Day 49: Like is told you guys last week, I went on vacation for 3 weeks, so my friend took care of my plants. Buds are starting to show up. I do have one plant which is light greenish. Might be from overwatering or shortage of nutrients. I don't know the reasons since I have not been taking care of them. The weather/climate still sucked. Alot of colds and rains. Until next week.

******************************************** Week 12 Mid flower (week 6 flower) ******************************************** Light cycle=12/12 Light Power=160w 67% Extractor controller settings (during lights on). High temp= 26c Temp step=0c High Rh= 50% Rh step=0% Speed max=10 Speed min=3 Extractor controller settings (during lights off). High temp= 21c Temp step=0c High Rh= 55% Rh step=0% Speed max=10 Speed min=3 Smart controller settings (during lights on). Lights on=9.00am Radiator on= -22.5c Radiator off=+23.0c Top fan on= Smart controller settings (during lights off). Lights off=9.00pm Radiator on=-18.5c Radiator off=+19.0c Top fan on VPD aim=1.0-1.5 DLI aim=40-45 EC aim=1.0-1.8 PH aim=6.0-6.5 💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧 NPK= 10-19-30 Method= Automatic Feed=Flower nutes Neutralise=0.1ml/L Plagron bloom=5ml/L Plagron Power buds=1ml/L Green Sensation=1ml/L Easy Ph Up=0.053ml/L (1ml=23 drops, each drop is 0.043ml) Ec=1.75 PH=6.3/6.2 Runs=18 Run times=4mins (0.3L each) Gap times=16 mins Total runtime=72mins(5.5L each) Total flowrate= 152ml/min(76ml/min each) Auto start time=10.00am Auto stop time=15.44pm 💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧 ******************************************** ******************************************** 📅22/12/24 Sunday (Day 78)(flower day 38) 📋 💧 Automatic mid bloom nutes Ph up=0.64ml, 15 drops Ec=1.75 PH=6.3/6.2 Volume=12L Volume left=1L Volume used=11L(152ml/min) Volume each=5.5L(76ml/min) Runoff. Total runoff=0.7L Ec=4.0 PH=/6.1 💧 📅23/12/24 Monday (Day 79)(flower day 39) 📋 📅24/12/24 Tuesday (Day 80)(flower day 40) 📋 Christmas Eve. 📅25/12/24 Wednesday (Day 81)(flower day 41) 📋Christmas Day. Have a merry Christmas. 📅26/12/24 Thursday (Day 82)(flower day 42) 📋 Boxing Day. 💧 Automatic mid bloom nutes Ph up=0.69ml, 16 drops Ec=1.8 PH=6.3/6.2 Volume=13L Volume left=2L Volume used=11L(152ml/min) Volume each=5.5L(76ml/min) Runoff. Total runoff=1.0L Ec=4.4 PH=/6.2 💧 Leaf tips are getting burnt and runoff Ec is climbing so just water next time, should have really just been water this cycle. 📅27/12/24 Friday (Day 83)(flower day 43) 📋 📅28/12/24 Saturday (Day 84)(flower day 44) 📋H=93cm D=22cm Dli=46 8 ppfd=1080 ******************************************** Weekly roundup. 📋 Good week considering how close she is to the light, she does have some damage from the light but not as much as I was expecting. Next week she'll get a little less intensity when I turn the power down a bit. The weather is still fairly cold so used 34.17Kw £8.64 on the radiator. Back soon. Take it easy. ********************************************

This plant is just gorgeous! She is really starting to develop nice bud sites. My super soil mix is keeping her super healthy and happy!! I keep making sure to stay on top of her as far as treating her with OMRI fungicides. I made the tough decision to chop down my Mango Sapphire this week as she had a lot of powdery mildew starting. It was too close to this lady and decided the risk was too great. I can’t wait to see what another week brings!! Onward! 😎🔥🍻🍿🌱

Week 9 started with having to bring down tent once again it was only for one day it did happen to rain lightly that night honestly plants are resilient af they have been thrown a lil bit of everything an they bounce back no problem all deficiencies it had in Earlier weeks seem to go away right away buds are just stacking up nice.. I do notice the plant in 7 gal fabric pot is bigger than 5 gal pot.. also all my training to keep it spread out kinda goes out the window everytime I have to remove scrog net..we are about halfway there..gonna flower for about 8-10 weeks

