10/8: 2hours of wet trimming.

Powder: GREENHOUSE BIO FEEDING Line which are organic! For LIQUIDS ******GREEN BUZZ LIQUIDS***** also organic. Also i’m using their LIVING SOIL CULTURE in powder form! MARSHYDRO ⛺️ has large openings on the sides which is useful for mid section groom room work. 🤩 ☀️ MARSHYDRO FC 3000 LED 300W ☀️Also special thanks to VIPERSPECTRA P2000 (200W) & XS2000(240w) LED growlights 💨MARSHYDRO 6” in-line EXTRACTOR with speed-variation knob, comes complete with ducting and carbon filter.

4. Woche hat begonnen

This was a pretty explosive week for growth! These girls really took off this week! I have been topping off the res with just plain RO water this week after the change and for this run I have kept the strength of the nutrients pretty low and have been hanging around 0.6 EC, I don't see much of a need to increase at all. I am going to continue to keep a lower EC and just watch the plants. I think last run I may have fed a little hot a time or two so gonna go low and slow this run and it has been working out so far. I will be putting my trellis net up either today or tomorrow because I think I am really going to need it this run. I noticed yesterday that it looks like these girls are showing signs of entering the flowering phase so I need to get set up and be prepared for the stretch! I'm getting a little nervous about the height of the plants as well because they are growing fairly tall pretty quick, but hopefully the net will help a bit. No issues to report really and they look healthy as can be! Thanks for stopping by and checking out my grow! As always any tips, comments, recommendations are always valued and appreciated! Thanks everyone you guys rock!

Fucking chem og how resilient is she, for sure, she's a fighter, came back as determined as ever to pack it in some more, she didnt mind the dark, was a bit scared about the mold, so far no signs of it so let's all make a prayer for chem og, hope the taste is what i enjoy kinda fun flying blind ... but even a blind person would notice what an indica this is 😅 last week of smart proteins no nutrients added yet, just plain water and one point four bricks of shitting in my pants what a shake down my leg ! I'm smoking Dr. Grinspoon rn, and i've listened all i can to cypress hill's dr. green thumb and they say "I never grow at my home" but what they dont say is all about the rest of it lol abricots? peaches ? 🚀 dropped her down to 10/14 so she's got plenty of time for resin production now she's a stinker smells like citrus and indica, or more like indica citrus, does that make sense ? 👊

Day 57 this little lady last weekend showed up with some burning on the leaves but I’m. Not really sure it was an excess of nutrient, signals of a deficiency of K and P are also visible. So I just gave oh controlled water with regulator and enzym+ and some calmag, now everything looks fine and i ‘ m sure I t will be great this week. Tomorrow, feeding day! Day 59 lady si farting up every day, I’ve been feeding her the last two days. The yellow on the leaves is just because of a water deficiency from the last week, I wanted my lady to absorb everything I gave. On day 57 I gave 1 lt but it was too dry, so on day 58 I gave her another lt and she took it all with PH 6,2 and PPM 1000z I think I’ve got 20/25 day max left so this will be the last week of fertilising from Day 70 I’ll start mi flush. Day 62 today feeding day I finally get my p-boost and k-boost from aptus. She drunk 1 lt with ph 6,1 and ppm 1090 Let’s see🌱🌱🌱🔥🔥

Both girls are healthy and happy. Buds are solid although still a bit small. Today my Mars Hydro TSW2000 arrived and it's already unpacked and hung. I raised the light dustance to 45 cm. I also moved the cheapo 100w LED to 2 new FastBuds Zkittlz Auto seedlings. Wow, what a difference good lighting makes!! The Mars Hydro TSW2000 is well made, easy to install and bright-ass daylight LED!! I expect to see significant fattening of the buds over the next 2-3 weeks. Update: I swear the existing buds doubled in size within the last 24 hrs after switching out the light to the Mars Hydro. I'm excited to see how these 2 finish. 4/22 update: I'll be damned! The box from the new light (which is heavy, double wall corrugated) fell onto both plants...I heard snapping everywhere! Good ole electrical tape to the rescue!! These girls look wounded with all this black tape! It's several hours after bandaging and I see no wilting or noticable changes....keeping my fingers crossed! The videos show both plants pretty well recovered from the damage except the purple stems and veins. I have some cal/mag coming Monday.... hoping that will fix that issue. Both are loving this new light and continue to pack on the bud weight.

She is very close to harvest so fresh water flushing has started. If I had to describe her buds I would say they are very COARSE. The ovules are very large in diameter compared to any other strain I've grown. I left the tape measure visible in the video for scale. Good? Helpful?

So for the very first time I trim a lot of green away- while this I got a scrimp while every single leave that I remove. Idk maybe I should remove some more the plant already recovered be me not haha All baby are at flower now but I f..ed up and brocke the main while lst…it’s my first grow so I take it easy and will see what will be at harvest day. Think about to take another tent looks to small for they’ll all. Didn’t thought they get this big

Super gewachsen tolle Woche bei der einen Dame habe ich das FIM verfahren angewendet. mal schauen. Die andere bekommt noch ein anderes HST lasst euch überraschen. ;-)

Dia 126, 5a semana de floración se ven muy sanas y estan empezando a formarse los cogollos, la floración va un poco lenta, se puede ver la 1051 y la Chocolate Wafflez ya estan llenas de resina , la Glow Starz y la White Noise siguen generando cogollo, hemos visto algunas orugas que nos han sorprendido ya que no han habido lluvias todavia y las plantas estaban protegidas, pero supongo que las bajas temperaturas generan las orugas

I’m happy one of the white widows is further ahead in flowering. I’m looking forward to enjoying some smoke.

