The first week of flowering is here, and things are finally looking much better! Both plants have recovered well and are now growing much healthier.🌱 #1 has already started stretching significantly, really picking up speed. It’s exciting to see how quickly it’s developing! Meanwhile. #2 spent about half the week still in vegetation, but towards the end, I finally spotted the first signs of flowering. Now that both are officially in bloom, I’m looking forward to seeing how they continue to develop over the next weeks. On to the next phase! 😊

Todo se ve muy bien por aquí hasta ahora, ya empezamos engorde. Esperemos cómo van respondiendo la siguiente semana que aumente las dosis. Muy feliz con los resultados obtenidos hasta ahora. Si que vale la pena dedicarles tiempo y ser paciente con el proceso. Seguramente la próxima semana iniciaré otros diarios con una cacería con unas regulares, una automática y otra feminizada junto a un esquejé de esta white widow que pude hacer.

Topping was great ! Starting now with LST using a wire but gently don’t want to harm the plant they opened up pretty good 🙏🏽 I am increasing water by looks.

Today: 14.18 hours of light 100.4 °F 58%Rh 1 million of mosquitos I've spent this hot week training these two plants trying to keep them the lower as possible since the two main buds have reached the roof (7.87 ft tall). Cookies Gelato is about 9.50 ft tall. I've applied white plastic net all around the pergola to give them some shade in order to slow down the metabolism. These plants are giant and there is no more space to move around. Asparagus stalks are well formed so this is, hopefully, the last preflowering week. Cookies is hungry and some bottom fan leaves start to show chlorosis but the top/mid canopy is plenty of natural nitrogen. During these last weeks I've watered them with 4-5 gallons each, leaving the soil to dry for 2/3 days, they seem to love this scheme. Also, the fact of not having pluck away even a leaf made these plants big and extremely vigorous. Tomorrow I will create a roof with clear plastic rolls as the nighttime humidity begins to increase and some summer thunderstorms are on the horizon.

Hello everyone, The girls are finally outside in 20 gallon fabric pots. This season I have a greenhouse for them and I hope it goes good. Still have to build the first cages around them and start stretching them. 🎊🎉 finally, summer is here 🎉🎊 See you guys next week 🤞🤞🤘🤘👊👊👊

Weather has been rainy and windy so she has been living under a shelter at nights and most days. The climate is getting moisty here so i decides to remove some fanleafs again to keep a good airflow. Buds are getting bigger and they make me wanna sing ' I like big buds and i can not lie..'

Veg36, están creciendo bastante bien, a esperar unos días para un próximo riego. Veg38, ya hay que revisar amarras y ver si hay hojas abanico tapando algún brazo, siguiente riego era en 1 o 2 días más. Veg40, se regó con EC 2.5 y pH 6.0, se cortaron un par de hojas, hay que seguir haciendo LST para lograr una altura uniforme. Veg41, algunas hojas están mostrando quemadura en la puntas por exceso de nutrientes, los siguientes riegos tendrán que ser más suaves.

WEEK 11) DAY1 Changed light schedule from 20/4 to 18/6 which will stay that way until harvest. Now I also start dropping the temperature 1 degree every 3-4 days. You don’t have to do this! I do this so the plants gradually acclimate to the lower temperatures like fall and then the beautiful different colors that come out from every individual Girl are different. (DAY2) light defoliation for an omelette Fill both water 6 gallons ph5.9, put an extra fan under WMZ#1 due to her size I might put a standup fan behind her. (DAY4) tuck leaves down all around the cola’s well my ac infinity 8” fan just broke only 4.5 months old ridiculous. (DAY6) water 6 gallons

Day 42 they filled the tent at 7 weeks.. wow.

