So far so good, I guess. I think magnesium deficiency in some plants but I’m not quite sure yet. I have plan to feed some nutrients in next few days. And I’m really looking for some suggestion and that would be my pleasure.

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Dette er min stolthed pt hun har nok ca6uger igen hun bliver mere og mere fyldt med buds og masser af fimre hår!mit nye opset er det bedste jeg har gjort for mig selv og mine piger elsker det;) så keep on growing

Girls have settled into summer camp nicely. Worries about not taking the transplant diminish more each day and I am gaining more confidence in strong roots with each passing day as well. I had to build a supplemental lighting system for the greenhouse. Here at home we are experiencing a wetter than usual summer so far and with that brings clouds. So I needed to do something. So I built a new greenhouse light. It cranks out some light. Currently at canopy I’m measuring around 600 PPFD. Plants are loving it. All three plants sexed and all are females. Didn’t think otherwise. So the formula 707 is performing better than expected. Water retention is crazy. Watered the plants on Sunday with a gallon each , today is Thursday and no water needed. Going to try and get to Sunday for a whole week between watering. *** update, added a short video from 7am this morning. The girls really do like the homemade light.

Moby 1: La Moby esta a sus 8 días en preflora en exterior y ya obscurece tipo 18 y 30 y amanece después de las 7 aveces esta muy fresco s la noche ahora estos días de 3° A 16° la temp.Se adapta muy bien veré q tengo para incentivar la flora ya tenia pelos q de.ostraban su sexo y sigue bie. Les aplique Sílice quedo con un ph 6.4 aproximadamente,para que estén más fuertes y puedan resistir algunos cambios de temperatura, Moby 2 : Está vegetando pasa 4 hs de noche y lo demás en el sol y bajo luz blanca para completar,todavía seguirá vegetando y quisas le saque algún esqueje más.. Pd: Se ve en una foto atrás el techo cono de alromb4a de auto qué uso para cubrir en la noche la planta simple pero más el aislante en la maceta se cubren bastante.

Couple days after the big defoliation at the end of week 2 flowering and all is looking good. Wasn’t sure if I took too much but it seems to be exploding still and I’m still tucking a bit but mostly We are letting them come up a bit above the screen to start forming our canopy. Looks a bit uneven now but all bud sites are exposed within 10 inches of the canopy and everything is being kept clear as possible. At the end of the third week she slowed down stretching and we did one last light defoliation. Well that’s it for this week, all caught up! See you next week! Cheers 💨

Hey everyone!!! Start of week five. Everything is starting to come along. Everything is gping fine in the garden. Still super cropping some of the branches on cc2 to keep it even. Added some open sesame to this weeks feed. 😎 cheers Update 11/15 hey everyone back with an update. Fed the girls this morning, and the first thing i noticed was the sweet aroma. 😁😁 these girls love water amd nutes i feed them about a gallon of water every 2 to 3 days. Lots of pistills starting to come in as well😁😁😁😁 i also lsted and leaf stripped as well! Cheers!✌️✌️

I upgraded her from her old 2.5gal plastic pot to a 7gal fabric pot and she’s doing a lot better. The roots were experiencing damage and I had to remove a chunk of em but it’s a lot healthier now :)

5/11 Decided to harvest all of Amnesia at 11pm, a couple of hours of watering. This is a lot of bud. Good thing I installed a new row in the drying tent! No weighing until I trim, cuz these stems are fat. 5/25 Looong, slow dry at high 50% humidity. After drying and trimming we're at 133.4 grams, or 4.75 oz! Time for curing in the grove bags!

She grew 17cm this week. Over doubled her height. Lets see what she does in the next couple of weeks. I note she got a LOT bushier. Also her leaves are VERY broad and indica like for such a sativa strain! 😶

90x60x140 (Mars Hydro) FC-E4800 (Mars Hydro) Easy2go Aquavalve5 (Autopot) Living Soil (Demetearth) Bruce Banner (PEV Seed) Gorilla GG4 (Ganja Farmer) Cream Caramel (Sweet Seed) Gorilla Gelato (Ganja Farmer) Blueberry (00 Seed) Kalini Asia (Zamnezia) Biscotti Mintz (Barney's Farm) Blackberry Cake (Sensi Seed) Amsterdam Amnesia (Dutch Passion) Gelato Cookie (Ganja Farmer) Purple OG Punch (Ganja Farmer) Sweet ZZ (RQS) Rainbow Road (Paradise Seed) Substrat ~50L: 30L Super Light Mix Biotechnologie 18L coco 2L perlite 1,7kg lombricompost 350g guano de chauve-souris 350g Zéolithe 350g Basalte 170g guano vers de farine 170g biochar 0,7g Endomychorise glomus intraradices 0,7g Bacillus Amyloliquefaciens Paillage de luzerne alfalfa Arrosage avec 2ml/L de mélasse de canne 1x par semaine Pulvérisation avec 1% d’huile de neem jusqu'à la 2eme semaine de floraison

Week 12 for Black Lebanon by SSSC She's really filling out nicely compared to last week I've had to bend some of the tops that were getting taller than the rest but nothing crazy. I went through a period of barely feeding her... well this week she's been fed 5-7L of water plus heavy heavy sprays every night😂 so a bit of a change up she doesn't seem phased either way. Currently having issues with crickets... keep eating my leaves not sure what to do about it if anyone can suggest anything? 😎

when I came today, I didn't recognize her, she was eaten by snails, but she put out new branches, which grew rapidly :) she is already showing signs of rain :) or it is already the beginning of flowering:).