The last week under the sun. Now there will be three days of darkness. Then it’s time to harvest. The flowers give off a sweet, fruity, berry-like scent with a hint of skunk. This plant has grown into a beautiful purple blossom. It would have needed another 1–2 weeks to reach perfection. However, since I’ll be away for two weeks and the plant also produced 2–3 male flowers, I’m harvesting it now to get the most out of it. Light Power: 92% Day 114 Flower #63 Photoshooting

10th December Increased the EC to 2.75…the plant is looking healthy so far and developed the first Trichomes 🤩

A-Day 53 (into week 8) B&C-Day 49 (end of week 7) A - She is stacking well, and feeling greasy! The smell is coming on stronger everyday, frost is just starting to build up. There is a distinct transition as the leaves go through veg to early flower to late flower. Uniform all around, not very tall, but she is hardy and thiccc! B- Full flower now, so many tops! She has shown to be a slower flower developer than A, but the flowers she are developing are almost completely uniform all across the 🌱! I assume because there are so many they will be a little slower. I put her ties back on, and the middle shoots exploded with new stretch and growth, so she is still in that stretch/flower stage. C- what can I say, patience is a virtue. She is looking lush and vigorous. With this being a slightly longer to finish auto strain, she has had time to recover from a) being a runt, B) having slow growth, c) having almost no training and lighter nutrient feeds her whole existence. This is also my control for Topping, she was not, A and B were. This strain is clearly hardy and able to withstand multiple techniques, we’ll see what the end the result is compared to her sisters. All three plants received a full nutrient feed x2 this week. That was: 3 gallons dechlorinated water mixed with 12 ml Micro, 12 ml Bud, 20 ml Grow, and 6 ml Sensi Extra CalMag, PH’d to 6.2. And another very low feeding of 1/3 that mixture mixed into 3 gallons of water. Ive heard many growers who instead of just watering, they will do a much lower strength of nutrients included in their watering, and ultimately never not feed their plants nutrients until flush. Thoughts?

😓😥😢attaque botrytis 😭 j'ai du couper les plus belle tête qui montré des signe de moisissures 😓 j'ai peur que sa se propage 🤯

📆 Semana 3: Con la EC ya controlada y estable, se termina de ajustar el pH en el coco, afinando la disponibilidad de nutrientes. Las raíces se mantienen blancas y activas, con buena colonización del sustrato y absorción uniforme. Las plantas consolidan el estiramiento, mostrando ya preflores claras y una estructura más definida. El verde lima se va equilibrando hacia un tono más sano, manteniendo buena turgencia y orientación hacia la luz. Se intensifican los primeros aromas y la entrada en floración es ya evidente, con una transición limpia tras la fase de ajuste. Seguimos creciendo fuerte 💪

Hey guys ! finaly half/one more week to go, i'll push them into 61 days of flowering The Zake#1 is already done (30% amber), but i have to cut the whole squad since im drying inside the growbox. See you in 1 week for the last half flowering week ✌️ Happy Christmas to everyone

From sprout, I've been watering every 3-4 days. Been very consistent with this. Yellow tips been appearing. I think it could be Magnesium or Calcium deficiency. Hit it with some Calmag with iron.

She has begun flowering stretch more lst Pre buds formed 4 days later flowering in full effect

