Very happy thought I'd ruined the buds with a homemade spider mite spray. Brought contaminated top up soil into the grow room without sterilising, lesson learned. Spray worked perfectly though and washes off fine with no bad residue or taste. My spray bottle is going to forever smell like garlic I think 🙈😂

Good

Final week. Shes on ripen for 3 days already

Último día del año y último día de la semana 9 de las bebas. Está semana que entra sería la última de la white quizás llegando a fin de semana corte solo las puntas y dejé una semana más los bajos o en caso que le falte la esperaré una semana más (creo que es lo mejor) viene muy compacta y olorosa, resina no veo tanto como en blue moby que viene con menos producción pero que dejó en segundo y tercer lugar a las otras en producción de resina le quedan 2 semanas veremos cómo sigue evolucionando pero estoy fascinado. Moby dick viene atrasada pero avanzando de a poco espero que no quede estancada y me quedé flaca seguro se atraso por el estrés de que se cortó su punta principal hace un par de semanas pero bueno creo que vale la pena esperar a todas!! Desde lo personal les deseo que empiecen un año mejor al que paso y que les traiga más lindas experiencias con el cultivo y los más bellos cogollos, un avance hacia la legalización en dónde todavía se les prohíbe está hermosa planta, felices fiestas y próspero año nuevo! Buenos humos! 🙏💪

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Welp, These took a Bit longer than my Cream Cookies and still didn't fill out quite like I thought they would.. But they are still a very beautiful strain and a joy to have grown. They smell stronger and way more lovely than the cream cookies thou just a beautiful aroma I'd really like to grow these again Indoors where I can control them easier. (Sadly they dried into wispy nothings and we turned them all into bubble hash) #3 took the hit of some aphids and ended up in a frozen hash pile in our fridge along with two cream cookies from the run. It's frozen weight combined was 203g. *will update with dry*

Crecen fuertes. Detecte una mosca blanca :c

I grow in pure coco with organic nutrients

Things are going fast, HulkBerry 1 look close to the end, I will start to wash it soon. I think the cookies gelato familly will end in something like 2 weeks, but not sure. Feel free to let me know what u think about. I got a real problem with the Royal Bluematic 2, buds are so small and weak. Not sure to bring it AT the end.

4/9/25 This plant is beyond frosty. Stacking up super nicely. Seems like she will yeild quite a bit. My only gripe is that she has like no smell at all. Using the foil as a soil cover is working quite nicely. the top soil is getting filled with roots quite well. Top dressed 1tsp of Bloom 1tsp microbe charge 1tsp bloom

Day 32: Girls look very dark green, some clawing, and sides sticking up. These girls got too much N and maybe a little light stress. I have rearranged my gilrs in the tent, so these a getting a little less light. I have also flushed them again with some plain ph'ed water, and gave the half-strength nutes after

24/05/2023 - 78 days since my little girl sprouted from a seed🌱 Tenth week of my Jack Herer 🌿🌾🌲 Nutrients: Advanced Nutrients Flawless Finish - 2ml - I rinse the plants two weeks before harvesting so that they have a clean taste and smell 😋👃🌴 -------------------------------------------------- ----------------------------------- Lights: I use a Mars Hydro TS1000 - 150w full spectrum, great light for vegetation and flowering use 💡💡 Training: No training is needed this week In general: As I already wrote, Jack did not go well for me this round. I still don't know what caused the burnt leaves. Whether it was a small watering and high heat, or a bad reaction to nutrients. The pH of the soil was fine. Its buds are very weathered and have many leaves in them 😒 In a week I will gradually harvest the girls 🌿🌾 Thanks for the likes and you can follow me on Twitter 🐦: @ Targona666 See you soon 😍

Day 65: All good guys, I started flushing Sour Stomper rest of them need more time the next ones will be Critical Orange Punch and Lemon Zkittle. Bids are developing nicely. Need more time a bit to get ready. Water intake 200 ml a day. 600W LED, 18 hours on 6 hours off humidity is low approx 35 percent. Day 67: I started flushing Critical Orange Punch as well.

Day 46, 30th of October 2020: Crazy... I went on holiday only for 6 days so my friend looked after them.... Guys... They fucking grew 20 cm it is crazy look at them I had to do LST and now giving them darkness one day and switch the lamp to 12/12.... They went totally crazy.... The smell is coming as well I bought extraction fan system with filter as well.... It is not a question all good and everything will continue the same way well from next week pump a lot of nutrition ;) Cheers!

Not really much going on here, plant did not grow much because of some defolation work i did this week, looks healthy and glad tho. Next sunday i will switch lights, what do you think about going 16-8, 14-10, 12-12 weeks?

beautiful genetics, except that I left it for a few days too close to led ... and I bleached one or two leaflets! otherwise I can only have nice words for this genetics in cbd. it grows a lot even indoors, makes many branches, and has a pungent smell, the life cycle is almost over, it will be a good harvest

Hi and welcome to a new grow :) Unfortunately I have to start over because of a malfunction I had with the automation of the heater. I basically cooked my plants. But now we‘re back with three new Ladys, wich I hope will turn out as beautiful as in the picture. I planted four, just in case one of them doen‘t make it. I will choose the 3 faster ones of course. The medium I use is mixed with soilles media (peat ,vermiculite) with additives. It‘s basically sythetic but it still has microbes. The nutriensolution is also mixed syntheticaly. I‘m not going to explain everything right now but at this stage i‘m feeding the plant low amounts of the Veg formular that was recomended in different studies. The EC is about 1350. PH of the Media and the Water is about 5.8. I also have a CO2 bag but unfortunately it was cooked by my heater 😬let‘s hope the funghi survived. Since oxigen is very important for the roots I am pumping Air throught the watercontainer. It helps keeping it clean as well. I do have Hidrogen Peroxide as well, but i‘m not sure if i should use it for the extra oxigen. If any of you have experience with it feel free to comment! I will keep you updated on the plantgroth over the week but for now everything is setup. Have a nice Weekend 🌱 Update: The first three girls are coming out pretty fast. InicuallyI though the 4th seedling didn‘t make it but she came out a day later. that was part of the planing so we’ll see. The first three are roughly the same age too wich is awesome. Right now i‘m giving them around 200 ppfd/24h. The Temperature and humidity are stable at 26°C and 90% humidity (as in picture) now it‘s lowered to 75% wich means the VPD is roughly 8,5. Let‘s hope I don‘t cook them this time 🙏 Oh and the CO2 bag is alive. Update: I‘m experimenting with VPD and I figured out, that the leaf experiences a different VPD due to cooling of the leaf while water is transpiring. So with LEDs the leaves don‘t warm up from the missing infra red but luckily my Light gets pretty warm so i put it up hight and brighten it to 40%. The seedlings stopped growing at a Lux of 8000 so i put them on 13000 with a leaf VPD of 0,6. I used a VPD calculater and have the following environment: 27,5°C and 60% Humidity. The leafs are ≈24 degrees (if i measured correctly) and the caculator spits out 0,6-0,8 wich would be ideal. Let‘s see how they grow since they are seedlings i‘m not sure if this stresses them. If you have any comment, please feel free to wright is down so i can adjust if necessary.