I been away from grow diaries several weeks now, quick video for documentation, mute sound in video please, Andie Capp is looking so tired between 11am-1pm. As well am feeding as needed. 2-3 ounces 4-5 times a day for Yao, and 0.5 - 1 ounce 2-3 times a day for Andie.

10/10/21 new week for CBD spliff berry Growing almost a month now did some topping last week and today started to LST the main everything going great so far plants loving the new FC-4800 so as i absolutely no extra heat is coming of them 👌🏻 I maybe will top some more but as far it Goes for LST this will be the only thing i will do to the plants let the rest grow freely 14/10/21 Growing very nicely giving them the watering and nutrients when they need it . I am trying to understand plants better by looking at feeling soil etc instead of a regiment. And learning we do every day because bin growing in 11L pots but now in 8L pots so that means that there gone be smaller then my first grow . But hey got now 12 plants going on and not 4 so that will help lol 15/10/21 Did some small defoliation and some of them get light penetrating better visit www.marshydro.eu for your best gear and use the PROMO CODE: DEVILSBUD Fully marshydro supported Setup: Marshydro GT120x120x200 Marshydro FC-4800 LED lamp Mars Hydro tent handy info ●【Lock All Light Insid】MARS HYDRO Grow Tent Interior is made of diamond mylar. Non-toxic to the environment, no harm to plants. High-quality 1680D canvas being double stitched, (ALL OTHERS BRAND grow tent is 601D canvas) which is tear-proof for perfect light locking, No light leaks or rips at all. ●【Durability Grow Tent】Stable Metal corner adapters and poles are the trustful supporters of the tent. No rusting nor paint-falling. Quick easy tool-free installation. Carries strong heavy-duty SBS zippers, double layer lining to create a light-proof seal. ●【Easy Observation of your Plants】Special peeks window made by double layers of cloths. Observe the growing conditions of your plants without opening the tent and avoid disturbing plants. The peek window also serves to dissipate heat and ventilate air. ●【Removable Tray Keep Clean】The package includes a removable waterproof floor tray to hold soil and fallen leaves. The tray can be taken out easily to wash. Removable and water-proof makes cleaning easy. ●【Good Ventilation&Fast Assembly】 Circular double-sleeved vent holes with adjustable nylon strap for air circulation by exhaust fan, ducting, carbon filter, or reflector. Better allows proper light, heat, and airflow. Quick tool-free installation. Reinforced by a sturdy metal frame to ensure security and stability, supports up to 140lb. Perfectly safeguard your plants to thrive even in winter.

In the end it even turned a bit purple, probably because I had it in the living room for the last 2 weeks. It smells very different from the other Honey Cream. The smell is more fruity.... it is now standing to dry in my box after dry, theres total of 35 gramms

🌿 Woche 8 - Erste Erntewoche - So Jung und doch so Reif ️🌿 🌱 Wachstum Man sieht mittlerweile deutlich, dass die "kleinere" Pflanze es sehr eilig hat. 🏃‍♀️💨 Die Dominante Dame, lässt sich dagegen etwas mehr Zeit für ihre Buds. ⏳🌸 Aber für beide Damen gilt: Für ihre 8–9 Wochen ist das beachtlich. 🌟 Rückblickend hätte ich vllt etwas mehr Lollypopping machen sollen. Beim Ernten, kamen ein Paar Triebe zum vorschein, die sich nicht so gut entwicken konnten. Fastbuds typisch, sind viele Quallitativ geile Buds dabei. Ich freue mich schon aufs Naschen 😋 Aber jetzt wo ich die Erste Lady geerntet hab, hab ich gemerkt das 100g vllt etwas hochgestapelt war. 😬 Ich hoffe das ich wenigstens noch die 80g irgendwie knacke. 😹 🌱 Living Soil Was man auch sieht, sind Mängel. 😰 Es könnte an den 2 Pflanzen im selben Topf liegen. Grade bei so schnell wachsenden Auto´s könnte die Nährstoff Verteilung gestört gewesen sein. 😵 Es könnte aber auch sein, dass die Erde wegen der Doppelbepflanzen schon sehr ausgelaugt war. Es könnte auch sein das die Microorganismen nicht schnell genug hinterherkamen. 😪 Da ich viele Rote Stiele sowie Rostflecken an den Blättern, und etwas Leaf-rot hatte, müsste es hauptsächlich Phosphor Mangel sein. 👉 💡 Licht & Schatten PPFD 📈 Aktuell ca. 900 µmol/m²/s. ☀️ Wetter Die Temperaturen sind und bleiben brutal: 27–34 °C 🌡️🔥 💧 Gießen 🕒 Alle 2–3 Tage 🚿 Jeweils 2,2 l Wasser 💦 🎶 The Grow must go on! 🌱✨