May 3, 2024 Day 78 This week is the last week of nutrients before a week or so of flushing. I'm still not seeing any fade, but most of the buds are turning a beautiful purple pink color. At least on Tropicana Cookies. Tropicana C isn't quite there yet. Maybe a few days behind. Some purple is starting to show though. No watering for today. I have the reservoirs full of diluted nutrients. I added a half gallon of plain PHed water yesterday. However, these plants aren't taking in much. The soil is getting saturated and the plants aren't drinking as much. I think it may be nutrient lockout. Or just the age of the plants. They slow down when nearing the end. How near, I don't really know. Maybe a week or so before harvest? Either way, the soil moisture used to be around 49% to 51% saturation. Now it's at 57% which has been unheard of through the whole run. I just can't tell which problem it is. Tropicana Cookies looks great! Lots of bud through the whole plant, not big, but getting denser and slightly fatter. As I said before, her buds are getting super purple with tons of orange pistils. The trichomes are a mix of clear, cloudy and amber. Mostly clear and cloudy with amber being about 5% throughout. Tropicana C looks great as well. One top is leaning hard and no wonder. It's covered top to bottom is dense bud. Still not big buds by any stretch of the imagination, but they seem hard as rocks. I haven't felt them to find out. I'll figure that out on trimming day after drying. She doesn't have the purple coloring like her sister, at least not yet. Some buds are starting to show the purple, but nowhere near as much. The lighting will be changed at some point within the week. Maybe closer to the end of the week. The light is far away enough where I shouldn't get any trichome issues. I definitely won't be getting light stress at all, so I may leave the light as it is for as long as I can. The environment is still a little wonky. The temp is the same, hovering between 75° and 76°. I'm doing my best to keep it under 77° as 78° can cause a potential breakdown of trichomes. Gotta be careful at this crucial time. Grow System Environment: Temp: 73.2° RH: 55.0% VPD: 1.23 kPa May 4, 2024 May the 4th be with you! Star Wars all the way! A little bit of a thing for these two today. First, I need to comment on how purple these buds are getting. Second, I think they only have days left. Maybe a week. But based on how they look and their trichomes, I wouldn't be surprised if they were ready in 5 days. That being said, I think it's time to start flushing. There are a couple reasons I think they are almost ready. For one, they have nearly all orange pistils. Just a few colas here and there. They still have a decent amount of white, but they will be gone later today if not tomorrow. The trichomes are a good 60% cloudy, 30% clear and about 5% amber. I'm leaving 5% out for margin of error. Finally, this could also be a lockout, they are taking significantly less water. At this stage it is to be expected, but I do have a nutrient issue with both of them. So it could be that as well. Or a combo of the two. All that being said, I think it's safe to say they are ready for flushing. Tropicana Cookies is noticably stronger than her sister, or her buds are just heavier. But, this lady has no hard leaning branches. Some leaning a bit inward, but that was how I trained them. On the other hand, Tropicana C has quite a few branches leaning hard. I had to prop one up against a stronger cola to keep it up in place. Another is leaning inward so I have that propped between 2 other colas to prevent light blockage. Both plants look kinda terrible right now with top leaves having spots everywhere from too much of something. Or lack of Phosphorus? Something. I need to learn this stuff. I think the lesson learned here is to make sure to time everything properly and not pay attention to every detail in a feeding schedule. The one I used was based on 3 weeks of veg and 6 weeks of flower. I had 5 weeks of veg and am now on week 6. Flowering seems to be on point, but the longer veg stage messed me up. So I will be following the feeding schedule provided by Advanced Nutrients. Just cut the nutrients way down. The light power will be dropped this week. I plan on dropping it 10% mid week and another 10% by the end of the week. The DLI should drop back down to 40 mol/m²/d inside and 38 mol/m²/d outside. By end of week, I plan to have the DLI down to 38 mol/m²/d. However, I have 2 other plants in the tent that are about a week behind these two. So I may have to change my plans for lighting and leaves it up for the rest of the week and drop it down to 38 mol/m²/d next week. But I don't think I have more than a week left. Oh well. I also won't be able to run a dark period. The plants are too big to fit in a 2x2 tent. So they will be harvested right away. I'll just keep the temp down below 75° for the rest of the week. The other strain is Banana Purple Punch, so it should benefit from the lower temps as well. I shouldn't have any trouble with mold or anything. The plants come out of the tent everyday and the airflow is fantastic. So the high humidity shouldn't be much of an issue. I'll do everything I can to drop it as much as possible anyway. Grow System Environment: Temp: 74.1° RH: 57.9% VPD: 1.19 kPa May 5, 2024 A bit to do today. Both ladies need top offs. Tropicana Cookies much less so. Tropicana C is almost empty while Tropicana Cookies is about half full. She has to be super close to harvest. The trichomes show it as well. Not quite there, but almost. That being said, I'm switching to Flawless Finish for the rest of the grow. They will need about a week of flushing and then I think they will be done. Tropicana Cookies is looking great! Her buds swelled up to an acceptable size. They definitely aren't nearly as big as my Gorilla Cookies, but that was an insane plant. Anyway, I'm