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Good morning, everyone! Today, I bring mixed news from our garden. Unfortunately, the news is not the best for our beloved feminized plant. As we can see in the picture, the stem is quite thin and fragile, which compromises the healthy growth of the plant. As we know, the stem plays a fundamental role in the structure and development of the plant. It provides support for the branches and leaves, allowing them to carry out photosynthesis. Additionally, it is through the stem that water is transported from the roots to all parts of the plant. Despite having placed a support that has helped a little, only the next few weeks will tell how her development will be. I am sharing my experience with plant growth, and you are invited to follow this mystery with me. Will she reach flowering? What will be the maximum height she will reach? I will document each stage, and together we will discover the outcome of this story. On the other hand, the news is promising for our automatic plant! Since the last update, it has grown another 4 cm. The temperature this week has been quite favorable, with plenty of sun and little wind. By watering it with pH 6.5 - 7 water and using quality soil, I am confident that it will continue to thrive and reach the 5th week of vegetation in great condition. I will continue to monitor and share the progress of both plants. Until the next update! check out my instagram: @_see_weed. maybe you will like the content Loving all the processes. Can U SeeWeed?

Приветствую всех, кто следить за мной. Ваша поддержка очень важна для меня. На следующий день после того как я обновил прошлую неделю дневника наступило время пересадить растения в более крупное ведро. Я покажу вам как использовать дешовые пивные стаканы для пересадки ваших растений. Вы можете посмотреть это на первом видео. Когда я пересаживал растения я дополнительно добавил микоризу в большое ведро. Одно из растений "ЛСД -феминизированное" всё ещё показывает замедленный рост, несмотря на то, что я решил подсветить его LED полный спектр. Так же я внёс базовое питание, корневые стимуляторы и стимуляторы роста, в добавок с гуминовыми кислотами. Я планирую сделать из эти растений много клонов и запустить новый дневник в другой палатке, а эти растения потом переставить в маленькую палатку. Если вам интересно следить за приключениями травки в России, подписывайтесь на мой дневник -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Greetings to all who follow me. Your support is very important to me. The day after I updated the last week of the diary, it was time to transplant the plants into a larger bucket. I will show you how to use cheap beer glasses to transplant your plants. You can watch it on the first video. When I transplanted plants, I additionally added mycorrhiza to a large bucket. One of the "LSD-feminized" plants still shows slow growth, despite the fact that I decided to highlight its full spectrum LED. I also added basic nutrition, root stimulators and growth stimulators, in addition to humic acids. I plan to make many clones of these plants and start a new diary in another tent, and then move these plants to a small tent. If you are interested in following the adventures of weed in Russia, subscribe to my diary.

This week has been wild. I had to cut the sides of the greenhouse as the plants had it bursting at the seams. These girls are all flowering beautifully and starting to frost up. Still loving the pink pistols. Happy Growing

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

About a day into week 3 . Growing very strong and fast. Transplanted into the 5gal bags at night . Over week 3 they exploded. They wanted the room .. the 5gal bags made that happen. Amazing results with just foxfarm ocean forest soil.

Getting close! Buds are nice and thick. No amber on either but plenty of milky.

9/22 topped them at 4th node. some of them are looking better for mainlining that others. some of them i may not mainline and others i left an extra node in case i want to keep clones around. #1 is looking happy with the topping and looks really good. #2 is the only one thats been praying and is the tallest and strongest looking all around. #5 short and such tight internodal spacing that it makes it hard to see whats all there. just looks like a bunch of small leaves until they grow out. i may not mainline this one either be cause of it being so tightly packed it may make it 9/27/24 day 20 theyre growing quite well. #1 i ended up taking the bottom nodes. i was originally goi g to use them as clones later on but decided to just let her go with her 4 nodes. #5 is so tightly packed, she has like 8 mains all in such a small space, its hard to even make sense of it all.

