Week 17 (23-6 t/m 29-6) 23-6 Temperatuur: 31.2 graden (licht aan) 23.2 graden (licht uit) Luchtvochtigheid: 71% (hoogste) 53% (laagste) Dit is de laatste week bloei, dus geef ik geen voeding meer. Watergift: (Flush) 1100 ml EC: 0.0 PH: 6.4 24-6 Temperatuur: 31.5 graden (licht aan) 24.2 graden (licht uit) Luchtvochtigheid: 67% (hoogste) 50% (laagste) Watergift: (Flush) 1100 ml EC: 0.0 PH: 6.4 25-6 Temperatuur: 32.9 graden (licht aan) 25.2 graden (licht uit) Luchtvochtigheid: 72% (hoogste) 47% (laagste) Watergift: (Flush) 1100 ml EC: 0.0 PH: 6.4 26-6 Temperatuur: 33.5 graden (licht aan) 23.1 graden (licht uit) Luchtvochtigheid: 68% (hoogste) 48% (laagste) Watergift: (Flush) 1100 ml EC: 0.0 PH: 6.4 27-6 Temperatuur: 31.2 graden (licht aan) 21.7 graden (licht uit) Luchtvochtigheid: 61% (hoogste) 47% (laagste) Watergift: (Flush) 1100 ml EC: 0.0 PH: 6.4 28-6 Temperatuur: 30.1 graden (licht aan) 22.4 graden (licht uit) Luchtvochtigheid: 75% (hoogste) 47% (laagste) Watergift: (Flush) 1100 ml EC: 0.0 PH: 6.4 29-6 Temperatuur: 31.4 graden (licht aan) 23.7 graden (licht uit) Luchtvochtigheid: 75% (hoogste) 53% (laagste) Yes! Ze kan geoogst worden! Watergift: (Flush) 1100 ml EC: 0.0 PH: 6.4

September 10th, Like I mentioned in my last post. She started flowering. But i was truly amazed The flowering of rhis girl from the week is nothing like what i have witnesssed so far in my grows outdoor. Usually outdoors, plants preflower and stack up for ages.(4weeks or so) This Killer kush seems to skip time and pop out nugs all at once. Tiny balls all over this plant now. Buds ..inside, outside and.in between Cant wait so wait to see how this one progresses during weeks to come ! Weather was stormy for a few days. Fortunatly more sunshine is on it's way...according to weather model.

Día 54 (10/03) Riego 1,25 Litro H20 + Wholly Base 2,5 ml/l + Solid Green 2 ml/l + Early Flower 1,25 ml/l de Gen1:11 TDS 1033 PPMs - pH 6,51 Día 55 (11/03) Defoliation time! Con la increíble ayuda de @miyaguiokpolilla, @dogdoctorofficial y @growwithflow420 hice la defoliación recomendada exactamente después de 21 días cambiada a 12/12 Consejos de defoliación - Elimine menos del 20% de las hojas de abanico y solo las hojas de abanico - Retirar el tercio inferior de la planta, incluidas las ramitas. - Retirar las hojas en forma de abanico dirigidas hacia el interior de la planta. - Retire las hojas de abanico que dan sombra a los futuros cogollos. Día 56 (12/03) Esperando algo de estrés tras la defoliación... Han crecido 2 centímetros en 24 horas 😍🚀 Día 57 (13/03) Riego 1,25 Litro H20 + Wholly Base 2,5 ml/l + Solid Green 2 ml/l + Early Flower 1,25 ml/l de Gen1:11 TDS 1043 PPMs - pH 7,0 Las plantas muestras una ligera deficiencia de CalMag. Voy a subir el pH a 7.0 en este riego para mejorar la biodisponibilidad Día 58 (14/03) Siguen con su crecimientos, los cogollos formándose y las hojas de azúcar empezando a llenarse de resina Parece que la corrección de la deficiencia de CalMag ha sido muy efectiva! No hay más manchas y las plantas no paran! Día 59 (15/03) Como mañana me voy a Spannabis 😍 voy a adelantar 1 día el riego reduciéndolo a 1 litro Riego 1 Litro H20 + Wholly Base 2,5 ml/l + Solid Green 2 ml/l + Early Flower 1,25 ml/l de Gen1:11 TDS 1080 PPMs - pH 6,57 Día 60 (16/03) Spannabis time! 🚀 Día 61 (17/03) Spannabis time! 🚀 💦Nutrients by Gen1:11 - www.genoneeleven.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae 🎚️Controlled by TrolMaster TCS-1 Tent-X System Main Controller - https://www.trolmaster.com/Products/Details/TCS-1

Planta hermosa, de estructura resistente y cogollos rocosos y repletos de resina. Resistente bien el entrenamiento LST y topping, pide bastante alimentos y te recomiendo lavar bien las raíces antes de cosechar si has usado fertilizantes. Los frutos desprenden una fragancia exquisita y muy dulce.

I gave the 8 Ball Kush and the Blue OG a full 9 weeks in flower , we started the trim yesterday and will finish up today , now it’s cleanup time and get the room reloaded with 8 Ball Kush and Zombie Kush, I will update next weekend on final weight.

Wir sind heute in der 5ten Wochen, 2 Pflanzen wollen irgendwie nicht, ansonsten läuft es recht gut denke ich mal, da es mein erster DWC Grow ist( HYDRO ). Werde die Tage die Heads Bud´s Schneiden und Stecklinge davon machen.

