I started germination of this Gold Bar Kush bean on 29/12/2020. I pre moistened my rockwool cube with ph balanced water to 6.4. Made sure the plug was just damp and not soaked. Using a small wooden dowel I increased the size of the plugs pre made hole. Than I sowed my bean into the hole. Ripped off a small piece of rockwool and mulched it up. Lightly filled the hole in with the mulched rockwool. Than stuck the plug into a misted humidity dome, to complete germination. Shouldn't take anymore than 4-5 days to see a sprout. Once I see some cotlydon leaves bursting to the surface. I will get the plug planted into a 1 gallon pot. Plus get this lady situated into her new home. Cant wait! Some background information on my first run with GBK. It's a VERY robust variety packing hella branches, hella quick. Prime candidate for continues ongoing lst work. To maintain a clean,uncongested even canopy. She can become a jungle quick if left untamed. Shes very easy going in the nutrient department. Not hard to keep this one happy. Packs on lots of weight everywhere towards end of flower. Branches most definitely need some support or they will come crashing down. The flavor and aroma from this variety sre majestic. The intense indica effects are heavenly. Not hard to guess why it's back in the grow room for round 2.

These bids are looking nice and fat, a very nice og Kush smell and vigorous plants, this week we are flushing and i think that somewhere around next week the plants might be ready but of course i will review the tricomes 😁

Time to flush this beautiful plant. the buds are dense & solid with amazing colors.

Hello fellow growers, I haven't realized that is has been 2 weeks already so going into week 7 with the amazing bubblegun. There are quite some changes when you compare the pictures from two weeks ago it looks like there wasn't any defoliation done at all the tent is packed with big leaves and happy plants with the defect are still a little behind and are not as full as the others and the leaves look a bit weird but we will see what the fututre brings. No scrog netting this time but the next time i will use it again cause i like the way that it effects the plants growth. i lowered the lighting by about 20 cm's and i will do some defoliation in the following week no changes in the nutrients but starting next week im going to add Epic Blast the flower booster from hy-pro That is all for this update talk next week Cheers, Nibameca

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Harvest day 71 since the time change to 12/12 hrs. Hey guys :-) Finally the time has come . The lady was harvested. After the trichomes have been checked (70% milky 30% amber) as always, it was left in complete darkness for 48 hours before it was neatly trimmed by hand. After trimming, she was put back in the drying tent on nets. There they are allowed to dry for the next 8-12 days at 62% humidity before they are put into the jar to ferment with 62% boveda packs. After about 4-6 weeks in the jar I will swap the 62% boveda pack for a 58% boveda pack where it can be ready for another 4-6 weeks to enjoy :-). As always, the remains of the leaves are used to make Ice o Lator and oil. Of course, as always, there is a final update during the fermentation process. Until then, I wish you all a lot of fun with this update. Stay healthy 🙏🏻 and let it grow 👍. You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. You can buy this Strain at : www.Zamnesia.com Type: Runtz ☝️🏼 Genetics: Zkittlez x Gelato 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205 W Soil : Canna Coco Professional + ☝️🏼 Nutrients : Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.4

EC Problem not solved yet. Clear water: 0.67 With nutrients: 2.5 Just trimmed some leafes. Vanilla Kush is slowly getting better but deep cheese is getting really huge.

She has slowed right down and isn't packing on much weight. And possibly started to hermie :( got most of them out and no other plants in the room so no worries about pollination. Got to say I'm a little disappointed but I'll be flushing tomorrow and maybe one more flush next week so hopefully all isn't lost

03.22.23. Start Week 3. Today i started the Low Stress Training on the plants. I first carefully start to bend the plant kinda massaging the plants and making the plants softer and easier to bend down. I then tie the base on one end to keep the plant from snapping, and bend the plant the opposite direction and tie it down. I will continue this daily until flower starts to create a perfect even top canopy. End of week Review. All 3 plants seem to be reacting well to the nutrients and LST they are getting. Also the few leave trimmings seemed to go well with them all. I have officially named the plants. There names are Megan, Lucille, and Alien. Very excited for these plants to start there flowering stage

11/29 - Day 57 - Start of week 9. Fed the plants today with cal mag, base nutrient trio (micro, grow, bloom), and MKP. The plants appear to be very happy and healthy. Flowers are developing nicely on Blanche and Pinky, but Bleu is still showing only a few pistils at the bud sites. I'm wondering whether I should adjust the light schedule from 18/6 to 16/8, 14/10, or even 12/12. Will reassess at the end of this week. 12/02 - Day 60 - Fed nutes ppm=700 PH=6.5 w/ recharge and molasses. 12/05 - Day 63 - Fed nutes ppm=825 PH=6.5. Pinky and Blanche are starting to fatten up. Bleu looks as though she's just starting to kick into another gear, but will probably finish at least 2 weeks after the other two. Excited to see the eventual yield considering she will get the entire tent to herself during the final few weeks, is significantly larger than the other two plants, and is drinking/eating more.

