This was an easy week with flushing the 2 B Blunt and Wreck'd Nuts so that I can take them both down. The Wreck'd Nuts just did not recover well from the lock out issues it was facing. It all came out of nowhere and got kind of serious even with a solid flush. That strain is a bit more picky with the nutrient balance than the others were. Leaving the Sex Melon and Rainbow Candy for next week. They still have some beefing up to do.

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

The second week of vegetation is over and watching the plant grow is a rewarding experience for me. Now that the plant is well-established, it is time to begin training it. This involves LST to ensure it reaches its full potential in this case. I look forward to discovering what this plant will become 😋😊

7 Woche

6 SEMANA (3 FLORACION) - ESTA SEMANA SE A HECHO UNA LEVE DEFOLIACIÓN PARA EVITAR MAS HUMEDAD - LAS PLANTAS SIGUEN SANAS Y SALUDABLES - YA NO PRESENTA PROBLEMAS DE CALOR NI FRIÓ

Week 8: Having some issues with my lower leaves at the moment , but the rest of the plant is really good. We are also being hit by a big heat wave in here , temps outside reaching 40C, but I’m managing it well for now. I will probably have big issues when the drying comes - open to ideas 💡

In week 7 the 12 plants have filled out the space inside the HOMEBOX grow-tent very nicely and therefore its time to switch to flowering. I change the timer, which I put infront of the supply of my SANlight EVO4-120 LEDs, to 12/12 hours of light/darkness. This will induce the flowering of my babies and they can start to develop buds. The BIO NOVA nutrients work very well, which can be seen in the nice healthy green color of the plants. I follow the SOIL-CULTIVATION chart from the BIO NOVA website, but use only half the recommended dose, because my tap-water already has a very high EC-value of 0,8 when it comes out of the faucet. Since I have used REGULAR seeds for THE PURPS from BC-BUD-DEPOT, I have to check for males on a regular basis. Two plants show clear male pollen-sacks already now (see above in video) and have to be culled right away. I cut the plants off at the main stem right above the soil and take away the plants and throw them into the trash right away. This way I make sure NO pollen can mature inside the male pollen-sacks and be released later and inseminate my babies on accident. In the places of the male PURPS plants, I put a MAC and KK-plant into the AUTOPOT-system, so all 12 placed are filled again. Now its time to wait until the first flowers start to appear. 👍😎

Week 1 Day 1 - 8/12/2023 1st Water change Day! Such a special time it is when you remove the little bit of Nutes that you gave them as an appetizer and you give them their first real meal. Added 39 Gallons of Water to my system SILICA= .5mil/Gal = 19.5 = 20mil Root Drip = 1mil/Gal = 39mil Cal Mag= .25mil/Gal = 9.75 = 10mil FLoraMicro= 3.0mil/Gal = 114mil FloraGro = 2.0mil/Gal = 78mil FloraBloom = 2.0mil/Gal =78mil ORCA= .5mil/Gal = 19.5 = 20mil Week 1 Day 2 - 8/13/2023 Everything is looking good the roots are making their way to the water and the new grow is looking nice and green. Week 1 Day 3- 8/14/2023 Everything is right on track, they are looking beautiful and in the praying position all leaves happily lifting towards the light. Week 1 Day 4- 8/15/2023 A little worried today her birth Twin the BA I am growing out is looking great and is raised towards the light and this one is just slightly under.. Will keep an eye on Her. Week 1 Day 5- 8/16/2023 Walked in and the humidity was under 60.... ohh noooooo.. So I added 2 humidifiers to the tent and attached them to my InkBird controller which is set to 62. Also looking at the roots and she has some poking out the bottom but just not in the water yet.. Luckily we are set for 14 days before next water change so the system will stay stable and her sister already has roots in the water so she should only be a day or two behind.. we will just keep tracking but she is delayed. Week 1 Day 6- 8/17/2023 Roots in the Water!!! Huston, we have a successful launch. This grow is on! Humidity was a little low this morning so I refilled the humidifiers. Other than that the temp looks great, the PH looks good, the PPM looks good the plant is in the praying position and all damage from the little drowning from over filling the cloning machine seems to have been fixed. Happy Happy. Week 1 Day 7- 8/18/2023 Yay.. week 1 in the books, roots in the water growth has started. Everything for growth and environment is looking good and on track, there are a couple of mutations with this Lady will keep an eye on those leaves. IMO this grow is going A lot during this week 1 then week 1 of the last grow when I had them drowning. Really excited on how this grow is going to come out.

Ready to be chopped and dried. Super frosty Pheno. Not usually a fan of foxtail’s but this pheno make’s it look lekker.