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

There is not much to say about week 10.. I just followed my feeding plan

The ladies has been having issues since day one, but im not giving up on these fighters! I have only been feeding ph 6.3 water, with no nutrition. Monitering moisture level in soil, and feeding before dry. -Temps - 26-27 day / 22-23 night -RH% - 65%(+-1.5) day / 70% night (+-1.5) -VPD 0.8 - 1.0 KPA 👉12/9 Climate on point, still recovering. 👉13/9 Letting them be. 🙏14/9 Praying every day. 👉15/9 The 3 bigger ones, is showing signs of adapting and overcoming the struggle. 👉16/9 The 3 smaller ones is still behind by a lot. 👉17/9 Topping two of the biggest and cloning the tops. *********First nutrients added to watering. (The 3 smallest got a bit of Biobizz root juice as top feed.) 👉18/9 In the mist of gathering data, to dial in vpd. I have only been feeding ph 6.3 water, with no nutrition. Monitering moisture level in soil, and feeding before dry. -Temps - 26-27 day / 22-23 night -RH% - 65%(+-1.5) day / 70% night (+-1.5)

Very enjoyable grow, awesome cultivar, this is what I work for, my nuggets have been grown with love and dedication and the final result is a a plant that has been very thankful and she has provided me amazing sticky flower with those red hairs that I love so much, very strong cerebral buzz, sativa high definitely, makes me remember a lot sour diesel, definitely super pungent loud 🔊 aroma coming from the jars but she was stinking the grow tent when she was there, amazing strain!

Chop day today Day 80

sprayed neem two times 0.9ml/60ml and 1.7ml/70ml because invasion of the flies hasn't stopped started adding booster every other day: - 1g Booster + 4ml Mono Trace + 6ml Bio Heaven + 200 ml water - 1g Booster + 2ml CalMag + 5ml Bio Heaven + 200 ml water @PH6

I have had some troubles this week. I have lost quite a few fish (not sure why) I am also showing signs of light burn/nutrient deficiency. I had to put netting around the sides because they grew taller than they should have and started falling over from the weight. The buds are filling out quite well, some of the hairs are starting to turn orange. It wont be long before harvest.

Plants seem to be doing nicely so far I’ve not had any issues yet 🤞🏻I’m just trying to bend a top down as soon as it gets taller than the rest to try and maintain a nice even canopy ✌️🏻

Welcome back together again, brother farmers.We meet again together to test qquqques We get together again to discuss a new variety from the catalog of our friends at wse The photos speak for themselves ... it is one of the fastest varieties in this tent. He follows the feeding respecting the times and with a little training a nice bush has opened 🌱😉

Potted then up to 4ltr pots ready for flowering ✌️ switched to 12/12 on day 35

This was a good grow couple of ups and downs weather wise and the moment of devastation when seeing my fattest cola had been affected by bud rot this was a solid happy nug yesterday and today well thenpics tell a tale all other buds were fine and are drying as we speak ye win some ye lose some some are just fkin annoying

09-12-2019 I had to harvest her she was crying out and i couldn't take it no more. I also needed the room for bigger things. I have one seed left of this strain so she will return. I enjoyed my time with her but for now it's time to move to Greener Grows. Scales come in a few weeks but now expecting much as the buds were soft and loose. Thanks everyone for watching!😎

the little one is rising quickly this week, I think it will last another week, a week and a half at most, before dedicating itself to full flowering. the seedling entered the pre-flowering phase a few days ago (2/3 days ago)

Stopped using chemical nutrients and will only be using Gaia Green power bloom to top dress the soil for the remainder of the grow. This plant will most likely be a monster. Im sure it will yield a lot.

Beautiful full bodied plants that tower over the rest of the garden. Feed remains on point, humidity and heat issues in mid July weather have caused a bit of fox tails in one or two colas, but I could care less. This will be a bountiful harvest of a great strain.

Now the flowers!! 💐💐💐