading and leaves dying off that is very obvious too , these are very near their ripen time. looking back over the diary to when I began timing , the buds are fairly well in process so must have begun earlier but I missed the switch with the other seedling dramas I had going on at the time. oops. I am using the time line to keep the other Gorilla girl (#4) and the switch cheese at least on track timing wise ( sort of .lol) I expect the #2 girl will be ready within 2 weeks now but will show as an early harvest on the days. I have added the Shogun Dragon Force into their feed now for a week ( 2 more feeds at least) and then its a mild feeding of the Megacrop till finish. I do love their products and have been impressed by the dragon forces result on trichs. These Darker Gorilla (#2 +#4) girls are so trich laden already and very tacky to touch with a beautiful fruity smell pushing out too. The pictures just don't compare to the live and direct context with these buds but I tried to catch their best anyway. I haven't seen any pictures on sweets seeds grows of gorilla girls that are this dark purple at all and their examples are more akin to the #1 and #3 i have . I live them appearance of the dark phenos and a sure fire selling point for them ?. @sweetseeds ?. you seen these beautiful girls guys ?. With that said the younger ladies are also doing brilliantly with the #4 starting to swell and frost up similar to her older sister#2. Her structure was set slightly looser than the other 3 to see how it altered their bud production compared. She has wider node gaps that have their own clusters with them and she has grown taller from less ties obviously but , it also created a more airy frame for the density of the buds which is a bonus in a smaller environment. I don't feel as worried about bud rot with her as I do the other 3. I plan to brutal defol one of the next autos being run to see how this affects the final result. using the same strains should be a fair comparison. The Sweet cheese has also allowed me to satisfy my curiosity and try a natural untouched grow. She has some beautiful coloured on her tight single stemmed main and the side branching is attempting to keep up with the main but nowhere near as productive . while she is a capable height , i prefer the L.S.T for shape and working on so far. Being so hands off is a new one for me too.lol Her diesel smell is really evident now and even the lowers are getting a nice chalky appearance of trichs hiding the pink hues she has going on all over. Inhave enjoyed this grow with the sweet girls and only wish I hadn't lost so many of the other strains so early on. A breeder to re visit without doubt. enjoy the pics and video folks . keep well and loved ..

Привет друзья. Моей растихе сегодня 71 дней. Был в отъезеде, не поливал. В связи этим растение не много подсохло. Как придёт в чувства. переведу на цвет. Начал применять LST технику на 19 дне и продалжаю применять её через день, а 18 августа добавил ДЕФОЛИЗАЦИЮ С 20.08 ДЕФОЛИЗАЦИЮ делаю каждые 3,4 дня С 20.08 LST технику делаю каждые 4.6 дней На сегодняшний день влажность 54% 26 дней назад заметил высокий Ph 7.9 С 48 дня Ph не ниже 5.8 На сегодняшний день Ph 6.0 Начал кормить с 60 дня Canna Terra Vega PPM 550 Погладитель влажности, выполняет свои функции. Посмотрю на динамику. Всем мира и добра! https://t.me/smail_seeds #Smail_Seeds

First week flower underway. Added liquid Koolbloom. Way down on the base nutes, this and my other girls have been showing signs of a few things. 1.Nitrogen toxicity. It was commented on my last grow question that the leaves on my Gorilla Glue grow looked like they were heavy in nitrogen. I did the research and it looks like they were correct. Dry crispy leaves, heavy dark green. They and now myself blame the full strength Advanced Nutrients, noting they are high in Nitrogen. 2. pH issues. I also started noticing a lockout isssues, like red stems. I initially thought it was a strain specific trait, but again was recommended to test my runoff - it came out around 4.5🤮. Ive been watering heavy at 6.7 now with lime on top. Hopefully will help. Also, new Meizhi 450 on the way! Really impressed with my other one and the size accommodated my space very well. My flower room is like a L shape

Bueno resumiendo cookies gelato es una variedad híbrida muy fácil de cultivar pero ojo, cuidado con los últimos riegos si no queréis perder cosecha por moho. Lo demás de 10 pegada heavy sabor increíble, cogollos duros como rocas y bañados de una gran capa de polen. El ambiente del secado se mantuvo en 23 grados de media y la humedad estuvo por debajo de los 45% en todo momento. Poco más la verdad estuve encantado de poder cultivar una genética tan potente . Un saludazo que paséis un final de año increíble y por supuesto buenos humazooos💨💨💨.

She’s onto flower. Showing Pistols 48 hours after the flip to an 11 on 13 off light schedule. She is doing great and is the biggest of the three in the tent. As she’s a true Sativa I added the first layer of trellis netting in anticipation of her stretch. 8-9 weeks of flower ahead for this girl.

I THINK THERE IS PRE-FLOWER PERIOD, WHO AGREE AND WHOS NOT ;D

Getting ready to transplant in a couple of days. Today on one of the short plants I half snapped one of the fan leaves near the top of the plant! I've also gone and knocked over another one when feeding earlier today 😔 tent should be up by now but can't put up yet ATM

Outdoors........time passes slowly.... Tropical weather here, this week. Hot morning, evening storms , cooler nights, high humidity, wind. What else? No problems to report, fantastic grow, girls full of life. Lots of work to bend and rearrange plant's shape every day, sometimes twice a day. Defoliation. Plant is responding fantastically. Special Kush #1 is now in the last days of crazy-stretch phase, is a big plant so I had to move the main top forward again to the opposite point of the perimeter in order to lower the line and give light in the middle.