2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Beginn Woche 9 Blüte Denke für die rechte wird es die letzte Woche sein, die andren 2 Brauchen wohl noch bißchen länger. Blütetag 60 Die 3 Damen machen sich ganz gut🥀 Vor allem die ganz links wird fast schon dunkelblau/lila😍😍 Trichome sind bei der rechten und mittleren langsam alle trüb, Ember ist noch nix zu sehen.. Mal schaun wie lange sie noch brauchen, aber schätze mal noch max. 2 Wochen.

She is 4 weeks into flower and doing awesome!!! I started seeing some discoloration on her tips so I cut the nutes by 25%. She is smelling great 👍!!! Buds are stacking and fattening up nicely!! So far this has been a great grow!

Transplant to 3g -----> LOVE TRANSPLANTING!! Gives you a chance to check the root ball, you don't want to your girls to be root bound. The right size pot for the plant is crucial for a plant to remain healthy its entire life cycle. Using a tote works great for mixing the Cocoa Coir bricks in. Transplant went well and root mass looked awesome! You don't want to see your roots wrapped around a million times as that is what is referred to as root bound. I could have transplanted sooner as there was some wrapping of roots. Hard time getting feeding under control. Each plant started to absorb its own different amount each feeding. Mid to end of the week clear signs of overwatering and again was battling the heat issue caused by the lights. Deficiencies were starting to show.

Day 57. And Mimosas are real sl@ts ..... No doubts, they all time were most picky and stupid, but big, very big ... Only two from 3. Last one is the smallest plant from all first wave. Very strange . Smoothie's are nice, grow without big hiccups, maybe a bit of a runners, but all 3 looks very similar. Purple Punch - both small, the one in best light is giving me hope that I will chop her before holidays and will make some space for the guy who will be watering them during my offs. They are overfed a bit. So no feed all week. Just water. Two biggest Mimosa's will be transplanted into bigger pots, they drink a lot, dry too quick and are sad in my mind, so I will cheer them up with bigger pots this week. Still selective defoliation, some yo-yo, so ties and no spray anymore. Smell is getting stronger, but didn't bother to try one or other. Still nothing special or super smelly yet ... In the middle of the day Mimosa's finally happy, but before lights down - theirs leaves are down too. Overdrying them constantly too, can't wait to give them better house, that type of fight I hate. Day 60. All work done ! Huge work ! All girls were cleaned, defoliated and pots toped up with BioBizz Worm Castings. Both Mimosas got new bigger pots too. Happy Growing !!!

All girls have been soaked and awaiting soil apart from the strawberry bannana which is a late comer ✌️ let's hope they are okay as I did originally have 2 of each which I had too reset and decided too do small pots instead ✌️🌱💚

The pistils are starting to brown a little so we're nearing the end, but this is not a September finish! Colas and bulking up I think she's had her last feeding. For awhile there I was worried about how this strain was going to finish but in the last couple weeks she's been really strong. I can't wait to see how she looks on harvest day!

Our White Truffle by Zamnesia is a very interesting plant, it has slightly irregular internodes, at least this one I got, there is a bit of a need to invent a different main lining than usual, but both Zamnesia and I love to take a gamble and we are sure to bring home a good result. Remember that we are growing a plant worked with the techniques indicated and one left to grow without pruning to preserve its speed, in the previous/next diary you will find the other plant of the same strain. In this diary we find the plant worked with Main Lining. We topped the second internode and maybe it was a bit too early, or we simply found a plant with slightly irregular internodes and now we are developing according to irregular brunches, I cut some off, I top some and send it to flower shortly. Slow down with nitrogen while doing this technique, you may not need everything that is recommended by the manufacturers, so if the leaves become too dark or worse, the tips of the leaves arch downwards and the leaf becomes "crunchy" we are at the moment to lighten or stop the basic grow fertilizer. . Comparison is the salt of experience, so the plant in the other diary will be worked very differently, go and see it to compare them. We have started the Plagron fertilization program, we are in 100% organic configuration, the soil is recycled Promix + 1/3 fresh soil + 10% Perlite + RQS Mycorrhizae Mix (4 g in the mix, 1 g under the small fiber pot). We are feeding // 1 ml/l Power Roots - 1 ml/l Pure Zym - 1 ml/l Sugar Royal - 2 ml/l Alga Grow We sprayed 3 ml/l Vita Race Foliare once a week. https://plagron.com/it We always have the excellent Mycorrhizae from RQS running in the soil https://www.zamnesia.io/it/5778-mix-micorrize-easy-roots.html Try this strain, it's an autumnal delicacy ---- // https://www.zamnesia.io/it/11184-zamnesia-white-truffle-semi.html Zamnesia Brief Description // The result of crossing GG and Peanut Butter Breath, White Truffle boasts a first-class lineage, taking the best of some of the best American cultivars. And as you would expect, she has a lot to offer: a high THC content, an irresistible flavour and a relaxing, carefree effect. Oh, and let's not forget that she's incredibly easy to grow too! Buy your seeds today and enjoy easy harvests of delicious, USA-grown bud. And as you would expect, she has a lot to offer: a high THC content, an irresistible flavour and a relaxing, carefree effect. Oh, and let's not forget that she's incredibly easy to grow too! Buy your seeds today and enjoy easy harvests of delicious, USA-grown bud. The whole world of growing and more is here at Zamnesia - just take a look around the site and you'll find "the best that nature has to offer" in all shapes and colours. The new strains are amazing and the old ones are no exception... -- // www.zamnesia.com

