Germination (4-6 to 16-6) Melonade Runtz Feminized - Dutch Passion. Genetics: Melonade (Lemon Z x Lemon Tree) x Runtz. 20-25% THC, 50% Indica, 50% Sativa. Flowering time: 9-10 weeks. Aroma and Flavor: Pungent, lemon and tropical fruits with creamy, pastry-like notes. Effects: Powerful, euphoric high with a strong narcotic-like effect, suitable for experienced users. 4-6 Today i put the seed into a wet coffee filter to germinate. 6-6 After 48 i checked on the seed and the root grew really long! A bit longer to my liking so i should have checked sooner. I put the seed in a 0.5L pot filled with some Plagron Lightmix, it stays in the dark till she is above the ground. I made a feed with 0.5 gr Calcium and 0.2 ml Rootstimulator per liter. EC: 0.4 PH: 5.8, and watered the pot a bit. 7-6 After 24 hours she is above the ground and i removed the plastic foil covering the pot. 8-6 Temperature: 19.5 to 24.1 degrees Humidity: 41% to 70% Watering: 20 ml. 9-6 Temperature: 18.8 to 23.4 degrees Humidity: 62% to 74% 10-6 Temperature: 19.4 to 23.9 degrees Humidity: 60% to 74% 11-6 Temperature: 19.4 to 25.2 degrees Humidity: 43% to 71% Watering: 25 ml. 12-6 Temperature: 21.2 to 25.4 degrees Humidity: 44% to 60% 13-6 Temperature: 22.2 to 25.9 degrees Humidity: 44% to 71% Watering: 15 ml. 14-6 Watering: 40 ml. A week later and today is the last day of the germination phase. She is a bit of a stretcher, as she is growing taller then the other plants in the pre-grow cabin.

Vamos familia, aquí os traigo la sexta semana y primera de floración de estas Royal Gorilla de RoyalQueenSeeds. Vaya color, y van con buen ritmo. La temperatura maxima es de 26.5 y la Mínima se queda en 23.5. En cuanto a la humedad está estable en 50% Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Hasta aquí es todo , espero que lo disfrutéis, buenos humos 💨💨

Journal de Culture : Frost 1 (Semaine 7) ​Génétique : Frostbanger (F2 Perso) | Système : Autopot 20L ​📣 Remerciements ​Un immense merci à toutes les personnes qui suivent ce journal depuis le début ! Un grand merci en particulier à @MIA_BIOTABS et à Mrs_Larimar pour leurs précieux conseils toujours aussi utiles. Merci à tous pour votre soutien ! ​🛠️ Configuration Technique & Gestion de l'Espace ​Éclairage : Lumatek ATS 300W Pro (réglé à 75%) ​Distance lampe/canopée : En adéquation avec la fin de la pousse verticale ​Climat : Jour : 25°C / Nuit : 21°C ​Humidité (HR) : 50% (parfaitement stabilisée par le déshumidificateur) ​Tente : 1m80 de hauteur ​📅 Évolution & Floraison : Fin du Stretch ​Le mot du cultivateur : Petite confession pour cette mise à jour : je suis parti en vacances à Rimini sur un coup de tête avec ma famille ! Pas d'inquiétude pour la box, l'Autopot gère l'arrosage en mon absence et le déshumidificateur veille au grain pour garder un climat impeccable. Les automatisations, ça change la vie ! ​État de la plante & Morphologie : ​Hauteur actuelle : 83 cm (+ 5 cm depuis la semaine dernière). Aileron, étirez-vous : Bonne nouvelle pour la gestion de l'espace dans la tente, elle a enfin arrêté son stretch ! On va pouvoir respirer par rapport à la hauteur de la lampe. ​Évolution des fleurs : Les têtes sont maintenant bien formées et commencent à prendre une très jolie structure. ​Observation couleur : Petit détail intéressant, elle commençait à changer de couleur pour tirer vers le violet, mais elle s'est arrêtée en chemin. C'est dommage, j'aurais bien aimé voir ce phéno s'exprimer à fond, mais elle reste magnifique ! ​Nutrition (Le passage au PK) : Comme prévu après la période d'assèchement du pot, je suis passé à l'action côté booster : ​Apport manuel de 4 ml de PK 5-8 de chez Biotabs dans 0,75 cl d'eau (pH 6,3). ​Cet apport ciblé arrive pile au bon moment pour soutenir l'explosion des premiers vrais pompons maintenant que la structure ne bouge plus en hauteur. Le reste du temps, l'Autopot continue de distribuer l'eau claire. ​Résumé visuel : La canopée stabilisée présente une belle homogénéité. Les sites de floraison profitent pleinement de l'espace libéré lors de la dernière mise à jour. Les fleurs se densifient, la résine commence à s'inviter sur les petites feuilles et l'aspect général respire la santé. La phase de gonflement est officiellement lancée ! une bientôt ❤️❤️

