2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

For LIQUIDS & NUTES ******GREEN BUZZ NUTRIENTS***** organic. Also i’m using their LIVING SOIL CULTURE in powder form! MARSHYDRO ⛺️ has large openings on the sides which is useful for mid section groom room work. 🤩 ☀️ MARSHYDRO FC 3000 LED 300W 💨MARSHYDRO 6” in-line EXTRACTOR with speed-variation knob, comes complete with ducting and carbon filter.

Flower is in its last week might push another half week out but i already stated flush at the beginning of the week as you can see the chlorophyll beginning to flush out ☃️😋🎄

Bueno familia ya finalizamos, estas red hot cookies son una cepa bestial. La verdad que el secado muy bien 7 días en Malla y a los botes, 45% humedad y 23 grados es la temperatura ambiental que han tenido en el secado. 4 plantas que se cultivaron en un armario de 1.20 pero estos ejemplares solo ocuparon 80x80. Así que ya está cultivada esta gran variedad que es novedad este 2020, americana de la familia Cookies, la Tropicanna Cookies (Girl Scout Cookies x Tangie) con un porcentaje de thc entre los 17/25%, una bomba. Que decir de Agrobeta, lo peta 💪 en cuanto a nutrientes. Un saludo y buenos humos.

💩Holy Crap Growmies We Are Back💩 Code Name FBT2309 Well growmies we are at 28 days in and everything is going great 👌 👉 So the low stress training👈 has been going great she's gonna be a big girl, shes in full flowering mode so let the stretch begin 👌 Lights being readjusted and chart updated .........👍rain water to be used entire growth👈 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

Pflanze ist inzwischen 1,70 groß und langsam wird’s eng aufm Balkon. Ebenso wird es langsam zeitlich knapp. Brauche dringend Hilfe Pflanze ist immer noch nicht in der Blüte obwohl wir schon Mitte august haben. Was denkt ihr abends früher reinstellen? Um die Blütephase einzuleiten? Letztes Jahr ging mein Experiment wesentlich früher in der Blüte ca Ende Juli, dieser unbekannte strain scheint länger zu brauchen.

Do-Si-dos auto is doing great. She was lollipopped today, and a selective defoliation In the canopy. She is stretching and developing more bud sites as well. Hopefully the stress doesn't bother her. She is ready to flower now. Nothing more to report. Thank you Medic Grow, Athena, and Zamnesia seeds. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

After flushing multiple times, and checking the ppm of the removed water, I realized how much nutrient build up I was subjecting the plant to. Big lesson learned that I need to add ph’d water without nutrients more often and check the ppm after I mix it back in. Leaves are starting to lighten up from dark green and I noticed very slight deficiency on one or two leaves. Now I’m at 25% nutrient strength and it is starting to grow noticeably faster.

Всем привет! Вот и прошла ещё одна неделя. Растение растёт и развивается. Шишки наливаются. Стоит очень интресный аромат, с фруктовыми нотами.

Plants are growing well under LED light... Hope to scrog correctly 😏

Hi everyone 👊🏻 thanks for checking in 😏, so we have started flowering this week on the bigger tent using the 2 Lumii 720w LEDs, we might or might not run into some hight issues well see… And we have the other 1.2 tent set up for the clones there under 1 Lumii 720w led delivered until I can another one sorted few weeks 🤙🏻 get them under that for a few weeks then get them flowering as well. Keep eyes open for progress 👌🏻

And there ready along with there big sister 67 days in flower to achieve the best smell I’ve ever smelt, these plants weren’t touched at all, not one leaf was removed throughout the entire grow, no nutrients, no PH the water, and I think it just shows for its self. same plan for drying and curing as the big one, will be back with a smoke review. Thanks for stopping by 👊🏻

Schönes Wetter, warme Temperaturen und noch nicht so kalte Nächte... kein schimmel ,Blüten wachsen und haben gut trichome gebildet. Habe bei der critical supercropping betrieben, um zu vergleichen, sie hat sich erholt und die buds haben sich an den geknickten Zweigen ausgerichtet. Es läuft nach wie vor ziemlich gut. Bis nächste Woche ✌️

So far this week I am starting to germinate 4 seeds. 2 are blue dream matic and the other 2 are pineapple Express auto from Fast Bud genetics. I also did a leak test on my diy undercurrent rdwc set up. I should have seedlings by the end of the week. Update 1. Both blue dream have 1/2inch tap roots and 1 pineapple Express has a 1/4 inch tap root. The other one has just opened up. Hopefully that doesnt mean they are going to be slow growers. Update 2. Sorry I dont have any more pictures this week. I dropped my phone and now my camera doesn't work. The 2 blue dream matic are about an inch tall and in rock wool. The 2 pineapple Express auto have not sprouted yet. The tap roots are slowly getting longer. I think it may be the temp of the seed starter. I purchased a seedling mat and have them on it. So hopefully I will have 2 more sprouts. I also have been fighting with fixing the leaks in the buckets. So I bought some flex seal spray and it worked!

Just as expected stated in our last weeks update this week is awesome! First signs of the flowers are showing and the plants look very beautiful and green. These plants are as good as it gets really loving these, the faint smell that they spread in the tent is very smooth and gives me a happy smile on my face every time i open the tent to give my lovely ladies some attention and nutrients. Next week i will make sure to have a picture of the developing flower. Talk next week. Cheers, Nibameca

she grows healthy outdoor ... the flowering begins for her .. we will see the process hopefully it will be a good...