This is a strain a costom breeder and friend of mine has asked me to try. I know little about the genetics other than the name: "Gorilla Glue Pineapple Cheese" Popped the seed as a back up just in case one of my Silver Haze cuttings didn't make it but they all did. This means along with the Gelato there will be 5 plants in this run in the 4x4 tent. I usually only grow 4, so might transfer this into a 3 gallon pot instead of a 5 gallon like the others, as in the past 5 plants in a 4x4 is pushing it for space. We'll see.

Plant is still tiny, unless something miraculous happens yield is going to very low!

We have reached the first harvest, I do not mark it as harvested because the other one is still going and will be harvested in 7 - 10 days. Here we are with our Blue Cheese F1 Hybrid by Zamnesia, it was the first to be harvested in just 59 days, born in fact on September 1st, all of it was harvested on October 30th, not bad at all. The flowers are really beautiful, excellent resin, skunky scent as we like it a little gray, as soon as we smell skunk we fly into the past, far from indie and commercial music and with generic nostalgic references. Here with a smell of skunk we teleport to the coffee shops of Amsterdam in the late 90s. It often happens to me when I smell the flavors and smells of the first super herbs I smoked. With Skunk and derivatives more than anything. And then with Cheese which is precisely a derivative and with Bubblegum I go straight back to that coffee shop. The amount of grass is average, the plant is not very tall, about 90 cm and has been cleaned underneath, the largest is 100 cm, it will give us a lot of grass, the phenotypes are slightly different despite being f1 but at least two phenotypes seem obvious to me, we must grant them, mom and dad. I did a big flush but the plant arrived a bit green anyway, I had exaggerated in pumping a bit and I anticipated the harvest by about a week when I saw my jars almost empty. Let's be clear, we are still at an excellent amount of mature pistils, about 75%, and as for trichomes we are a bit amber, mostly cloudy and also some transparent still. But whatever, we are ready anyway. We really liked the easy to clean harvest with those flowers that come out well without leaves that are too difficult to remove. // Strain Description // If you were looking for a reliable, versatile and accessible strain, here it is. Blue Cheese F1 Automatic by Zamnesia Seeds uses F1 genetics to produce uniform, rewarding and easy-to-grow plants. This strain stays small, so you will have no problem finding a place to grow it. After just 10 weeks from seed, you can enjoy tasty buds with relaxing effects. - Strain — Get a seed of this fantastic strain - https://www.zamnesia.io/it/10673-zamnesia-seeds-blue-cheese-f1-automatic.html -l - Fully organic soil and fertilizers --- https://plagron.com buy at www.zamnesia.io - Growbox and ventilation system --- https://www.secretjardin.com/ - Light — P2000 - https://www.viparspectra.com - Music and sound --- I made my girls listen to 432hz frequencies and black music from www.radionula.com - Z --- You can find these seeds, much more from the world of cannabis, mushrooms and an incredible series of accessories and gadgets on the reference site not only mine but of many growers ---- https://www.zamnesia.com

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Day 57 (2020-07-05): Feeding @ 5.8 PH. Removed a few leaves. Day 58 (2020-07-06): Noticing a few amber trichromes on the CBD Crack. Wondering if it might be time to start thinking about pre-harvest flushing. Day 59 (2020-07-07): Fed today @ 5.75 PH. Day 60 (2020-07-08): No news today. Plants are kind of on autopilot at this point. Day 61 (2020-07-09): Waiting to feed until tomorrow. Day 62 (2020-07-10): Fed today @ 5.6 PH Day 63 (2020-07-11): Leaves on both plants look pretty rough at this stage, but buds are looking nice all around! Guessing 1-2 weeks left on the CBD. Will probably start flushing sometime next week. G14 seems to be running behind at least a week.

Blue Sunset Sherbert ❤️ – What a beauty this one turned out to be! The buds have absolutely exploded in size and density! 💣💜 The terpene profile is something truly special – rich, unique, and completely unforgettable. The plants have taken on stunning dark hues, with anthocyanins beautifully developed, giving them that deep, almost mystical color. Frosty, dense, and shining like gems – they’re almost ready to meet the scissors ✂️✨ I think by the end of this week it’s time for harvest, and I honestly can’t wait to taste the final result. Stay tuned – this one is a masterpiece in the making! --- Blue Sunset Sherbert ❤️ – was für eine Schönheit diese Lady geworden ist! Die Blüten sind regelrecht explodiert! 💣💜 Und das Terpenprofil? Etwas ganz Besonderes – so einzigartig, so intensiv, einfach unvergesslich. Die Pflanzen haben sich wunderschön dunkel verfärbt, das Anthocyan hat sich super gebildet und überzieht alles mit einem frostigen Look. Hart, dicht und einfach nur beeindruckend – ich denke, Ende der Woche ist Erntezeit ✂️✨ Ich bin unglaublich gespannt, wie sie schmecken wird. Bleibt dran – das hier ist echtes Headstash-Material!