Hands down she has raced to be the first plant that will be harvested, which I do not mind because this cheese scent she has is driving me crazy, my senses are drooling 🤤 and crying, patience is a skill reserved for the brave. Love how the LST has given me about 3 main stems, the crystallisation on this plant is incredible, it looks like I am growing it in an indoor environment. All she’s doing is fattening her buds, I hope she gives about 50grams of dry bud. I need to start formulating a questionnaire, I am going to dedicate 10grams to researching how well I grow bud, that is six strains, 10grams each, that is about 60 joints weighing a gram each. At the end I want to open my own community weed forum.

Nutrient deficiency? or over feeding nutrients? Normally feed 3days nutirents 1 day ph water messed up my feeds which made me feed nutrients for 4 days instead of my intended 3days which may of caused yellow tips. only on a couple of the leaves i (THINK) i might of caught pretty early Flushed with ph 5.9 water saturday 18-1-25 as of 19/1/25 24 hours later no signs of it getting worse or on any other leaves decided to cut big fan leaves of to try and direct the growth more to the nodes going to leave it an hour or 2 to see how she reacts to what ive done then if all is good i shall feed with nutrient Nutrient mix 5litres H202 2.5ml Silicate 3g Calmag 5ml Micro 5ml grow 5ml bloom 4ml Ph 0.1ml 22-1-25 no feed for two days to see how it holds up..(Its holdin up) last feed was 20-1-25 just some light LSTand defoliation today on 22jan just to keep all branches around the same level the banana cookies that was planted same time seems to be a bit behind and is not on the same path

Weather wise this week was great up until the weekend. Rain, rain and more fecking rain which will cause my nemesis the slugs to appear again like a thief in the night.. The smell at the moment is very pungent, very much like the stink from a zoo! Weird but sure is strong. I have just added some bat shit into the feed along with a table spoon of molasses to give her sugar boost. I can wait for this lady to finish she is looking real nice if I do say so my self. Lol.

She's finally gone into flower turn into a nice little bush and was a breeze to train I have started to do leaf stripping cause of environmental and cause of how fat the leaves are let's be honest a blurple light isn't the best light 🤣 but seriously this should be a lesson for me I like challenges we will see what we can yeild. Edit- been topped dressed on 1-10-24

Day 65, 14th of November 2020: Growing so so much topping does not stop her! Really nice! Strong tall girl and growing aggresively similar to my Gelato from the same breeder Original Sensible Seeds. I hope she calms down a bit and stop growing it is enough thank you ;) 😅😅 Pre flowers and pistils are and nicely forming! Fertilization happens every 2nd day with the ratio and mix above. I set the lamp 15 minutes shorter to switch off earlier so she receives 11:45 of darkness. I would like to imitate the nature when longer nights come with time till the 4th week (when she will receive 13 hours darkness a day 15 minutes minus 4 times = 1hour) so every week 15 min longer darkness for 4 weeks and then back to 12/12 to have bigger buds from the 4th week....

She’s the queen of the tent, sweet smelling sticky and chunking up nicely . Needs a defol this week . Feeeding her up now and looking for her to stack on the weight

This thing is insane! Rapidly growing everyday. I need to keep it under control for another for about 30 more days before I can fit it in my other tent.

Siamo ormai entrati nella 3º settimana di fioritura e sto cercando di tenere un EC intorno a 1650 e PH 6.5, le piante crescono sane e con pochi problemi di carenze, con questi vasi bevono più o meno il 3º giorno e si asciugano benissimo…dovrò sicuramente comprarne altri molto presto!😎 Sono curioso di vedere fino a che punto posso spingere questi semi di Anesia! La prossima settimana saliamo a 1.750 💚🌱

Ya empezando la 5 semana ahora empieza el engorde

Week 8 - Day 1 of Flower: flipped to 12/12 today. Also gave them about 4 gallons of water. The first two gallons was a Sprouted Seed Tea (SST), and the other two gallons was Jay Plantspeaker Quillaja as a wetting agent, then added Rootwise Enzyme Elixer, and Bio-Phos, BuildASoil Big 6, BuildABloom, Coconut Powder, Pure Protein Dry, Fermented Comfrey and Fermented Peach. 3/3/2023 - Day 5 Flower: made up a foliar spray of Tweetmint and warm water and spayed the plants at lights out for their weekly IPM. Also, they needed to be watered again. I made another SST and mixed in the same ingredients as the last watering, but I did not add the fermented Comfrey or Peach, but I added the Yah-Whey this time. Also, added some TeaCo Super Tea blend and some FishShit as well. Loaded the girls up for their stretch.