Lamp Brightness: 100% @ 16" - estimated 914 PAR (top of GMO 2/back left) Waterings only, daily crop steering - reduced water temp + dry down periods IPM once-a-week visual inspection - no foliar applications notes: harvest likely next week (after 12 weeks flowering) trichome check - showing little signs of amber looks like they could go longer but another week I feel is sufficient smell has largely stayed the same for a few weeks if anything just more complexities

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Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 22 since time change to 12/12 h. Hi everyone . short update on the mini plant :-) it is starting to bloom nicely and i leave it as an experiment :-). There was also 1 g GHSC enhancer per liter. It was poured 2 times with 1 liter this week. Have fun and stay healthy 🙏🏻 You can buy this Strain at : https://www.zamnesia.com/de/5165-zamnesia-seeds-kalini-asia-feminisiert.html Type: Kalini Asia ☝️🏼 Genetics: Black Domina x Purple Kush 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

Riego solo con agua, preparando la

Welcome to week 6 of flower for this lovely project! Last week was exciting this week should be even more exciting! With the ladies beginning to put on serious weight it's only a matter of time before I'll have to install a second net to help support the branches. With this being about the halfway point I'm excited to see them at the finish line! Huge shout outs to @MarsHydroLED for making epic lights and tents for people to grow in! Huge shout outs to all my followers and people who stop into the diary alike! Keep inspiring to grow! -The Projexx Day#36F Creamy Cereal is PUMPING frost now with its undeniable smell of fruit loops and milk. Day#37F Ladies are putting on mass and quickly! Day#38F Macmelonz continues to stretch and stack but looks like its nearing the end for her stretch phase. Hopefully she pumps HUGE kickass flowers for me. Day#39F Flowers are almost the size of a soda can now. The trichomes on these plants are wild like trichomes over trichomes. The flowers have really picked up on the aroma department as well very please with the plants. Day#40F Raised the light abit, Flowers are starting to display colour in them now can't wait to see them in a few weeks! Day#41F Pictures N/A. Ladies are still beefing up flowers some of the pistols are starting to turn. Day#42F Ladies are still putting on mass daily. Lots of lovely unique smells coming from the plants. Recap: Things went well this week , the ladies really put on some mass and all the flowers are hard as rocks! I will be taking away leaves over the course of the coming weeks to ensure the airflow to the flowers. Excited to see what the coming weeks bring!

June 14th : Didn't have a ton of time for photos tonight so I did a short video of the tent! Week 4 flowering is looking great! They are bouncing back from their defoliation with no problems! June 15th : Watered tonight, Seeing some Good Growth on the bud sites. I think all this love I've been throwing at them is working! June 16th : Was out of town and got home too late to take pictures. 😭 June 17th : Starting to wonder if I did something wrong. Been looking around at diary's and like everyone else has giant buds already formed by the end of the third weekish.. And mine aren't all that impressive.. Guess it's the cheap ass Amazon lights.. Fml..😕 June 18th : Welp now I don't know what to think. Lol Buds seem to be getting bigger every day.. Hope some of these large main stems start stacking bud sites. Come on girls!! Fat shout out to Crop King Seeds for some testers! 🙌 7 Dark Angel Feminized, 7 White Cookies Feminized, 7 NYC Diesel Autoflower, 7 Revolver Autoflower. June 19th : Man the Crystals are starting to really show all over these leaves.. Beautiful just beautiful. Didn't do anything tonight other than admire them for far to long. Feeding tomorrow! June 20th : Last night of the 4th week of flower. Did another nutrient feeding tonight at 2/3 recommended strength. Seems like they are starting to stack up on the bud sites. Loving how it's looking. Gonna start with some macro photo's next week I think. Loving this adventure!

13.4. - Slowly switching to bloom ferts. Also added Bud Candy to the mix. I had to defoliate a bit more as the humidity is raising during the night, even with dehumidifier. Watered 💧 15.4. - Seems like plants like the new mix. 4 of 7 plants already in flower. Jack even started to form the first buds. Watered 💧 17.4. - Plants look nice, buds are forming and each day I find more. Unfortunately, one plant (Gorilla) is probably some bad pheno and has a lot of thin leaves. However, it also starts to form some buds sites so nothing is lost! Watered 💧 19.4. - Week wrap-up: I'm totally out of free space. Plants took over the whole growbox and they're thriving ! However, I'm spotting some signs of nutrient burn/deficiency, so I gotta change up the mix again. I guess I will try to remove the Bio Grow. Watered 💧 13.4. - 19.4.2024

5/3 Week 6 Last day for Grow nuets looks like stretch is stopping. Will confirm in the AM. Also be ending the round of PK tomorrow. Lights at full power and set to @16 inches to start. Leaving the MH bulb in place a couple of weeks Very light defoliation just to keep middle of the plants open, this strain does not need much at all. 5/4 Ending Grow nuets as stretch is playing out so no further need of the Nitrogen, lot in the pot still. Last day for the PK on this go round. Next time we grow this strain going to look at alternate methods of training, FIM is the likely candidate. 5/5 Back down to just Bloom A/B nuets Stretch slowing down as Baba and Sally reach the same height as Athena that started first. Everybody seems happy even the little one 5/6 Moved up the lights, was seeing some light stress on the girls, leaf praying. Bumping up the Bloom nuets for now see how it goes, getting close to next round of PK booster waiting for the cotton balls to develop sufficiently. Gave all a shot of Fish Sh!T this morning 4ml/gal. Pretty much all of it 5/7 Pics Raised lights further still have some praying leaves. Tomorrow should be PK day as the cotton balls should be formed by then. 5/8 Looks like the last the stretch has finally ended on Baba, thought she was going to be a good kind of problem but didnt have to raise the lights today so there is that. Have to be careful with these lights and watch for light stress more diligently than I have so far. HID at 600w is 17 inches above the rear plants, TS at 150W is 18 inches above the tallest front plant(Sally), seems to be working. Added KoolBloom back into the feed, 2ml/gal, the buds have become little cotton balls so its time. Cleaned up the lower end of the plants, was getting snaky down there. 5/9 pics Ending the week by doing a little bending on the tallest branches on Baba, they were a full two inches higher than any other. She is still growing a little but nothing to really notice.