Good week this plants going steady about 3 n a bit weeks more than four but that's OK giving an update anyway plant going good nice and easy going once I've controlled temps being winter and all plus controlling nutes a bit more this plant seems to need more trace elements good learning loving it.

End of day 77! Harvest time :)

Girls jus doing their thing living their best

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Though I have not used any new grow techniques of yet, I was taken aback when I found out that, my LST turned Main-Lining, when I twirled one of the stems around the protruding twig. Loving how she has taken shape, I thought they would never grow but look at her maturing at Godspeed alike her 5 friends. This coming week, I’ll be increasing my nutrient dosage from 125ml to 250ml however keeping my daily watering at 1lt.

Exsept some minor feeding problems the plant look great . smell start to get a bit noticeble as the weekea go by , the buds getting fat and frosty and its fun to watch ! i think i gonna stop with the nutrients because the plant seems to have more then he needs and the harvest is 2-3 weeks ahed .

Another week gone for this plant and she’s took really well to the training implemented last week. Knuckled both of the main branches last week and kept the biggest branch tied down ans as you can see she’s shooting up from doing so. Should get few more tops now and bush out. She’s come on lovely these past few weeks after mowing munched on by slugs and I personally didn’t think would recover from it but I was so wrong! Ones she gets strong again from the knuckling I will tie all heads down to get more light into the middle. Next time down will give her some bio grow but this week shes had plenty of rain. Been ate a little by slugs so put some copper tape around stem to help keep them off. That’s all this week happy growing 🌱

Asi van las cosas hasta el momento; Dia de actualizacion en la semana: 4/junio/20 (dia 29) +Se agrega malla Scrog con 49 Espacios (Se podra completar?) El día 04/06/2020 se han regado 3 plantas en macetas de 11 litros: X2 Semillas Auto Lemon Kix= 1L [1mlKnactive+3ml/L ATAGrowth-C+3mL ATAFlower-C] PH 6.2 / EC= X Tº=23 X1 Semilla Auto Tutankhamon= 1L [1mlKnactive+3ml/L ATAGrowth-C+3mL ATAFlower-C] PH 6.2 / EC= X Tº=23 *El día de hoy se han regado 1 planta en macetas de 7 litros: X1 Semillas Auto Lemon Kix#3 = 550 ml [1mlKnactive+3ml/L ATAGrowth-C+3mL ATAFlower-C] PH 6.2 / EC= X Tº=23 El día 08/06/2020 se han regado 3 plantas en macetas de 11 litros: X2 Semillas Auto Lemon Kix= 1L [1,5mlKnactive+3ml/L ATAGrowth-C+3mL ATAFlower-C] PH 6.1 / EC= 1.17 Tº=23 X1 Semilla Auto Tutankhamon= 1L [1,5mlKnactive+3ml/L ATAGrowth-C+3mL ATAFlower-C] PH 6.2 / EC= 1.17 Tº=23 *El día de hoy se han regado 1 planta en macetas de 7 litros: X1 Semillas Auto Lemon Kix#3 = 550 ml [1,5mlKnactive+3ml/L ATAGrowth-C+3mL ATAFlower-C] PH 6.2 / EC= 1.17 Tº=23

So they still have more ripping to do. I only harvested the main colas on 3 plants still a lot more bud to go at least the same amount I'll update total weight etc. When it's all finished cant wait to test it out.

Day 21 and the flowering tent went kaboom big buds spliff strawberry are taking off .still no smell at least I haven't smelled anything but the carbon is running full blast heavy humidity here in Canada this week lots of rain

Beautiful plant, smells amazing

La planta ya empezó su etapa de floración 🔥💪 En este caso hemos aplicado una pequeña cantidad de Monster Bloon de Grotek pulverizado para la creación de tricomas de forma foliar y con ello absorba todos los nutrientes posibles Hemos bajado la temperatura 2°C y la humedad 5% para su floración La malla de scrog esta haciendo muy bien su funcion y esta respondiendo fácilmente al entrenamiento. Esperemos que siga en esta línea 👌

Week 6 of flowering, and things are heating up—literally with the scent! 🌬️💨 Both plants have significantly increased their aroma, and every time I open the grow box, I’m greeted with a strong and delightful smell that fills the space. The non-LST plant is much further along in its development. Its buds have progressed beautifully in color, and I’ve already noticed the first amber trichomes appearing. 🍂✨ This, along with its overall appearance, is signaling that it’s nearing maturity. Because of this, I’ve started flushing the plant with clear water to prepare it for harvest. Speaking of the LST plant, it’s about a week behind. 🌱 While it’s not as far along, it’s still thriving with strong trichome production and a stunning Lemon Haze aroma that’s sharper every day. 🍋💚 Both plants are doing great, and the differences in their timelines make this grow even more interesting. I’m excited to see how the LST variant catches up in the coming weeks! 😊🌟