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

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Day 44 - March 25, 2021 Started noticing a couple gnats in our house. It's been warm outside and they are starting to come out. Unfortunately saw some in the room as well and can only assume there are many more feeding on the ladies. Gave foliar spray using Bee Safe Organicide. Gave all the girls a thorough haircut today and worked on trying to get canopy under control. Vegatation was very thick. Doesn't look like deficency issue with F7 is spreading. Continuing to watch for any changes indicating the problem is still ongoing. Gave foliar spray (1:00AM): * Bee Safe Organicide Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 10:45PM * 2.21 EC * 5.9 PH * 73.94F (with ambient temps being higher, res temp is higher as well) * ~13-gallons remaining before topping off * Added ~5-gallons (solution mixed 3/24) (11:00PM) * 2.16 EC (after topping off) * 5.9 pH (after topping off) * 73.40F (after topping off) * ~18-gallons remaining (after topping off) Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) ** 1:00AM-4:00AM (Reduced light intensity to 50% for foliar spray) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars Off 24-hours Air control: 69F-82F (80F AVG) (Lights On) 66F (Lights Off) 50%-65% (56% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1181PPM CO2 (Lights On) ~690PPM CO2 (Lights Off) Day 45 - March 26, 2021 Didn't do much in the garden today. Tucked a couple branches under trellis netting and that's pretty much it. Hoping will have the trellis on each cage filled out by next weekend. Nutrient deficiency with F7 seems to be resolved. New growth not showing any issues. Found a dead gnat on a leaf. Bee Safe foliar spray seems to have done the trick. Continuing to keep close eye out for pests. Mixed of nutrient solution (11:00PM): ** 50-gallons RO water (0.06 EC) ** 10ML h2o2 per gallon ** 5ML Botanicare Cal-mag+ per gallon (0.54 EC after Cal-Mag) ** 0.5ML Mills Nutrients Vitalize per gallon ** 10ML Mills Nutrients Basis A & B per gallon ** 4ML Mills Nutrients Start-R per gallon ** 1.25ML pHup per gallon (bring PH from ~5.1 to 5.8) ** ~2.02 EC ** 5.8/5.9 pH Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 11:30PM * 2.16 EC * 5.8/5.9 PH * 73.94F (with ambient temps being higher, res temp is higher as well) * ~10-gallons remaining * Didn't top off today Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (81F AVG) (Lights On) 66F (Lights Off) 50%-65% (60% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1268PPM CO2 (Lights On) ~620PPM CO2 (Lights Off) Day 46 - March 27, 2021 Did some more pruning to remove thick foliage and open up nodes. Continuing to tuck branches through trellis. Expect will be ready to go into flower in apprxomately a week. CO2 tank ran out and co2 levels dropped to 330PPM for the first couple of hours of lights on. Replaced tank at 10AM. Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * topped off with ~18-gallons * tested 9:30PM * 2.06 EC * 5.9 PH * 73.94F (with ambient temps being higher, res temp is higher as well) * ~20-gallons remaining Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (80F AVG) (Lights On) 66F (Lights Off) 55%-70% (64% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1397PPM CO2 (Lights On) ~330PPM CO2 (Lights Off) Day 47 - March 28, 2021 Didn't do much in the garden today. Deficiency issue on new growth is continuing to newest nodes on F7 and is starting to show in F6, F2, and F3 as well. Also noticed a few leaves with small tip of nutrient burn. Flushed F2, F3, F6, and F7 until runoff EC was equal to input. F1,F4, F5, and F8 look good but will flush those tomorrow as well just in case. Flushed with: * RO Water (starting EC 0.05) * 0.5ML Mills Nutrients Vitalize per gallon * 5ML Cal-Mag+ per gallon * pH 5.8 * EC 0.53 Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 8:30PM * 2.06 EC * 5.9 PH * 74.48F * ~13-gallons remaining Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (79F AVG) (Lights On) 66F (Lights Off) 55%-70% (65% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1312PPM CO2 (Lights On) ~1292PPM CO2 (Lights Off) Day 48 - March 29, 2021 Flushed F1, F4, F5, and F8 until runoff EC was equal to input. Gave Foliar Spray Foliar Spray: * 1 Gallon RO Water (EC 0.08) * 10ML CalMag+ per Gallon * 0.5ML Mills Nutrients Vitalize per gallon * PH 6.0 * EC 1.19 Flush with: * RO Water (starting EC 0.05) * 0.5ML Mills Nutrients Vitalize per gallon * 5ML Cal-Mag+ per gallon * pH 5.8 * EC 0.54 Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 8:30PM * 2.06 EC * 5.9 PH * 73.40F * Topped off to approximately 20-gallons Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (78F AVG) (Lights On) 66F (Lights Off) 55%-70% (67% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1301PPM CO2 (Lights On) ~1117PPM CO2 (Lights Off) [Goofed up on setting timer for UV bars and were on during dark period causing CO2 to stay on.] Day 49 - March 30, 2021 No work in the garden today. Gave Foliar Spray Foliar Spray: * 1 Gallon RO Water (EC 0.08) * 10ML CalMag+ per Gallon * 0.5ML Mills Nutrients Vitalize per gallon * PH 6.0 * EC 1.19 Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 8:00PM * 2.05 EC * 5.9 PH * 73.76F * Topped off to approximately 20-gallons Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (76F AVG) (Lights On) 66F (Lights Off) 55%-70% (70% AVG) humidity (Lights On) 60%-75% RH (Lights Off) ~1288PPM CO2 (Lights On) ~793PPM CO2 (Lights Off) Day 50 - March 31, 2021 Deficiency Issues: Still having issues with what looks like Zinc deficiency. Also found 4 of 5 fan leaves with Calcium deficiency over the last couple of days. Runoff is PH 5.8 and EC is 0.78. EC in runoff has been very low. I'm pretty confident it's not a PH issue at this point. Seems plants are rapidily soaking up the nutrients in the water. I also found 8 or 9 fan leaves with slight nutrient burn a few days ago. Nutrient burn seems to have tapered off and haven't noticed any spreading. Going to try adding an additional watering to the schedule to see what happens. I'm hesitant to mix stronger nutrients due to nutrient burn I started to see earlier this week. At the same time, need to figure out the cause of the nutrient deficiencies. I'm currently using the Light Feeding Schedule from Mills Nutrients (says "light", but is still 2.06 EC including 0.54 EC for water + CalMag) Need to switch to flower soon but need to figure out deficiency issues before the switch. Defoliation: * Removed quite a bit of fan leaves suffocating nodes. Girls are 10lbs lighter. Despite the nutrient issues, the girls have been filling out fast. Did a timelapse over the past couple days and can see significant growth in a short period. Gave Foliar Spray Foliar Spray: * 1 Gallon RO Water (EC 0.08) * 10ML CalMag+ per Gallon * 0.5ML Mills Nutrients Vitalize per gallon * PH 6.0 * EC 1.19 Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 8:00PM-8:10PM ~0.33 gallons per pot Reservoir: * tested 11:30PM * 2.06 EC * 5.9 PH * 73.40F * Topped off to approximately 20-gallons Lights: * Gavita LEDs On 18-hours (10:00AM - 4:00AM) ** 10:00AM Lights On (100%) ** 11:00AM Reduced intensity to 50% for foliar spray ** 12:00PM Increase intensity to 75% ** 1:00PM Increased intensity to 100% * Gavita LEDs Off 6-hours (4:00AM - 10:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (77F AVG) (Lights On) 66F (Lights Off) 55%-70% (67% AVG) humidity (Lights On) 60%-75% RH (Lights Off) ~1574PPM CO2 (Lights On) ~880PPM CO2 (Lights Off)

