Jetzt ist mir klar warum Sativa so lange brauchen - nach 2 Wochen und 2 Tagen Stretch kaum noch richtige Blüten sichtbar - heute 4.1 Liter Getränk eher inhaliert als getrunken. Weiter gehts.

She is looking nice and frosty. A touch nutrient deprived— so I gave some dry amendments. I was out of town this week, but know there were a few very hot days! She’s doing great!

🌱135 giorni dal seme 🌻56 fioritura 💧 2.2 l / 48h ph 6.3 02.03.2022 Manca 1 settimana al raccolto, devo anticipare il raccolto per colpa dei parassiti, non riesco più a tenerli sotto controllo solo defogliando. Vedo comunque i tricomi lattiginosi quindi il momemto ideale della raccolta nin sarebbe troppo lontano. Finirò questa settimana annaffiando solo con acqua, e il prossimo fine settimana lascierò 30 ore di buio prima del raccolto. Aggiornamenti con il raccolto ragazzi👌🏻🙏💚

Hallo liebe Growfreunde 💞 Ich bin zurück mit dem neuen Wochenbericht 🤗 Danke an die neuen Follower, ich hoffe es macht euch Spaß meinem Grow zu Folgen An alle anderen, für immer aktuelle Updates auch Folgen😁😁😁🤗🤗🤗 So jetzt zum Wochenbericht. Alles läuft sehr entspannt, kaum was zu tun, aber es ist einiges passiert🤩🤩. Die Buds werden fetter, der Geruch ist einfach himmlisch und die Farben sehen toll aus. Leider kann man die Farben nicht so gut erkennen auf den Bildern, aber es purplet ganz schön🤗😁 Die Sweet ZZ erholt sich von ihrer Calmag Blockade und die neuen Blätter zeigen keine Anzeichen mehr auf Mängel. Die Gorilla Zkittlez holt an Größe auf und ist jetzt etwa so groß wie die Sweet ZZ, aber mit deutlich weniger Nodien. Ich denke die Peyote Critical wird als erstes reif sein, sie wirkt am weitesten entwickelt und auch die Blütezeit ist die kürzeste von meinem Setup. Danach wahrscheinlich die Sweet ZZ und evtl. Die Green Gelato. Die Runtz und die Lemon Shining Silver Haze werden am längsten brauchen. Ne quatsch, die Gorilla Zkittlez wird am längsten brauchen da sie erst seid etwa 2 Wochen blüht. So das wars für diese Woche meine lieben💞 Ich hoffe euchvgefällt was ihr seht und lasst likes und Kommentare da🤗🤗🤗 Tut gutes💞 Cioaiii👋👋👋

These girls really want to grow. Super strong and healthy!

Update beginning week 8, last week she's was showing some brunts tips, backed off nutrients to 1 times this week follow-up by plain water, she's still packing on real good tho, each day her buds get heavier as her branches are swaying when push on them!

Day 7 - Everything seems to be going ok they are all growing about the same pace! Day 8 - They are all growing pretty equal right now , extremely happy with how things are going!✌️🏼🌱 Day 11 - one plant has some twisted leaves hopefully it’s nothing serious😫 will be keeping a eye on her, other than that they all seem to be growing great! Thanks for following & happy growing friends!✌️🏼🌱 Day 13 - have a few with some weird leaves & growing a little slow, other 3 are growing pretty good! Hopefully they all do alright, fingers crossed! Happy growing friends!

Diese Woche brachte sehr viel Regen, stehende Luft und hohe Luftfeuchtigkeit. Um die Schimmelgefahr zu minmieren habe ich einen Ventilator in das Folienzelt gehängt. Hoffentlich wirkt er. Wäre schade wenn die Bubatz vom Schimmel betroffen würde. Ansonsten verdicken und verfestigen sich ihre Buds, der Leittrieb wird immer dicker. Der Test der selbstangesetzten Jauchen geht in eine weitere Woche, Demnächst kommt noch Beinwelljauche hinzu. Ein paar Blätter wurden entfernt. Vielen Dank für euren Besuch mit besten Wünschen für eine weitere erfolgreiche Grow-Woche! Aber um es klar zu sagen, bei dem Wetter, das zur Zeit hier herrscht, ist ein erfolgreiches Anbauen der Auto Candy Bubatz XL in den letzten Blütenwochen im Freien ohne Regenschutz nicht möglich. --- This week brought a lot of rain, stagnant air, and high humidity. To minimize the risk of mold, I hung a fan in the foil tent. Hopefully it will work. It would be a shame if the Bubatz were affected by mold. Otherwise, their buds are thickening and hardening, and the main shoot is getting thicker and thicker. The test of the homemade manure will continue for another week, and comfrey manure will be added soon. A few leaves were removed. Thank you for visiting, and best wishes for another successful week of growing! But to be clear, with the weather we are currently experiencing here, it is not possible to successfully grow Auto Candy Bubatz XL outdoors in the last weeks of flowering without rain protection.