D89 (18/02/2021): Copy paste of last week... I think max one week and she is done 😁 I gave around 2L of tap water. Glue Gelato is dry and in jar since one week. I did not move back Banana Kush in the tent since I was sure it was her last week. At this point I should have move her back in the tent. I think being in the closet might have slow her down since there is no reflective wall but at least the environment conditions are good. - temp in the closet: 18-21C - water: 2L of tap water - RH in the closet: 33-35% D90 (19/02/2021): I can definitely see more resin gland forming on buds and can also tell that the calyx are swelling nicely. - temp in the closet: 18-21C - no water - RH in the closet: 33-35% D91 (20/02/2021): I gave 1.75L of tap water. - temp in the closet: 18-21C - water: 1.75L, PH7.5 - RH in the closet: 33-35% D92 (21/02/2021): I checked the trichomes and they are more abundant. It's beautiful to see the buds becoming frostier day by day 😍 - temp in the closet: 18-21C - no water - RH in the closet: 33-35% D93 (22/02/2021): She is starting to show some amber trichomes but not enough. I will let her some days to ripen but she is close to harvest! - temp in the closet: 18-21C - no water - RH in the closet: 33-35% D94 (23/02/2021): I gave 3L of tap water. - temp in the closet: 18-21C - water: 3L, PH7.5, 130PPM - RH in the closet: 33-35% D95 (24/02/2021): Trichomes are all cloudy with some ambers. Due to a timing issue, I will need to chop her this weekend in two or three days. Yesterday I did reduce light intensity to 50% power. She will pass 2 days with this light intensity and then 2 days in complete darkness. - temp in the closet: 18-21C - no water - RH in the closet: 33-35% D96 (25/02/2021): I will not change for week 14 since she is going into darkness tonight for two more days. Trichomes are not exactly where I would like. More ambers would be better but at least they are all milky white with some amber. She's at her peek in term of THC potency. - temp in the closet: 18-21C - no water - RH in the closet: 33-35% D97 (26/02/2021): 24h into complete darkness is done - temp in the closet: 18-21C - no water - RH in the closet: 35-40%

Pronto per la fioritura😈😈😈

Branches are completely full of buds, some are probably featherweight but I didn't really got the chance to do any mainlining on this one because of how weird she grew

- Day 50 The stretch seems to be slowing down a bit now, also seems like she is drinking just a bit less now, so hopefully we see those buds develop nicely from now on. Day 51 Renewed the reservoir today. Starting to get difficult with how big the roots have gotten 😅. I reduced the amount of T.A. tri-part I'm giving as I'm starting to add Big Bud from Advanced Nutrients from now on for the next 2-3 weeks. Day 52 pH got up to 6.4, I Added 1.5 liters of pH 5.5 water, which brought it the pH down to 6.2. Starting to see the bottom fan leaves slowly yellowing as she is using up all those stored nutrients to build the flowers. Day 53 I've noticed the nutrient burn on the leaf tips have gotten a bit worse. I've decided to fill the reservoir with just plain 6.0 pH water for a day to flush out the overload of nutes and will give a lighter mix again tomorrow. Day 54 After having given her just plain water yesterday I've given her a new reservoir with a lighter nutrient mix. Hopefully this will help with the nutrient burn. Day 55 Topped up the reservoir again with 1.5L pH 6.1 water. Seems like the nutrient burn is stable for now. Day 56 I noticed some brown spots on a few leaves on top, I figure they look like calmag deficiency due to the light being to strong. Noticed I was giving 50+ DLI so I reduced light intensity to 45 DLI, see if it stops the spots from spreading. Had to fill up the reservoir again with 1.4L. pH'd water to 6.3 as the water in the reservoir got down to 5.7, which brought it back up to 6.0.

Les hago una defoliación cada 2 días para que los nuevos brotes tengan bastante luz. Empiezo con revienta cogollos a media dosis. He alzado la más pequeña que se queda atrás, la más grande la voy doblando sin problemas. De momento siguen creciendo.

Muy conforme con el resultado, despues de todo lo que les pasó la verdad quedaron muy bien. Chiquitas y poco, pero buena calidad y bien densos

Buenos días gente linda, la planta se recuperó muy bien de los malos cuida qué tuvo cuándo me toco salir de viaje, paso con agua sin medir el PH 3 riegos por mi vecina, pero, por ser una genética muy fuerte y resistente ya esta recuperanda por completo, estoy considerando pasarla a floración al finalizar este mes de julio, pero veremos que tal esta de aquí a esa fecha, saludos a todos, buenos humos 💚

A more difficult week. I've been battling the water consumption on two of the girls. I ordered and hooked up the Blumats auto watering system. I followed the instructions to a T but they don't work on those settings for this type of plant. They must be recommending for succulents cause the girls dried out right away with the settings. So I've been slowing dialing it in the get the proper amount to release. This is the other girl consuming a lot. But with the auto watering system she's holding her own. Dimmed to 80% Here are the lights details: Medic Grow Mini Sun-2 150W LED Model: MN150-022 Spectrum mode: V1 Efficacy: 2.8 umol/J Thanks for stopping by! You can find the light on Grow Diaries: https://growdiaries.com/grow-lights/medic-grow/mini-sun-2-150-watts You can find the light on Medic Grow's website: https://medicgrow.com/

Going to start flushing in about a week or so