Well, another week has passed and it has been growing not exactly as I expected, but they warned me about using only base fertilizers with an inert soil and a higher ambient temperature, I could have a not so good result, well, it's one more experience and I liked it and let's see how it will be until the end of the cycle because the next one I already ordered a starter kit from the advanced nutrients line! Let's Go!

😈 💄 🐊 🐙 💚 😈 💄 🐊 🐙 💚 😈 💄 🐊 🐙 💚 Madame Medusa is really sweet actually!! 😍 Very short! 😈 💄 🐊 Thanks @James for the goodie pack, always an honor and pleasure to grow for RoyalQueenSeeds! 🙏 💚 💨 Thanks @Green_Buzz_Nutrients for sponsoring the nutrients for this grow!! 💚 💚 💚 Amazing product line!! 🌞 🌱 If you like to try them, you can find a generous discount on their products in my setup description!! ⬇️ Thanks @MedicGrowLED 💚 I love the Light!! 🌞 ___________________________________________________________________________________________________________________ Medusa F1 Medusa is a true F1 hybrid created from pure, inbred cannabis lines. She boasts uniform grow traits, mouthwatering aromas and flavours, and plenty of potency. If you're looking to bring stable, elongated plants into your room, tent, or garden, look no further. Medusa F1: Mouthwatering Aromas, High Potency, and Mid-Size Plants Though she won't turn you to stone like the mythical goddess after which she's named, Medusa F1 is bound to get you plenty stoned in her own special way. Combining genetics from inbred lines deriving from Sugar Magnolia, a thick and sweet indica, and American Beauty, a fast, fruity hybrid loved for its well-balanced, positive high, the result is an autoflowering F1 variety that produces fresh flavours, a unique cannabinoid profile, and large yields. Aromas, Flavours, and Effects of Medusa F1: Mint, Pepper, and Fresh Fruit With a Balanced Cannabinoid Profile Medusa F1 is a very aromatic cultivar with a vibrant concentration of terpenes bearing notes of fresh mint alongside an upfront peppery kick, backed up by hints of fresh fruit, berries, and tangy fuel. Her thick, frosty flowers consistently produce high levels of THC and CBG, as well as high concentrations of myrcene, ocimene, farnesene, and caryophyllene. As a result, Medusa F1 has strong effects that relax and stone the body from head to toe (brought on by particularly high concentrations of myrcene and farnesene) while motivating the mind with an uplifting, creative, and motivating kick (thanks to high concentrations of ocimene). Growing Medusa F1: Tall Yet Manageable Plants With Heavy, Easy-to-Trim Flowers Medusa F1 seeds produce elongated autoflowering plants with long, strong branches and well-spaced internodes. Plants regularly grow up to 80–85cm tall and boast a fast flowering time of just 42–45 days (72 days from germination to harvest). Thanks to her genetics, Medusa F1 produces plenty of thick flowers with big, swollen calyxes that make for very easy trimming. Medusa F1 is very stable, handling stress and unfavourable growing conditions with ease, though she is somewhat susceptible to Botrytis, so make sure to keep tabs on the humidity in your grow room/garden. Flowering time: 40-45 days 🚀 https://www.royalqueenseeds.com/f1-hybrid-cannabis-seeds/621-medusa-f1.html _______________________________________________________________________________ SETUP: 80x80x180 cm Zelsius Medic Grow MINI SUN-2 240W Full Spectrum LED Built-in Timer LCD Display high PPFD of 2300μmol/m2/s @ 6 ’’ in height, efficacy is up to 2.8μmol/J, and PPF is up to 672μmol/s built in controller with timer and dimmer www.medicgrow.com Green Buzz Nutrients Shouts go out to my sponsors @GreenBuzzNutrients, thanks so much for your support! 💚 💚 💚 If anyone would like to try their amazing organic products, use code GD42025 for generous 25% discount (for orders of minimum 75€) ✨ https://greenbuzznutrients.com/ custom exhaust fan 320/270cm³/h Carbon Active Granulate 240cm³/h tab water pH 8 - EC 0,25 with Calmag to 0,5 Advanced Hydroponics pH minus Grow + Bloom to pH 6.2

week 5 - 1/2-1/9 1/4 heavy defoliation

They are growing healthy. They all recovered from the issues, but they are all small.. They are drinking a lot lately, I've been struggling to keep the temperature below 27/28°. The only plant who didn't had any agressive movimentation, handling, whatever, has 90 cm, huge.

