visible flowering week 4 the bruce banner seems to be a late flower there doing well on there Plagron nutrients and there loving the new sun Spectrolight Blast 400

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

16/01/26 - Going well now all settled into their 20L pots of livingsoil. Will add the scrog in the next week

This is the 8th week. Thursday will be 9 weeks of 12/12. Some trichs are amber. They just added a good bunch of girth over the past few days, but most of the pistils are red now. The rest of them have been dropping leaves consistenly but the canopy remains full so they must be putting out more leaves. I just flushed to 80% runoff and found out that I had 1200ppm. I just got an ec meter but my last reading said closer to 600 so idk what happened there. I flushed until I had runoff ppm of about 150. Now they're back in the grow tent. I don't want to keep assaulting them with my microscope. I think they're ready. Just gonna let them settle another day or two to dry out a bit. I took one small branch off the small Wedding Cake plant on Thursday and I've just been watching it dry on my counter. It's been 5 days and the stem snaps but still leaves a fiber connecting. I put it into a small jar with a 64RH thing. I just want to try it. It already smells amazing. The leaves on the big CBD Blue Shark plant are going really yellow and some other colours are starting to come out as well. The buds all fattened up and got more frosty again. I was worried they were foxtailing but they got more calyxes underneath the peaks and have been making more of a pyramid shape. They're really close to peak. Harvest day will be March 17 or 18. March 17 1023pm chopped and hung after lights off all day. RH was 65% before I chopped and 50% after I closed the window and cut the plants off the saturated pots. I did a wet trim to prevent mold. The buds are sticky af. Gonna hang them to dry for 7-9 days before curing in jars :) March 18 : checked the RH when I woke up and it's chilling at 45% 😎 March 19 : just checked to see how they were drying. There are seeds in every plant. RIP I'll update when I put them in jars and again a few weeks later for final review. Overall it was a good grow despite the rocky start and a good introduction to the mainline technique and my new grow space. Next grow I should have everything pretty well dialed in. March 23 They were hanging for 3 days. Rh was around 45, then spiked to 65 overnight when I forgot to turn the fans back on. Temp was high at around 26.they felt wet after 2 days, and bone dry after 4 days. The stems still didn't snap but I put them in jars anyway. A day later, jar RH was 50%. I burped all the jars and there was a smell from the new jars. I took all the weed out, washed out the jars, and did a dry trim on the weed. One of the Wedding Cake plants must have gone hermie. I didn't see any bananas and didn't even realize there were seeds until the very end. The seed pods looked just like calyxes while the plants were growing. Many of the nugs are unusable. Under the sugar leaves are just layers and layers of seeds. Some weren't hit that badly. The CBD Blue Shark is much better off but still has some seeds. I'm trying to stay positive. It's a bit of a shock though. I'm anxious to try the finished product. If the flowers at least taste good and have a good effect, all is not lost.

Nuevamente repito siempre el mismo proceso en el cuidado de la planta en cada etapa. Siempre cuido el ph que para mí es lo más importante en este trabajo. Despúes todo lo demas se puede mejorar.

160 g Dry

Las flores cada vez mas olorosas, ya dejaron de estirarse y están engordando bastante, todo apunta a una abundante cosecha 😁... Abajo les cubri la tierra con bolsas de plastico para reducir la población de moscas, no son minadores de hojas sino mis plantas ya estarían muy mal con la cantidad de mosquitas que tengo. Ahora reduje los riegos a una vez por semana, porque como tienen 80 litros tienen bastante reserva de humedad. Los bichos bolita también se reprodujeron bastante, la próxima seguro subo vídeo o fotos de los bichos que tengo en las macetas.

Day 53 and the second candy kush is done

Glad its over. Wont grow her again. Finishes to late for Ontario. Last girl was harvested early due to snowfall. Taste is pretty good. Potency is lacking for me. She will make some good hash though.

Day 120 First Day of the week, 46 days of flowering. Day 123 49 Days of flower Trichomes look about ready of Plants B and D. Day 124 50 Days of flower, First day of Week 8 of flower Chopped plants B and D( had to chop in sections, because of the trellis net), hairs were mostly orange/brown, soil was dry, cut off most of the dead and dying fan leaves. Buds look beautiful. Hang drying at 65°F@50%RH. Plenty of airflow, but not blowing on the plants. Watered Plants A and C with 2 gallons of plain non ph'd water. PH shouldn't matter at this stage in the grow. Day 126 52 Days of flower Watered with 2 gal of spring water non ph'd.

