2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Welcome to hyper glue @Anesia_Seeds grow feminized seeds Super Glue x Apple Fritter Yield: 600g/m2 • 700 g/plant Genetics: 60% Indica / 40% Sativa Flowering time: 8-10 weeks Outdoor harvest: end of september suitable for indoors and outdoors Height: 120-150 cm • 150-200 cm THC: more than 29% Aromas/ flavors: apple and red fruits 6/9/21 Made my plans today for next grow Wrote schedule down work very good last time to see if plants would go together will see ore the madness works 7/9/21 LET'S GET TO GROWING GROWMIES The germination can begin 🤘🏻😎🤘🏻 🤘🏻🤩🤘🏻 🤘🏻🤘🏻 9/9/21 Paper towel time 🤘🏻🤘🏻 10/9/21 I planted them today in a Easy start Let's hope for the best 🤞🏻 12/9/31 But one hyper glue in bigger pot other is still in easy start muchas gracias compañero por el gran paquete. Haré mi mejor esfuerzo visit www.marshydro.eu for your best gear and use the PROMO CODE: DEVILSBUD Fully marshydro supported Setup: Marshydro GT100X100X180 Marshydro Ts 1000 LED lamp soil :Plagron batmix Advanced Nutrients pH Perfect Bloom pH Perfect grow pH Perfect micro B-52 Voodoo Juice Rhino Skin Big bud Bud candy Overdrive flawless finish Sensizym

12/11/2021 - held in a glass of filtered water for 24 hours and then used the paper towel method for 48 hours. Seeds planted today in 6 litre pots to germinate before being moved to 15 litre pots on week 3 of veg. high humidity this week - 70% - and 100ml of filtered water every other day. 16/11/21 - all 9 have broken the soil and are stretching slightly to the light 20/11/21 root juice

Planta cresceu em repouso nas últimas duas semanas e voltarei a praticar o LST na próxima semana. Planta está crescendo em um grow com outras 4 plantas em estado vegetativo e uma em revegetativo. todas as outras 4 plantas da mesma semente não germinaram.

I'm very happy with this weeks progress. They transitioned very well to the garden, which I'm chocking up to the row cover. They didn't need any water due to the thunderstorm we had last night, but I did make a ring around the base of each plant and gave them about 1-2 TBSP of the Coast of Maine Stonington Blend fertilizer. They also got their tomato cages.

- harvest was today on day 59! (31/03/2019) - sooo bummed that she is so tiny because the frost and smell are just insane, its like fresh made orange juice - as usual harvest report will be up in 10 to 14 day when everything is dry so stay tuned

Always a huge yielded and a heavy hitter. Name is true to the effect. A little tough to get back ahead if you get deficient so remember she eats a lot. My favorite strain, have had the cut for over 2 years.

So this week i Can only enjoy how much she's growing. I had to do some more defoliation on day 23. I surely should defoliate way more but wanted to try like this. On day 25 , I just enjoy the view of that Wonderful plant😊

Day29F - still showing vast growth on buds, swelling and trichomes blossoming out. The colours starting to form nicely on the buds. Smells like a pungent caramel now. Day32F - This plant is so thick and strong, and can resist a dry and hot climate. For me this plant has been so easy, and she sucks up nutrients and loves it, 💪 Buds are getting dense and so sweet to smell. So fat and so many, I'm surprised on this auto 👏 well done again RQS. Day 68- Day 33F - the buds continue to swell and devolope. Trichomes flooding through now. Starting to see cloudy so will push for another week of nutes if she'll allow it after Friday, we'll see 😏✌️💚

Started my first feeding at half recommended dosage of fox farm feeding schedule

Truly, starting to show their beauty cannot wait until they start packing on the buds🔥💯

Blue Dream is now in it's forever home, but I will keep them in veg for a few days. Maybe a week

