2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Termino de la segunda semana de floración y las niñas no pierden el tiempo. La malla scrog ya fue situada y esta siendo acomodada de a poco para obtener la mejor producción y como si fuera poco, las niñas tienen un muy buen color y se están adaptando bien tanto a la malla como a los nuevos fertilizantes. Seguimos manteniendo la dosis de fertilizantes tanto en la gelato auto como en las sublimator y amnesia molotov y una vez se terminen de mostrar las flores, agregaremos un aditivo para adicional (a las feminizadas) para que potencien su volumen y sabor, que en este caso será PK Booster de BAC

9/29 Wow that NYCSA is probably the healthiest plant I've ever grown.ive researched more on the strain. I researched flower time and it is 8 to 10 weeks! Tichs are starting to turn milky. Hairs are turning a little amber. I FIMMED this and it turned out beautiful with like five colas. The little 5lb kush is done. It's about the densest buds I've grown. We have a few days of good weather so I may leave it for a few days. I was considering another app of plant doctor but held off. If I see ANY wpm I'll apply it. Going to hit the NYCSA with cha ching again. Probably one more feed. Grow big should've been taken out a couple weeks ago. Seeing this plant grow so healthy solidifies my new grow area. I even found two or three aphids! Obviously there are no earwigs or their wouldn't be ANY aphids. Nor buds near the stalk. I've got two little quarter size buds at the bade of the plant and those would be gone. Blueberry turned out great. Can't believe it made it through the fusarium outbreak. UPDATE: I think the little 9lb kush is done. Both by the trichs and the shape of the bud. The next few days are supposed to be really good though so I might just let it ride. That NYCSA has continued to surprise me. Strong odor resinous buds. Still blond hairs that have just started to change but the buds are really stacking up. This is one of the healthiest plants I've had so its going the distance. The buds are swelling and trichs getting milking bit the hairs are still blond. I'm hoping for another both swell. When I FIMMED this it ended up coning out perfect with like five tops. I'll have to post a picture of the limb skeleton. I can't take the anxiety with the rippers and letting things go till perfection so I've started keeping the plants inside. I guess that's one benefit if a small grow bag lol. 10 gal us a lot easier to move than 50. I was upset that I lost so much but in very grateful for what I still have. 9/30 Harvested the 9lb kush today. Trichomes say it's done. The buds haven't been getting any bigger either. It is coated in trichomes (approx 15%-20% amber) and extremely dense. I'll save details for the harvest report. FED the NYCSA kf gallon. I used only Cha Ching and Silica. I'm not sure if it should be hit one more time with plant doctor or not. I'm not seeing mold or pm. It says up till day OF harvest and has instructions for medical cannabis. The trichs on this NYCSA have swelled and are filled in super milky. Amber on leaves. I think the FIM might be deceiving me as so many tops are going to be smaller than one large cola. It also has blond hairs all over it. They ARE pulling in a bit but the plant doesnt look ready to me. No senescence but It's one of the healthiest plants I've ever grown. Temps dip into the low 30s at night. I will keep thos diary updated. I need to get my ass in gear and trim my blueberry so I can do the harvest report. I grew it last year too. Turned out better this year. Still have buds on tge blueberry plant that I want to use to show the damage earwigs do. Even to fully mature buds with tons of trichs that should protect them. 10/1 This may have been a shitty season and I lost ALMOST everything to fusarium but I'm grateful for what I do have. Quality over quantity this time. The NYCSA goes inside at night. Trichs are all milky af buds are dense af. This girl has a week or two left. I'm young to pet nature dictate this harvest. I know this is a long flowering strain so I'm letting it ride. Very few of the yellow pistols started turning red. 10/2 Another super sunny day. I'm go8ng to have to water soon. Wondering if I should do one more app of plant doctor before I harvest. I saw a smidgen of pm on a very lower inner leaf. I think the plant needs a week or two. It's tricky. Trichs say its done and buds are swelled but the pistols haven't changed color yet. A VERY few have but hardly any and I've got all milky trichs and super rock hard buds. Frost warning today and tomorrow but I'm putting the last one inside at night. I check it multiple times a day. Should be OK. 10/3 Last couple nights were freezing! Car was all iced up this morning. Good thing I put the last girl in the barn. She was absolutely perfect except for a few spots of pm down low. I bring a loupe everyday things change so fast. However since this plant is so healthy and has no problems I'm letting it ride until I feel it's perfect. Another hard frost tonight but the days are AWESOME. Just pure sun! Pistols are receding but haven't changed colors yet amd are still light orange. Some have died and turned red. The trichomes are full and milky with a few amber. I know that NYCSA is a long flowering strain. Looking at the structure of the flowers and the thickness if it's not done it's really close. I gave it some water today. About half a gallon. I was wondering I'd I should give it one more dose of plant doctor before I harvest. I think I've got a week or so left. I'm uncertain if I should use it or not. The pm was very space amd I wiped it off with a paper towel and alkaline water. The lb kush I harvested looks and smells amazing. I can't wait to try it. 10/4 Super cold last night. I waited to bring the plantvout until 8am and the temp only rose to 35 from 31 at 6am. Checked trichs again amd they look perfect. My only hesitation is that pistols haven't changed color and I noticed that SOME die and turn a bright red. The others pulled into the bud but stayed a more yellowish color than orange. I can't find any I go on this strain New York City Skunk Ape. I found the breeder which is from Maine. I think it's goonie genetics and its 10 week flower I think. This is a tough one. We have some sunny weather coming and it won't be too cold. I'll bring it inside at night. I did notice negligible amounts of pm on lower portion of plant. I defoliated but I didn't want to do the plant doctor as it's been so cold. Last few days have been all hard freezes. Below 30°F. I'll check on this later. If possible I'd like it to get all the sun it can. I'm just running out of time. Checked the relative humidity with not a cloud in sight and iys all sunny but the rh is 88%! No wonder we have a problem with mold. It I didn't think I'd be harvesting in a couple days I'd use plant doctor again. I'll make that decision later. It will at least get today's sunshine. EDIT: TODAY WAS THE DAY. She came down. Trichs were milky turning amber and the buds were fat as hell. I'm not risking anything else and I doubt I'd gain anything anyway. This girls telling me she's done so I listened. I'll be updating that harvest report soon. So excited for this strain!

