Well as you can see I'm playing with these ladies a lot now ..They are both on week 7 the smaller CBD was just put into a bigger pot and will be stretched out a bit then left to reach right up for the sky not looking for big gains with this one but we shall see.. The blue cheese she is my baby and living the good life .. she has been trained nicely and will be left to go straight up as well as with in reason I do have neighbours .. The team at Dinafem were my first plants to ever run and I've been running them ever since love the genetics so much

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Last week of veg before flip gave them a good defoliation and double feed ..... will defoliate again at day 20 of flower , but so far so good am pleased with the veg stage

The girls are doing well lots of bud sites and smells heavenly. The mars sp250 is alot stronger light then I gave it credit for and had to raise it a little higher then my burple lights as it was trying to burn the top of one. Defoliated some of the bigger leaves that were blocking some bud sites but other than that I pretty much let them do their thing

Hello friends! Welcome back to the enchanted gardens of PEAKY These sweets seem to develop differently My God ... It looks like a meteor fell from uranium into my tent It gives off radiation ... be careful!😎

Really massive little compact strain

PATIENCE, cooler temps and straight water for 2 weeks, girls smell sweeter each time I have a look inside. getting close to $60 for 2 complete grows with my SF 2000. my next grow I will factor in all costs from dirt, seeds to all electricity and nutrients. Some amber showing, won’t be too much longer I’m ready but the girls have other plans apparently, so I wait...waiting for the sun, waiting for the sun.... 2/28/21. .... well shit, the girls won't be ready together, which means I will need to use another tent to start drying. my place will be packed up in boxes ready for the movers except for a drying tent for the kush...its gonna be close. Later that day... one plant harvested at 1.4lbs wet (22.5 oz) Life is good

Not sure about the pin down technique, although I will carry on until flowering. It definitely requires more practice. Seems like the plant is willing to live, and the thing is I have only 3 months until my holiday, so there is no time to start over with an another grow. The genetics in this one isn't the best in my opinion, one stem grows really odd leaves. I will wait a bit more and if the strange shaped leaves are keeping growing, then I will have to cut off that branch.

Day 22 : Today was repotting day! I prepared my living soil demetheart system, I added terralba products and dynomyco mycorrhizae! I added molasses mixed with reverse osmosis water, elycitor and humic and fulvic acids! my tco is in preparation for 24 hours, I use the tco pack from terralba, to which I add many elements and bacteria Hollywood x white runtz is in great shape! nothing to say Day 28 : Here is the end of week 4, everything is going very well!! I will carry out the topping on day 29 and incorporate the TCO + defoliation!! this variety is very vigorous, very compact and has small internodal spaces! but I have a good feeling, it seems resistant to stress! we will see a few days after the topping

Hallo Freunde 👋 die Mädels sind 63 Tage Alt und Gehen in Woche 10! Die große Banana ist ein Monster! Papaya machen sich auch so langsam. Habe zu spät geschnitten und triebe entfernt , wird beim nächsten anders man lernt nur dazu! Sonst keine Probleme. Wieder eine wahnsinnige Reise! Die große Dame bekommt jetzt nur noch Wasser und Denke das sie nächste Woche so weit ist ! Keep Green and grow High ✌️🍀💚🍀

09/18/2021 At battle with catapillar this week Hand picking them off, some bud rot caused by catapillar's . Light defoiliating dying, yellowing leaves. Hand picking is tedious but well worth the effort. Feeding neptunes harvest 2-3-1 this is last week of feeding. Drinking lots of water this week gave each plant 2.0 gallons of Neptunes Harvest 2-3-1 hope it works out couldn't find fox farms, or big buds but I'm sure they will be fine.....will feed again on Friday then plain water for 3-4 weeks till harvest. Feeding should get them through the next two weeks then watering plain water should flush anything not needed by plants. Girls are thirsty this week, just in time for feeding! Neptunes Harvest seems to do the trick. Pistils piling on like crazy so one more feeding on Friday Of 2-3-1 and Week 7-8 banana peel tea, she should start Fattening up,and fading nicely by week 8-9.🙏🏻 The NPK value for banana skins is 0.6-0.4-11.5. But this is the value for dried banana skins. (I will make tea from peels) not sure of N-P-K Is for peel tea? But it works well in my opinion. Day # 6 week 6 applied gypsum today gypsum should help a little with PH issues,7.0 a little to alkaline but she will grow, but she thrive at 6.2 PH

Day 14 for these kre8 Genetics CandyMan regular seeds but I'm feeling confident I've got at least 2 girls growing 🤞🏻 been on mild feed of monkey Nutrients every couple days and seem to be enjoying it 😏 gave them just water today. The pots will be topped up with soil in the next day or so then I can take the pins out and start topping & training them 😍 Update: I've now just topped them on Day 19

@twenty20mendocino A-Team R&D Update ~ Let’s Go day 24 of flower 🌸 an we are looking amazing! Ladies are starting to stack an oh my we getting a lot of frost going on! starting to get some gassy stanky notes of em too😍 ! We are only into week 4 ladies an gents we still got a few weeks to go of stacking! Keep your eyes peeled for next week’s update yall , peace love a positive to all y’all , an have an amazing productive day ! Cheers an blaze on !!

Es folgen weitere Bilder wenn sie getrocknet ist

Eccoci di nuovo qui!!! Super eccitato per questa nuova collab con Spliff Seed, che mi ha dato l’opportunità di testare questa nuova genetica e di condividere i progressi con tutti voi!!! Come sempre partiamo nei bicchieri per poi travasare.. Questa volta verrà svolto tutto sotto la Lumatek Zeus 465 ProC, mi aspetto molto da questo ciclo!! Genetica che inizialmente mi aveva colpito per la resina che aveva ma una volta grindata e fumata mi ha lasciato senza parole: - Sapore intenso con retrogusto dolce sembra uno sciroppo!!! - Effetto devastante corporeo ti manda direttamente sul divano! La consiglio a tutti per la facilità di coltivazione!!! Grazie a tutti per il supporto ❤️🍀🔥

Soaptini another winner from lit farms. The plants leaves are turning a black color very pretty with deep purple stems. It’s loving up to its name with some buds that are so frosty they look white like soap and they smell of a gas soap smell. Also the nugs are kinda small with an odd bud structure and odd growth structure. I think it has a little to much breeding and crossing done to it without stableazing the genetics. I’m sure if grown several plants there would be a huge pheno variation good thing I got a pretty plant from the lottery. Great smell should be some gas.

Que pasa familia, vamos con la cosecha de estas Papaya Sherbet de FastBuds. Por dónde empezar, las flores son bien compactas y van repletas de resina, tienen tonos verdes bastante bonitos, y tiene unos aromas afrutados bastante marcados con toques cremosos. Es muy sencilla de cultivar, es de ciclo de 2 meses y medio, pero bien agradecida. Hasta aquí todo, Buenos humos 💨💨💨

Another closeup video for you guys, I know we all love them. Last photo is a little sneak peek of a tester, harvest will look crazy - promised. Smells like a fruit Tart with berries and Lemon, Persian pie absolutely killed it. Thank you Greenhouse seeds.