Week 4 of flower complete for these very hairy Garlic Cookies by 34streetseedco. Getting very frosty and sticky 🤗 I am in ❤️ with the smells and terps coming from this plant, like walking through a pine forest 🌲 No issues this far! Did a little defoliation of the lower canopy again, more of a clean up. Also removed a few that were blocking good bud sites. Canopy is looking 🔥 I’m very pleased with the amount of stretch and evenness. All 4 plants were popped from seed, from the same pack, and are looking almost identical 👯‍♀️👯‍♀️Great Genetics go a long way 🤓

Day 36 flower had ph drop to 5.2 fix back to 6.2-6.4 looking good happy finally geting some dryer days now to so my humidity is dropping down Day 37 switch out the dense nut and added reg nuts sad this will be the last feed the girl gets In 12 days all do the dense again Day 41 plant is drinking and eating good again all is looking good in the room moved some brunches around to get more spacing ppm 400 ph 6.4

Olor terroso característico de las orillas creo le falta menos de 1 semana para cosechar

Taking the top fan leaves so the sides can catch up to the main stem. Will repot next week.

Ya vamos viendo bonitas flores 🌼

Week 15 - Flower Week 11 Lemon Jeffrey by IRIE Genetics (14 Starts for 8 Finishers) 63-70 Days Flower (9 - 10 Weeks) Weeks Observations: - Our light leak on July 6th through July 31 ‘cost’ us ~ 21 Days. Extending Harvest to account for 21 day light leak. New Harvest Date 12 Sep 2023, Plant Day 121, Flower Day 86 Seeds Wet: Monday 14 May 2023 Harvest Date Range: [ 2023-08-11, 2024-08-21] Start of week: - Dates: [2023-08-27: LJ2Q 106:F:11:1, 
 2023-09-03: LJ2Q 112:F:11:7 ] End of Week: - Plant Height Range: [46, in] - Water/Day/Plant: [935, ml] EnvironmentalCurrent: - Scotoperiod: [ 11, hrs] # 1900 - 0600 + 24 - LightIntensityPeak: [ 1000, µMol/m2/s ] - DailyLightIntegral: [ 42.1, Mol/m2] # Target: < [ 40, Mol/m2/day] - LightDistance: [ 6, in] # 3.5” LESS than Optimal for SP3000 - TempAvg: [79.1 , °F] - RH: [ 68, %] - VPD: [ 1.10, kPa] - 230*4*11/8 - FeedwaterPerPlantPerDay: [ 1265, ml] - Feedwater: [ 10.12, l] - EC: [ 2.0, mS] Environment NOTES: - RULE - CLOSE Room Airflow during LOW TEMP periods(2030 - 0700) - OPEN during HIGH TEMP Period (0700 - 2030) SOP: - WEAR CLEAN GLOVES FOR ALL SEED OPERATIONS - CLEAN All Instruments before use - Light Strengthvegetation := 100 + (#nodes * 100) - Light Strengthflower := [ 1000, 600, µMol/m2/s] - Scotoperiodvegetation := [ 1300, 1900, 6, hr] - Scotoperiodflower := [ 0600-1900, 13, hr] Environment - Late Flower (Week 10-14) (F5-F10) - Temp: 78-80°F - VPD: 1.2 ± 0.2 - Photoperiod: 11/13 - Light Strength: [930, 850, µMol/m2/s] - EC: 2.3 # Week F5-F8 - EC: 1.8 # Week F9 - EC: 1.1 # Week F10 - Finish (Week 14) ( F10) - Temp: 78°F - VPD: 1.3 ± 0.2 PPFD: 750 peak - Resin Bloom ONLY - EC: 0.8 _______________________ Base Application for 10 Gal: - [x] Primer A: [ 100, ml] - [x] Primer B: [ 100, ml] - [x] Silica Skin: [ 35, mll] # Reduce to reduce/eliminate Sediment - [x] SLF-100 (Enzymatic Root Cleaner): [ 50, ml] - [x] King Crab (Biology): [ 5, ml] __________________________________________ - @ EC: 1.7 Supplement: [ Application Rate], [ Total Delivery for 2 Gal Solution] - [ ] Primer A: [ 10, ml, gal], [ 12.5, ml] - [ ] Primer B: [ 10, ml, gal], [ 12.5, ml] - [ ] Silica Skin: [ 4, ml, gal], [ 5, ml] - [ ] CalMag Fuel: [ 4, ml, gal], [ 5, ml] - [ ] Peak Bloom: [ 4, ml, gal], [ 5, ml] - [ ] Resin Bloom: [ 4, ml, gal], [ 5, ml] - [ ] SLF-100: [ 5, ml, gal], [ 10, ml] - [ ] King Crab: [ 0.5, ml, gal], [ 1, ml] - [ ] EC: [ 1.7, mS] 85 ml/plant X 11, : 935 ml/plant/day, 2.4x of field capacity Meaning ALL Fertigation through pot is Refreshed 2.4 times per day with this schedule. ____________________________________ Mon 28 Aug 2023 # End of Harvest ORIGINAL Window , before discovering light leak LJ2Q 106:F:11:1 - [x] Will scope trichomes by hand, and set a chop date today. - Some are ready today, Some look to need 10 - 14 days. This has been an interesting grow - the extra stretch munged the SOG Strategy - with severe Chiropractics I still have >±12” of height per plant. We should be harvesting today . . . - We could conceivably take the grow as far as Day 139, if we calculate a 4 week light leak forward. That would make LATEST DATE 24 SEPTEMBER - - IF We go more than this week - Week 15 Nutes (Mixed Daily) - Mon - Wed: ONE SHOT, EC: 1.7 - Thus - Sun: ONE SHOT, EC: 1.4 - NEXT WEEK - Mon: Resin Bloom: EC: 1.1 - Tuesday - Harvest: Resin Bloom: EC: 0.8 - ELSE if we take them down next Sunday: - [ ] Been feeding The Rooted Leaf Hydro schedule, Base Nutes plus Silicon. Need to move to harvest nutes, potassium (+Resin Bloom/+Peak Bloom) at - [ ] Monday:+Resin Bloom/+Peak Bloom: EC: 1.7 - [ ] Tuesday: EC: 1.4, (+Resin Bloom) - [ ] Wednesday: EC: 1.1, (+Resin Bloom) - [ ] Thursday EC: 0.8 (Resin Bloom Only) - [ ] Friday EC: 0.8 (Resin Bloom Only) - [ ] Saturday EC: 0.8 (Resin Bloom Only) - [ ] Sunday - Harvest Growtek Mod - 170 ml/event per emitter -> 2 Gallons Input - ___ EC: [ 2.0, mS] Tue 29 Aug 2023 LJ2Q 107:F:11:2 EC 1.6+ - Defoliated (final) blocking and unviable fans. Looks better - clearer airflow Wed 30 Aug 2023 LJ2Q 108:F:11:3 EC: 1.6 For 2 Gal (ml’s) RLA Minerals 14.0 Primer A 14.0 Primer B 7.0 Silica Skin 10.5 Peak Bloom South Cascade Organics - [x] SLF-100: [ 5, ml, gal], [ 10, ml] ___ Plant Success (Beneficial Bacteria, Crab ‘Frass’) - [ ] Sunday - Harvest? - [x] King Crab: [ 0.5, ml, gal], [ 1, ml] Thu 31 Aug 2023 LJ2Q 109:F:11:4 EC 1.4 For 2 Gal (ml’s) RLA Minerals 12.3 Primer A 12.3 Primer B 9.2 Peak Bloom Required 1 qt to bring down from 1.6 to 1.4 Friday 01 Sep Jul 2023 LJ2Q 110:F:11:5 EC 1.1 For 2 Gal (ml’s) RLA Minerals 7.6 Primer A 7.6 Primer B 5.7 Peak Bloom Sat 02 Sep 2023 LJ2Q 111:F:11:6 EC: 1.4 Peak Bloom as Needed for Dilution - [ ] Scope Trichomes Last Time With a 21 day light leak, we’ll extend harvest to Day 118, Flower Day 90 - or 69 Unleaked Light Days . . . Through End of Harvest, Daily Daily Feed through Harvest Day -2 Per TWO Gallons: EC 1.4 - [x] Primer A: [ 12, ml, gal] - [x] Primer B: [ 12, ml, gal] - [x] CalMag Fuel: [ 4, ml, gal] - [x] Silica Skin: [ 4, ml, gal] - [x] Peak Bloom: [ 4, ml, gal] - [x] Resin Bloom: [ 8, ml, gal] - [x] King Crab: [ 0.5, ml, gal] - [ ] SLF-100: [ 5, ml, gal] - [ ] Reduce to EC 1.1 on Harvest Day -2 - [ ] Reduce to Resin Bloom Only, EC: 0.8 Pre Harvest Day DECISION - We’ll take the girls down Flower Day 83, Nominal Day 62 assuming 21 Day push for light leak. It’s not in practice3 that long - we should be good, if not - latest day 90 (Nominal 69) ____ Sun 03 Sep 2023 # Flower Day 77 LJ2Q 112:F:11:7 EC 1.4 - [x] Dismantle And Clean Res, Pump, Chiller, Lines - [x] Clean Res - [x] Clean Pump - [x] Load Res with a a gallon of HOT tap water, a cup of 30% Vinegar - [x] Run Pump for :30 - [x] After 30: Reverse Lines - [x] Run Pump for :30 - [x] Stop pump - [x] remove tubing from pump and chiller - [x] Remove pump, reclean - [x] Clean Chiller Tubes - [x] Rinse with 30% Vinegar - [x] When Dry: Reassemble & Replace