I harvested it a little earlier than it should have but shouldn't spoil the fun. I don't now how much is the total weight. Bud I think it's about close to 180-200grams

Hello Guys ! Hope you all are doing well during this beautiful summer. I am very happy to share pics and videos of this new flowering week 😁😎 The ladies are very strong and pretty despite these hot temperatures but I am very confident with the plants. It doesn't smell so much for the moment.

Day 1 Just planted seed direct to coco coir, bagged for humidity, no exhaust fan as of yet, COBs dimmed to 1/10th power...let's see how this goes also sprinkled seed holes with great white mychorrhizae. Day 3- Sproutling Partially Unearthed , Installed AC Infinity T6 Day 4- I have little sproutlings 100% germination and 3 above ground girls. Combated low humidity yesterday with an asthma nebulizer lol. Decided to invest in a humidifier today. So far so good.👍🏾 Day 5- Today coco was a little dry. Gave them Cal-Mag and Great White myko mixed water Ph balanced to 5.8.

So this one started slow but healthy. She was a sativa that expected much more leg space and warmer humid climate which i fixed later. Needs LsT. I hit it with my concoction to keep her short it worked. Dense nugs especially for sativa hybrid. Not loud but tropical sweet. I needed 11 weeks min for flower could run 12 even though I seeded mine. Tiny sativa seeds like the original ibl bred by jaffa seeds uk and brought to me by Pevseeds. Not bad for under a cheap 120w led quantum and 3 gallon grow bag. I should have went with 5. The roots were amazing dense.

if you love blueberry then this strain is for u,absolute nug fest.Nice colas.wasnt the tallest plant but short and stocky with decent meat on the bones.Very resistant

Buds are finally fattening up! I've decided to open her main stems a little bit, lack of space in the grow tent made training almost impossible but using this plate method I'm able to expand her arms while keeping her in a quite small surface. Guess what, the plate works really well, it was easy to set up and it helps a lot improving the airflow around the main colas.

Cepa de poco estiramiento en preforación. En vegetativo es bastante resistente y vigorosa dentro de los normal. En floración a mi gusto le faltó punch en el engorde.

Gorgeous buds from this hard diesel pheno #2 guys, very lemony and sweetie aroma, I will see how good this lady is when I smoke her but looks and smells incredible, I enjoy with every strain of Gea seeds didn't get a bad plant so far, let's see how this lady keeps developing those NUGS!!! ❤️💚

This plant is growing well, I transplanted them this week into 3 gallon pots from 1 gallon pots. Structure on this plant is pretty nice and visually pleasing to the eye lol.

7° settimana di fioritura tt procede bene

De una semana a otra su desarrollo fue muy notorio. Se encuentra perfecta, sin ningún problema. En esta etapa esta teniendo olor a weed muy fuerte. Esta muy resinosa.

1 moth passed from germination. In result we have 12 strong plants and 4 reduced. They begin presenting wonderful scent! Increased my led on 40 % of power and it seems great. Buen fin de semana a todos !

Day 72: 600W LED, 18 hours on 6 hours off the same with ventilation. Ferilization is the same. Water intake also remains the same 200ml per day. Humidity approx 35 percent. Day 74: I just checked the trichomes OMG this is quick one it is done getting amber I need to harvest after tomorrow. So she wont get water today tomorrow and Friday is harvest.

✌️🎃 Thank you for checking my cultivation. 🤤 I can’t wait to harvest. 🛁 Washing times.

First week of 12/12 You can see the buds forming now The blueberry cupcake is stretching wild In comparison with the blue zushi. I also put in the dehumidifier and set it on 65% If the cupcake gets too high I'll just add the second trellis earlier.

End of week 7! This plant is looking amazing. Done giving it all the nutrients just the base and overdrive to finish her off. 2 more weeks I'd say and we are harvesting!