Good start to the week temps dropping slightly. Some light defoliation and tucked a few branches under the trellis for light penatration a little video in there looks like the stretch has calmed down focusing on producing flower now ... 09/12 a little problem went to find my trellis had come of the clips pulling my canopy into one big ball sorted it and put a pic up of the day after so no major problems or snapped branches luckily thanks for reading happy growing

🎃👹👽MONSTERCROPPING RED MANDARINE 👽👹🎃 ____________________________________________________________________________________ 💀 12.3 ... 💀 13.3 ... 💀 14.3 ... 💀 15.3 ... 💀 16.3 ... 💀 17.3 I have not forgotten about this beauty, but it was too downtown to constantly move it to take pictures. But I am attaching the last photos, before cutting (and certainly some other macro) on like this! yeah! 💀 18.3 ____________________________________________________________________________________________ 📜👀 A look at the details of what I'm growing 👀📜 🍊💚 Red Mandarine F1 🍊💚 by 🌱🍭 Sweet Seeds 🍭🌱 📋 Details ⚧ Gender ▪️ Feminised ➰ Genes ▪️ 55% Indica / 45% Sativa 🎄 Genetics ▪️ Red Poison Auto (SWS39) хCalifornia Orange x Skunk hybrid) 🚜Harvest ▪️ 400 - 500 g / m² 🌷Flowering ▪️ 49 - 63 days ✨THC ▪️ 16% ✅CBD ▪️ 0,2% 🏡Room Type ▪️ Indoor 🌄Room Type ▪️ Outdoor 🕋Room Type ▪️ N/D 🎂Release Year ▪️ 2019 __________________________________________________________________________ 👀📷🥇 Follow the best photos on Instagram 🥇📷👀 https://www.instagram.com/dreamit420/ 🔻🔻🔻Leave a comment with your opinions if you pass by here🔻🔻🔻 🤟🤗💚Thanks and Enjoy growth 💚🤗🤟

Day 8 Flower (Day 50) The first day of the second week of flower and the strech is real. The right girl is getting pretty damn big and keeps on drinking a lot. I gave her another 3 liters of pH 6.4 water today. On the other hand, the left girl drinks hardly anything, so I didn't need to water her. I also did some lollipopping today and got rid of a whole bunch of undergrowth. I saved some clones off the big girl but no clue what to do with them. We'll see what happens. Day 9 Flower (Day 51) Chill day today. All I did was to give each girl 3 liters of pH 6.5 water and snap a few pics. Day 10 Flower (Day 52) Today I finally got around to re-organize the small tent to move the light as high up as possible to give the canopy some additional space. I moved the exhaust fan to outside the tent, so it was out of the way for the light, and then put the circulation fan below the canopy, blowing upward. Finally, I adjusted the light so it was reading about 900 PPFD in the center of the canopy. The left girl didn't need any water today, but the right girl got 3 liters of pH 6.2 water. Day 11 Flower (Day 53) They're back! The fungus gnats are back in the tent, although I'm not surprised, and let me tell you why. When the light for my tomatoes and peppers broke down, I had to move them so that they get some daylight. It is still cold here (the coldest May around these parts ever, I think), so they have to stay inside even though they usually would have moved outside long ago. The best place to keep them in my apartment is by the window in the same room as my grow tents. The last time I had gnats, I only had enough nematodes to treat my girls, but I didn't do the rest of the apartment even though I should have. So currently, my peppers and tomatoes all have a ton of gnats. Put plants with gnats in the same room as your grow, and it is only a matter of time before the gnats find their way into the tents. So yeah, it wasn't a surprise at all when I noticed the gnats in my tents some days ago. I put up some yellow sticky traps to deal with the adults and ordered more nematodes, enough to treat my girls, plus all other plants in my entire apartment. Twice. The nematodes arrived today, and I mixed up 10 million of them into 6 liters of water and gave each girl half at pH 6.5. That was it for today. Day 12 Flower (Day 54) Exhausting day today. I opened the tents, took pics, closed the tents. Phew! I think I need to lay down for a bit. Day 13 Flower (Day 55) Late last night I decided to supercrop the right girl to slow her down and get the canopy to even out. We're almost at the end of the second week of flower so this is the last training I will do besides some defoliation later on. I think I cropped around 10 branches or so. Today, most of the branches were already standing up again and she was VERY thirsty so I gave her 4.5 liters of pH 6.4 water. The left girl on the other hand didn't need any water. Day 14 Flower (Day 56) The last day of the second week of flower and the right girl is in flower. Early yet so still small and no smell, but they are there. The left girl is in flower as well but isn't as far along, but that isn't unexpected considering the different lights. The left girl is 68 cm tall (18 cm increase in a week), and the right girl is 100 cm tall (23 cm increase in a week) but would have been even taller without super-cropping. I gave each girl three liters of pH 6.3 water and removed the tape from the right girl's broken branch. She looks fully healed, so I removed the supports as well, and she was looking great. However, thinking about it for a second, I put back one of the supports, just in case.

Been very bad & struggling with this diary and many other all at once. Down to 8 full sized plants 1 plant HERMED last week. Overall growth is fine/even. New fan/filter installed to cope with 600w CMh. New test on nutrients -bloombastic -Rezin So

The smell is just gorgeous, ppd favorite for sure like a bag of fresh berries. Guzzlerz starts to purple, whole underside of the leafs are black. Purple Coolies Kush behind of all plants but will be very iced

Day 50 since Germaniation. Genetics are really nice and strong. Even against high amounts of nutritions. Rqs never disappoints but this genetics i like the most so far. Cant wait to see the result

I was quite happy to see no more signs of Cabbage Loopers. I awoke to rain this morning so spent part of the day self-supporting the heavier branch sections. All in all everything is super sexy.

Started flushing the critical auto

Runoff PPMs are at 876/pH 5.9 average last watering, so back in range Gelatos are stretched to 32" now LSDs remain 24" Gelato kolas are starting to show color.

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""