Well well exciting times ahead my favourite time multiple buds sites appearing all over I'm so impressed with the new Mars hydro ts1000w the growth is amazing. I have to say this strain is the most smelliest I've grown very pungent cannot wait for the coming weeks to see these buds stacking some weight

week 9 and LOOK AT THAT !! 😄 Im in love with her perfume, the smell is amazing and she is growing by the biggest autos i have ever seen, unfortunatly as you guys can see im running out of space in the 5x5, but thats what it is, super confident i will have a huge yeild from this tent. see you later, give a like and comment, tks 😎

Day 63 - lookin good.... haha.... um, tanks stabilized, ph 5.8 - ppm 1200 .... she's feedin alot..... buds are fillin and lotsa white furries.... listed the nutes and amounts etc.., but i'm kinda feeding her as she needs.... so every few days she is drinking 2 lts +- and i'm just mixing up a neut solution in a 2 lt bottle every day or 2 ..... sometimes she just wants abit of water...... Day 64 +vid....shows her deep green and gloss... abit of crystalic :) Day 65 - vids pics.... trimmed her, and abit of bending.... Added blood and bone.... she's stretching alot, 60cm+ ........ Day 67 took all the early buds on all the stringy 0ff shoots.... Poor lighting vids, but don't want to mess with her flowering, :) 👍😎👊

Hello to everybody and welcome back for the weekly report. As of today we are completing the third week of vegetation and was definitely a good week. The plant reached 15 cm of height and is starting to look pretty decent! I love the vivid and nice green colors of this Titan F1, this make me think that I am treating the plant in the right way and is healthy. AT LEAST I HOPE. If we go a bit deeper in the rabbit hole actually this week couple of things went on. First at all I was away for almost a week so to compensate my absence I did water the plant before leaving...with ABUNDANCE, maybe even a little too much :) That much water that basically I didn't add anymore for the next 8 days, basically till today. However the plant didn't complain and keept growing strong. The lighting now is ALWAYS full power. 100%. 24/7 no stop. The temperature is always between 21 and 23, the perfect spot. Humidity is somewhere between 44% and 50% Today was the FIRST watering WITH nutrients from BioBizz. 1 liter of water, the vase didn't drain off. I think is good. If somebody is interested about the nutrients is all documented in my diary with ml, type and liters. Week by week. This was the first watering with nutrients, lets see what will happen in the next 3/4 days. I am really curious to see how the plant will react to the drugs :) Also something else very important is that in 2 or 3 days I will also apply some LST and bend the leaves outward. I want this plant to be kinda compact and that as much light as possible is directed to the flowers where eventuality it will be needed. I must be careful, I was reading a lot of Titan diaries and noticed that they had plenty of broken branches due to LST. I just hope to don't make damages or cause excessive stress. We will see how it will go. Next week probably I will do also some defoliation if the plant gets too bushy, dunno yet which leaves cut off but I can and will manage, uncle google is my friend :) Last picture are plants of chilli Ghost peppers, spicy stuff, they are growing together in harmony with the Titan F1. I hope eventually I can enjoy both of them cuz I love to get high and suffer from capsaicin :) :) Jokes a side, basically I am just fuckin around and looking what I can come up with. The last plant is a carnivorous plant, the one that closes the trap and basically eats alive the prey. Pretty cool stuff that's why has the honour to grow next to the green dope! I think that's all for this week and we see at the end of next week! Good luck to everybody!

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Have fun with the update. Hey everyone 😀. Another nice week goes by with a great development :-). Today it was placed in the flower chamber with a time interval of 12/12 hours. 1 g of GHSC enhancer was added per liter of water. The tent was completely cleaned and the humidifier was refilled. I wish you all the best 🙏🏻 You can buy this Strain at : https://www.zamnesia.com/de/3271-zamnesia-seeds-blue-dream-feminisiert.html Type: Blue Dream ☝️🏼 Genetics: Blueberry x Haze 20% Indica / 80% Sativa 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

week 9 and LOOK AT THAT !! 😄 Im in love with her perfume, the smell is amazing and she is growing by the biggest autos i have ever seen, unfortunatly as you guys can see im running out of space in the 5x5, but thats what it is, super confident i will have a huge yeild from this tent. see you later, give a like and comment, tks 😎

Eccoci qua all ultima settimana di vegetativa di questa bestia! Penso che le piante crescano davvero meglio con questi casi antispiralizzazione, prima pensavo fossero dicerie, ma sto sperimentando che le ragazze crescono molto di più rispetto rispetto alle sorelle nei vasi normali, questa ad esempio è germinata 10 giorno Circa dopo le sorelle, e sta rapidamente raggiungendo le stesse dimensioni! Anche la sour Apple di anesia ho dovuto spostarla e portarla in fioritura una settimana prima di queste, xke stava diventando troppo grande, ora è a 80 cm di altezza rendetevi conto! Al prossimo giro tutte le mie ragazze avranno un nuovo vestito, ovvero il vaso antispiralizzazione che permette alle radici di avere un accesso maggiore all ossigeno, e ci sono maggiori cicli asciutto/bagnato significa che la pianta respira e cresce meglio, raddoppia la velocità di fotosintesi secondo me! Merito anche dei nutrienti di Terra Aquatica naturalmente sono orgoglioso di fare parte della loro famiglia e utilizzare i loro nutrienti famosi ed orgoglio in tutto il mondo! Un buon 420 a tutti alla prossima settimana 💪😸

Week 2 for the solo Afghani went smooth. Looked a little slow to develop her first proper set of leaves, but the root development should help speed up her progression. Still feeding off the slow release 444 and is now regularly drinking about 2oz of water every other day. I also spritz the bottom of the outer cup to keep moisture levels high for stretching roots. She's about 36" from the light. Hoping to get a little stretch to make training easier. Added a couple new pics to get the week caught up. Stay tuned, week 3 starts training.

Some larger fan leaves especially mids got a little edge burn. I think because of a little too much dry back.