MI MEJOR COSECHA HASTA AHORA, 425 GRAMOS EN SECO DE FLORES PERFECTAMENTE MANICURADOS, DUREZA DE COGOLLOS, INCREIBLE EFECTO Y SABOR, INCREIBLE PRODUCCION, GRACIAS SENSI SEEDS !!!!!

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Básicamente lo mismo, aunque está semana pilló bastante más horas de luz(tuve que irme fuera de casa dos findes seguidos, en los cuales la dejé con la luz encendida full 24/7 en la caja) Como experiencia utilicé café reposado 48h para "alimentarla" e intentar bajar el Ph de la tierra (coco ph6+hummus ph7+vermiculita ph8= ph total 7) El otro experimento fue cubrir la tierra con coco para que el tallo eche raíces y aprovechar su espigamento! Las horas de luz solía dejarla 8h en bombilla mientras duermo, y el día la pongo en la ventana a que tome el fresco, pero apenas tiene luz directa, me da la sensación que crece más rapido cuando vuelvo del finde, estoy replanteandome tenerla más tiempo en la caja con luz, porque la veo aún pequeña, y quizá ponerle un ventilador de pc o algo🤔..veremos, aún tengo el papel de aluminio al lado de la caja y ni se lo he puesto, con eso os lo digo todo☝️🎃

good afternoon growers we are at day 26/07/2024 one day later of the week 2 of life of the seedlings, today we will continue watering to amount of 150ml/l per plant, a total of 2l of osmosis water with a PH of 5.9 and an EC of 0.5, with a total of 8 ml of fertilizer 3ml of deeper underground, 3ml growth boom, 2ml enzym boom, in the next irrigation of the following week we will apply silicon to improve the cell walls and absorption of photosynthesis. buenas tardes growers estamos a dia 26/07/2024 un dia mas tarde de la semana 2 de vida de las plantitas, hoy seguiremos regando a cantidad de 150ml/l por planta , un total de 2l de agua de osmosis con un PH de 5.9 y un EC de 0.5, con un total de abonado de 8 ml de fertilizantes 3ml de deeper underground, 3ml crecimiento boom, 2ml enzym boom, en el siguiente riego de la sieguiente semana le aplicaremos al riego silicio para mejorar las paredes celulares y absorcion de fotosintesis

One of the plants has a slight pie dough smell, but still very minimal smell when I open the tent. EDIT: the smell is actually making it passed my carbon filter, and I am not sure why it is only 1 of the 2 plants giving off the heavy smell as well. The chunkier/darker colour one is the one that smells real heavy, smells really creamy/doughy. UV/IR has been on for the past few weeks, and will stay on until the end. The plants have gone through a bunch of issues so far, and are still going strong. Gave a tablespoon of power bloom to each, on day 31.

Week 2 is cruising along, and the lady is looking strong and healthy 💪🏼. Feeding her the full Canna suite with the pH locked at 5.8 and watering every 3-4 days. Everything's on track with no major updates—next week, I'll kick off LST!"

2/25/25 these plants are exploding with growth. all dark green and super happy. will be giving them another week and then switch them to 12/12 2/27/25 these plants are absolutely exploding with growth right 2/28/25 defoliating and tucking leaves. theyre all so lush and bushy. these genetics are surprising me after this transplant. theyre absolutely loving life right 3/2/25 these plants are growing visibly each day. i switched them to 12/12 today even though theyre only in the veg tent. after a couple more weeks, ill move them into the flower tent. so 2-3 weeks under a small 150 watt light, and then move them under a 300 watt light to finish

I had to change the light this week. To smaller lights because of some fan leaves was turn at the tips away from the lights

Hey guys :-) The lady has developed very nicely this week 👍. Since there will be space in the flowering tent for the next 10 days and it will then be switched over, I will no longer use any training. I'm looking forward to when the bloom starts :-) Otherwise everything was cleaned and checked. Have fun and stay healthy 💚🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : https://www.amsterdamgenetics.com/product/kosher-tangie-kush/ Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.4 MadeInGermany

-Strain: Purple Lemonade - Fast Buds -Tent: 5x5 Gorilla Grow Tent -Lights: Budget LED Grow Lights 2 x 250 Watt LED Full Spec/Red Spec mixed boards -Light Cycle: 18/6 -Soil: Fox Farm -Air Circulation: AC Infinity Cloudline T4 Inline Duct Fan WECLOME BACK GROWMIES! Week 8 here with our PURPLE LEMONADE by Fast Buds! January 14, 2020 (DAY 52) - Hey there growmies sorry for the one and only update this week, i got sick and just cant do it. i hope you all understand. 01/14/2020

All started to flower and the stretch begins! Lost a few tops with training. I will opt for just topping instead of LST (too time consuming) Will start slowly dropping nitrogen and increase phosphorous and potassium with some molasses! Last two shots are of last bit of LST and leaf tucking/defoliating

This week the new growth of the plants transitioned from looking relatively healthy to droopy and yellowed. I wasn't sure what the issue was at first and thought it might be light stress. After more research, I determined that I had what looked very much like a zinc deficiency, which is almost always a symptom of over-watering, which is the most common mistake for new growers, i.e. "Me". I had assumed that adding a good amount of perlite to my soil and using smart pots would prevent me from running in to any drainage issues but I guess not. I was up to 1.5 liters per plant when I realized that it was too much. I also realized I was misreading my syringe which I use to mix nutrients, and that i had been giving only 1/10 of the liquid nutrients that I thought. I'm super embarrassed about the mistake but I'm going for full transparency here, so there it is. Course correction takes places after day 31.

