Que pasa familia, vamos con la segunda semana de vida de estas Rainbow melon feminizadas de fastbuds. Vamos al lío , las 3 semillas que germinaron con éxito se colocaron en macetas de 0.6 litros y asomaron 3 , 100% ratio germinado. Necesitaré 2 ejemplares d esta variedad, en el trasplante quitaré la más pequeña. El ph se controla en 6.2 , la temperatura la tenemos entre 20/22 grados y la humedad ronda el 50%. El ciclo de crecimiento puse 18h de luz, el foco está al 75% de potencia. Hasta aquí todo, Buenos humos 💨💨💨

Eine neue Runde!

Let’s Go Day 81!!!! This week went real great 2 Ogreberrys are hanging to dry and also the Bruce Banner is too after there 48 hr of darkness! The 3 other Ogreberrys began flush on Monday so finish out this week of flush and one more week of flush after that an they will get the chop too! But stay stunned for next week , we’re not done yet so keep them eyes peeled!! Y’all have an amazing productive day as well as a great week ! Peace love an positive vibes to everybody Cheers 😶‍🌫️💨💨💨💨💨🤙🏻

Day 50 Flower (Day 92) Today is the first day of week eight of flower, and we're in overtime. According to the breeder, last week was supposed to be the final week of flower since this is a fast strain with Ruderalis in it, but the girls are still far from done. The right girl is still mostly clear, and the left girl has a bunch of fattening up to do before I'll even look at her trichomes. All I did today was to give the right girl 3 liters of pH 6.3 water. I did some bud photography as well, but I haven't processed those images yet. Day 51 Flower (Day 93) I'm starting to see a fade in some of the right girl leaves but nothing major yet. I gave her 3 liters of pH 6.1 water and called it a day. Tomorrow I have to hook up her drip irrigation system so I have a few days to tweak it before I'm off on vacation. Day 52 Flower (Day 94) Today, I finally got around to hooking up the drip irrigation system to the right girl. Setting it up was straightforward - put four drip emitters around the plant, run a hose from the emitters to a 120 l water barrel, connect the hose to a pump, set up how much water you want per day, done. For a long time, the right girl has been drinking 3 liters of water per day. One filling in my small 3-liter watering can. I figured, though, that I should first check if the can is 3 liters in the first place, so I filled it up and then measured how much water there was in it. It turns out that the can only contain 2.5 liters. Chinese quality... Anyway, no big deal when I've been hand-watering, but great to know now, as the system would have overwatered otherwise. I set up the system to irrigate 2.4 liters per day, slightly less than currently, but I think she will soon start to drink less as she matures and starts fading. In any case, I rather come home to a slightly thirsty plant than to one that has drowned. It's a bit clunky to use a timer-based drip system as it doesn't adapt to the plant. That's why I'm currently designing and building my own system, based on a Raspberry Pi, which irrigates based on moisture levels in the soil. The aim is to maintain the perfect soil moisture in each pot, no matter how much each girl drinks. This concept s precisely how a low-tech Blumat system work, and I even have one of those, but I can't use it for practical reasons, so I'm "stuck" at building my own hi-tech solution instead ;) The left girl in the small tent is just doing her thing, slowly fattening up. She is foxtailing hard, though, but I'm not surprised, considering all the stress she went through. No big deal, I'll turn those buds into a tincture instead. The flower that got burned a bit in my main tent is also foxtailing but way less. I also got delivered and installed a fire extinguisher ball in my small tent. Now each of the tents should be protected from accidental fire. I have to admit that the thought of leaving my tents up and running alone for a week makes me a wee bit nervous, so I'm taking the precautions I can. I never had any problems in the past, and I like to keep it that way. Day 53 Flower (Day 95) There isn't much to do this late in flower now when I don't even have to water anymore. Although, I am monitoring the soil moisture levels to ensure the irrigation systems work as they should. Today I also changed my Bluetooth Thermo/Hygrometers to WiFi versions since they allow me to remote monitor the tents when I'm off on vacation next week. They are currently showing the temps as high, but since I put them all the way up in the tent, it makes sense that it is hot up there. I will move them down to canopy level before I leave. I could also finalize our travel plans today after going back and forth, making plans, and changing them again for days. Next week, we're off to Iceland, where we will pick up a motorhome at the airport and then drive around as the wind and mood take us. We have been to Iceland before, but the place is so unique that we can't wait to return. It will be interesting (and a bit scary) to see how the girls will manage while I'm gone, but I believe that I've done enough to keep them safe and sound in my absence. A friend of mine can drop by our apartment and look at the girls should it be necessary, but I think they'll be alright. Day 54 Flower (Day 96) Last night, just before heading to bed, I checked the girls to ensure that the irrigation systems worked correctly. A good decision as there was a big puddle of water in the right girl's tent. Fuuuuuuu... So at 4 in the morning, I had to remove that water. Great. It turned out to be around 2.5 liters, and I guessed that the system wasn't working correctly by watering too much. Oh well, it was late, and I decided to deal with that the next day. When I opened the tent today, there was a new puddle even though I had turned off the system the night before. Huh??? I soon found the problem, though. Each drip emitter has a rubber membrane that keeps the emitter closed whenever the pump isn't running. That's in theory. In practice, however, the membrane doesn't close up properly, and water can flow through. I filled up my big 120-liter water barrel yesterday, which caused the water level to be above the level of the emitters. Considering the leaky emitters, we now had a water siphon system running, pulling water from the barrel and continuously dripping even though the pump wasn't running. Luckily I already had a low and squat 50-liter reservoir at hand, so I put the pump in that one instead, as it is lower than the emitters. That should solve that problem. I hope. (Yes, I know it is terrible with a white reservoir as it enables algae growth but it is all that I have right now and I'll throw a blanket over the reservoir before we leave.) However, from this experience, I decided to turn on the system for 24 hours, collect all water, and measure the exact amount. Honestly, I should have done that from the start already. Oh well, we live and learn. Anyway, I removed the system and taped it to the inside of a bucket, which collects the water, and tomorrow I'll measure it. Besides all this fun irrigation action, I didn't do anything else with the girls today. Day 55 Flower (Day 97) Things are looking better in the tent today. I left the irrigation system running for 24 hours, and there was no more siphoning happening after I switched to the lower water reservoir, so that's good. However, the system was still overwatering by half a liter. Not too bad on a daily basis, but as I'm gone for a week, I need it to perform better. I dialed it in by adding one more dripper but decreasing the frequency. I'm currently using five drip emitters, and they run for six minutes every 12 hours. I did three test runs (simulating a full 24 hours run cycle), and the average water volume is 2.43 liters per 24 hours. Spot on. Day 56 Flower (Day 98) The last day of the eighth week of flower, and everything is moving along nicely. A bit slow since the girls were supposed to be done already, but I'm in no real hurry. I'm off tomorrow for a week of vacation, and I hope I have taken all precautions to keep the girls safe and sound. They should be alright, but a friend will drop by in a few days to make sure. I haven't checked the trichomes in a few days, but that can wait until I return. All I did today was to check that the irrigation systems are running correctly. The cheap system for the small girl is a bit finicky to adjust, but the other system is running just fine now when I have the other water reservoir.