Honestly not much to say about the dry and trim, which is definitely a good thing. 12 days hanging in the tent, and the plants were ready to trim. me & the Mrs. absolutely sailed through it. Stores like a dream, and smokes the same. Not a crazy amount of flavour, but the aroma that it has is lime and cream. And the taste is more earthy and spicy. Loving and savouring every bit of it. Sorry I’m very late with this, but I’ve been busy getting my next plants started! You should see them come up soon, as I’m quite behind in my posting. I’m very happy! 🤟💚

La mia LSD-25 sta avendo problemi per una sovra irrigazione ancora dopo la prima settimana e le foglie più giovani sono tutte arricciate verso il basso…ora dalla scorsa settimana ho preso appunti pesando i vasi giorno per giorno segnando anche l’orario. Spero che riesca a riprendersi senza troppi danni ma è da vedere come è messo l’apparato radicale, vediamo come si comporta i prossimi giorni, ieri ho aggiunto 20g di top dress di Dogma Organics composto da guani misti e ammendante vegetale misto, la componente minerale è composta da kierisite e fosfato naturale tenero! Una bomba per la fioritura.

Fantastica

🌱 Week 1: The Journey Begins! 🌟 Hey Grow Fam! 🙌 We’re off to an epic start with our TrolMaster adventure, and I’m already feeling the love for the setup, the seeds, and the growth happening in the room! So, let’s dive right in and get into the details of this exciting week! 🌿 Germination Update: Our first Gorilla Melon seed from Fast Buds didn’t sprout, but no worries—round two was a success! 🦍🍈 The second seed has germinated and is looking full of potential. I’m throwing in a Sweet Seeds Papaya Zoap into the germination station too, so we’re keeping this exciting lineup going strong! Germination is such a key part of the journey, and having a healthy start is what it’s all about. 🔧 Room Build & TrolMaster Setup: The build is coming along beautifully, and I gotta say, the TrolMaster system is blowing my mind! Everything’s under control like magic 🎩✨, and the TM+ app? Wow, just wow. I mean, I can literally be in the bathroom and still keep an eye on the plants—check all the metrics, adjust settings, and fine-tune what I need, all from my phone! 😂💧 This level of control is next level. I’m loving how smooth and seamless it makes managing the room. The gear is just unreal, and seeing it all come together is truly something special. 🌱 Nutrition Talk: As for feeding, we’re starting light but smart! I’ve begun with 0.15ml of Regulator and 0.25ml of Startbooster per liter for now. I always believe in starting easy and ramping up as the plants develop. These early stages are crucial—balance is everything. For the time being, I feel this is more than enough to keep the babies happy and healthy. 🌿💧 The Regulator is working wonders in maintaining plant structure and health, while the Startbooster is fueling the early root and vegetative growth, laying down a solid foundation for the weeks to come. It’s about getting it right from the start—too much too early can be a killer. Keeping it steady with precision, that’s the goal! Big Shout-Outs: To the amazing team at @TrolMaster, @Fast_Buds, @Sweet_Seeds, @AptusHolland, and all the incredible sponsors—I’m beyond grateful for the support! 💚 And to my GrowDiaries fam and followers, you guys make this journey what it is—thank you for the love, and I’m so honored to have you along for this ride. 🙏 We’re just getting started, and I can’t wait to show you what’s next. Stay tuned for more updates, epic builds, gear reviews, and plenty of insight. Don’t forget to follow me on GrowDiaries, YouTube, and IG for exclusive content, and let’s make this grow the best one yet! 🌿🔥 Growers Love to All 💚💚💚 P.S.- A deep dive into the Full Spectrum Quantum Sensor (MBS-PAR): The Full Spectrum Quantum Sensor (MBS-PAR) is an essential tool for any serious grower looking to optimize their light environment. This sensor measures photosynthetically active radiation (PAR), which refers to the spectrum of light that plants actually use for photosynthesis—typically between 400 and 700 nanometers and more depending . What makes the MBS-PAR sensor special is its full spectrum capability, meaning it accurately detects not just visible light but also far-red and UV light—which are crucial for maximizing plant growth, development, and flowering. With this tool, I can monitor the exact intensity of light my plants are receiving, making sure they’re getting the optimal levels for every stage of growth. By using this sensor in my grow room, I’ll be able to ensure that the light distribution is uniform across the canopy. Plus, it allows me to fine-tune the light output from my ThinkGrow LED setup, ensuring my plants are thriving under the best conditions. Whether it’s increasing light during the vegetative phase or fine-tuning it for flowering, the MBS-PAR sensor helps me keep everything on point. And the best part? I can monitor it all through the TrolMaster app, ensuring I can make adjustments whenever needed—even if I’m not in the grow room! This level of precision means healthier plants, better yields, and maximum efficiency. It’s a game-changer for taking the guesswork out of lighting and getting closer to growing perfection! 🌱💡 Growers Love to All 💚💚💚