6/15. Glad I listened to my intuition and held off watering. IT Rained andctheb again last night. Plants seem to be doingvreal well. Slight burn on lower leaves where it was before from being in the garage and I found on spot that LOOKED like it could've been caused by a pillar but it's right on a burn. I sefoliated one leaf. This is minor stuff. Next watering I'll be adding silica. I'm not giving nutes until the plants tell the need them. The 3 gallon ones may receive nutes first as they'll run out. I'll update as I progress. Went back over at 8:30. It's just overcast with showers. Plants seemed to like it. They all look good. Noticed a chunk out of a leaf and a POSSIBLE pillar spot. I'm going to have to get the BT out and and give the girls an application. I'm waiting until they settle in a bit more though. I need to start LST as well AND the light dep. Unfortunately "life" has made things difficult. I'll get back over there today though and I'll start the light dep. Kinda makes me wonder though. The 1pth planet I wanted to light deo is tge same size as the others in the much bigger containers. The thought came that maybe I should just hold on to them as extras in case something happens. I also am going to start silica next watering but it will be a few weeks before any nutes are given. UPDATE: WENT BACK OVER AT FIVE AND TOOK ACSHOT TON OF PICTURES. IM TRYING TO UOLOAD NOW. THINGS ARE POPPING OFF AND IM GETTING A NICE SWEET SKUNKY ODOR. WHILE I WASCTHERE WE GOT A QUICK SHOWER. DIDNT LAST MORE THAN TEN MINUTES BUT IT RAINED HARD. I FOUND A TEMPORARY SPOT TO LIGHT DEP THE TWO PURPLE PUNCH AND THE ONE TENTH PLANET. I DONT SEE ANY LIGHT LEALS AND ITS SURE AS HELL DARKER THAN IT IS AT NIGHT. I'LL SEE HOW IT GOES LEQVING THE COVER ON ALL NIGHT. IF IT DOESNT WORK I MAY JEED TO ADD A FAN OR CHANCECTHE SPACE. I ALMOST DIDNT WANT TO DO THIS AS THE 10TH PLANET IS KEEPING PACE WITH ITS SISTERS, DESPITE THE SMALLER POT. OH WELL I WANT TO GIVE IT A SHOT AND I DONT WANT MY CAGE OVER CROWDED. I'M LOOKONG TO DO A 6:30 TO 6:30 SCHEDULE. 6/16 First night of light dep for the three little ones. I won't be able to call them that soon. I'm currently trying to upload a video. My grow buddy mentioned the droopiness of a couple of my plants in this video and how I hadn't been watering. This kinda scared me so I ran over and checked the too few inches on the two that had a little droop and came out dry. I had some water already phed ready to go (I'm hoping that the ph doesn't change if it's stored a day or two) and used the gallon to moisten the dry topsoil. Looking back over my diary we have had nothing but rain. I then tried the "lift the pot method" and came to the conclusion that these bags were indeed still holding moisture. I assumed they would be considering the thunderstorms we've had. I held off on fullt watering because the weather said the next dry day is going to be Monday. Today is Friday so thats two solid days of rain. Three if you count today through the night. I concentrated my watering of the one gallon on the couple plants that appeared to droop. If it doesn't rain like they say it will I will need to water. I need to set up a decent watering schedule but I need it to hold off raining long enough to do so. UPDATE: I WENT BACK OVER AND PUT THE 3 GALS IN FOR BED AND CHECKED OUT THE REST OF THE GARDEN. I WATERED THE 3 GAL GIRLS A LITTLE MORE SINCE THEY WONT BE OUTSIDE. THE BIT OF WATER I GAVE SEEMED TO HELP LIFT SOME OF THE GIRLS. AT LEAST I THINK IT DID. THE RAIN SHOULDVE COME ALREADY. I WOULDVE WATERED MORE HAD THE BAGS NOT BEEN SO HEAVY. 6/17 Rained all night which is good. Brought the three transplants out but left them under the overhang so they. Dont get soaked. So far light depoing is going good. Well its only been two nights but we'll see. I'll update as I go. Not feeling well today. Went back over and checked on the plants and did a video. Probably won't get uploaded until tomorrow. I'm giving it a shot. It's raining in good shape. Plants looked fine when I was there. I need to get my supports and my trellis in place. Video won't upload. No suprise. I'll get it tomorrow. 