Felt greedy and moved SBR back, they're together again, started one more plant, planning to add another diary soon. Girls look good! Already started blooming, stigmas appeared at the beginning of week 2, buds seem to start forming. Since SBR is a bit smaller than KE she has a podium to stand on. Like a queen ahah. I've cut 2 lower branches of KE, and few from SBR. Not sure about defoliation at the moment, Leaves under the lights seem to be ventilated well and aren't wet

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hey everyone! It's week 2 of flower and this week was a surprise! The girl on the back, the one that has the "long" stem leaves and I didn't have high hopes started to grow a lot and develop quite fast! I'm quite impressed by her! Flowers are also starting to be way more developed this week, which is absolutely amazing I'd say and looks very pretty! This week was very uneventful besides that! And since they are growing so well I decided to increase the amount of nutrients! 😍 I also trimmed a lot of leaves from both babies speciallyy the one from the back) and played with the LST a bit more. Both of them were quite bushy (specially the one in the back) and I hope this won't affect them negatively! Probably not but we never know. Anyway, that's all for now! I hope you'll enjoy the photos and timelapse I took! 👋

Been a good week plenty of sun happy days.new greenhouse built.bring on the summer

WEEK 6 Things are going wonderfully with my plant. Thanks to LST, I’ve managed to create a really nice, even canopy, and the plant is already blooming actively. I expect a solid stretch in the coming weeks, and I’m curious to see how it turns out. If things keep progressing like this, it’s shaping up to be a truly great grow. Aside from that, I watered once and fed once this week. The plant seems to have responded very well to the switch to Plagron nutrients. I’ve also set my light to 500 PPFD this week.

This was a strain I’ve had my eye on for a very long time and happy to say I have now fully experienced her from seed until harvest ! The ladies were harvested on Day 58 of flower and were hung to dry. Very easy to grow, not sensitive to anything and likes it a bit dryer in her pot. Beautiful plant to look at while in bloom - completely covered in trichomes and lots of beautiful calyxes While in flower she smells of fresh fruit and and very pungent. After dry she smells of cheese, earthy and peppery. I would love to send her off for some lab results as the effects tell me she is high in thc! Will definitely grow her again :)00

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7 - 13 sep Inicia 3ro semana de floracion se realiza defoliación.

Flowering day 3 since time change to 12/12 h. Hey guys :-) It was about time that space in the flowering tent became available because the lady is already big in the vegi 😂👍. Now the time has come and I am really looking forward to the flowering. I put 20g of GHSC Bio Bloom on top of the substrate. was poured this week 2 times with 0.8 l. Have fun and stay healthy 🙏🏻💚 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 ‘Powered by GreenHouse Feeding’ Copy the link for 10% off all Nutrients 👇🏼 http://shop.greenhousefeeding.com/ affiliate/madelngermany_passiongrower/ 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : https://www.amsterdamgenetics.com/product/kosher-tangie-kush/ Water 💧 💧💧 Osmosis water with EC 0.0 Add Cal / Mag to 0.4 Ec and PH - to 6 - 6.4 MadeInGermany

I have checked the plants and one plant is now showing about 10% amber trichomes on old growth. New growth clear. Starting to see some nice colour changes across the canopy. Have fed them a weaker feed to start the week but included a microbe tea.

📆 Semana 6 Blue Zushi está mostrando su lado más exótico esta semana. Los cogollos han comenzado a compactarse con fuerza y se nota una producción de resina que va en aumento. Visualmente, empieza a asomar ese tono azulado que tanto llama la atención en esta genética, sobre todo en las puntas más expuestas a la luz. A nivel nutricional, sigo con la base de XpertNutrients y ya se nota el empuje adicional de Sticky Fingers, que está potenciando claramente la formación de tricomas y dando un pequeño salto en la intensidad del aroma, que empieza a volverse más dulce y fresco. Los Adlite están funcionando a la perfección: gracias a su uniformidad, incluso las ramas laterales están sacando cogollos densos y con buena pinta. Temperaturas estables entre 22 y 25 °C y humedad en torno al 55%, controlada con buena ventilación para evitar condensaciones y problemas en la fase más delicada. En cuanto a olores, empieza a destacar ese perfil afrutado y ácido con fondo kush, muy característico de la Blue Zushi. Los tricomas están en su mayoría lechosos, por lo que aún queda recorrido antes de pensar en cosecha. Una planta vistosa y con mucha clase. ¡Seguimos creciendo fuerte! 💪

Hey all! She has finally started to flower! To see the transition, its nothing but magical! I fed her Green Rush Bloom nutrients (1tsp/1L) with 1 L of water at pH 6. She has began to really stretch now. The light is lowered down at 16 inches. Will feed her Cal-Mag, and seaweed extract this week. Maintaining the RH between 55 to 65. Removing the fan leaves that blocks the lower buds.

+ Day 23: + watering : 2.5L bottle water EC 0.355 , PH 7.5 . Final EC 1.143 PH 5.98 New Light Evo 3-100 (200W) (80cm) in a 140x70cm tent . (Old : 3-60 (200W) (40cm)The two ladies are getting more space to grow! +

👉 ( Floração ) 👈 📅 Total de Dias 64 (F 34) - 05/10/2021 / 💦 Rega com nutrientes. 📅 Total de Dias 65 (F 35) - 06/10/2021 / Crescimento normal 😀 📅 Total de Dias 66 (F 36) - 07/10/2021 / 💦 Rega apenas com agua 📅 Total de Dias 67 (F 37) - 08/10/2021 📅 Total de Dias 68 (F 38) - 09/10/2021 / 💦 Rega apenas com agua 📅 Total de Dias 69 (F 39) - 10/10/2021 📅 Total de Dias 70 (F 40) - 11/10/2021 / 💦 Rega apenas com agua 👉 MARSHYDRO 👉 CODIGO PORMOCIONAL : Grow3rPT 👉 Em marshydro.eu 3% de desconto em qualquer produto

Harvest Report – Blue Sunset Sherbert The weed turned out absolutely phenomenal! The colors were stunning – incredibly dark, almost black, with deep purple hues that really popped. It was harvested at Day 80, with about 20% amber trichomes – just the right timing for a balanced effect. Check out the pictures – they speak for themselves! Stay tuned for the smoke report! --- Erntebericht – Blue Sunset Sherbert Das Weed ist einfach phänomenal geworden! Die Farben waren atemberaubend – richtig dunkel, fast schwarz, mit tiefvioletten Tönen, die richtig hervorgekommen sind. Geerntet wurde bei Tag 80, mit etwa 20% bernsteinfarbenen Trichomen – genau der richtige Zeitpunkt für einen ausgewogenen Effekt. Schaut euch die Fotos an – sie sprechen für sich! Bleibt dran für den Smoke Report!

Week 6 For Space Panda by Atlasseeds, End of week 6 for this cultivar, after the transplant the new growth fixed right up👌 This Strain has got some crazy root vigour was only 3 days since being transplanted.

increible crecimiento de las nenas. 5 semana ya proxima a flora como le encanta el bokashi a estas cepas de royalqueenseeds. se ven sanas y comen bien muy pequeñas, muy compactas me sirguen ayudando a darles mas vegeta.

Yo, what’s up, friends! Harvest day is getting closer 😍 Cannalope Haze is pretty much ready, but I’m holding off until Sleepy Joe catches up — I think just a few more days. The trichomes are mostly milky, with a few amber ones showing, which is exactly how I like it for a more uplifting effect to keep me productive throughout the day. For drying, I’ll hang the plants in my tent and aim for 20°C and 60% relative humidity. Let’s see how it goes—I’m excited to see the final result. Stay tuned for updates!