I Messed Up A DAY Here. So one of the days is dated wrong. I tried to correct it the best that I could. 7/24 I decided to label this week as flower. It's more like preflower though. GMOs are stretching like crazy. Due to the rain we weren't supposed to get some of my plants are a little over watered. That's why I love the fabric pots. With the winds we get the bags dry out fast. The GMO's would've needed water anyway if we didn't get this storm. Event Horizon seems to be the furthest in flower amd the most over watered. WE'LL get a LITTLE more rain today and tomorrow but then hopfully my bags will dry out. They are heavy as hell right now. Had we not got rain for what seems like forever. Several plants would've needed to be watered if we didn't get this rain. Still the plants are currently overwatered. All the pics and video so far are from this morning after the horrible pounding rain of yesterday. Like I said before, I need to; defoliate for better air flow, add secondary supports or a vertical trellis, considering using a preventative pest application (e.g. BT), LST and spread branches apart and start using nutes. 7/25 Wasn't supposed to rain today. Like .001. It poured this morning and is still raining VERY LIGHTLY at 2:30. We got an inch yesterday. The total for these 3 days was suppised to be under half an inch. Anyway I went over around two and everything looks great! Things are clearly overwatered and heavy but the plants look happy! Most have their leaves out and everything! I love growing in bags because they dry out so quickly! I don't mind watering more. I defoliated one event horizon and the few yellow leaves I coukd see on other plants after shaking them off. The seedling in the 50 is far behind the others in flower and just showed its sex. I'm fairly certain that one is red runtz. Whatever it is is going to flower much later than the rest. It's going to be good if I have couple early finishers. I did a video but I'll have to upload tomorrow. I have a lot of work to do And not just in the garden so I'm hoping I can balance things out and get this stuff done. Caught three TINY inch worms. I'm wondering if me manually hunting has cut the adult population down and I'm just getting fresh born ones? If that's the case I wouldn't need to spray. 7/26 Plants looked pretty great today. I think me manually removing pillars has made a big difference. It's windy out too so it won't take long for those bags to dry out. Which is good because what wad supposed to be a half inch of rain turned into 3 straight days of rain. I'll be back over to work on the garden today and I'll update then. 7/27 Plants are looking pretty good. Good weather ahead and the bags are starting to lose weight. Found a pillar wrapped in a dead interior leaf all wrapped up. Found a couple more early this morning amd killed them. I did some slight leafing on some plants. I don't like doing that outdoors but since these are monster cropped they have far to many leaves. Airflow is improved. I'm not seeing any nutrient deficiencies yet. Plants are a vibrant healthy dark green. Soon I'll add a base nute like big bloom or ancient amber (I think that's the one, it's applicable to big bloom). I cam see some plants trying to start flowering and I've got one (in the 50) that has just shown sex. Hopfully this will give me some time between harvests. I'll update as I go along. DAD CALLED IN THE AFTERNOON AND SAID HE THOUGHT A PLANT MIGHT BE STARTING TO DROOP. IT WAS MY GMO CANARY THAT GETS HIT ON ALMOST THREE FULL SIDES BY WIND (ONLY ONE PLANT). I CHECKED THE WEIGHT AND IT WAS LIGHT. IT WAS DROOPING TOO. I WATERED THAT ONE PLANT WITH A GALLON AND WATCHED IT PERK BACK UP. I CHECKED THE WEIGHT ON THE OTHER PLANTS AND LIKE I THOUGHT THEY WERE STILL HEAVY. I PUT MY FINGER IN THE SOIL AND IT CAME AWWY COVERED IN DIRT. MY SEEDLING IN THE 10 WAS KINDA LIGHT SO I GAVE THAT HALF A GALLON. I USED THE OTHER HALF AS "A CUP OF KINDNESS" ON THE PLANTS THAT SEEMED LIGHTEST TO CARRY THEM OVER. I PROBABLY SHOULDVE WAITED BUT I DOUBT IT MATTERS. ILL CHECK IN THE MORNING. I ASSUME ILL HAVE TO WATER THEN OR AT NIGHT. ILL GO BY THE WEIGHT. THE CANARY WILL NEED TO BE ON A SEPERATE SCHEDULE. THATS FOR SURE. Went back over at 6:30 and plants looked better than they have in a long time bags still have some weight. With the sunny weather we are going to have these plants will keep exploding in growth. Found one pillar but I wasn't there long. Seriously considering a spray of BT in the near future. EDIT: UNFORTUNATELY MY SPIDEY SENSE STARTING TINGLING AROUND NOON. MY