**Encontrarás la traducción a español al final de la descripción** From/Desde: 15/03/19 || To/Hasta: 21/03/19 From day/Desde día: 22 || To day/Hasta día: 28 You can find the Gorillas Diary here: ** Podéis encontrar el diario de las Gorilla aquí:** https://growdiaries.com/diaries/25675-makingmoney-with-gorilla-mm-vs-gorilla -----IMAGES & VIDEOS----- Video 1: Exactly the same video as 2 but accelerated +300% to fix it to 1 minute for instagram. Video 2: 4 days (25~28) TimeLapse, 1 image each 60 secs @ 30 fps Video 13: General video of the 4 plants and it's trainings, i'm sorry for being so repetitive with the music. Video 16: Video of the main Money Video 19: Video of the secondary Money Photo 22: Last main branches toppings -----WEEK SUMMARY----- This week i will just say, an image worths more than a million words... so now let's talk about a video 👊 -----WATERING CALENDAR----- 18/03/19 - 400 ml with all "LAST" week nutrients @ 0.95 E.C. PH5.9 (i had some rests from the last watering) - Also i applied a foliar irrigation with the same mix as the last week 20/03/19 - 1.000 ml with all week nutrients @ 0.9 E.C. PH5.9 *****ESPAÑOL***** -----IMÁGENES & VÍDEOS----- Vídeo 1: TimeLapse, exactamente el mismo vídeo que el 2 pero acelerado a un poco más del 300% para ajustarlo a 1 minuto para Instagram. Vídeo 2: 4 días (25~28) TimeLapse, 1 imagen cada 60 segundos @ 30 fps, vídeo increíble en el que se ve com responde la Gorila al Man Lining, en aproximadamente el minuto 2 podo y entreno un poco a la otra Gorila y la principal de las Money Makers, al final del vídeo podaré la última Money Maker. Vídeo 13: Vídeo general de las 4 plantas y sus entrenamientos, siento la repetitividad de la música entre los vídeos. Vídeo 16: Video enfocado a la Money principal Photo 10: Los cortes nombrados (Top&Bottom) de la foto 11 Photo 22: Los toppings de las ramas principales. -----SUMARIO SEMANAL----- Como una imagen vale más que mil palabras, ahí os dejo varios vídeos 👊 -----CALENDARIO DE RIEGO---- 18/03/19 - 400 ml contodos los nutrientes de la "ÚLTIMA" semana @ 0.95 E.C. PH5.9 (me quedaba un resto del útlimo riego) - También he hecho un riego foliar con un resto del ultimo riego foliar 20/03/19 - 1.000 ml con todos los nutrientes de la semana @ 0.9 E.C. PH5.9

Week 4 update. It has been very busy lately, so I have been able to spend less time on the plants than I wanted. Nutritions well maintained. It has now become a collection of different types of growth. 1 plant grows up very wide and high and is already in bloom. The other is more elevated and is now also in bloom. The third bumps a bit behind it and only has some signs of pre-flowering but no buds. The smallest does quite well for its size. My idea is to use 3 different techniques now. For the 2 largest a scrognet. The other, second largest, I am not finished yet. Maybe make cuttings? And the smallest, I want to have 1 big top. No idea if this is possible in 1 tent but it seems fun. I keep the light schedule on 6/18. They are doing very well.

Woche 8 und weiterhin sieht’s gut aus ein bisschen entlauben sonst nur alle 2 Tage gießen Die Gorilla Zkittelz struggelt weiterhin bildet aber unten wieder neue grüne Blätter

Day late on the update, pics/vids were taken on time tho just been a crazy week of harvests and trimming and keeping up with these. I linked up with Rain Science Grow Bags on Instagram and got them to offer all my followers and friends a discount of 10% off entire order from their site with the code ' bangdang ' so if anyone is in the market for a pot upgrade use that code. I got them in the mail 3 days after I ordered. Reason I went with Rain Science is because they offer identical air flow for rapid growth as the radicle bags, just using a different material and a tighter knit so water doesnt flood out the sides during feeds and when you pick these up when the coco is dry, it wont fly all over the tent like with the radicles. They're the optimal bag for autoflowers especially. These 2 are doing great should get interesting in the next few weeks. I actually ran 3 of these in 1 gallon pots a few months ago and 2 atleast came out pretty good so the bus shots are from the 2nd pheno of those.

Start of week 3 everything is looking good so far