Week 10 pics and vids.

We are entering the third week of growth for our FBA 2506 strain from 42Fast Buds, and everything is progressing beautifully. We've recently transplanted the plant into a 10-liter pot, giving it more room to thrive. Even though it's an autoflowering strain, we've decided to experiment with a 12/12 light cycle—12 hours of light during the day and 12 hours at night—to see how it responds. The daytime temperature peaks at 30°C, with an average range between 27°C and 28°C, while humidity is consistently maintained at 60% to 65%. The plant has reached a height of 14-20 cm and is already showing three sets of leaves. We're preparing to start low-stress training (LST) to help guide its growth. In addition, we've begun feeding our beauty with nutrients from Xpert Nutrients, and we’re really pleased with the results so far. The plant looks strong and vibrant, and we're excited to see how it develops over the coming weeks!

Hi all, Welcome to my🍌💜👊 week update Thank you so much for all support on this bananas journey. Much appreciate all your likes, follows and comments. 🙏💚❤️💜 Week 14 Jan 15- Jan 21 Flushing and preparation for harvest 😁 on Jan 15 feed girls for the last time with nutrients. It was reduced dose by 60% 5ltr no runoff. Following morning topped up with 6ltr of ph down and only fish shit. Runoffs PH 6.2. Second watering Jan 18 6tr and 7ltr on Jan 21. It was last watering. Week went very well. No white pistils hairs on Athena for a good while and barely few left on Xena. Many buds have different shapes and colours but all of them are equally hard as rocks, sticky and smelling so deliciously. This week trichomes development was just like I wish for. Just milky and amber in play Status on Jan 22. Mostly of buds 20-50% amber. It's just perfect for my needs🤤 On Jan 21 lights were on for the last time and girls will be harvested after 48 or 72h of darkness. Stay tuned for the final week update! Peace and love brothers and sisters ✌️💚👨‍🌾 Links https://2fast4buds.com/seeds/banana-purple-punch-auto https://plagron.com https://www.biobizz.com/ https://fishheadfarms.com/

I'm happy 🤤

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Hi everyone :-) . This week she continued to develop very well 😊. It was poured twice with 1 l each time. She got training again (topping) and will get it a few more times so that she becomes a real bush :-). Otherwise everything was cleaned and checked. Have fun and stay healthy 🙏🏻 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. You can buy this Strain at : https://www.exoticseed.eu/ Type: Herz Og ☝️🏼 Genetics: Larry OG X Kosher Kush Indica 60 % / Sativa 40 % 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Nutrients : Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8

So far so good. Seems to have taken well to the LST. I did notice the early signs of CO² deficiency and added an organic CO² producer on day 37. Hopefully that spurs the growth. Last frost is in a little over a week. After that I hope the power of the Yukon sun can really give it a boost in its final weeks.

Purple gelato x Unicorn poop | NASC freebie! -Buds are ripening up and coming to a finish -Seeing some amber, notice development in terpene expression and the final swell is looking juicy with big heavy greasy resin heads -Branches are flopping all over, buds are all dense swollen golf ball size from top to bottom -the terp profile is a sweet heavy creamy gas, phunky rancid onion piney like a gymsock, chocolate coffee and fruity kush tones. Strain brings true funk

Flushing her system before harvesting! 6.5 ph water

~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ 8/28/21 😺 We decided to flower these a little earlier than we originally intended to make room for some new strains...I will never mainline a plant this wide again indoors lol, it seemed cool at the time but as you can see it just takes up more space 😑 👎🏻 ...we gave them their first shot of bloom nutrients yesterday (we typically do this 1 week before flower but things came up and we learned we'll need the tent available)...the calcium def spots seemed to have cleared up naturally on the mainline, the other plants are fairly uniform in color (not too dark anymore), and lower growth has been removed...all systems go for flower! 💪😺, I'm hoping for a significant flowering stretch, the new growth after the last mainline topping is approx. 6inches, if this stretches to 12-14inches it should build 8 really good sized colas per side.. thanks for reading! We'll update midweek..happy growing everyone!! ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ 9/2/21 🐱 be careful what you wish for folks..we asked for stretch and we got it..too much in fact, the node spacing isn't good, far too wide with this strain..we lowered both lights in the tent to get this to stop and turned up the fans to really beat on these for a few days..hopefully this will strengthen them, I really would hate to have to steak these later... ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~

The end of week 1 has arrived. We all know how it goes. Just playing the waiting game. At this stage these girls are just working on building their root system. The roots should be hitting the reservoir in the bottom of the buckets soon then they should take off. Got the water pumping and the lights beaming 24 hours a day. Running a low dose of maxigro. Keeping the ph around 6. Will probably be topping them around day 12. Would be amazing if I can get them ready to switch to flower at the end of week 3. But for now I'm just going to check on them 420 times a day hoping they don't die. Might be back next week if they are alive. Happy growing!