not seeing any signs of fading. Just nutrient burn. However, I rarely get fade. Maybe it will start later this week. Anyway, I'll have to clean the reservoirs out. I may have to do that today. Just plain water though, then next watering will be Flawless Finish. Tropicana C is definitely putting on weight. Her colas are starting to pull more and more branches down. Leaning everywhere. I have 2 that will completely fold over, so I have to wrap them around other branch leaves to lock them in place. Her buds aren't purple though. It's starting to come through now, but nowhere near as her sister plant. However, her pistils are like glowing orange. Beautiful. She still has a good 40% white pistils. I like to see a minimum of 75%, but I rely mostly on the trichomes. Regardless of color, she is still quite the beauty. Both ladies are extremely strong to the nose now. Even with my exhaust fan at 100% power, it still doesn't pull all the smell out. It's could also be the combo of these two and the two Banana Purple Punch plants in there tent as well. Either way, they look and smell great! The lighting will have to stay where it is for the rest of the week. I don't think it will be a problem. I have the light 24" away from the plants and shouldn't have any stress at all. The reason being is those two Banana Purple Punch plants. They still have a good 10 days left while these two only have about 5 to 7 days left. So it will stay exactly where it is. The environment is driving me nuts. I can't for the life of me get the humidity to stay below 60%. I swear it's the gods messing with my shit. Lol. The temp is still controllable. I want to bring it to 74°, but then I risk higher humidity. But if I crank the heat, the temp could get too high and damage the trichomes. What a sticky place to be. I'll just have to open the door periodically throughout the day while this humidity is out of control. Grow System Environment: Temp: 73.0° RH: 59.8% VPD: 1.09 kPa May 6, 2024 Not a lot going on today. No watering needed, no light changes, nothing. Just some pics and environmental adjustments. Tropicana Cookies looks great! She's very close to harvest. I'll be checking trichomes again in 2 days. I'd say 5 days left on her. She just needs more amber trichomes. And she could do with less clear trichomes. Although, the majority of them are cloudy, this is an indica dominant strain. So I like a little more amber than that of a sativa. Honestly, it levels out the harvest time. Sativa's take slightly longer so less amber means less time. More amber on the indica strains takes slightly longer than their shorter lifespan. So they tend to level out for harvest time. Also, what I thought was more damage to the leaves, I'm starting to think it's the start of the fade. Even with the Flawless Finish flushing out the extra nutrients, the leaves are still showing "damage". So maybe it's the start of senescence? Or residual nutrients still in the soil. Probably that now that I think about it. It will take days for the issue to subside. I'd also like to note that the damaged leaves aren't crispy at all. They still feel like they are very healthy. So instead of cutting them off like I planned, I'm going to leave them on and use them for a timeline for the rest of the plants. Tropicana C also looks great! Her branches are bending over all over the place. Just the outside ones and taller colas. They may be small buds, but they are solid and a plethora of them. I'm finally seeing purple. So she is definitely the same phenotype, just got slightly delayed by probably 3 days or so. I'd say give this one another 5 to 7 days. It's could be up to 10 days which is no big deal as I have other plants in the tent that won't be done for another week or 2. So I'm not too worried. Both ladies have a crisp and fruity aroma. Even though it's a crispy smell, it's strong and engulfs the smell gland things in a citrus, sweet, fruity, I guess gassy and earthy aroma. They aren't taking as much water in anymore, so it's definitely nearly time. I don't really see it doing much right away. As I said earlier, Tropicana Cookies is still advancing in the nutrient burn. Maybe it was a deficiency the whole time? Someone mentioned missing Phosphorus which I think is Big Bud that I used at the complete wrong time. We aren't changing the light at all. The other 2 plants in the tent need it to be where it is for another week, and these 2 should be done in an week or less. The environment is still wonky with the temp at 75° and the humidity at 58% and bouncing into 60%. I managed to get my room down to 48% earlier, but now it's up to 52%. I'll have to do my best to keep up with the temperature in the tent. Try to keep it below 75° if I can. Also, I really cannot get the temperature to drop below 67° at night. The average is and has been 68° for the majority of the grow. However, last night's humidity was absolutely insane with an average of 67%. I really can't stand weather sometimes. It's a good thing I pull my plants out everyday and inspect them. Otherwise I think I'd have some mold issues. So far we are good to go. I'm also opening the tent periodically throughout the day to let more dry air in and clear out the moisture to room level. Today should be good with the lower temps outside and in turn lower humidity. Grow System Environment: Temp: 73.8° RH: 62.4% VPD: 1.05 kPa May 7, 2024 Not a lot going on today. I might top off the reservoirs tonight, but mostly tomorrow. So leaf damage continues to spread. I swear this better be senescence. It's weird, because it's spotty. All over the place. Like not rust, but yellow and orange. It still looks like leaf damage to me. I don't know. As I've said before, I don't get a lot of fade from my plants, so I'm not really sure what to look for. for example is this spotting. The timing is right for senescence, but I'm not really seeing solid color change. Is