6/18 Uploaded videos if they fuck up from me editing I'm gonna be pissed. Anyway it's still pouring with high winds. I added a couple supports where it looked like it needed it. I need to put my trellis up. I've been able to keep up with the light dep and those plants haven't been used and abused. They stay in the building facing the sun when the weather is this bad. I think they're looking great. I may start a new diary just for them. Sun came out around four. Trying to upload a couple videos but doesn't look like it's going to happen. Tucked the three little ones in at six. It had stopped raining and the wind died down some. If adverse conditions create good plants then I'm on the way to a great harvest. Minor bleaching and burns on a few lower fan leaves and some wrinkling on some from wind burn other than that plants seem to be acclimated. Did a video but will have to wait until tomorrow to upload 6/19 It's not raining! We got four inches this past storm lol. Having the bags raised on pollens I believe has greatly helped. As did the high winds that help dry the soil in the bag. Had to add a few temporary supports. Light dep is going good. Adjusted amd found a better spot. I'm considering getting "pool noodles" or some other thing like a Hulu hoops or pvc pipe and making either a frame on the roof for the entire cage or on top of each individual plants posts so I could roll clear plastic over it when it rains. I'm thinking about later on in the fall when this could really help. I found a roll of plastic. Also thought about putting that up on the back wall but I didn't. What I'm doing right now is working. I just need to keep up with it. I also need a solid watering schedule to stick too. It's hard when each plants needs are different. Oh well. I'm just happy to be growing again. I pray to the cannabis gods and the Lord above himself that I'm not plagued with the problems of last year. 6/20. I'm sick as fuck but I got the little girls out in the sun. Light dep is going good. Plants look great. I'll update when I feel better and if I do anything. Still feel like shit. Luckily I looked up just in time to get to the grow and put the little ones to bed. They were thirsty (especially the bushy 10th planet so I gave them about 3/4 of a gallon of water. Got lots of run off from 10th planet. Didn't notice as much from the others but I was hurrying. I'll upload later. Still sick. 6/21 Plants seem to be doing great. I removed some lower growth on a few just to see how they respond. I'm going to do some defoliation down low and I want to see how the plants respond. I'm planning my first full watering Thursday as we have a real good stretch of weather after that. Grow bags are still heavy as hell so they have sufficient water. I'll be adding silica nextcl watering. Not time for nutes but I think it would be okay to start the silica and in another week I might start small doses of nutes. The light dep on the little girls is go8ng great! It's cool I'll get to sample some of these strains before fall. I'll update after I do something. Tucked the girls in at 6. I might need to think about transplanting that 10th planet in the 3 gallon. I watered until runoff yesterday and it's light as hell today. Plus I fimked it and it's bushy as hell. I've Bern turning it si it gets all angles of the sun. I have a bag of 707 left and a 5 gallon bucket with holes drilled all over it like an air pot and a ten gallon grow bag. This will take some thought. Hot af today. I wad able to lift the grow bags so I decided tomorrow will be the girls first real watering. I think I'll be adding silica and possibly kelp me kelp you due to the heat stress I've been noticing. Nothing bad just a few tacoed leaves. I've had several people that have seen my grow in person tell me that this is by far the healthiest (not biggest I've had bigger) but by farcthe healthies5 crop I've grown. They believe I'll have my best year ever this year. I hope they are right. Vid won't be uploaded until tomorrow. 6/22 WATERED TODAY. I'm still a little gun shy with watering. That fusarium last year pretty much traumatized me. I left the container plants alone. I gave them a little water but not enough to do much. I used a pump sprayer on a mist setting and went one by one and back around gently watering the entire medium. This took forever and I'll be finding a different way to water. I hope this was enough water. I'll check later on and if they look like they want more I give it to them. I started LST on the light dep 10th planet and tucked some branches of a another under the tomato cage. I had missed that earlier. I also went around and cleaned up the bottoms on most. Getting rid of things that will amount to nothing. I noticed what looked like thrip damage on ONE leaf on ONE plant. I also saw a chunk missing from a grasshopper bite and a finger of a fan leaf petiol was broken so I'm going to need to get my ipm up and running. I'm unsure whether I'll be using chemicals or predators. Probably BT and spinosad. That's what I've had the best luck with. I also noticed bottom leaves dying off on the bottom of a couple plants. It was where the burns were though so I'm almost 100% its just related to the light burns they got when i first put them up. I'll be updating the diary as I do more stuff.