Day 36 I guess I can call it bloom. Nice strong pistils shooting all over. She is the tallest so far making me raise my lights a little more. Day 38 Now Californian Snow is the tallest :) Ztrawberry stopped at a perfect height, it looks like she's gonna develop into a beautiful lady. Fat white pistils all over 😻 Day 39 Happy girl got 2500ml cm bg bb bh 6.3ph ~1EC She is perfect, symmetrical, stopped stretching at about 50cm, flowers are mostly leveled, branches are strong, leaves point up unless I water. I think she's also turning purple. Beaut 😻 Day 42 I think I saw some burnt tips but she still looks super happy and healthy. The most vigorous plant I've had.

I transplanted them on Friday 17 and topped them on day 21. I am planning to build mainlines out of all 5. So far they're looking pretty good. Thanks for stopping by and have a safe holiday.

- LAS PLANTAS YA NO SE ALIMENTAN . Y SE LES HIZO LAVADO DE RAÍZ , EMPEZÓ EL COLOR AMARILLO EN SUS HOJAS Y OTRAS A CAERSE - NO SE VEN CARENCIAS NI EXCESOS - el hps de 600 MANTIENE MI AMBIENTE SIN MAYORES INCONVENIENTES -SALIERON COGOLLOS DENSOS Y RESINOSOS , BASTANTES DUROS SOBRE TODO LOS GORILLA GLUE

Great grow!! Should end up with over a half a pound off of these 2 and the little I harvested about 2 weeks ago. I got 2 oounces dry off of her. They smell amazing and are covered with trichomes