Sono molto felice della mia pianta e dei suoi frutti spero in un buon raccolto da secca 😉 io direi di farmi fare altre 3 settimane di fioritura e poi raccogliere. Saluti 🙏🙏

Day46: still no big problems, ~20 days left Temperatures are a bit high, otherwise this strain would be more purple by now.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Well, this was a interesting first journey.... the buds finished drying today, 5 days drying, added pics and vids. Just curing to go.... ah nice buds :) Edit: i forgot to mention i went over the buds when i harvested them, and checked / removed any impurities, like gnats caught in the sticky bud.... picked em clean with tweezers... 👍 Edit: needed to air/dry out the buds abit more, and get some Boveda Humidipak for curing.... it's looking good... i'll update with some pics of trimmed, cured buds 😎 The buds turned out beautiful, pungent, pine, berry, citrusy, daytime, medical..... this has theoretical 1:1 - 21% THC, 19% CBD, so it's not going to give u a knockout high.... its medical grade 1:1 ....... side effects - happy, relaxed. haha :) I weighed it dry and came up with about 123.32g (4.35oz) ... i used abit of guesswork in the weight, not to concerned with weight, as to quality.... (that weight is with the test buds added in, +-15g.) .... you can guess the weight from the pics and vids, haha I really enjoyed growing this.... i found the website by looking up the seeds, decided to start a grow diary, it's my first grow, i made alot of mistakes and growdiaries really helped alot, joined a chat group with some other really good growers. Edit - Day 111 - added vid/pic, Been curing for a week, burp and roll em abit twice a day....... smell and taste nicer each day, 👍 Edit - Day 112 - added good vid of trimmed up test bud, beautiful ...... I hope You enjoyed the Diary..... Good Buds 😎

29.09.25 Blütetag 49 Noch 6 weitere Tage können die Damen noch Dünger ertragen, danach heißt es langsam flushe für 10-12 Tage. Folgendes Programm steht an: 29.09.25 Blütetag 49 kein Gießen 30.09.25 Bt50 GHF PK-BOOSTER + AN Sweet Bud 01.10.25 Bt51 Wasser je 1l 02.10.25 Bt52 AN Overdrive + Biobizz Topmax 03.10.25 Bt53 Trocken - Kein Gießen 04.10.25 Bt54 Bud Factor X 05.10.25 Overdrive + 0,25g/L GHF PK-BOOSTER Danach nur noch Wasser, Hesi Power Enzym, und Carboload von AN Voraussichliche ernte AppleFritter / PermaFunk am 15-19.10.25 Die LaBomba wird wahrscheinlich noch 20 weitere Tage brauchen bevor ich die flushe

Week #18 Gelato-K By Kannabia Week #18 Mar 8th-15th Week #7 Flower This week she is packing on weight buds a swelling trichomes are still mostly clear. Thank you for stopping by!! Stay Growing!!

Day 43 flowering today and these girls are stunning. The two smaller girls are covered in trichs and cloudy already !!. The bigger 2 could go on still . i am going to give them another week with a watchful eye i think. I have gone on to the Dragon force now so lets see what that can do !

Wao some.of them.just went crazy. Their name is showed so 3 of them I know. Those are the biggest ones FBT 31 - Stawberry Pie, FBT 32 - Lemon Pie and FBT 35 - Gorilla Cookies. They started growing crazily. LST is done. It is too hot inside some leaf burnt but I removed summer time outsode is 3p degrees so it raises up the temperature inside as well.

This week was exciting, my honey cream took off and now has a lot of side stems so I know I’m going to get it bushy beforehand flower! I also been training it so I can get the unders to come up and if you see from the photos I’m doing great! I’ve battled the heat now so I should be smooth sailing from now! I’ve also topped the side stems! (Last topping) I’m going 6 weeks with them! Look out for my other diary I’m uploading... Cherry bomb! It’s a week older than HC! Also have special kush to upload, they’re but slower but have now are showing grace! Also all my diaries will Ben correctly dated as I’m using the time stamps on my photos in there albums! Keeps me on track of my weeks!

So this week after I gave it under nutrients I apparently gave it too much nitrogen while having a potassium deficiency. Shiney dark leaves, So i fixed that, but some didn't bounce back, and I tried nitrogen. I think they are doing pretty good considering everything I've put them through SO FAR. lol. Nutrients are NPK Raw's total lineup, follow their instructions at first, Fastbuds adjustments as of this week.