Der Stretch ist vermutlich abgeschlossen und die ersten Zuckerblätter mit schönen kristallinen Trichomen sind sichtbar. Sie entwickelt sich prächtig.💚 Absolut auf die nächsten Wochen gespannt!

No es una genética que crece mucho en altura. Quizá por el lst continuo que tuvo, pero tenía una estructura ideal

Week 5 is about finished. I started the girls on the finisher product to help. Harden the buds, basically only another week and a half before I begin the flush.

Empezamos cultivo. Semillas HUTZ de BaskTringleFarms Cruce: Zero x Gluerke 13 Semillas Regulares. Se usa tecnica de la servilleta para germinar. 13 de 13 germinadas en 2 días. Se pasan las 13 a Jiffy en un invernadero y en 3 días tenemos todas las semillas listas para trasplantar a maceta de 1L y meterlas en el armario. La lámpara LED TheJackson 250W es regulable por lo que los 2 primeros días esta al mínimo 20W. A partir del segundo día subimos a 50W. Lo mantendremos una semana. Por ahora un solo riego en el trasplante con Rhizotonic de Canna

@MephistoGenetics, Hi all the happy people here in GrowDiaries. This is my second cultivation ever and it will be fun to try a bigger space than my closet grow. First, I'm just going to say I'm done with the construction of my new growroom. The room is 2.14 meters by 1.7 meters and has a ceiling height of 2 meters. It provides a floor area of ​​3.6 square meters. I use a 54 Watt Lightwawe T5 for germination and 2 Pcs 400 Watt HPS lamps. I have a channel fan that replaces the room air about 40 times an hour to get a comfortable environment in the room, the air enters a fresh air intake from the outside. The air is purified through a carbon filter to then leave the room to the rest of the basement. Then I use that heat to heat the rest of the basement. I will use 10 pcs 15 liter Autopots to grow with and a 100 liter water tank that supplies the pots of water and nutrition. I will grow completely organically in soil and will watercure my buds to get the best possible medicine for me. But there are no cultivation rooms to be displayed here, so I continue with what is most important. I am very excited to see how the new growroom will work and how this Stilton Special will turn out. Strain Name: Stilton Special F1 Genetic heritage - Sour Livers F3 x Northern Cheese Haze F3 Strain behaviour - Stilton grows well from the off, and develops into a stout but branchy specimine that leads to a fine yield of awesome flowers. She's not too stretchy but also is sizeable enough with good growth, to train and shape to your liking. Flowers develop pretty fast and grow to a good size, it may to advised to clear out some undergrowth and if needs be a very gentle defoliation mid-way through the grow, although leaf tucking may suffice well enough.The end product is of top shelf quality and she doesn't lack in yield either.Give your garden, nose, and body a treat and be sure to indulge in a slice of Stilton this year, You won't regret it. Size - 50 - 70 cm Structure - Medium height but bushy Flower Density - 9/10 Indica/Sativa - 65/35 Cycle Time - 65 to 70 days from sprout Yield - 90 to 140 grams as a single plant Best Method for overall high yield - 9-12 per 1,2m x 1,2m sq in 10-15 Liter pots (Soil) Aroma - Very strong, Cheesey/fruity/sour/spicey/ with a dash of coffee. Taste - Dank berries Effect - Good hybrid powerful but balanced effect Medicinal Benefits - TBA Best Grown - Indoor/Greenhouse Cannabinoids - TBA Extract information - Ideal extract candidate - High in resin, oil and terps. -------------------------------------------------------------------------------------------------- 2017-09-25. Week 5 starts. Added a video of the girls. Gave the girl water and nutrients. This girl is 24 cm high and have grown 7 cm in 7 days. I flushed the girl yesterday with 40 liters of tap water. Im lowering the humidity in the room with a dehumidifier and try to have 50% air humidity. ---------------------------------------------------------------------------------------------------------------- 2017-09-29. Installation of the New light rail in the grow room. Its Advanced star light V 5.0. Added a video of it.