that "fade"? I can't see it as fade. It still looks like damage. I guess I'll see what happens over the next few days. Tropicana Cookies looks great! Her buds are almost all purple now. Some of the lowers are still green, but I don't expect the whole plant to turn purple. However, it may happen over the next few days. Tropicana C is weighing down a ton of tops now. They are falling over like dominos. Some are bending half way over. The tallest one has essentially collapsed under its own weight. I used some plant ties to hold it up with other tops. I had to weave a couple tops together just to keep them from falling over when I placed it back in the tent. Then they untangled and flopped over against the other plants and tent wall. A promising sign of some super dense buds. Considering how small they are, they must be heavy indeed. Super dense. I can't wait! The smell is so strong now that it's permeating through my air sealed door into the rest of the house when the tent is open. I even have the exhaust fan, that runs to the outside of the house, at full power. And an AC unit that exhausts outside as well. Still the smell is wonderfully overwhelming. Excellent! Lighting is staying the same until the end of the week. Then I'll be dropping it down to 36 mol/m²/d for the rest of the grow. It's to accommodate the other strain in the tent. The environment is getting better. The humidity my room dropped down to 42%, so the humidity in the tent dropped to 54%. Not good at all, but much better than 60%+ for days. The temp is at 76°. I'd still like to see it at 74°, but I'll be able to do that when I get the humidity under control. Grow System Environment: Temp: 73.7° RH: 54.6% VPD: 1.25 kPa May 8, 2024 Trichome Day! We only have pictures today. I'll do a video for next week. Speaking of next week, after checking the trichomes, I believe these two will be done by the end of next week. I know I'm pushing it off a bit, but they just aren't ready yet. Tropicana Cookies has started to fade. Her sugar leaves and some of the fan leaves are developing beautiful dark purples. The rest of the leaves are starting to lighten up as well. We are looking super good. Tropicana C on the other hand is a bit behind. I'd say 3 days maybe 4. No idea why, but she is. Also, her branches have to be weak AF to be bending all over the place. The buds aren't that big or heavy. So yah, just a weak plant. Definitely from my nutrient regimen. She smells great though and she is getting more purple in her buds, so she will hopefully turn out like her sister plant with super bright purple buds. I think they are the same phenotype, so this one should turn purple too. I think. One issue I may run into is timing. I need to harvest both plants at the same time so I can fit the 4 sour diesels I have in the 3x3 with the other 2 that are currently going. It might be a tight call. If they are indeed 4 days apart, I may overdo it with Tropicana Cookies and possibly underdog it with Tropicana C. We shall see. The lighting is just going to stay the same. There's no point in dropping it now. The other plants in the tent still need it and these 2 are so close to being done, it really won't matter. The environment is a nightmare. I cannot get the humidity below 60% now. It may go down later this afternoon, but not enough. It's maddening. I'll just keep opening the tent every couple hours to let fresh air in, even though the exhaust fan is running full speed. Grow System Environment: Temp: 74.0° RH: 57.6% VPD: 1.19 kPa May 9, 2024 It's the last day of week 11 and we are embarking on the final few days. Nothing to do today, but there are a few notes I'd like to make. Tropicana Cookies has mere days left. Her buds are almost all purple now from top to bottom. Not only that, but the leaves are also turning purple. Some of the bigger fans leaves are starting to fade. It started with the damaged ones and is slowly working through the other leaves. Just a few at a time. Something I noticed is how much water her container is holding. I may have to pull the wicks. I guess it makes the most sense. I'll do that later today. Mostly because the bottom of the container is dripping saturated. I'm worried it will cause some last minute over watering issues. Tropicana C is falling all over the place. And I mean all over. She has some weird bud growth as well. Some of the tops bent over, but not only that, folded over themselves where the tops are now facing the bottom of the container. It looks like I missed something when placing the plant back in the tent. It's just strange how she is doing this and the other one isn't at all. But this one... I feel like all these bending branches are going to impact the light coverage for the whole plant. Especially the outside where the branches are just falling over. I don't have a support like that, and the other tops are too weak to hold the outside branches up. I'll just have to deal with it. If it comes out messed up and larfy, I'll just use it for concentrate. I'm starting to get an idea of the potential yield. Both plants have tons of buds, but Tropicana C seems to have less dense buds, even though she in bending over everywhere. I'd say that Tropicana Cookies has a potential for 3 oz dried and trimmed. Tropicana C might yield 2.5 oz, but I'm thinking closer to 2 oz. We shall see. She still has a few days more. Maybe a week. The light is the same. It's kind of a good thing. The leaning branches still need light, and they are about 2" lower than the light. That dropped the DLI a little and now I don't have to worry. However, it didn't do anything for Tropicana Cookies. The environment is still kind of wonky. Day time temps are around 76° with the humidity still high at 55%. The night temp was really good though. The average was finally down to 66°. So that's good for the last few days. Grow System Environment: Temp: 72.6° RH: 58.6% VPD: 1.11 kPa

