_____ Week 11 | Day 78 - 84 | 4th week of Flower ______ Day 78 & 79🌞 - What should I write... Not much has happened, I leave the plants alone. - I put my dehumidifier in the tent this week, it starts as soon as the LF rises above 60%. At the moment it is winter with me and the humidity is very low anyway, saves energy Day 80 🌞💧 - each plant 3 liter Day 83 🌞💧 - at the end of week 4 I provided them with nutrients again. They have become thirstier and now drink 3 liters every 3 days ____________________________________________________________________________________________ Light - 12/12 h - 560 Watt - 2x 200 Watt Toplight - 4 x 40 Watt Lightbars PPFD - 900 - 1000 µmol Temp. avg. - 24,4° Hum. avg. - 58 % RLH

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

2 more weeks and i think she is done

Plants are doing well. I'm going to top them soon. I tend to wait on topping to reduce stress, you never want to remove more that 1/3 of the plant( to each their own). I'll be FIMing the Sensi Star and Topping the Peyote. Pretty sure I stunted the White Widow from exposing it to the cold snap we had back in late February. I will top it and try and clone off the topping and see if that clone takes off. In my past I cloned plants outside in Mid June back in 2012 on 5 foot Agent Oranges, those clones caught up to the plants outside and eventually outperformed them, in less than two months. Never would have found that out if I didn't try. Happy growing everyone!

RO flush time..... You k-no what that means wooot! 3 Gal, H2O

So I am entering week 2 of the 12/12 flip, the ladys are showing sex so hopefully later in the week I will get actual pistils so I can start counting down flower days. Day 9 seen pistils but I had to perform a major defoliation as under the canopy was bad and I didnt want powdery mildew due to leafs sweating ect. Day 10 24 hours after defoliation, you could not tell I done a thing to them, im hoping its just a odd leaf now and then thru the rest of the flowering period. Any advice is welcome guys 🙏 I will be adding more fans for airflow. Day 12 of the flip and the girls are on there way to becoming ladys lol I think there's going to be a few nice kolas in there when done, ordered the new box fan for the floor to create more airflow to reduce the risk of pm and mold. I need to top up soil as roots are showing so under the net I go again. Wish me luck.