Las altas temperaturas han persistido es por esto que el riego ha sido más seguido variando siempre entre fertilizante y agua. Esta vez ya se utilizó Flawless Finish para este último proceso, una vez secándose el sustrato se comienza con la cosecha. Ha sido un buen cultivo. Veremos como termina esto. Seguimos ! 👽💪

-Sponsored by: ORIGINAL SENSIBLE SEEDS-💡VIPARSPECTRA💡-💐GREEN BUZZ LIQUIDS💐-🛠️WEDRYER🛠️ 🏁18/6 hello growerz, with today I start the resumption of diaries on GD. I have 13 new genetics in germination and as many diaries. I start by putting the seeds in water for 24 hours, after a day I will put the two seeds in two different jiffies, one with humic acid from greenhouseseeds and the other with just plain water. Get comfortable and follow the updates 😉👋🦄 19/6 The seeds were divided and placed in two different jiffies, one with humic acid and one with water only. 21/6 after another 24 hours in the dark the jiffies were moved under the lamp. let's start the dances! 23/6 The seed in the humic acid jiffy has already sprouted and is about to open, the brother in the water still nothing visible 24/6 we await the release of the seed in water only 25/6 still nothing on the second suit, I'm starting to worry 30/6 the seed born goes well, the day after tomorrow it will be removed if it is not decided to sprout __________________________________________ Personal advertising (contains affiliate links) __________________________________________ 🦄Highest quality seeds since 1992, a real guarantee! (no affiliate link) ✅https://bit.ly/Originalsensibleseeds __________________________________________ Did you know that Green Buzz Liquids fertilizers are 100% vegan? A complete line of products ready to give the best to each of your plants! Visit the site and see my journals to see how they work 🦄 🤯 And with the code "dreami t" you will immediately receive a 15% discount on your purchases ✅https: //bit.ly/GreenBuzzLiquidsPro __________________________________________ 👀 Are you looking for a good lamp to start with? 👀 🌞Viparspectra has something more than the others, take a look at their site. ⏩ Use "GDVIP" for an extra discount or "DREAMIT3" for an extra 5 %% discount 👀 Search for it on Amazon ✅Amazon USA: https://amzn.to/30xSTVq ✅Amazon Canada: https://amzn.to/38udUVe ✅Viparspectra UE: bit.ly/ViparspectraUE ✅Viparspectra USA: bit.ly/ViparspectraUS ______________________________________________ 🌈 Tired of blowing on your weed hoping it dries quickly? Check out the Wedryer website! You will find a well-made accessory that will help your weed dry in just 8-10 days without the annoying risk of finding mold or other annoyances! (no affiliate links) ✅https: //bit.ly/Wedryer_ ______________________________________________ 📷🥇Follow the best photos on Instagram 🥇📷 https://www.instagram.com/dreamit420/ Backup https://www.instagram.com/dreamit4200/ 🔻🔻Leave a comment with your opinion if you pass by here🔻🔻 🤟🦄💚 Thank you and good growth 💚🦄🤟

4/27. Shes long and lanky but happy and strong. Responded well to the topping. I tucked the top nodes underneath the fan leaves in hopes that maybe her other branches will shoot up. If not, ill tie them down in the weeks to come