Contando ya los días.. Prácticamente está todo hecho y sólo queda esperar. Ya sólo estoy regando con agua pura con el ph regulado. Estoy haciendo un lavado de raíces de dos semanas ya que busco el sabor lo más limpio posible. El clima del exterior está ayudando mucho y he podido mantener la humedad en los parámetros correctos. Espero cortarlas sobre el finde que viene Muy contento con lo que veo, la calidad promete 🤩

May 3- I picked up my clones for this season today. Plants appear very healthy and are currently under two 150 Watt HPS until these plants can survive the cold Maine temps which won't be for a few weeks. From here the will go in fabric pots ranging from 30 to 50 gallons and trellised. The soil mixture that I use is 1/3 happy frog 1/3 FFOF and 1/3 roots organic. When I get good weather I need to clean up last year's mess and wash my bags. I couldn't take pictures due to low memory but I will. I'm going to start hardening the plants off a few hours each day. 5/9 Watered everything and started LSTing but I need more room. Weather is going to be great so I think I'll hold off on training others. I pinched a few and I'm going to try to get the plants out side some so they can harden off. Planning on dumping soil and washing bags today. I got most of the cleaning done. Bags are dumped and soaking. Soil needs have been established and I'm planning on either ordering from Amazon or picking up in person the next couple days. Plants never made it outside today as it was rather windy.

Day 7 - Everything seems to be going ok they are all growing about the same pace! Day 8 - They are all growing pretty equal right now , extremely happy with how things are going!✌️🏼🌱 Day 11 - one plant has some twisted leaves hopefully it’s nothing serious😫 will be keeping a eye on her, other than that they all seem to be growing great! Thanks for following & happy growing friends!✌️🏼🌱 Day 13 - have a few with some weird leaves & growing a little slow, other 3 are growing pretty good! Hopefully they all do alright, fingers crossed! Happy growing friends!

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

Hello! 32 sprouts feel great! In 2 weeks I will choose the 16 best plants. and transfer them to flowering mode. The fight for a place in the grow tent begins! Only the strongest will remain!