Deepforest Super auto is doing okay. She is super hungry and in need of a solution change. I will be doing that in the next few days when I have time. Everything is looking good considering the fight with rising ph. Which is the hunger. Thank you Doctor's Choice, Spider Farmer, and Athena nutrition. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g.

*Week 1 Seedling/Veg - 09/25* So far so good, Mimosa one had a little slip with her first set but has since recovered accordingly and is filling out nicely. High Temp 82-85 - High Humidity 90 - 94% *Week 1 - Veg 09/28 update* 2 of the 3 seeds failed due to user error - currently only running girl, she is coming along nicely. Transplanted into a small container using coco. * Week 1 (Early Veg) update - 09/30* Thus far all is moving accordingly - Added some light myco to the coco and running in prop dome at 91% humidity and 81 degrees in temps. Light watering until the final transplant

So strong strain, so many flowers)

Que pasa familia, vamos con la primera semana de crecimiento de estas Gorilla Zkittlez Auto de FastBuds. Tiene muy buenas reseñas y pues me animé a colocar 5 plantas. Las 5 semillas una vez germinadas son plantadas directamente en macetas de 7 litros y salieron las 5, 100% de éxito en el ratio. La tierra que utilizamos que está en la publicación anterior es top croo al mix, aparte alimentamos nuestras plantas con Agrobeta. Por supuesto el ph se mide en cada riego y se mantiene en 6.2, regando cada 48 horas e intentando mantener la humedad un poco alta al principio. Las próximas semanas vamos viendo cómo avanzan. Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨

Week 12+13 Update – Final Harvest & Reflections 🌱✂️ First off, sorry for the late update—super busy at the moment! This will likely be my last report for now. I’ll still share the final results, but after that, I’m taking a break. BioBizz#1 – BT59 Harvested, and as expected, a disappointing yield—typical for BioBizz. Final weight: 71.88g AN#4 – BT65 As expected, the biggest plant in the grow! This grow confirmed that I’d stick with Advanced Nutrients in the future. Final weight: 163.88g AN#3 – BT64 This grow was an experiment in 60x60x120cm, and let’s just say—not recommended! Zero height control, only 2cm clearance from the LED. Somehow, she survived. Now I’m super curious about the final yield! Final Thoughts Advanced Nutrients proved superior, and BioBizz didn’t impress me. Stay tuned for the final numbers from AN#3! 😏

They still seem to be growing, although going in the room twice a day, it is difficult to notice unless I look closely or compare photos with previous weeks. Going to cut off a couple small pieces of calyx's shortly and will post a couple pictures from my scope. Speaking of those, they're starting to give me some nice colours, slight purples, neon greens and yellows, the photos don't really do them justice under the warm lighting but I will try and take a couple pics in some white light when I chop her down in a couple weeks time. It is also quite difficult to illustrate the size of some of these colas, the one near the back on the right, is about 19 inches long, and about 4 inches wide. Looks like 2 ounces of bud hanging on her and I have had to lasso some string around it and a couple other colas as they were falling down. Bringing EC slowly down to around 1.2 - I think I will cut nutrient strength down to about 0.8 EC in a few days time to try and hurry her along a bit. I will only be flushing for 3 days at the end of this grow, they will also be in the dark for 2 days at the end with low temps (mid teens in ºC) to preserve terpenes, oils and other cannabinoids. Thanks for stopping by!

What can I say this thunder fuck is so smooth to smoke, I just can't stop growing it! I am in uncharted waters here because I've gone full organic no till. This will be my first time using grassroots living soil pots and first time using this brand new soil mix. Also to help with costs I decided to make a 5 gal tea reactor, to grow out the EM1 which will save me about 75% on all microbe products, and also save on feralization when used in conjunction with foliar sprays. Wish me luck!

Welcome growfessors to another episode of growfessor theatre, 4x4 edition! Flush time, just water for LSD, Green Crack and Mandarin dreams!! Do-si-dos gets another week of feed before flush. I'm very impressed with the bud sizes, trichomes and smell of each plant, mandarin dreams is especially ooozing with resin, it's almost like liquid soap touching your skin. The Mars-Hydro TSW2000 light has been killing it! This light delivers way more than expected! Thanks for stopping by growfessors, tune in next time 👽🌳💚

Start of week buds fattening will update. Added recharge on day 53.

Arrivato fresco fresco dalla Spagna si pianta subito. 12/01 12 h in acqua 13/01 l'ho messo in un tovagliolo di carta umido ed è è uscita la prima parte di radice ed è stato trapiantato in un vasetto. 14/01 e uscito dalla terra sfogliando le prime 2 foglioline tonde. Questa è una super partenza 72 h e già cerca la luce.

Had to tie up branches to take the weight of these heavy ass nugs, can’t want to taste this smell orangey as f**k! 🔥🔥🔥 started flush midway through the week.

This week they had more water and more knf soil soak , Including fish amino acid , lacto basilicas, WSK, WCAP , OHN . Bud booster this week probably bac bloom stimulatior.

ESPAÑOL: Hola chicos, espero se encuentren muy bien! Esta semana las chicas han mejorado un poco, se detuvo el amarillamiento, descubri que Cal-Max de Grotek interfiere en la composicion de la linea hesi por lo que las chicas sufren un bloqueo de nutrientes, dejé de utilizarlo y las chicas han continuado con su desarrollo relativamente normal. ya estan en su 5ta semana de floracion, Tutankhamon ha producido una cantidad gigantesca de resina, Lennon esta engordando a toda velocidad y aun le quedan 6 semanas aprox. Anubis a pesar de su color intenso amarillo palido esta engordando bastante bien y su produccion de resina es inimaginable y ya solo que quedan 2 semanas y media aprox. espero que a partir de ahora ya no surjan mas problemas y que las chicas terminen su floracion de la mejor manera. esto es todo por esta semana chicos, espero que se encuentren muy bien ! INGLES: Hello guys, I hope you are very well! This week the girls have improved a little, the yellowing stopped, I discovered that Cal-Max from Grotek interferes with the composition of the hesi line so the girls suffer a nutrient lock, I stopped using it and the girls have continued with their relatively normal development. They are already in their 5th week of flowering, Tutankhamun has produced a gigantic amount of resin, Lennon is gaining weight at full speed and he still has about 6 weeks left. Anubis, despite its intense pale yellow color, is gaining weight quite well and its resin production is unimaginable and there are only about 2 and a half weeks left. I hope that from now on no more problems arise and that the girls finish their flowering in the best way. This is it for this week guys, I hope you are doing well!

Early update from week 7. As hoped, she's doing great again. I just put in a lot of nutrients and she loves it. A little LST on the BPP2 and a little defoliation on the BPP.

This plant is Definitely a weird one growing one fingered leaves. Light penetration is perfect so I didn't have to train. Unfortunately 3 weeks of her life she grew under the older plants, and didn't get much if any light exposure. The buds would have been phenomenal had I got 3 more weeks out of her

17/07/22 des fruits apparaisse dans une grande majorité ,j'ai essayer de scrog les chessecake et les wedding glues en bon débutant j ai casser une branche que j'ai essayer de reparer a la macgyver tout sa en photos!.je suis très satisfait du milieu de cette semaine ! 22/07/22 demain dernier jour de la semaines des fruit a 90% partout plutot bien lancer mais pas très homogene j'ai pas mal de progrés a faire^^ très bonnes semaine a suivre

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

7I wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smileI wish they didn't make me do this to get a smile

As usual my grows take forever. Healthy... just slow growth. Ph is good. Runoff EC is good. Lights 150w at 17". Humidity 59 Temps 79