🌱 Week 6 Veg Report: Terpyz Mutant Croco Adventure 🌱 Hey, Grow Fam! It’s week 6 in our Terpyz Mutant Mentha de Croco veg journey, and what a ride it’s been! These mutants are taking their sweet time growing, but with each passing day, I’m falling more in love with their quirky, unique beauty. Every plant is a masterpiece of nature—peculiar and stunning in its own way. 💚 Growth Highlights: The slow and steady growth continues to reflect their resilience. These Croco beauties are thriving despite cooler temperatures this week—daytime temps sitting around 18°C and dropping to 14°C at night, paired with 65% RH. They’re proving to be tough little warriors, adapting to the environment like champs! Even though their growth is slower than usual, the details in their leaves are breathtaking. Each plant has its own story to tell, and I’m loving the diversity in structure, color, and patterns. It’s like watching art unfold in slow motion. 🎨 Nutrition Update: This week, I’ve kept feeding consistent with Aptus Holland nutrients—but remember, we phased out the liquid All-in-One formula since the All-in-One Pellets provide everything they need for now. Here’s the mix for this week: • Regulator: 0.15 ml/L – Building resilience and helping them adapt to the colder temps. • Start Booster: 0.25 ml/L – Encouraging root health and giving them a gentle push forward. • CalMag Boost: 0.25 ml/L – Supporting robust leaf growth and preventing deficiencies in these chilly conditions. The pellets are doing a fantastic job of slowly releasing nutrients, ensuring that the plants get a steady supply without any risk of overfeeding. Aptus Holland’s organic-mineral blend continues to impress me with its balance and efficiency. Truly a grower’s best friend! Decisions Pending: Flip or Clone? I’m still holding off on flipping to flower or taking clones. With their slow growth, I want to give them all the time they need to reach a size where I can confidently determine sex. Whether I decide to flip for a week and revert, or take clones for sexing, will depend on how they progress in the next couple of weeks. It’s a reminder to embrace patience in growing. Every strain, every phenotype, every plant teaches us something unique about nurturing life. 🌿 The Beauty of Photography: This week, I brought out my trusty Sony A6000 and Nikon DSLR to capture the intricate details of these plants. From the mesmerizing leaf patterns to water droplets reflecting their surroundings, these frames truly showcase their unique personalities. Why Cameras Over Phones? • Lens Power: Swapping out lenses allows me to get super close for those macro shots, capturing details that even the best phones can’t touch. • Dynamic Range: Cameras handle light and shadow better, making every photo a true work of art. • Depth of Field: Blurring the background helps highlight each plant’s unique features. Sure, phone cameras are convenient, but nothing beats the joy of immortalizing these beauties with the precision and creativity of a good DSLR or mirrorless camera. 📸 Shoutouts & Love: Massive thanks to Terpyz Mutant Genetics for these fascinating seeds, Aptus Holland for keeping the nutrition game strong, Pro-Mix Soil for the rich growing medium, and Future of Grow for providing stellar lighting! A big shoutout to TrolMaster for making environmental control a breeze, and to Grow Diaries for being such an incredible platform to share this journey. Last but not least, a heartfelt thank you to the entire community—followers, supporters, and even the curious passersby. Your love, questions, and feedback fuel this adventure! Final Thoughts: This week has been all about patience, adaptation, and marveling at the uniqueness of nature. Each plant is a little piece of art, and I’m grateful for the opportunity to nurture and share their journey. Keep following along here on GD, and don’t forget to check out my Instagram and YouTube for more behind-the-scenes content and stunning photos. Growers Love to You All! 💚 Let’s Grow, Let’s Share, Let’s Thrive Together! Genetics - Mentha De Croco https://terpyz.eu/products/menta-de-croco-fern-type?_pos=1&_sid=e9237cbcb&_ss=r DogDoctorOfficial Discount Codes @ terpyz.eu - DOCTOR 15% off Nutrition - @aptusholland https://aptus-holland.com/ Led Power @ F.O.G. Future Of Grow https://www.thefuturofgrow.com/en/online-store/BLACK-SERIES-600-p489093171 Controls @ TrolMaster https://www.trolmaster.eu/tent-x As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciloved and i fell honored with you all in my life With true love comes happiness Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so As always, this is shared for educational purposes, aiming to spread understanding and appreciation for this plant. The journey with nature is one of discovery, creativity, and respect. Let’s celebrate it responsibly and continue to learn and grow together! Growers Love To you All 💚

Nice start of flowering

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Best smoke I've had in years!!!!

Now super steady! I need to add way less water than last year (over half a liter a day then), because the eweather is just so cloudy. Just 20°C, max during the day, rarely above and not for long... But it doesn't prevent them to grow fast! Lazarus is a baby that I had put in the filter tank in spring. He spent the autumn and winter in the living room with his bros after I found eggs by september. I thought they had disappeared but there he is! Or maybe it's a joke from a friend...