Day 120 PH 5.8 EC 0.2 PPM DLI 6h PPFD/ Water 18c Day 121 Tue PH 6.0 EC 0.2 PPM DLI 6h PPFD/ Water 18c Day 123 PH 6.0 EC 0.2 PPM DLI 6h PPFD/ Water 18c Day 125 PH 6.0 EC 0.2 PPM DLI 6h PPFD/ Water 18c

Mouth watering flavour, clean smoke, and it only gets better ! Barley a week into the cure and I'm already craving more of this strain Updated True Wattage, and fixed square footage of tent harvest was easy, could of gotten more yeil but she flowered early from some LST, would let this girl grow naturally in the future Buds turned out nice and chunky lots of sugar leaves just packed with sticky trichs She pressed out alright, could try a again but .4 return on 3.5 grams of bud gives 14-15% return on the first press of this strain none the less this girl puts out some wicked flavors can't wait till shes fully cured in a month :)

ALRIGHTY THEN REMINDER I DO 2 UPDATES PER WEEK 👉WEEKLYROUNDUP👈👉MIDWEEKLY UPDATE👈 We just hit week 11 and all is well , have to admit shes just killing it on the resin development 😃 ......the frost is insane 👹...... 50 days into flowering 👉 her baby Sister and it's coming along very well , I did little defolation last week 😏 shes is about 9 weeks now and looks great...👈 she's also killing it 💀 👉NutriNPK NUTRIENTS USED FOR FEEDING👈 Lights being readjusted and chart updated .........👍 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉IF ANYONE IS LOOKING FOR A PLACE TO HANGOUT VIA GROWDIARIES AND TALK GROWING AND JUST CHILL AND WHATEVER .....👈 👉I CREATED GROWDIARIES DISCORD SERVER !!!!!!!!!!!👈 LINK IS 👉 https://discord.gg/zQmTHkbejs AND SEE HOW IT PLAYS OUT !!!!!!!

01/04/2022- Hello and welcome all been away on some family matter. So today was the last day this girl got watered and will also be the last day she got light as well, she will be cut down Friday morning. The smell has gotten a lot more stronger since flush was started last week Wednesday. Will update before I chop her down.

This Permanent Marker is a smaller plant, only about 4 inches tall. But what it lacks in size, it makes up for in those super distinct wide leaves that make it a standout. While it's a steady grower, it does have a big appetite, always seeming to need more nutrients. The other Permanent Marker plant is also around 4 inches, but it's growing much faster than the other one. Its super skinny, pointy leaves are what really make it stand out from the rest. At 1st I thought this plant was going to be a dud but is starting to out pace the other plant. Happy late turkey day grow diaries world!!!! It's now day 24 from seed for all the seedlings I have going. The past few days the plants have really started to take off. There roots have really started to dig in. Every other water I am still giving 2ml per gal of fox farm grow big. I keep having to tell myself not to add any more nutes other than that. I don't want to fry my plants but I also want they to start jumping. I'm quite excited for the coming week, I think the next 7 days or so is when the plant really starts to transform and look more like a pot plant than just some random little green thing. A few days ago I decided to move the plants from under my 55w cheep amazon light to under my spider farmer 100w newer version light with the good leds in it. The plants will hopefully be ready to up pot them here soon and get them in the large tent. Sometimes when growing multiple different strains it can be really hard to balance giving to much or to little light. You don't want the faster growing plants to get to stretched out waiting on the slower plants to catch up I spend Thanksgiving day morning transplanting the plants into 2gal pots. I am useing spme promix potting soil it's my 1st time tring this soil. It is actually doing really well the roots were all white and nice looking. I think next time I'm going to add some worm casting, kelp, granular mycos, and a little bit extra perlite. I just didn't have the money with christmas coming to splurge for all the extra stuff. I got rid of 2 plants 1 of the bubble og and 1 skunk apple runtz. There is only room for 10 pots in the 2x4 tent. I will peob only bring 8 of them to flower, always good to have back ups. Yesterday I also stuck the plants back under the 500w medicgrow mini sun 2 in my 2x4 tent. They did great under it the 2nd half the day I was happy they didn't get stressed. I had put them under that light a little early about 10 days ago or so. I am expecting in a week these plants will ge going crazy with growth