ANXIETY WONT LET ME IGNORE IT (IM NOW EMBRACING IT) AND I HAD TO CUT OFF A CONVERSATION WITH MY WIFE TO GO TO THE GARDEN. I IMMEDIATELY SAW THAT ONE OF MY GMOS WAS STARTING TO DROOP. NOT LIKE BEFORE BUT IF I HAD BRUSHED IT OFF AND LEFT IT FOR FOUR MORE HOURS IN THE 90° SUN IT WOULD'VE BEEN. I GAVE THE GMO'S (BESIDES THE ONE I GOT YESTERDAY EACH A GALLON. I DIDNT WANT TO WATER DURING THE DAY BUT THIS WAS MY BEST OPTION. I GAVE THE TOASTED TOFFY A GALLON AND THE 2 EVENT HORIZONS EACH GOT HALF A GALLON, AS WELL AS THE SHERB PIE WHICH GOT HALF A GALLON. I DECIDED TO DO THIS BECAUSE SOME PLANTS USE MORE WATER THAN OTHERS. BEFORE ACTUALLY GOING OVER I CONSULTED MY PREVIOS DIARIES. I HAD THIS SANE PROBLEM LAST YEAR ON THE SAME WEEK OF THE MONTH! THATS WHAT MADE ME TRUST MY INTUITION FULLY. I LEARNED FROM THE MISTAKE OF LAST YEAR. THINGS ARE GOING GOOD. IM STARTING NUTES SOON AND I NEED TO SPEAY FOR BT BUT DONT KNOW IF IT WILL BE TONIGHT. 7/29 Apparently my intuition worked out well. Plants looked great this morning. I may feed today. I defoliated a bit and watered the 10g with a half gallon and gave another half gallon to the gmo on the end that dries out fastest and that I didn't water yesterday. I think I've got the watering down. Now I need to start nutes and spray bt. Need to wait for the rain first though. Suppised to get minor showers the next couple days. I'm gonna try to get the trellis up too. WENT BACK A LITTPE AFTER FOUR. HUMiDITY WAS 100% AND IT WAS RAINING VERY LIGHTLY. I MEAN AS LITTLE AS IT CAN POSSIBLY RAIN. ALMOST LIKE WHAT IT FEELS LIKE WITH THOSE MISTING FANS IF YOU STAND BACK A WAYS. I DIDN'T WATER. PLANTS WERE LOOKING GREAT! I DID SOME DEFOLIATION AND CAN TELL I NEED TO START NUTES. I CAN SEE SOME SLIGHT DEFICIENCIES IN BIG OLDER LEAVES. SOME SMALL INTERIOR LEAVES YELLOWED. NOT MANY. GRANTED THEY ARE STARTING TO FLOWER. I'LL PLAN ON FEEDING TOMORROW. THE NEXT FEW DAYS IS SUPPISED TO BE SHOWERS. I DOUBT IT WILL GIVE MY BIG GIRLS ALL THEY NEED BUT IT WILL BE A GOOD START. I DID A VIDEO BUT ITS RATHER LONG SO I CANT UPLOAD IT HERE. ITS COOL WATCHING HOW DIFFERENT THE DIFFERENT STRAINS GROW. STILL HAVE A BUNCH OF SHIT TO DO. IT WILL GET DONE THIS WEEK. 7/30 I held off watering today due to a supposed storm and rain we were supposed to be getting. I had early doctors appointments. I got back around 11 and the bags seemed light. After consulting last years diary I saw that I am underwatering. The root system weighs a lot! We haven't got the hundredths of an inch of rain but we got some sun. It's over cast now. I was there working for a few hours. I watered most a gallon. All the gmo's got a gallon. I gave the two event horizons about two thirds a gallon. About the same with the toasted toffy and the sherb pie. About 3/4 of a gallon as they seemed to have a little weight left. Looking at last years diary there were plants I was giving two gallons at a time in a 20 gal bag so I've been u Der watering a bit. I've caught it now and adjusted. I'm not losing leaves at the pace I have in the past. Even though some are in flower. I think I'm getting better. I fed for the first time today. Each plant got 1.75 pints. Except the 10gallon which got half the powerade bottle and the 5gal which got a qtr. By the time I left the plants were standing up nice and tall and looking amazing. Now I just need to apply bt and secondary supports. 7/31 Another eight day week. I'll have to do six days next week. Everything looked amazing this morning. I'm seeing less pest damage. Watering and feeding was the right move. Now I just have a little rearranging to do, add supports and spray if I decide to.

Watered with Real Growers Recharge and moved the main air circulation fan out to blow cooler air in. Tucked and tied down some larger leaves to allow for better light penetration to lower sites. I topped a few of the lower branches (couldn’t help myself) and have good regrowth so far!

Régénération de la culture du printemps que j’ai ressorti dehors au mois d’avril et que j’ai re nourri et elle est reparti on va essayer une 2 em récolte. C’est vraiment un essai pour le fun

I'm really contemplating dropping Bruce & Diesel. They're developing very badly. We'll see how they continue to develop, another week and it will be clearer.

With 33 grams on such a small plant I can't complain. I was amazed on the many hours (3) to clean the buds before curring. Several buds had rotten places because of the yellow leaves, they died and caused this problem Luckily I stopped in time and dived a little bit earlier in harvesting here highness.