Just on plain water now. The plants have definitely put on a bit more weight in the last week but they're very nearly finished and just waiting to ripen. Trichomes are all cloudy and I can only spot one or two amber trichs so will wait another week to see. Plant 2 looks like it could possibly go a little longer so we'll see. Tiny bit of purple coming through in the buds of Plant 1. Both plants have a real wobble on now with the weight as seen in the video. Have really enjoyed growing this strain. Buds look dense and sparkly. It stinks to high heaven and should have good bag appeal. Just keen to get them out the tent to give the light over to the other plants.

I love these last stages! The girls are still stretching but I decided to give them a defoliation a week early. If you look back on last week you can see how bushy they have gotten so there was a definite need to get some light to the lower bud sites. I can definitely start to smell the familiar gassy aroma coming through now so may be time to get the fan and carbon filter cranked up. I will be top amending the soil again at some point this coming week but as for now I have just been feeding with water and liquid seaweed. I've had a few compliments from friends on the good health of the plants this time round compared to previous grows when the Sour Diesels had nutrient issues, not helped by the Coco medium I was using. I'll be sticking to this choice of soil and organic nutrients with the results I'm getting so far! I mean, look at those pistils!! 😁 I love that I've had 93 views on this diary so far! Thanks to everyone that's checked it out but I'd love some feedback? If you like what you see please drop me a like and if not, let me know what I can improve or do differently? I've got another video for you guys from today so I hope you enjoy it :). Until next week! ✌️

Week 2 7/26- All the girl's are doing great. I had to use some horticulture wire to prop up a couple of the girls that got a little 'top heavy'. Giving 12oz water ph'd to 6.3-6.5 as needed 2 hours after 'lights on' around each plant, and every other day in the saucers (1/4g/ea) to allow the medium to wick it up and stay moist. Temp's running 72-81deg with an average RH of 55-60%. I'd like to have the RH a bit higher but the girl's seem to be doing fine at the present. 😎Ordered another HLG 650R with the supplemental UV Bar to replace the two SunSystems 315 CMH's....should be a game changer! 💪 Also have an AC Infinity 6" exhaust system w/controller on the way to replace the Hurricane 400cfm fan I'm currently running along with another TerraBloom 6'x24" carbon filter as my current filter is losing efficiency. 7/27- Watered today ph'd to 6.4 with 2 tbsp/gallon unsulfured molasses added. I split 2 1/2g between the eight girls by pouring 12oz around each plant and then dividing the rest in their saucers to be wicked up. Temp's, RH and CO2 all on point! 7/28- Light watering today with straight non-chlorinated water ph'd to 6.5. Growth is looking great across the board. Even the late starters, Seedsman Zkittles #1 & #2, are coming along nicely! All the ladies are averaging in height between 2 1/2' - 3 1/2" and should be getting ready to explode as their root systems become more established and are able to start taking in more nutrients. Fans are running 24/7 to strengthen the stalks....they're gonna need it to support the buds these ladies will be sporting in a couple months! 7-29- Watered all through the bottom today with straight .5g non-chlorinated water ph'd to 6.3. Temp's, RH, CO2 and VPD all on point. Unhooked carbon filter from hose as it's losing efficiency restricting exhaust air flow. Girl's all growing STRONG especially the #1 Seedsman Gelato OG in Fox Farm/Nature's Living Soil mix and the FastBuds Purple Lemonade #2 also in the Fox Farms/Nature's Living Soil mix with the Seedsman Gelato OG #2 in SOHUM soil is getting close. I appears the two girl's in SOHUM soil are not quite as tall as the others but are leafing out well and are stocky with close node spacing.....hmmm? 7/30- Lift checked all the pots and gave 2 1/2g straight non-chlorinated water ph'd to 6.4 with 2 tbsp of unsulfured molasses added. I split the 2 1/2g evenly between all eight giving 20oz through the top and the remainder in the pans to be wicked up. I turned up the HLG 650R to 400w at the wall and kept the height the same. My AC Infinity Cloudline T6 exhaust and new Terrabloom 6x24" carbon filter came today, as did the UV bar from HLG for the HLG 650R that'll be here on the 3rd.....CAN'T WAIT!!! All the girl's are really doing well and starting to begin 'gettin it on'! All are healthy and running between 3-4" tall. Side note: While installing the new exhaust fan setup on of my pooches sneaked in the room and munched off a half of a shade leaf on the #1 Purple Lemonade....she's definitely got good taste!😆 In the process of installing the AC Infinity fan I relocated it and the filter to the left side of the tent and re-routed the cold air intake hoses so that both enter the tent at the bottom and are positioned at either side of the tent. I setup the AC Infinity which is a breeze, set all my high/low limits and alarms and let here run! Temperatures now are running 72-81 deg. and RH is remaining stable @ 55%, CO2@800ppm. All in all a good day!😎👍 7/31- Water looks good on all the girls. I'll just top water lightly today with straight de-chlorinated water ph'd to 6.5. The AC Infinity is really a 'game changer' as temp's are now staying in a stable range without having to adjust the settings on my Ideal Air 14k BTU AC unit...niceee! All my ladies are finishing up their second week like champs and I'm looking forward to the next few weeks as when their root systems reach the 'super-soil' they are going to hopefully have some explosive growth. I'll also be evaluating all of them in the coming week to determine candidates for topping and LST to begin. 8/1- The second HLG 650R came today! I tidied up the tent before installing the 650R: moved EXHALE CO2 bags-one on each side, vacuumed, took care of any loose hanger cords and re-positioned the three thermometers/hygrometers. I installed the second HLG 650R w/UV bar in the tent and set both lights to 400w @ the wall and have both boards centered, level and hung @ 36" from the soil. These lights are AWESOME! I checked temp's on the boards & drivers after they were running for 4 hours: 98deg on the drivers and 94deg on the board...pretty freakin sweeet! Gave the girl's a day off from water as they all looked GTG and are all getting ready to really turn on! I plan on hitting them early tomorrow morning with a good watering. Temp's-RH-VPD all on point for this week. I've been continuing to run the humidifier set @ 60% RH and I'm going so far as to ph the water going into it to see if it makes a difference as my well water runs 7.8ph @225ppm dissolved solids and I hypothesized that as the plants were absorbing the moisture from the air it might be beneficial to have that moisture at the correct ph.....we'll see I guess!

