Psilocybe Cubensis Ecuador [2019]

I. Substrate preparation [8 days] 1) I sorted out oats (1.5 kg) and wheat (0.9 kg). It took about 6 hours to clean the grain from trash. Weighed cereals to calculate the antibiotic during decoction. I thought about numerology and the number 13 😂 I realized that January 13 should be a good date to start actions. 2) 12.01 Soaked cereals in boiling water from a kettle. A couple of years ago I soaked it in cold tap water, as some schroomers recommend on thematic forums, but overnight the cereals began to sprout. Since then, I have been pouring boiling water so it does not happen. In my opinion, this does not affect the adaptability of contaminants to subsequent thermal processing. The cereals were in the water for a day (t = 17C - 19C). 3) 13.01 Boiled cereals with the addition of an antibiotic: oats within 20 minutes after boiling with 750 mg of chloramphenicol, wheat 15 minutes after boiling with 500 mg of chloramphenicol. The antibiotic was simply crumbled with two tablespoons (at the rate of 500 mg per 1 kg of dry cereal), poured into pots and stirred for several minutes. 4) I washed the cereals under cold water in a colander (in parts, long and dreary), until the running water drained clear. Drying on a towel for about 2 hours. I made a “test with a napkin” (this is when you put the cereals on a napkin, then shake them off and look at the residual moisture), a small stain of moisture remained, but I decided not to dry further and add vermiculite to my substrate. 5) Mixed cereals, adding vermiculite (about 1 l) and chalk (100 g). The result was approximately 9 liters of substrate. It turned out much more than I expected :) 6) Distributed the substrate into one-liter jars: 1 cm of perlite on the bottom (I put vermiculite in some of the jars, because my perlite was unexpectedly finished halfway through), about 600 ml of the substrate, sterile cotton wool in the holes of caps, covered with foil and secured with an elastic band . It turned out to be 15 cans, filled just above the middle. 7) 13.01-19.01 I did 3-fold fractional pasteurization (that is actually tyndallization). I used nano technology in the form of a large pan and 3 flat stones from the river :) My pan fits 7 cans at one time, so I divided my cans into 2 batches, and decided to pasteurize one can in an airfryer (1.5 hours at t = 110C). I left cans in a closed saucepan until morning. Then they stood at the balcony for a day (t = 16C - 18C), while in the evening I did pasteurization of the second batch of cans. Thus, two days passed between each pasteurization procedure. II. Inoculation [3 days] 1) 17.01 Boiled water in a pan. Without removing the cap, rinsed the syringe (10 ml) with boiling water, took 9 ml of boiling water, put cotton wool soaked in ethanol under the cap, put the syringe into a zip lock, put it in an upright position in a glass. Thus, I prepared 4 syringes with boiled water for suspension. 2) 18.01 I made a spore suspension. I used prints from my previous cycles. Left spores for rehydration for 2 days (t = 24C). 3) 20.01 I took 1 ml of gentamicin sulfate into each syringe, shaken it so that the suspension solution was mixed with the antibiotic. An hour later, I changed the cotton wool in the caps that had become damp after pasteurization and inoculated jars with a spore suspension. 2-3 ml of suspension was poured along the wall into each jar. I put the cans in boxes on a cabinet (t = 26C - 27C).