Very happy with this strain, fast and frosty! Only going to become a better grower by working hard everyday. Extremely pleasing smell and terpene profile.

Tag 91: Die Trichome der Cosmos F1 Automatic von Royal Queen Seeds sind schon zum großen Teil trübe. Die drei Hauptcoli sind traumhaft gefärbt. Die Blüten sind sehr dick, fest und glitzern voller Trichome. Habt einen schönen Tag. 😀

Welcome to week 8 of flower for this wild project! We will be looking to check trichomes over the coming days to see how things are coming along. I know for sure PGC#2 will be ready far before the rest but we will check all of them none the less. As we reach the race for the finish line Im very pleased with what i've accomplished with these plants this grow! Huge shout outs go to @MarsHydroLED as well as @Seedsman for their outstanding gear and genetics to work with! HUGE shout out goes to all my followers and people who stop in a like! Keep giving inspiration to grow! -The Projexx Day#50F Pictures N/A. Ladies are starting to slow down on new pistol growth and focusing on ripening up. Day#51F Watered ladies 6L each at 160PPM. We've began flushing them. Day#52F PGC#1 leaves starting to turn quite purple, Banana Krumble still has a while to go. Checking trichomes tomorrow. Day#53F Pictures N/A. Watered ladies 6L each at 160PPM. PGC#1 trichomes are doing well mostly cloudy little amber, Banana Krumble still has lots of time to go and PGC#2 is almost ready displaying lots of amber and lots of milky trichomes gonna give her a few more days. Day#54F Pictures N/A. Banana Krumble still thickening up , PGC#2 is looking quite ready and PGC#1 is far behind her sister! Day#55F Harvested PGC#2. Watered ladies 6L at 160PPM. PGC#1 is turning more purple by the day quite the lovely show! Day#56F Pictures N/A. Banana Krumble still thickening up but displaying more orange pistols , she's gonna be a 70 - 77 day girl but I'll get big rewards! PGC#1 ripening up daily! Recap: Over all things went super well this week! We harvested the smallest plant in the room and ended up with almost 500G of wet weight roughly 6-7 ounces off that plant dry, she smells very very gassy with light hints of candy and fruit very pleasing combination to say the least. We are coming up on the finish line quickly for this project Im excited to see what the other plants will yield!

Week 8 Flower Flush flush flush 💦🚽💦🚽💦 It's extremely important to flush for at least 14 days when using synthetic nutes, or any bottled nutes in my opinion. The aim is to make her cannibalize (fade) as much as possible before she's chopped. By doing this, she's using up her stored nutrients, causing the leaves to eventually die. The better the flush the cleaner the smoke 👌💨 even when in an organic set up, the plant needs to be nicely faded before being harvested. Otherwise she's still full of nutrients. I still feel I'll be able to get an even better flush when I eventually make the switch to RO water, as my tap water holds a ppm value of approx 150. It's not too much, but 0 is always better. She was taken down on Day 57 flower, after a 16 Day flush. I didn't want to take her any further as she was quite stressed being so close to the light. So to avoid getting seeds I waited until she had a beautiful fade before giving her the chop ✂️ I always take my girls down about 30mins before the light turns on so there is minimal chlorophyll production in the plant. This is to ensure the best smell, taste and overall burn after drying and curing. A subtle but very sweet-ish tropical fruit skin kind of smell to her. Her buds are super dense and covered in trichomes. I trimmed off all fan leaves and then each branch was hung in my drying box and placed in a cool area. She was dry after 6 days and ready to be dry trimmed and jarred for curing. I'll post those pics with the harvest update! Thanks for following and happy growing! 🐺