Started LST on 3/6 of the plants. Having no issues at all so far👌 Gave her some nutes twice so far at a very low dose. Currently feeding once a week. Soil holds enough nutes as is already. Low dose Cal-Mag in every watering - missed week 2 so I just started week 3

2nd week of stretch in the books and the males showed up on time. We pared the grow down by 4/5 plants. I think I’ve got at least one more male to cull from the lot. Still having serious struggles with getting the females to transition in earnest and cut back all the fan leaves pretty severely to try and encourage some stress induced fruiting. Still very unhappy with the strain to date and now they’re just taking up space. No issues, nor were any expected in this system and I’ll be happy to be done with these. Now it’s just waiting for the finish.

Lacewings seemed to have mostly killed themselves by flying into hot light fixtures. I may have left the UV on which was smart of me :) Done very little to combat if anything but make a sea of carcasses, on the bright side its good nutrition for the soil. Made a concoction of ethanol 70%, equal parts water, and cayenne pepper with a couple of squirts of dish soap. Took around an hour of good scrubbing the entire canopy. Worked a lot more effectively and way cheaper. Scorched earth right now, but it seems to have wiped them out almost entirely very pleased. Attempted a "Fudge I Missed" for the topping. So just time to wait and see how it goes. Question? If I attached a plant to two separate pots but it was connected by rootzone, one has a pH of 7.5 ish the other has 4.5. Would the Intelligence of the plant able to dictate each pot separately to uptake the nutrients best suited to pH or would it still try to draw nitrogen from a pot with a pH where nitrogen struggles to uptake? Food for stoner thought experiments! Another was on my mind. What happens when a plant gets too much light? Well, it burns and curls up leaves. That's the heat radiation, let's remove excess heat, now what? I've always read it's just bad, or not good, but when I look for an explanation on a deeper level it's just bad and you shouldn't do it. So I did. How much can a cannabis plant absorb, 40 moles in a day, ok I'll give it 60 moles. 80 nothing bad ever happened. The answer, finally. Oh great........more questions........ Reactive oxygen species (ROS) are molecules capable of independent existence, containing at least one oxygen atom and one or more unpaired electrons. "Sunlight is the essential source of energy for most photosynthetic organisms, yet sunlight in excess of the organism’s photosynthetic capacity can generate reactive oxygen species (ROS) that lead to cellular damage. To avoid damage, plants respond to high light (HL) by activating photophysical pathways that safely convert excess energy to heat, which is known as nonphotochemical quenching (NPQ) (Rochaix, 2014). While NPQ allows for healthy growth, it also limits the overall photosynthetic efficiency under many conditions. If NPQ were optimized for biomass, yields would improve dramatically, potentially by up to 30% (Kromdijk et al., 2016; Zhu et al., 2010). However, critical information to guide optimization is still lacking, including the molecular origin of NPQ and the mechanism of regulation." What I found most interesting was research pointing out that pH is linked to this defense mechanism. The organism can better facilitate "quenching" when oversaturated with light in a low pH. Now I Know during photosynthesis plants naturally produce exudates (chemicals that are secreted through their roots). Do they have the ability to alter pH themselves using these excretions? Or is that done by the beneficial bacteria? If I can prevent reactive oxygen species from causing damage by "too much light". The extra water needed to keep this level of burn cooled though, I must learn to crawl before I can run. Reactive oxygen species (ROS) are key signaling molecules that enable cells to rapidly respond to different stimuli. In plants, ROS plays a crucial role in abiotic and biotic stress sensing, integration of different environmental signals, and activation of stress-response networks, thus contributing to the establishment of defense mechanisms and plant resilience. Recent advances in the study of ROS signaling in plants include the identification of ROS receptors and key regulatory hubs that connect ROS signaling with other important stress-response signal transduction pathways and hormones, as well as new roles for ROS in organelle-to-organelle and cell-to-cell signaling. Our understanding of how ROS are regulated in cells by balancing production, scavenging, and transport has also increased. In this Review, we discuss these promising developments and how they might be used to increase plant resilience to environmental stress. Temperature stress is one of the major abiotic stresses that adversely affect agricultural productivity worldwide. Temperatures beyond a plant's physiological optimum can trigger significant physiological and biochemical perturbations, reducing plant growth and tolerance to stress. Improving a plant's tolerance to these temperature fluctuations requires a deep understanding of its responses to environmental change. To adapt to temperature fluctuations, plants tailor their acclimatory signal transduction events, specifically, cellular redox state, that are governed by plant hormones, reactive oxygen species (ROS) regulatory systems, and other molecular components. The role of ROS in plants as important signaling molecules during stress acclimation has recently been established. Here, hormone-triggered ROS produced by NADPH oxidases, feedback regulation, and integrated signaling events during temperature stress activate stress-response pathways and induce acclimation or defense mechanisms. At the other extreme, excess ROS accumulation, following temperature-induced oxidative stress, can have negative consequences on plant growth and stress acclimation. The excessive ROS is regulated by the ROS scavenging system, which subsequently promotes plant tolerance. All these signaling events, including crosstalk between hormones and ROS, modify the plant's transcriptomic, metabolomic, and biochemical states and promote plant acclimation, tolerance, and survival. Here, we provide a comprehensive review of the ROS, hormones, and their joint role in shaping a plant's responses to high and low temperatures, and we conclude by outlining hormone/ROS-regulated plant-responsive strategies for developing stress-tolerant crops to combat temperature changes. Onward upward for now. Next! Adenosine triphosphate (ATP) is an energy-carrying molecule known as "the energy currency of life" or "the fuel of life," because it's the universal energy source for all living cells.1 Every living organism consists of cells that rely on ATP for their energy needs. ATP is made by converting the food we eat into energy. It's an essential building block for all life forms. Without ATP, cells wouldn't have the fuel or power to perform functions necessary to stay alive, and they would eventually die. All forms of life rely on ATP to do the things they must do to survive.2 ATP is made of a nitrogen base (adenine) and a sugar molecule (ribose), which create adenosine, plus three phosphate molecules. If adenosine only has one phosphate molecule, it’s called adenosine monophosphate (AMP). If it has two phosphates, it’s called adenosine diphosphate (ADP). Although adenosine is a fundamental part of ATP, when it comes to providing energy to a cell and fueling cellular processes, the phosphate molecules are what really matter. The most energy-loaded composition for adenosine is ATP, which has three phosphates.3 ATP was first discovered in the 1920s. In 1929, Karl Lohmann—a German chemist studying muscle contractions—isolated what we now call adenosine triphosphate in a laboratory. At the time, Lohmann called ATP by a different name. It wasn't until a decade later, in 1939, that Nobel Prize–-winner Fritz Lipmann established that ATP is the universal carrier of energy in all living cells and coined the term "energy-rich phosphate bonds."45 Lipmann focused on phosphate bonds as the key to ATP being the universal energy source for all living cells, because adenosine triphosphate releases energy when one of its three phosphate bonds breaks off to form ADP. ATP is a high-energy molecule with three phosphate bonds; ADP is low-energy with only two phosphate bonds. The Twos and Threes of ATP and ADP Adenosine triphosphate (ATP) becomes adenosine diphosphate (ADP) when one of its three phosphate molecules breaks free and releases energy (“tri” means “three,” while “di” means “two”). Conversely, ADP becomes ATP when a phosphate molecule is added. As part of an ongoing energy cycle, ADP is constantly recycled back into ATP.3 Much like a rechargeable battery with a fluctuating state of charge, ATP represents a fully charged battery, and ADP represents a "low-power mode." Every time a fully charged ATP molecule loses a phosphate bond, it becomes ADP; energy is released via the process of ATP becoming ADP. On the flip side, when a phosphate bond is added, ADP becomes ATP. When ADP becomes ATP, what was previously a low-charged energy adenosine molecule (ADP) becomes fully charged ATP. This energy-creation and energy-depletion cycle happens time and time again, much like your smartphone battery can be recharged countless times during its lifespan. The human body uses molecules held in the fats, proteins, and carbohydrates we eat or drink as sources of energy to make ATP. This happens through a process called hydrolysis . After food is digested, it's synthesized into glucose, which is a form of sugar. Glucose is the main source of fuel that our cells' mitochondria use to convert caloric energy from food into ATP, which is an energy form that can be used by cells. ATP is made via a process called cellular respiration that occurs in the mitochondria of a cell. Mitochondria are tiny subunits within a cell that specialize in extracting energy from the foods we eat and converting it into ATP. Mitochondria can convert glucose into ATP via two different types of cellular respiration: Aerobic (with oxygen) Anaerobic (without oxygen) Aerobic cellular respiration transforms glucose into ATP in a three-step process, as follows: Step 1: Glycolysis Step 2: The Krebs cycle (also called the citric acid cycle) Step 3: Electron transport chain During glycolysis, glucose (i.e., sugar) from food sources is broken down into pyruvate molecules. This is followed by the Krebs cycle, which is an aerobic process that uses oxygen to finish breaking down sugar and harnesses energy into electron carriers that fuel the synthesis of ATP. Lastly, the electron transport chain (ETC) pumps positively charged protons that drive ATP production throughout the mitochondria’s inner membrane.2 ATP can also be produced without oxygen (i.e., anaerobic), which is something plants, algae, and some bacteria do by converting the energy held in sunlight into energy that can be used by a cell via photosynthesis. Anaerobic exercise means that your body is working out "without oxygen." Anaerobic glycolysis occurs in human cells when there isn't enough oxygen available during an anaerobic workout. If no oxygen is present during cellular respiration, pyruvate can't enter the Krebs cycle and is oxidized into lactic acid. In the absence of oxygen, lactic acid fermentation makes ATP anaerobically. The burning sensation you feel in your muscles when you're huffing and puffing during anaerobic high-intensity interval training (HIIT) that maxes out your aerobic capacity or during a strenuous weight-lifting workout is lactic acid, which is used to make ATP via anaerobic glycolysis. During aerobic exercise, mitochondria have enough oxygen to make ATP aerobically. However, when you're out of breath and your cells don’t have enough oxygen to perform cellular respiration aerobically, the process can still happen anaerobically, but it creates a temporary burning sensation in your skeletal muscles. Why ATP Is So Important? ATP is essential for life and makes it possible for us to do the things we do. Without ATP, cells wouldn't be able to use the energy held in food to fuel cellular processes, and an organism couldn't stay alive. As a real-world example, when a car runs out of gas and is parked on the side of the road, the only thing that will make the car drivable again is putting some gasoline back in the tank. For all living cells, ATP is like the gas in a car's fuel tank. Without ATP, cells wouldn't have a source of usable energy, and the organism would die. Eating a well-balanced diet and staying hydrated should give your body all the resources it needs to produce plenty of ATP. Although some athletes may slightly improve their performance by taking supplements or ergonomic aids designed to increase ATP production, it's debatable that oral adenosine triphosphate supplementation actually increases energy. An average cell in the human body uses about 10 million ATP molecules per second and can recycle all of its ATP in less than a minute. Over 24 hours, the human body turns over its weight in ATP. You can last weeks without food. You can last days without water. You can last minutes without oxygen. You can last 16 seconds at most without ATP. Food amounts to one-third of ATP production within the human body.