Day 37 - Everything looks good.... they are drinking so much.... i built a bit more stable light setup, and i'll need to buy afew more plugs and stuff, to fill it out ...... i organised all the 2700k globes, ready to swap in..... still waiting on the exhaust fan, so i'll keep em in "grow" mode another week or 2 .... i'm vaping a leaf or 2, and drinking "green tea hot chocolates" .... it is so delicious in hot chocolate .... my health has picked up since starting the vape/drinks... the 21 thc/19 cbd is supa healthy ... Day 38 - Getting them ready for Flower, noticed they are lacking in nutrients, so i started em on the heavier dutch master gold flower nutrients. Day 40 - Added some pics. (they look droopy because they in night mode, just turned on lights for pic) - Started them on 12/12 flower, still working on getting all the lights, gear, and globes, for the right spectrum and amount of light.... atm got - 7x 2700k (click) , 2x 6400k (osram), + 60w light panel .... i'll go get some more gear today and hope to expand it to 10x 2700k , 2x 6400k ...... I put the exhaust filter fan on seperate power, so it doesn't go on/off with the timer, but stays on all the time.... it totally fixed up any odour problems, haha ....... Added some different pics for my light spectrum ... etc..... the outside winter temp is now at 40-60oF (5-15oC) so with the 12/12h, the temp inside the tent is dropping to 60-80oF (18-22oC), abit low, but flowering is supposed to be in winter, so idk, see how it goes..... I'm new to growing, so idk how this will work out....... just working it as i go.... i noticed afew spots on a leaf, so i upped the neuts abit more, they are still drinking like fish, huge amounts.... Day 40 late - new vids - Got the extra gear and installed it all... redesigned the initial setup, refined it with proper measurements, had to get my steel rule out, lol....... got the 12 leds in and running, 10x 2700k clicks, and 2x 6400k osrams, with the panel..... it's lookin beautiful and got the exhaust on 24 hrs now, all working perfect with 12/12 timer.... they are forming a few spots on leaves, so hammering the nute water into them now... they are drinkin 1-2 litre of nute water a day each.... (nute water = Dutch mater gold one grow/flower, 800-1000 ppm 6 PH) 😎 Some details about lights.... i did some maths on it, and included some handy pics..... because i'm using cheap leds, i'm keeping the distance at 25cm, they are leds so they won't burn, and it massively increases the lumens/lux/Par, and with my home built light setup, the leds are spread out enough to keep it lower.... worked out good..... something of note is that all the led globes are built/made in china... however different marketing/selling companies from around the world... and the german osrams are 1w leds ... by far the most superior, but anyone can design and send the requirements to china to build em, haha) it's not a contest, we all work together.... haha.... the person with the best led light globes gets my business, because i want the best for my babies.... :) (let me explain- 1w led vs 0.5 w led, the watt of the led projects the light, lumens, LX/PAR etc much further the higher the watt of the led....my 600w panel has 0.5w leds, the philips has 0.75, the osrams have 1w leds..... ) each globe philips (dutch)- 10.5w internal - 14 leds 0.75w 1055 lumens each globe osram (german)- 10.5w internal - 10 leds 1w 1080 lumens each globe click (cheap)- 9w internal - 16 leds 0.56w 800 lumens light panel - 70w - 600w 30 red - 8 red 12 blue - 8 white 7500k 1 ir - 1 uv = 20x blue 6400k - 38x red 2700k internal - 60 double leds - 120x0.5w 6000 lm

My first time making alcohol tintcure Using the c1 , Hold on tight gonna be a trip

The cultivation was very easy and relaxed. I just watched the insanely fast growth and did LTS. 😄 But by my mistake, the plants received a small nutrient burn, at the moment when the plants were at the end of the peak of maturation. This did not do much damage to the plants, but after that the plants were watered with clean water and washed with FlashClean. The inflorescences are dense and have a large number of trichome crystals 👌

Ce run ce passe vraiment a merveille,aucun souci, plante vigoureuses et très saine ! Rien a signaler rien a dire ! Juste parfait ! Merci kannabia, Madame grow , et trolmaster !!! 🚫 Update, après une inspection hebdomadaire je viens de déceler 2 pied herma , que j'ai éliminé directement, plus que 5 plantes , j'espère que sa va allez 🤞 3 nouvelle hermaphrodite détecter, certaines avec des cloches, d'autre des banane plus subtil a détecté, un totale de 5 herma sur 7 , j'ai jeté les herma, grosse perte de temps et d'argent 😵

10/10

Everything is going fine so far. And the smell is just amazing and the trichomes are growing good and allot. By the looks of it its all going how it supposed to grow. I started this week with lowering the nutrients from 4 ml to 3 ml. Upcoming week I will start to flush till harvest time. Oh yeah can't wait to taste and get high from my own grow. 😍😀

Comienzo de la cuarta semana. Va muy bien, le hice más LST y la fumigué con antihongos.

The greatest diary continues.. Dogs on dogs on dogs

good !

Weather in my area have been getting my tent in the low to mid 80’s I’m going start running my ac in my house to keep the temps down somewhat going add his green bloom tomorrow for day 23 of flower other than that both looks good to me

Hi, topman! This report is about bending the "@" plant into a shape. A week has passed since the transplant and during this time a 35% flush was carried out, form laying, burr removal, but nothing was done to add stress! Look & rejoice!

So far so good. The girls look strong, have been going well. Depending on how fast the keep growing I may have to switch to a 1 gal pot sooner than I thought.

Defoliated 2 daya ago. Very happy with the grow, best scrog to date!