2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Finally decided to get the new tent and it arrived a day after! Meanwhile the ladies moved into my closet for a day, because some top buds and leaves were already getting damaged. Refilling almost 5 L daily! Lets see how they grow with a bit more space to spread out :)

Watering when the soil is dry

The start of week 9 is going well. On day 5 and 6 of week 9 the temperatures outdoors we're in the 90s my extractor fan ran continuously and my grow room temps are hitting 83 to 85 warmer than I would like. It is day 7 of week 9 and the plants did well on vacation the 10 gal reservoir lasted with an extra 2-3 gal left when I returned. I did however notice a Nitrogen deficiency on the foliage(yellowing on lower parts of plant). I believe this is due to to the long time in between feedings. I applied my weekly Nutes. I will watch for improvement in the Nitrogen deficiency.

Week 15 for Gelato 41 by seedstockers She got a MASSIVE defoliation... session done to her. She looks a little naked as of now but she will bounce back nicely by next week😇 She really needed it, it was so bushy before you couldn't even put your arm into the plant to figure out what was what😂 Oddly enough 30% of the plant seems to be in pre-flower when the rest doesn't... She does get shaded the quickest in the garden but wouldn't expect her to be flowering already... Still hasn't been watered since the rain last week but i may have to give water once this week as we have 40+ degrees incoming 😰

She finally stopped her heightgrowth. Happy with that, because i don't have more space to hang the growlight any higher. I decided to give her more nutrients this week. She also did trink more water (4.2L in this week), although she was only exposed to the growlight and had no direct sun anymore. She smells incredibly sweet. I quite enjoy it, and sniff here every day 😅 Really curious how long it will take until i'm going to harvest her. I decided to wait until she has 60 to 80% brown trichomes. One of her leafes on the bottom still isn't looking too good and is more yellow, so i probably going to cut it in the next few days.

It's a snow storm going on in this tent! Just luv how frosty the buds are getting 😀

She is going all out right now. Nothing slows her down. I did a heavy defoliation and she just kept going balls to the wall. The silica blast has really toughened her up.

I didn’t take pictures before defoliation but these pics are from after the fact So you can only imagine. So many bud sites she should be stacked!!! The game plan is to move her into a 5 gallon once I get a chance this week. Glacial, 2-8-4 and some kelp meal will be used.

Ze heeft de buds best wel volume gegeven, ze ziet er weer gelukkig uit. Het heeft geholpen de bodem te bedekken met zand want ik zie geen vliegjes meer uit de grond kruipen. Laten we kijken hoe ze zich deze week ontwikkeld. Ik zal gedurende de week nog verse foto's updaten.

Flowering has started.

Stiamo ancora raccogliendo,ancora nessuna pianta é morta,sto rimuovendo piano piano le cime più mature,così facendo evitiamo le cime popcorn e diamo il tempo alla pianta di riempire anche le cime inferiori,per adesso levando quella fumata e venduta siamo all'incirca sui 780g,però aspettiamo anche il nuovo stendino,alla fine del raccolto dovremmo arrivare a quasi 2 Kili con 7 piante e 6 vasi,con una media di 333g a pianta,ho pubblicato tutto quello che potevo,ogni procedimento,spero vi piaccia e mi seguiate

Eccoci qui... Che splendore questa genetica, NON SEBRA MARIJUANA!!! Questa settimana posso solo dire che sembrano fatte di marmo. STRAIN 1 super compatta chissà come sarà alla fine del raccolto!!!! Si inizia a vedere il viola nelle foglie FINALMENTE!!!!!! Grazie a @Khalifa_Genetics per la collab e a tutti per il supporto🔥🌲❤️

Привет друзья. Моей растихе сегодня 112 дней 20.10 перевёл свет в режим 12/12 Начал применять LST технику на 19 дне и продалжаю применять её через день, а 18 августа добавил ДЕФОЛИЗАЦИЮ С 20.08 ДЕФОЛИЗАЦИЮ делаю каждые 3,4 дня С 20.08 LST технику делаю каждые 4.6 дней На сегодняшний день влажность 54% 5.09.2023 заметил высокий Ph 7.9 С 48 дня Ph не ниже 5.8 На сегодняшний день Ph 6.0 Начал кормить с 60 дня Canna Terra Vega PPM 870 Всем мира и добра! Не забудь поставить лайк❤️, если понравилась как прошла неделя И читайте наш TELEGRAM: https://t.me/smail_seeds #Smail_Seeds 😀

Had a bit of strip not much to say about this week again apart from flowering is happening finally and it's getting there with small bud sites popping out everywhere

23/4 : Received the Migro Aray 240W so i switched the lamps in the box. I'm not using the old 600w LED fixture. It will grow even better with the new light !!! 👽 Still don't have a basic grow nutrient, will try to grab one soon. 2 Pink Gasoline and 1 Capri Zun didn't make it unfortunaltely 😞 25/4 : The Migro light delivers the first results !! Also i ordered the whole FloraFlex nutrient line. Can't wait to receive these 😊

Week 18 december - 24 december 17 december - rega

d.50 a few spots appeared on the leafs. gave a 10 ml dose of bio p k and waiting a week before giving a dose of orgatrex and bactrex edit: d.50 i also gave 0.5 ml of calmag from Biobizz edit d.53 will i be giving her the orgatrex and bactrex treatment ✌️🌞 d.53 she got 20 ml orgatrex, 1g of bactrex and 0.5 ml of biobizz calmag d.54 added scrog and did lst with clips to even out the height and space… still need to adjust it.

Gracias al equipo de Kannabia Marshydro XpertNutrients y Trolmaster sin ellos esto no sería posible. 💐🍁 Amnesi K - Lemon: La Amnesi-K Lemon es una semilla formada a partir de un trío de reyes, la Amnesia, la Lemon Skunk y la Jack Herer. Este matrimonio de tres dio lugar a una gran sativa que, con tonos dulces, terrosos y muy cítricos 🌻🚀 Consigue aqui tus semillas: https://www.kannabia.com/es/semillas-marihuana-feminizadas/amnesi-k-lemon 💡TS-3000 + TS-1000: se usaran dos de las lámparas de la serie TS de Marshydro, para cubrir todas las necesidades de las plantas durante el ciclo de cultivo, uso las dos lámparas en floracion para llegar a toda la carpa de 1.50 x 1.50 x 1.80. https://marshydro.eu/products/mars-hydro-ts-3000-led-grow-light/ 🏠 : Marshydro 1.50 x 1.50 x 1.80, carpa 100% estanca con ventanas laterales para llegar a todos los lugares durante el grow https://marshydro.eu/products/diy-150x150x200cm-grow-tent-kit 🌬️💨 Marshydro 6inch + filtro carbon para evitar olores indeseables. https://marshydro.eu/products/ifresh-smart-6inch-filter-kits/ 💻 Trolmaster Tent-X TCS-1 como controlador de luz, optimiza tu cultivo con la última tecnología del mercado, desde donde puedes controlar todos los parametros. https://www.trolmaster.com/Products/Details/TCS-1 🍣🍦🌴 Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes líquidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ 📆 Semana 2: Fantástica semana, estan creciendo de una forma espectacular gracias a la ayuda de @marshydro, @xpertnutrients y @trolmaster. Se puede apreciar un tercer nudo aunque la aplicación de cola de caballo y el estar en una maceta pequeña ha influido en el tamaño de ella, se recupera muy rápido 😁. Esta semana espero trasplantar a la maceta definitiva. Potencia de la lámpara: 50%