Been a struggle since go with too much moisture. had to transplant to a hydro soil. Plus started using smart bags. Hopefully my issues are resolved and she grows to a pretty little plant. I imagine I have stunned it pretty badly. Still alive though. Thanks again for likes and follows.

This week we should really see these colas blow up and start stacking really strong. The mixer of 7 hours of sun and 17 hours under my LEDs has really helped my girls grow strong. Nothing compares to good old Mother Nature.

Great starter plant for a first timer was easy all round she stayed short but stocky rock hard dence nugs

Wow! What a difference a week makes! The purple leaves are really showing now in the Glueberrys & Colorado Cookies. 7 Glueberrys, 2 Colorado Cookies and 1 Blueberry will come down in a few days. Upd

*Week 5 Flower 09/24* Thus far both both plants are flowering and budding nicely, Trichomes are setting in accordingly, Increase in Phosphate and Potassium nute feeds. Both plants have appeared to stop growing in height settling in at 48Inches. Top COLAS are pretty dense and the aroma coming off both of them is bright and vibrant White Hairs are starting to turn bright orange. *Week 5 Flower Mid week update - 09/28* Both plants have reached max height (48 Inches) Bud sites are filling up and have become more dense with trichomes cover through the flowered bud. 60% orange hair coverage - Nearing harvest *Week 5 Flower End week recap 09/30* Both Apple Fritter and Critical+2.0 nearing harvest Cutting back on nute feeds starting tomorrow and will increase clean water feeds Flushing will begin in a week - both will be harvested in 2 weeks.

this week I defoliated so many leaves and continued LST. EC is around 1.0 this time. Everything is going well.

I like her indica structure and I start to train her , she has a little nitrogen deficiency but it’s ok now . She’s ready for next steps

Einfach schön zuszusehen, wie die Sex Bud an Masse zulegt. Die Farben überaschen jedes Mal wenn man das Zelt öffnet. Der Geruch wird langsam merklich stärker. Wir haben die ersten braunen Trichome entdeckt. D.h. wir gehen in den Endspurt. 👽

Doing amazing!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Esta semana creció muy bien, la maceta que había buscado de forma provisoria anduvo muy bien ya la próxima semana voy a buscar la maceta definitiva para floración y arrancaré con un poco más de luz para poder dar un poco más de flores con un poco más de resina muy buena la cepa fuel og de Ripper seed, la verdad que supera mis expectativas día a día muy buen crecimiento muy buen olor excelente resistencia al estrés y buena tolerancia los calores y a las cambio de temperatura.

Pretty much same as last week, I had another plant herm and kicked it out of the garden. I’m down to 16/50 seedlings that I had, but I was expecting that, which is why I did so many. Things are looking pretty good though! It does seem as if only some of the branches/colas on some of the plants were pollinated, which apparently is possible? I also started to flush the one girl that is furthest along, I’m thinking she will be ready for harvest within 1-2 weeks based on how fast she’s been progressing. Anyways looking forward to the coming weeks! Happy growing everyone 🌱

Pheno dx: 30g and only one photo Pheno sx: still drying

1 week more for this

They seem to have packed on some decent size this week which I’m glad to see but the trichome production leaves a little to be desired. Some of the lower buds look more frosty than the top buds which seems weird to me but I don’t really know that much lol. My ph runoff has been a little low which I think has to do with the molasses I gave them which wasn’t blackstrap which I think raises ph. Anyways, next watering will be with about 7.0 until my runoff corrects, it could explain the yellowing leaves as well. But the runoff is 6.1-6.3 which shouldn’t be that big an issue so it could be something else but I’m so close to the end messing around too much is probably not the best thing to do. Hopefully my pictures and videos are a little better this week at least! Enjoy and hit me up in the comments if you have any tips!

This is the last day of her life. Shes getting a bath of sun with 40c outside. After she will finish in the dark until i harvest her tomorrow Shes trying to get pollinated by throwing some new pistils here and there, but trichomes are cloudy mostly with amber tone. Not a lot of clear one.

N deficiency but it’s ok