Flush time.

This is day 80 from seed,I will admit I have messed up,and done some pretty cool things with this grow on my own terms.I just need some guidance on current status of growth referring to harvest,I need some advice on how long she has left before I should harvest her She is a beautiful plant.

Hi everyone 😁 Welcome back on another week update. If you interested to see the full week update. Please revisit this journal as will be posting daily updates. Thank you for such a great support 😊 me and my🍌💜👊 girls loving you all 💚💚💚 So far everything is going great. Girls are healthy and happy. This week I will be increasing nutrients, continue with leaves tucking and perhaps will install scrog net by the end of the week. Wishing you all a wonderful week ✨🍀✨ 06/11 day 29 Watered 500ml each pot and sprayed with water and fish mix solution. 08/11 day 31 Girls started growing faster. Good few cm in to all of the directions in one night. Watered again with 500ml each pot as belive that they need just a bit more time for the first runoff experience. 11/11 day 34 It was a Spa day for the 🍌💜👊 girls. Full package; 1st - removed 7-10 fan leaves from each girl. 2nd - LST on few longer branches 3rd - shower with fish mix and water solution. 4rd - Watering 500ml for Athena and approx 300ml for Xena. 12/11 What a great and a bit busy week with my girls. Perfect response on yesterday selective defoliation and stretching. No signs of any stress. Girls are strong 🍌💜👊💪 So far I am very pleased how this grow going 😁 Thank you all for such a amazing support 😊💜 Peace and love brothers and sisters ✌️💚