Some mild defoliation this week. Budding up nicely and starting to frost. This girl was having a foliar feed of algae last week but this week she's getting light foliar sprays of the Lacalva stress treatment from madame grow on top of her other feed. I'll do this every 2/3 mornings and I'll only lightly mist the top. I'll concentrate most of my foliar feed on the lower growth and undersides, away from the buds. This is the main reason I kept so many lower fan leaves this time.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Semana 6 (7-13 de mayo)(días 36 - 42) Contentos con como va la cosa, retiramos alguna hoja tocada y poco mas. 😊😊 11/5/2020 El ultimo riego fue bastante heavy y podemos ver como las puntas muestran signos de sobrefertilicacion ,por tanto este riego va a ser más suave. Teniendo en cuenta que sufrimos el problema de quedarnos sin fertilizantes y que nuestro proovedor debido a la situación actual del covid tiene el asunto complicado tocaremos con lo que tenemos. Hoy toca riego en el que usamos 40l ñara 22 plantas. Los riegos se realizan en garrafas de 10l por lo que los valores varían en función de las plantas. (Valores • l) • 1gr de biogreen flower boost + 1ml calcio max + 1ml Mg PH: 5 94 EC: 1.21 T h2O: 18,5°C • 1gr de biogreen flower boost + 1ml calcio max + 1ml Mg + 0.2ml Final solution PH: 5 84 EC: 1.00 T h2O: 18°C • 1gr de biogreen flower boost + 1ml calcio max + 1ml Mg + 0.2 Final Solution PH: 5 81 EC: 1.18 T h2O: 17,3°C • 1gr de biogreen flower boost + 1ml calcio max + 1ml Mg PH: 6 EC: 1.44 T h2O: 17,2°C Para algunas variedades como la Bubba y la Barney Kid será su último riego con fertilizantes, a partir de ahora haremos riegos com flush hasta el momento de corte para que no queden restos en nuestras flores en el momento final. Al resto sin embargo les quedará alrededor de 3/4 semanas por lo menos. Esto sigue avanzando y se siguen formando nuevas flores pero esperemos que esto no tarde mucho en engordar, porque a pesar de que se que son de floración larga me da la sensación de que podrían estar mejor, no se si debido al cambio de nutrientes y de que no estoy cubriendo todas las necesidades que tienen. A pesar de todos tenemos unas Orange sherbert que pintan increíbles, una berry mint potente, dos chocolate mint OG que dan ganas de comérsela... Por comentar que ayer dejo de funcionarios uno de los focos 😔 tras echarle un ojo y hacer unas pruebas comprobamos que el fallo era del cable y ya esta solucionando 😁😁 Estoy disfrutando mucho con este cultivo y aprendiendo de los fallos, sólo viendo como van y con las cosas que he aprendido no veo el momento de comenzar con el siguiente cultivo, pero paso a paso 🤗 Por cierto estos últimos días la temperatura a bajado bastante por el mal tiempo lo que nos ha venido genial para estabilizar la temperatura en unos 25/26°C en ON y 22/23°C en OFF. Estos días atrás rondaba más los 28/29°C llegando en algún momento a 30. Un poco de frescura para el huerto. Y por último, pero no menos importante. Mi pareja, a la que tanto quiero, me va a dejar su móvil para sacar fotos. La diferencia es abismal por lo que intentaremos mejorar la cantidad de nuestro contenido para el bien de todos 😁😁

Peeking through the soil, my rainbow melon seedling is finally here! Its tiny leaves are a vibrant shade of green, and I can just imagine the colorful melons it'll produce. I'm excited to watch it grow and can't wait to share the sweet and juicy fruit with family and friends.

Week 2 vegetation for the ztrawberry from fastbuds 420. All good at the moment, we are testing the xpert line with these girls. 1ml of master grow A+B 1ml of master root We removed the 2 lowest sets of leaves and branches. We do this because we have the girls in 7 liters and quite some plants together, so i prefer to concentrate on the main bud and only some side buds

Que pasa familia, vamos con la Segunda semana de crecimiento de estas Royal Gorilla de RoyalQueenSeeds, que ganas tenia de plantar esta variedad, no me cansaré de repetirlo. Han progresado muy bien de las 7 que quedaron las 7 están bien verdes y van creciendo a buen ritmo. Aplicamos en nutrientes la base de Agrobeta de crecimiento, y también empezamos aplicar bien con tucán y gold Joker. Espero esta semana poder empezar con los trasplantes que también tengo ganas de pasarlas a maceta definitiva que ya lo van pidiendo. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Hasta aquí es todo,nada más: Agradecer a James de Royal, Jose de Agrobeta, y a la empresa de Mars hydro, por los productos, que sin ellos estos proyectos no son posibles. Espero que lo disfrutéis, buenos humos 💨💨

This is war now boy @chedderbob112 wakey wakey @cheddersteve123 s2340420 remember boy am watching

HEy guys so another week has passed and it is getting close to end for my lemon ak girls . Begun flush this week passed 10 gallon through eachnplant and strip back all main fan leafs to e,pose buds . The remo is a monster ready to flip for sure and i will repot blueberry llater to 3 gallon til i swap again to 9 gallon after auto done . All look good sorry again light i haven't got a good led yet and i have be cautious where i stay .- 😉