🌱: started this week with intensive LST and little Defoliation, Day 46 Lollipopping 💧 : 8l, 10l SIP 💡 : Dli: 45 mol/m²/d, 🤔: The plant has a rectangular area in the middle where it is not growing as quickly. The difference is almost 10cm. I suspect the lamp. Could it be that the centrally installed UV LEDs are triggering it? I noticed this in previous grows. There is actually less light outside, which can lead to slower growth or even stretching, right? I taped up the LEDs.

Venga familia que acabamos la sexta semana de estás Gelato Olandese de Dutchfem. Entramos en la recta final esta semana aplicamos el PK de Agrobeta, ExplotaCogollos. Iremos viendo el proceso de engorde de las flores. Se ve que tienen un buen color están bien sanas, se ve que progresan adecuadamente. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Las maximas de temperatura no superan los 26 grados y las mínimas no bajan 20, así que no me puedo quejar. Los niveles de humedad también son los correctos van entre 50%/65% de humedad relativa. Por supuesto el Ph lo estamos dejando alrededor de 6. Hasta aquí es todo, buenos humos 💨💨💨.

Day 17: They are growing well and healthy Callmag foliar everyday when the lights are off Day 19: Watered each plant with 0.4L with nuts 827 ppm, 1759 us/cm, 1.7 EC Day 22: Watered each plant with 0.5L with nuts 1010 ppm, 2148 us/cm, 2.1 EC Did some defoliation and lst aswell

Very excited

Las nenas tomaron bien el cambio fotoperiodo se nota como ya se van preparando para lo que se viene que serán otros 2 largos meses en su fase de floración. Se comenzó a aplicar Top Bloom de Top Crop intercalando los riegos entre agua, fertilizantes y micorrizas. Seguimos ...

This girl keeps growing. Started shortening her light cycle as she is getting really big. I have bent the main tops so often so that the rest can catch up. She looks great thought... Love this plant.

Saturday 16-11-24 , Flowering day # 53 week 8. Harvest day! Cut branches off plants & hung the 2 front plants off the light. Put scrog net back in with a large flyscreen laying on top, & spread branches from the back plant over it. This allows complete air flow around the buds. Running a single oscillating fan beneath pointing to the bottom. Temperature is around 24°C & RH is 55%

👉 Comienzo la floración: .- Paso a maceta final (14 Lts) .- Aplico Delta9 (primera aplicación en riego). En el siguiente riego añadiré Bactobloom de Agrobacterias. .- Hago una defoliación suave .- Enciendo los Quantum Boards de apoyo para floración, con el espectro Rojo Lejano e Infrarojo, para acelerar el proceso. La intendidad total es de 475w, iré aumentando hasta los 600~700w progresivamente. ⚠️ Observando el tamaño que ha alcanzado plantas en estas 5 semanas, pienso que debería haber iniciado antes la floración. Me temo que van a crecer demasiado para mi armario 😳

Also wir sind hier in ein etwas größeres Zelt umgezogen, letzte Woche. Die Pflanzen müffeln schon und entwickeln langsam ihre Blüten. Die gesamte Brut ist gesund und munter. Die Hollywood zeichnet sich zum jetzigen Zeitpunkt mit größeren Buds aus. Hab unten ein wenig MicroBuds gezupft und meine Finger rochen nach Kirsch/Himbeer Zitron Soda und rosa Hubba Bubba.

No update last week sorry got busy. In week 10 of veg they were cruising along and got bushy as can be from the heavy Defoliation. I wanted to give them a chance to really recover unlike usual before doing any more cutting or defoliating. Now we are in week 1 of veg and the single GMO Zkittlez needed nutrients so I gave it 21 ml/g of the A and B, 9 ml/g of Voodoo and Bud Candy and 10 ml/g of Cal-mag plus since I know it's a heavy feeder from growing GMO sherbet in the past. The rest will be getting the listed nutrient mix this week on their next watering. I defoliated them heavy this week again to allow any branches to stretch to the net that want too. I also took off any branches that were dying from being severely shaded, that just would be in the way later on, or were not worth keeping. I added in some green lacewings (eggs as we speak) to keep any pests at bay and prevent any future issues as part of my new organic IPM regimen. Everything looks amazing and I can't wait to see the end result!!

Hello everyone 😎 Week 3 of flower for the Banana Purple Punch auto from Fast Buds 💥 She grew fast with a beautiful green color,for the nutrient 4ml/L terra bloom & 1ml/L power buds from Plagron Mars Hydro SP-6500 70% Have a nice day 😋

The smallest plant i ever grow

Shitty diary update will be shitty🤣🤣🤣🤣🤣🤣time line is off but u know shit happens I can tell u this I had 3 different phenos one didnt make the cut #2 was kept because of her indica style growth but she is the sweetest smelling one out them all #1 was just a beast topped and still was over 4 ft she is coming down soon sum of the hardest golf balls from FB ✂️✂️✂️✂️✂️✍️✍️✍️✍️✍️✍️✍️✍️✍️✍️✍️✍️

Final week, had issues with PH. Lacked Nitrogen in the beginning of flower due to incorrect PH levels.. Oh well, still finishing this weekend.. 🌱🤔

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.