Fast schon ende der 4. Woche aber jetzt erstmal Woche 3 eintragen. EC ca. auf 1.1 Am Anfang der Woche 3 in 19 l fabric pot umgetopft. Ab Dienstag 4 Tage unterwegs daher die Schläuche für Blumat...hat ganz gut funktioniert mit Umwälzpumpe im Tank plus dass "Jemand" täglich PH korrigiert hat. Video ist ca. 19.10. bis 26.10. plus bissl vom 27.10 Schon mal vorab... Samstag bis Mittwoch (Woche 4) auch unterwegs...nur das es "Jemand" nicht gab und Blumat auch noch verstopfte...Pflanze hat gelitten aber Spoiler muss sein...die Frau mit dem fetten A.... wurde geretet. Aptus schein für Blumat ungeeignet zu sein...mit TA gabs weniger Probleme bei einem älteren grow...Aptus bleibt aber zunächst mein Favorit.

One of ap colas fell and bent overnight due to the weight on her stem lol. Fixed the problem by using some tape and a support beam for her. Other than that they r both starting to stink up a lil bit and are growing healthy. Completely flushed them at the beginning of the week to wash last week's nutes out for fear of rootrot. Will give them nutes for there next feeding.

Loved growing this unknown Indica Strain the effects are medicinal, the experience is euphoric and pleasant being surrounded by flowery herby scents while you bliss out. A true gem and pleasure to grow

The one plant of STRAWBERRY SOUR DIESEL harvested 49,3 gram of EXCELLENT dried bud. That makes her the second lowest yielder of my tent. The buds are GLISTENING with trichomes and the smell is sooooooo sweet snd soooooo delicious, she is mouthwatering! My plant grew tall with long internode distances, so I had to super-crop her in order to control her height. She grew healthy all thru the cycle and had developed very nicely.