Horrible week :( 1 of the dosi's hermed so im down to 4 plants, 1 DNA Genetics Bakers Delight and 3 Barney's farm Dos-si-dos 33. ( I started with 6 dosi's -_- ) never running these again. The remainers are stacking up nicely after PK week. The DNA genetics bakers delight is absolutely gorgious, the smell is insane!! I'm happy i replaced my carbonfilter a couple of weeks ago. Def be running this strain again. Rock on Growmies

Windy rainy week. Another week or 2 and I think these girls will be coming down. They have had some rough weather and I don't think they will take much more rain. Buds are nice and hard so it won't be to much of a loss.

Chopped Chocolate Mint OG on day 61. It's been a pleasure to grow and looked like it was going to be my biggest yield. This was definitely the bushiest plant in the tent. She was very photogenic toward the end. The stem rubs smelled like burning tires and dill pickles, but when the buds came in they were surprisingly sweet. The smell tickles some weird nostalgia, like a candy you had growing up but can't quite remember. It's like a sweet grape tea, but with real grape instead of candy grape. It is maybe reminiscent of grandma perfume - ambiguously sweet, fruity, and floral. After the dry and trim, this came in at just over 40g. I definitely smoked a bunch before weighing, plus this plant had 2 sample cuts taken before harvest, so maybe actual weight would have been 7-14g higher than the actual result. Overall this plant was phenomenal and I would gladly run it again in the future.

Strain: Fast Buds – Mixed Photoperiod Pack #1 Grow Type: Indoor Pot Size: 3 Gallon Medium: Peat Moss & Perlite Nutrient Line: Athena Week 5 Update Man, I love this plant’s structure it’s a perfect 6-main manifold. She’s doing amazing so far and taking nutrients with no signs of deficiencies or stress. Everything looks balanced, and she’s thriving under her current setup. I’ll be flipping her to flower this week, and I’m really hoping she puts on a great show in the next phase. Feeding 1.7 L Athena nutrient mix (will increase gradually if needed). Indoor Climate Conditions Temperature: 18–23°C Humidity: 60–65% VPD: ~0.5–0.8 kPa See y’all next week! And don’t forget to use my discount code ISLANDTir STLVDXW for 15% off your next order at Fast Buds!

Doing nicely quite pungent, the buds are bulking up and some of the pistols are turning amber she's starting to mature !!!!

Phone was out of action , will try and fill up the missing weeks , 3 plants have been harvested All photos taken day 76

Week 18 Day 120 (11/12/2020): I checked the girls’ trichomes and they are looking great. They are definitely getting more amber but still a small percentage. Also most of the pistils have browned now so I think they are about 2 weeks away from harvest now. At the next watering I will definitely start the flush! Day 121 (12/12/2020): The girls are looking good today but definitely starting to go more and more yellow. But I think during this time it is actually good news and I will be happy for those girls taking up all the nutrients left into the buds to make them extra tasty!🤤 Day 122 (13/12/2020)💧: Sooo this week is the start of the flush, just 1.5L of water. They are definitely ready for the flush and the buds are looking gorgeous! So gorgeous, in fact, that we cannot wait for the girls to be ready. So I cut some stems off to dry 🙊 We basically realised that they will not be ready and dry by Christmas (plus we would want to still cure them for min 2 weeks) and so I cut some of the smaller stems off to get some buds ready for Christmas celebrations 🎄 I only cut off 2 stems (one from each girl) of the buds that were at the bottom. I’ve put them into a box with holes in a room that has good airflow. I’ll also make sure to weigh them and add it to the final dry weight. They do look super amazing though and definitely quite purple Day 123 (14/12/2020): Didn’t have time today to take pics of the girls but I’m sure they are fine 😉 Day 124 (15/12/2020): I swear the buds look fatter and fatter every single day that I check them. Looking great, smelling great, and will definitely taste great! Day 125 (16/12/2020)💧: Second flush of the week, great stuff. Just gave them water and ripped off some dry leaves. There are some stems at the bottom that are still quite weak and cannot hold their weight. At this point it is too late to cut them off but I have definitely learned to lollipop for the future! Day 126 (17/12/2020): What a great end to the week and just 1 more week to go! Buds are looking astonishing at this point. And I swear they are getting more and more red even though I thought they wouldn’t. The trichomes are developing beautifully as well, definitely harvest-ready in just a few days! And next week, I will be watering twice and probably harvest after the whole week is finished, so on Christmas day! What a great present 🎁