After you found some good sector and clone it you let the mycelium grow and fill almost all the petri dish like I did last week.
So now you're ready to move forward.
Making grain spawn!🍄🍄
For grain you can use Organic Rye Berries, but you can also get away with wheat berries, or brown rice or even corn kernels(which I used myself) !
And you'll need some mason jars or honestly I used some jars that I had home just clean them very well and sterilise before using
Making a grain spawn can be broken down into 6 easy steps.
🍄Hydrate Grain By Soaking
🍄Make Specialized Lids
🍄Simmer, Drain and Dry The Grain
🍄Sterilize
🍄Put your agar in the jar
🍄Colonize
🍄Soak the grain by placing it in a bucket, covering in water, and leaving it for 12 -24 hours. Easy.
🍄Drill 1 hole on top of the lid. Right in the center and cover with a small dab of high temperature silicone sealant, and leave it overnight to dry. And your jars are done. There other diff types for different methods like liquid culture etc. But I'm just going roughly through what Ive done.
🍄The next step is to simmer the grains on the stove. This will thoroughly hydrate the grains, and will soften them to make them easier for the mushroom mycelium to consume.
Cook soaked grain on the stove for about 15 minutes. You don’t want to cook it too long, because eventually all the grain will crack and you’ll get some mushy grain spawn. We don't want that. 🧐After the grains have simmered on a low boil, you’ll need to drain them off. What I did is I drop them in the sink with the drain cover on. The grains will drain over an hour or so, after which you can go ahead and fill your jars.
🍄Once your jars are filled, they need to be sterilised.This kills off all the other nasty contaminants in the grain cover the lids in tinfoil (to prevent water dripping into the lid) and then just pressure sterilise them for about 90 minutes at 15 psi.
🍄After that let them cool down. Get your SAB, wipe down the walls with some alcohol wipes and place the jars inside. And wait untill they will be warm to the touch... not hot and painful 😤patience is key. Bring in your plates that had been stored by you in a dark place. And what u have to do is sterilise your scalpel and just accurately and efficiently decide your mycelium into about 10 pieces and quickly place them in your jar. And complete for every jar u make. You have to be very quick. The more you keep you're jar and your agar plates lids open the more cans they will get contaminated. There certain technics you can see online..
🍄Ones you're done close all your jars lids tight and Jake your jar so the mycelium pieces will mix around the jar and place them in the dark place to colonize. And just keep waiting... 😅 Untill the mycelium will conquer your grain. Keep your eyes on the jars, any weird smells or colour during the waiting time.. because that would mean you got a contamination going on. Oh yeah and when about 55% of your jar is filled with mycilium shake the sh*t out of it so it will break the mycilium inside and make spread more faster.