Fast Buds - Wedding Glue Day 19 Closing in on start of week 3 Light - ViparSpectra XS2000 Watering every other day to every 2 days Slow growth in seedling stage but I am hopeful she will explode in vegetative stage! See you all next week!

Good week in the tent, finally now seeing the flowers kicking in. Only took about 3 weeks under 12/12, lol. They've stretched quite a bit since the flip, and by the looks of things should yield more than I initially thought. -10/6- Gave the girls a drink and a defo today, in the interest of airflow mainly. Don't want a repeat of last time. No matter how miniscule the amount of mold, any is a serious issue by my (and anyone resonable's) standards.

This week in the 5x5 had some good changes! Put on some good nodes and put on some resin!!! They all 3 pretty much look the same they have good node spacing and the stems are way sticky to the touch. Monday starting off week 5 gave them a top dress of craft blend 1/4 cup mixed into the top lil bit of soil and straw bed. Handwatering each plant gets 1/4 gallon at plant base and 1 gallon spread between plants. Everything going food look happy. Wendsday plants looks like they liked the Monday treatment so proceeding as normal. Gave them 1 top. per gallon of water along with 2 top of mollases to make the soil happy and some glacier rock dust a good plop. Friday everyone looked good maybe lil strong on nutes will only water as normal for the week. Sunday end of week 5 just straight water 1 plant got lil burned like I was thinking but everyone is happy happy happy. Vivosun fan I had for 4 months died had to replace with back up fan. Enjoy the pics

All right another week.... Ok my Gorilla girls are growing beautiful right on schedule did some LST as you can see to try and keep the canopy as even as possible and get the lower branches up even with the rest ..I also tied down the main branch to keep it even with the rest as it grows... I will be going into flowing probably Monday ..by then they will be pushed out and just the right size...... Not much else to say everything is dialed in perfect .. Until next week.... Smoke a fatty, help out your fellow grower.

420 Fastbuds FBT2307 Week 5 What up grow fam. Hope everyone's grow are going good. Weekly update for these girls. The 3 of them seem to be growing and progressing well. Not as fast as the others in the tent but over all seem OK. The bigger of the 3 is like the odd one out as it is giving me trouble since sprout being super temperamental. The other two are a bit shorter but overall seem healthy. I did do a molasses tea this past feeding so hopefully that'll feed the microbs in the soil allowing the plant to intake what nutes it needs from a healthy soil. Still spraying once a week of Pure Crop 1 to combat any pest,mold, and promote healthy leaf growth. All in all Happy Growing.

No issues this week.

Day 29: LST is done. I set the lamp approx 36 cm away. BlackBerry Kush, LSD-25 started flowering. All of them are OK started growing rapidly. 600W LED, 20 hours on 4 hours off the same with ventilation. Water intake: 75 ml per plant plus triggering, humidity around 60 percent. Fertilization is on Monday, Wednesday, and Friday with BioBizz family, Tuesday, Thursday, Saturday bat guano and Epsom salt. Day 30: I did LST again. I didn't have enough elastic band yesterday so I bought today and bent them better. I also set the lamp closer approx 34 cm. Day 31: I did LST yesterday and bent them. They already "lifted up" their heads toward the lamp. As they grow I will keep bending the main stalk and the side ones. I will try to create a spiral form and spread the plants with time. Day 32: Most of them started flowering except Colorado Cookies. BlackBerry Kush aggressively started floweing. This plant is very strong and aggressive. Day 35: pre flowers nicely coming along.... Balckbeery Kush and LSD-25 are the quickest. I changed the lamp as it is now really good.... so I placed an other 600W lamp there. I will swap these lamps one week here other week there. I will change that lamp after harvest. End of the week the tallest is BlackBerry Kush with approx 25 cm (I bent them so can't give perfect measurement) and the shortest plant is approx 18 cm.

Week 11 (03/28/22 - 04/03/22)

Week 7 for the banana purple punch from fastbuds 420!!! We gave the last week of xpert nutrients feeding and will continue now with only water, harvest time is soon and at the moment the buds are looking great, nice smell in the tent and the buds are nice and covered in trichomes! The plants are not that big but of course i have quite some plants together in the tent and 7 liter pots so they stayed a little smaller, next time i will do maybe less plants but with the bigger 420 fastbuds airpots!