26.1.25: I have had a mistake 😕 i spilt some Fish Mix as I was experimenting with a syphon from some aquarium tubing I have. Unfortunately, I forgot to take the syphon out! Ugh 😣 However, it is fish emulsion and very thick consistency. Therefore, I was able to scrape it up with 2 of my business cards up off the counter. So added this extra into the next 2 ltr solution I mixed. All plants were watered with at least 1ltr of dechlorinated water PH'd to 6.3. Containing the following nutrients;- ♡ 3ml Seachem Seaweed ♡ 4ml Biobizz Fish Mix ♡ 4ml Biobizz Bio-Grow ♡ 3ml Biobizz Bloom ♡ 2ml Xpert Nutrients Bloom Booster ♡ 2ml Xpert Nutrients Cal-Mag ♡ .25g Ecothrive Biosys. ♡ 2.5g of Bicarbonate of Soda as PH UP. Unfortunately I have broken my PH pen too. Accidentally submerged it under water, then the readings went crazy. I'm leaving it to dry by standing it up but I'm most likely going to need a new one, which is extremely annoying 😑 I'm going start implementing the Scrog net probably some point next week if I let the plants that are in flower to just grow up now, as I do believe that I stunted Watermelon 🍉 by using too strenuous LST. Hopefully the plants still are able to grow upwards at this stage during flower for all the Purple Punch P1, P2 and P3. We will soon see! 28.1.25: My PH pen has packed up 😣 I've got to wait to get another. It's super annoying 😑 so I'm just judging by memory how to regulate it. Unfortunately, that's my only choice! I'm also out of the dechlorinator 😕 things are not going very well this week so far 😅 I guess we will have to make do, until I can get replenished. 29.1.25: I removed all the plants, hoovered, and disinfected the tent. There were several accidents when watering, causing some debris and splashes. I decided to just go ahead and get a few other things done. It's crazy how quickly things can become neglected! 😣 So I defoliated moderately on all plants except Gorilla Cookies seedling. I have absolutely dropped the ball on my lighting situation! My light was much further away than I thought! I turned it up to 100% and dropped it down to just under 2 feet away. After I reset the tent, I set up my scrog net. I removed 80% of all LST pegs. I am planning on letting the scrog net take over the training from here. I really want to minimise the risk of mould, so I decided to start using my dehumidifier. It has raised up to 73% RH this week, so it's necessary. Hopefully, I will be able to get my dechlorinator and PH pen soon! That's kind of essential to me. It's been a little bit of a stressful day in the garden, to be honest 😅 30.1.25: So I am expecting the arrival of my new PH pen today, as well as my new bottle of Ecothrive neutralise. It is supposed to measure the TDS and EC in addition to the standard TEMP and PH, which I had previously. Obviously, time will tell if it's any good or not. I realise, much too late now, that my lighting is very insufficient for this many plants. I need double the watts at least, and coverage is very poor. I remembered I had an old LED floor lamp, which was in a corner being unused. I decided to take the stand off, and Zip tied the pole onto the tent pole at the back left corner. I have no idea what the wattage is, and it's quite pathetic 😅 but I mean, I might as well use it, being as I definitely cannot afford more lights! 😣 Next grow, I will probably only grow 2 plants, max 4, and just use larger 7-10 Gallon pots. Being as I ran out of dechlorinator, I only watered some plants properly because I only had enough to make 6 litres. When my pen arrives, I will get everything properly watered. 31.1.25: The leaves of several plants are showing signs of iron deficiency. This is likely due to my PH pen packing up earlier. I now have a new pen. Due to this, I decided to use my Seachem Prime, which I use for my aquariums. As I wanted to get them watered properly, and my Ecothrive Neutralise hasn't arrived yet. I watered each plant with 1ltr of dechlorinated water PH'd to 6.0. I added .25g Ecothrive Biosys to the water. May give them a breather on the nutes and just use the Cal-Mag and Biosys. Being as I've just topped dressed with rich fertilisers, I'm sure that will be OK for the next several waterings. Bubble Kush

Week 20/5 - 26/5

9/21 Wont allow video uploads so this is it 9/22 pic Still in veg starting week 4 which is a good sign for a good harvest though she remains smallish not sure why lots of light good nuets so genetics 9/23 Vids up Still no sign of flowering solid veg whish is highly promising. Some hairs though, so debating intro of small dose of bloom 1ml/g but prob not today Increasing Grow nuets to 7.5/gal tonight each of A&B. 9/25 Still in veg looking real good 9/26 Hit 15 inches still in veg looks like we got a good grow Vid/pic slow ride... 9/27 Hit 19" and still in veg

🤩☮️

todo va bien, esta semana han crecido, pero creo que se han detenido casi por completo, hice una ligera defoliación, intenté limpiar las partes inferiores debajo de la red, dejando las hojas que aún están verdes. nos vemos la semana que viene buen cultivo a todos MrBigBong 🍁🍁💪👽👍

11.01 - Watered ~ 3L with worm casting tea (2Gal water, 2 cups worm castings, 2 TBPS Roots Bloom Terp Tea) 11.02 - Solid defoliation of fan leaves and removal of bud sites below scrog 11.04 - Misted soil 11.05 - 3 L w/ 1/2 tsp Recharge - Top dressed with 2 Tbps Terp Tea Bloom

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

will be back with this strains im ok with No air conditioner!!! dry : 60G it ok i will play this with strain’s again

Just feed water ph to 6.00 area.

The 3 amigos doing well.My 3 baby sharks starting to grow teeth lol.Very uniform growing to each other.Smells are coming through.Happy plants.They loving the lst

The plant is drinking over 3 gallons of water every day. I’ve lowered the PPM’s to 600. Everything seems to be ok now. I will try to change the reservoir water every 5 days from now on. She started to smell amazing! Very lemony 🍋 P.S. Lowerd the TDS to 550 PPM on day 46. Also just realized that I stressed my plan with to much light. I moved the light at 13” and dimmed to 95% ... I will continue to dim it 2% every day...