D50/F06 - 20/05/23 - Nothing to report D51/F07 - 21/05/23 - Added water and nutes EC=1.1 pH=5.7 D52/F08 - 22/05/23 - Nothing to report D53/F09 - 23/05/23 - Added water. EC=1.1 pH=6.4 D54/F10 - 24/05/23 - Added water and nutes EC=1.0 pH=6.3 D55/F11 - 25/05/23 - Start the week out. I set up a system to feeding Nora during my stay out (I'll take some picture about...) D56/F12 - 26/05/23 - I leave today, 1 week away

Haar laatste uren zijn gekomen. Wat een geweldig prachtige bloemen heeft ze voortgebracht. Echt super! Ik zal haar zondag 6 juli omhakken en in haar geheel ophangen. Foto's zullen volgen dus hoe het in de gaten. Wordt vervolgd......

it has really developed very well. it has grown another 20cm. and the flowers are now really coming into their own and are getting thicker and thicker. because of the heat, it has been given calm-a-mag and acti-vera by biobizz. and of course now also bio-bloom.

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Día 78 (06/01) CBD Auto 20:1 #1 - El amarilleo continua... esperando que haga efecto el SILICIUM FLASH 💥 OG Kush Auto - Empieza la ventana de cosecha: Reviso los tricomas y solo hay algunos nublados (el resto trasparentes), pero creo que acabará en una semana más como máximo! Día 79 (07/01) CBD Auto 20:1 #1 - Parece que el amarilleo se frena un poco. ¿Será suficiente y formará unas buenas porras? OG Kush Auto - Riego 750 ml. Está consumiendo mucha agua en este tramo final! 😍 Día 80 (08/01) CBD Auto 20:1 #1 - Está recuperando el verdor! El SILICIUM FLASH está haciendo efecto! OG Kush Auto - La planta no para de beber y las ramas se doblan por el peso de los cogollos! 😍💥 Día 81 (09/01) CBD Auto 20:1 #1 - Los cogollos empiezan a coger mayor densidad. Según mis cálculos, le faltan 17 - 24 días OG Kush Auto - La senescencia sigue avanzando pero los tricomas aun no están listos (50% nublados). Espero cosechar en el día 84 aproximadamente Día 82 (10/01) Busy day Día 83 (11/01) CBD Auto 20:1 #1 - El SILICIUM FLASH ha hecho efecto y empieza a retomarse el verdor en las hojas! Que maravilla! OG Kush Auto - Los tricomas están mayoritariamente nublados y algo ámbar. Mañana la cosecha! 😁 Día 84 (12/01) CBD Auto 20:1 #1 - Los cogollos empiezan a apilarse y a engordar 😁 OG Kush Auto - Día de cosecha! 😍💥💨😁 FastBuds 15% DISCOUNT code "NONICK" 2fast4buds.com @fast_buds_official_ @fastbuds.official 💦 BioTabs 15% DISCOUNT code "GDBT420" biotabs.nl/en/shop/ @biotabs_official 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE @promixmitch @promixgrowers_unfiltered 💡2 x Mars Hydro FC1500 EVO Led Grow Light (2024 NEW FC 1500-EVO Samsung LM301H 150W LED) - https://marshydro.eu/products/fc1500-evo-led-grow-lights/ - https://www.amazon.de/dp/B0CSSGN5D8?ref=myi_title_dp

Honestly not much to say about the dry and trim, which is definitely a good thing. 12 days hanging in the tent, and the plants were ready to trim. me & the Mrs. absolutely sailed through it. Stores like a dream, and smokes the same. Not a crazy amount of flavour, but the aroma that it has is lime and cream. And the taste is more earthy and spicy. Loving and savouring every bit of it. Sorry I’m very late with this, but I’ve been busy getting my next plants started! You should see them come up soon, as I’m quite behind in my posting. I’m very happy! 🤟💚

🍀25/01 - Empieza su séptima semana en etapa de floración. 🍀Ya empece con su lavado de raiz, los pistillos ya estan de color ambar. 🍀Se encuentra muy bien, no tuve ningun problema con ella en toda su etapa. 🍀Esta cumpliendo 83 dias de vida desde su germinacion. 🍀Va a estar 1 semana en lavado de raiz y la podo. 🍀Su ph se encuentra en 6.0, en estos días voy a ir bajando gradualmente hasta llegar a un ph de 5.8. 🍀En su primer lavado le agregue 30L de agua aproximadamente con 10ml de Flawless Finish. La maceta que estoy utilizando es de 10L. 🍀En estos dias estare subiendo mas imagenes de como viene. 🍀🇦🇷😶‍🌫️Podes seguirme en Instagram como @BruWeed_arg🍀🇦🇷😶‍🌫️