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Not much change, way over fed so dealing with that..

11/4 Week 6 Light training to get the buds in light and keep her dry. Watered 11/3 one gal @6.5PH - Fish Sh!t 5ml - Karo syrup 5ml Just keeping an eye on her 11/5 Gorgeous smell coming off her purple buds, reminds me of a taffy shop. Drooling over her Growing well got her into the canopy better. 11/7 Update and bragging! Watering/feeding day today, she loved it! She is turning so pretty! The deep purple looks like fairies stole in the night and are using the little brushes to color her leaves. He hairs stand tall! First strain I have seen with them this tall. The smell is sweet with a warmed fruit smell...for those that bake, closest I can come. Still have some questions as if this is a true Runtz. From her coloring and bud development and her leaf shaping she is a good match. Have added some close ups of my lady for anyone out there wanting to take a stab at figuring her out, any info would be welcome. 1 gal 6.5 PH - CalMag 5ml - Fish Sh!t 5ml - Bio_Bloom 5ml

Looking good smell of blood Orange!!

Siamo all'8 settimana le piante hanno sviluppato fiori ben formati,ora dobbiamo avere pazienza ed aspettare che diventino ancora più grandi,la maggior parte dei pistilli é ancora bianca e le piante ancora verdi,questo significa in grandi linee che la pianta può ancora produrre,incredibile la pianta in fondo a sinistra ha quasi toccato il soffitto,un autofiorente di eccezionali dimensioni,ancora all'inizio della fioritura

This 5 plants are absolutely gorgeous, they're supper similar to each to each other beautiful leafs and structure, looks like this lady is gonna provide us with beautiful citrussy buds!! Stay tuned everybody! Great autoflowering strain by SPLIFF SEEDS!

I thought the ladies would have another 2-3 weeks left in veg, but started to notice pistils forming within the last couple of days. So flower will most likely start to begin within the next week, I’m guessing I may see a couple oz per plant come harvest day. Not bad for first time grow & using autos, from what I’ve heard a lot of people do not recommend learning with autos due to how quickly their state can change depending on what you do with them. There are some things I wish now I would have done a bit different within the last couple of weeks, I can’t help but wonder how much bigger they’d be if I did not do any defoliation at all. But I have been combating flies bad and made the call to defoliate mainly for that reason. Started to cut back on veg foods and introduce flower foods and sugars to their diet. Humidity levels still at a steady decrease, aiming to have humidity down in the 30s come flower time. Will be going on vacation for a week soon, so fingers crossed that nothing goes wrong. Will be having a good friend of mine come by every couple of days to FaceTime me and make sure everything is handled as well as possible when the time comes.

Shes just trucking along as batman has said before all I do is water her with Ph water and that's it all the nutrients were added to the soil so shes taking care of all that by herself. Batman cant wait to see some real bud growth hoping for a nice harvest considering shes just a single plant

Day 49 of flowering, and the plants are starting to show their autumn colors—looking absolutely beautiful. The buds are developing perfectly, and the aroma is getting more intense. It’s a sweet, fruity scent with strong diesel notes that sometimes have an almost chemical edge, which I really love. 👍🏻😍🌲

this is a very easy one to grow with an enormous bag appeal true her all cycle, a must in any garden, her trichomes production is out of these world, as are them grams on them fruits, a true must for us all to grow

Floracion (Tiempo estimado 64 dias) Cuarta Semana de Floracion 29/01/2024 - 5:00hrs y 22hrs: Se hace cambio de solucion nutritiva, con parametros ajustados a la semana, Esta vez no se lavan recipientes, Se verifican parámetros básicos del agua y se mantienene constantes apesar de no lavar los recipientes, mantener cuidados específicos, el olor es mas penetrante pero gracias al filtro de carbono se mantiene fuera del indoor sin aroma, empiezan a observarse mas el crecimiento de los pistilos . Agua de osmosis: Ph 6.0, PPm 950-1200, Ec 1.9-2.3, Temperatura 18°C - 24°C Ambiente: Temperatura 19-25 °C, Humedad 65%, Ventilación 20%, 12 hrs de luz , 12 hrs de obscuridad. 30/01/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 950-1200, Ec 1.9-2.3, Temperatura 18°C - 24°C Ambiente: Temperatura 19-25 °C, Humedad 65%, Ventilación 20%, 12 hrs de luz , 12 hrs de obscuridad. 31/01/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 950-1200, Ec 1.9-2.3, Temperatura 18°C - 24°C Ambiente: Temperatura 19-25 °C, Humedad 65%, Ventilación 20%, 12 hrs de luz , 12 hrs de obscuridad. 01/02/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 950-1200, Ec 1.9-2.3, Temperatura 18°C - 24°C Ambiente: Temperatura 19-25 °C, Humedad 65%, Ventilación 20%, 12 hrs de luz , 12 hrs de obscuridad. 02/02/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 950-1200, Ec 1.9-2.3, Temperatura 18°C - 24°C Ambiente: Temperatura 19-25 °C, Humedad 65%, Ventilación 20%, 12 hrs de luz , 12 hrs de obscuridad. 03/02/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 950-1200, Ec 1.9-2.3, Temperatura 18°C - 24°C Ambiente: Temperatura 19-25 °C, Humedad 65%, Ventilación 20%, 12 hrs de luz , 12 hrs de obscuridad. 04/02/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 950-1200, Ec 1.9-2.3, Temperatura 18°C - 24°C Ambiente: Temperatura 19-25 °C, Humedad 65%, Ventilación 20%, 12 hrs de luz , 12 hrs de obscuridad.