Day 42: Starting off Week 6 and this lady is doing awesome. I am however finding discoloration on fan leaves and I am thinking it came from the SledgeHammer flush I did. But going into the 2nd week of flower I truly couldn't be happier with the progress. She is growing well enough you can see the changes daily.. Truly an awesome strain 😎.. 12/28 More and more stacking each day.. this lady is really swelling up and she has such beautiful thick white hairs coming out right now just looking super healthy.. Finally got some of that Recharge powder 🙌 gonna start that on next watering and see how she takes to that. Will update again soon but until then stay lit growmies

🤤🤤🤤🤤🤤🤤

Rough week! Found two major hermie issues Pheno #5&2. Could be because they where the OGs and they just had a long stressful life. Really bummed because they where the biggest in the tent for sure. Have a coupoe tops with orange hairs so maybe got pollinated, all i can do is keep my eye on them! Besides that everything is looking so great had to re-train and move canopy around but still have full coverage. Getting allot more tops

Noticed the tips getting worse even though nutrients were dropped and medium has been flushed. Upped the nutrients back to full strength and tips went green again. Beats me at this point but my suspicion is a PK hungry plant ? Staggering updates for OPSEC.

La FantomOG KushV3 est juste impressionnante ! Une fertilisation qui la pousse au mieux de ses capacités. Passage en fertilisation floraison phase 2. On arrete le lacalvastress, et on diminue le booster de croissance

All three beautys has grown up to very nice and healthy looking plants.I topped them all once and did a bit of defauliation to get them ready for set in flower in a few days.

She's coming on beautifully switching to flower sometime this week so it doesn't get to crammed in there hopefully. did the final bits of tieing added the second hps last week and will begin with buddy and terpinator tomorrow thanks for reading happy growing guys

Wedding cheesecake FF by Fast Buds Germination, days: 3 Vega (18/6), days: 35 Flowering (12/12), days: 64 Total, days: 101 Ec 2.5 (Athena bloom, Fade, Cleanse, Balance) Ph 6.1 Red 100% Blue 25% UV 20% DLI ~32 T. Day: ~25C T. Night: ~21.5C Humidity: ~57% T.leaf: 24.5C Week progress. Two slow plants are still lagging behind, the video shows what a variety of phenotypes, it is very difficult to grow. I plan to harvest in a week, afraid that if I'll do not cut then buds will start to turn yellow. I probably won't keep the two lagging plants longer because the box is needed for drying. The smell is similar to sweet strawberries.

Sie haben das Umtopfen ganz gut überstanden. Eine von den dreien macht ganz gut los. Die anderen beiden haben wohl noch etwas Stress durch das Umtopfen. Alle 3 sind Samen der selben Genetik aber selbstverständlich auch verschiedene Phenotypen, sie werden auf jeden Fall unterschiedlich wachsen und aussehen. Ich finde aber No-till besser als Stecklinge und je besser der Züchter um so weniger Phenos 😉 Wir werden sehen... 😎

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Great Strain, like always from Barney! 🌱

Hello guys, almost in the end of week 6 - DAY 40 29/04/2020 Some of the plants shown really good improvement with nice stretch. Plant number 4 the one that i spotted TMV i think it was fake diagnostic, she seems fine, and on my window and balcony she doest catch to much sun so , she was back in grow tent on this week 6, and she started really fast to flower. Maximum height is 40 cm plant 2, and got her main cola lose few weeks ago because of an accident. but she recovered sending all the power the rest branches. they seem to be adapting fine to the 600W LED, i setted my LED on week 6 50 cm away from the highest plant. and now she its like 25 cm away, and with no signs of burning. i will let the LED on that position if everything stays OK. Plant number 3 still the one most small of all. And still not showing to much of the flowering, i think this one its a little bit late. But the end i think is promising. Lets see on the next Week how they are developing. My tent is becoming really tiny :D and i like that. But next time with this tent will grow maximum of 3 plants and bigger pots. Thanks for all the help given. Comments please guys. And sorry for bad pics :/