🗓️ Week 12 – Flower Week 8 ✅ (Late Flower) 🔹 General The plants have now entered deep late flower. Buds are gaining more density and resin, and the aroma is intensifying day by day. For Cherry MAC Muffin, fruity-sweet notes are becoming dominant 🍒✨ 🔹 Feeding After a longer phase of reduced EC, feeding has been increased again. Input: 1.65–2.0 EC / pH 6.3 Runoff has built up to ~3.0 EC, but remains stable – no signs of overfeeding or stress. No flushing yet – the final flush with Flawless Finish is planned in about a week 💧 🔹 Observations – Cherry MAC Muffin 🍒 Development: Densest buds in the tent, very sticky and frosty 📏 Runoff EC: 3.0 ✅ Status: No stress symptoms despite the high EC – watering frequency slightly reduced to maintain balance

Everything looking fine so far. I gave them a little amount liquid grow enzymes with distilled water on 19.04. what seemed to boost the growth. If You have any tipps or recommendations feel free to let me know.

UPDATE GANG!!!! Sooo im keeping up with it, as you can see we are starting to see the flowering in the video LETS KEEP IT GOING!!!!!!!!!!!!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hello Diary. I am very happy that we are starting again. I’m going back to Automatics again. Many thanks to Royal Queen Seeds for sending me two of their new strains, Green Gelato Automatic and HulkBerry Automatic. So this is the first of two new diaries. WHY GREEN GELATO AUTOMATIC? Green Gelato Automatic is the autoflowering version of a legendary cannabis variety. The original strain is the lovechild of Thin Mint Cookies and Sunset Sherbet. It's one of the most aromatic strains available and produces monstrous levels of THC. Green Gelato Automatic contains both sativa and indica genetics. Her effects can be felt in the body and mind simultaneously. In fact, with a THC content of 24%, she's one of the most potent autoflowering strains you can find. She sends the mind into overdrive and makes a perfect smoke when writing, painting, or doing anything creative. The strain also boasts medicinal properties, taking the edge off pain, elevating mood, and raising appetite. LET'S START FIRST WITH THE FARM SET-UP: Box - Secret Jardin DS120W 120x60x178 Lights - MIGRO 200+ Ventilation - Blauberg Turbo - E 100 Fan - Oscillating Koala Fan Humidifier - Beurer LB 45 Soil - BIOBIZZ Light - Mix Pot - 11L Fabric Pot from RQS Seed - Royal Queen Seed Nutrition - BioBizz and RQS Organic nutrition I apologize in advance for the detailed documentation, or the large number of photos, but I enjoy it, and I think it will help others around tracking their plants. LET THE DIARY START OFFICIALLY: 10/06/2020. planting. I have prepared everything I need for planting. The 11lit fabric pot I got from RQS, also Organic Boost Nutrition and Mycorrhizza from RQS, Soil is Light-Mix from BioBizz and of course the seed of the new strain Green Gelato Automatic. After adding 50g of pellets from Organic Nutrition and 5g of Mycorrhizze, I mixed everything well in the pot. After that I watered well and soaked the soil, I dug a hole with a wooden stick about 2-3 cm, I first put 1g of mycorrhize and then put the seeds inside. I lightly covered the hole with earth and put the pot in his new home. The temperature was about 25 degrees and the humidity about 65%. 14/06/2020. She sprouted. After 4 days, Green Gelato came out. In the meantime, I sprayed the surface of the earth a little twice. The temperature is still around 26 degrees, thanks to the summer and the high temperature that enters the apartment anyway. I regulate the humidity with a humidifier, I maintain it around 60% -65%. 15/06/2020. Her first photo shoot, I also watered her with about 2 liters of water, which I only regulated p.H. at 6.3 with Plagron, Lemon Kick. 19/06/2020. Day 1. Green Gelato is officially one day old. On that occasion I took another photo shoot to celebrate this big day :). 24/06/2020. First Feeding First feeding. She got a whole meal from BioBizz, Root-Juice, Bio-Heaven and Acti-Vera, p.H of water I regulated with Lemon Kick from Plagron. 25/06/2020. Day 7. End of the first week. For now, everything is great. It is developing nicely, its conditions are almost perfect. Green Gelato will also have two roommates in the box, HulkBerry Automatic. Green Gelato - Day 7. - 10 cm I think that’s all for this week, I’m happy that it’s starting all over again, following its development and looking forward to what’s to come. See you next week.

Only one plant to harvest🤞

Day 42 - Ladies are growing beautifully & starting to smell extremely strong! The Gorilla Girl is short but very bushy, I think she will have some massive buds but we will see! Thanks for following & happy growing friends!🙏🏼✌️🏼🌱

The plant grows. I have now added an oxygen pump to the water tank. Let's see what effect that has. You can see the progress every day. I've also bought some phosphoric acid to keep the PH down (hopefully). My water tank is very large, about 75 litres. I also have a few holes in the lid, so a lot of rainwater gets into the tank through this and also through the openings in the pots. Never had to top up the water yet lol. At the end of the fourth week I'm happy with the growth, especially as it's my first plant. -------------------------- Die Pflanze wächst. Ich habe mittlerweile einen Sauerstoffpumpe in den Wassertank gegeben. Mal sehen wie sich das auswirkt. Man sieht den Fortschritt jeden Tag. Ich habe außerdem Phosphorsäure gekauft, um den PH-Wert endllich stabil unten zu halten (hoffentlich). Mein Wassertank ist sehr groß, ca. 75 Liter. Außerdem habe ich ein paar Löcher im Deckel, so dass dadurch und auch durch die Öffnungen in den Töpfen viel Regenwasser in den Tank gelangt. Musste noch nie Wasser nachfüllen lol. Am Ende von der vierten Woche bin ich zufrieden mit dem Wachstum, vor allem da es meine erste Pflanze ist.

She has really shot up in the last week. She has grown a nice even canopy all by herself. I have given her a second and final feed of dry amendments.

Hi growers! My kushes are on FD 19.Not much streching thise time.Bubba Kush is al litle yelow,but showing nice grow.Started to add green sensation. Stay green🌱

i looked awesome all growcycle...i streched alot & i noticed it drank alot more then the other plants while growing...had a good yield, i´m very happy overall

Only the tropicanna left. In a week/ couple of day I will harvest the lady. I decreased the addition of nutrient and the last 2 days I will change all the water in the pot with pure water (ec 0.4-0.5. The smell is strong and juicy. Tropicanna is at least x2 bigger than the strawberry. If all 2 plants were big as the tropicanna I have had issue with the space in the box. Fortunately is all fine

Starting 4 week! I have moved 2 Monster and the strawberry in a 11L pot and moved the dosidos and Gorilla in 6,5L pot. I did a light Lst son Monster Zkittlez, they are growing faster then others. Overall at moment it’s a good result but temperature are still high and for this reason I think I will give them probably around 10 daysmore in the growing stage! Happy growing !!

Very very sticky i had to change gloves every 2-3 buds!🤓 Delicious smell🤤

🍍F A N T A I should give them around 2 more weeks to fully mature but got no more time start my holidays