The autos continued their steady vegetative push this week, with larger 5–7 finger leaves developing and noticeable daily growth. Canopies began to fill out, giving the plants a stronger, bushier appearance compared to the previous week. On Aug 26, the pots were watered thoroughly, and beneficial nematodes were applied as a preventative step against soil pests. This was the first pest management measure of the grow. The environment stayed stable overall. Temperatures stayed warm but within a healthy range, and humidity hovered in the target veg zone. By the end of the week, the plants looked vigorous and healthy, with no signs of stress or deficiency. They are well-prepared for topping and early training in the coming weeks.

Una gioia infinita poter raccogliere questi buds ricoperti di gelo. È stata una delle genetche più belle e resinose in questa cultivar e sicuramente la coltiverò ancora una volta perché è veramente troppo soddisfacente! Gli odori dei terpeni hanno completamente invaso la casa (ed anche la strada). La prossima volta la coltiverò con una alimentazione diversa per vedere i comportamenti con una linea bio/minerale. Restate sintonizzati e ringrazio tutti voi per essere passati da qui a lasciare il vostro like ❤️ Ricordati di seguirmi su Instagram! 🌈

The last two ladies are mature and ready to cut down I’m going to wait until Easter Sunday 420 :)

Very easy to grow super frosty and super dense nuggets smells ama

Hello everyone, masters and beginner farmers! welcome back to the enchanting gardens of Peaky

Lots of growth this week. I just topped a couple days ago. The White Widow looks healthy and happy.

D43 - At the beginning of the second week of flower and the girls are loving life. Overall, I'm happy with the flat canopy, but there's one branch higher than the rest on the first girl. I've never super-cropped an auto, so I figured, why not give it a go and see what happens? It's only one branch after all and we still have weeks of flower to go. I also lightly defoliated the girls further. Nothing extreme, just cleaning up here and there. I've been lazy and haven't brewed any compost tea in a while, so I hand-watered the girls with 1.25-liters of water @ pH 6.3 with 25 ml EM-1 to boost the microbes in the soil. I'll brew some tea in a few days and feed the girls then. D45 - Minor LST. The first girl is bouncing back nicely from her super cropping. D46 - I started brewing a compost tea last night using a pretty basic recipe of worm-castings, rock dust, kelp meal, and molasses. Today each girl got 1.25-liters of tea @ pH 6.5. I set up automation to control the RH in the tent using an Inkbird controller a while ago, where the humidifier and the extraction fan are hooked up to the controller. If the RH goes above the set value, then the fan kicks in to extract the humid air, while if the RH goes below, then the humidifier starts. With a stable temperature, it works like a ghetto VPD controller where the tent is in the ideal VPD range during the 20 hours of light but still in an acceptable range during the dark hours. Controlling the environment is always a headache in a tiny tent, but this system works exceptionally well, considering it is cheap and basic. D49 - We have reached the end of the second week of flower and everything is going great in the tent. The grow is pretty much running itself now that most training is done and the watering system takes care of the irrigation. I'm still dialing in the system though.

Feeding; 2.5ml/gal - Cal-Mag .75g/gal - B1 .75g/gal - B2 1.5g/gal - Full Tilt 2ml/gal - Overdrive 11/1/24 - Start of Week 9

Harvest done after a week of flushing, the result is always satisfactory. One of my favorite strains, after the drying and curing process I'm updating my feedback! Thank you all for following the process. 💪👊

There is little to say yet. I recommend everyone to try this technique at least once. Maximize your harvest and increase your personal experience. If you want more serious proof of how mainlining works, take a look at the diaries of @Canamatoes, my teacher and mentor. More updates later, stay high🤘😁🦄

Sticky, strong smelling and starting to frost up some...

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Pistils changing color no idea how longs left now but would assume Atleast 2 weeks

1-30 She was transplanted. Just covered and sat in the pot. 2-1 So is 3 gallon pot now. 2 tbs craftblend. 1/4 cup worm poo. 1 tsp Mammoth myco. First water in the pot. 500 ml ph 6.0 7ml of activated EM 1 Little less than 1/2 tsp recharge, sicila 1.5 ml Drops 1 ml. Hindsight should have only watered 250-300ml. 2-3 Loves her new home. Stretching all the way out! Momma lost her head. 2-4 350ml water plain. 2-7 350ml 1/8tsp myko .

Middle of week 4 flower

1 plant is auto

Flowering is on! 🎄🍁

Excellent quality and quantity with this harvest! Will look to to control the stretching phase of flowering next grow.

Día 22. El estirón ha frenado. Las plantas están empezando a llenarse de flores. Se me hace extraño tener las plantas con esta forma, dado que siempre he usado cortes y LST. Se ven bastante bien, aunque cada vez que las miro, me dan ganas de cortarle alguna otra rama. Pero no quisiera mermar el rendimiento. Ya a esta altura sería más.contraproducente que otra cosa. Pero creo que debí haber sacado más ramas de las que saqué. Día 24. Fui un iluso al pensar que el crecimiento había frenado. Hoy ya las plantas tienen 5 cm más que hace dos días. Voy a tener que doblarlas si siguen a este ritmo. Tuve que hacer un video, ya que no tengo lugar para sacar fotos en contra picado. Veo que se están llenando de flores y empiezo a imaginar el resultado final. Día 26. Las plantas crecieron otros 5 cm en estos dos días. Ya estan 65 cm. Me empieza a preocupar, por lo que supongo que voy a tener que doblarlas un poco. Seguramente solamente doble las dos mas altas cruzadas entre si. Vengo ilusionado, pero sospecho que van a ser necesarias entre 7 y 8 semanas. Tuve que agregar solución, ya que había bajado mucho el nivel del depósito, y agregué un poco de bud blood. Voy a seguir usándolo hasta el momento del PK. Seguiré actualizando.

Coming to a close here with Tangie'matic. This grow has been so enriching! I decided to go outside of my comfort zone and head strong into the controversial world of topping autoflowers. Some people swear by it, others stand by their stance that topping an autoflower will destroy any chance at a decent yield by stunting the plant in its already limited life cycle. This plant has been a true partner throughout this grow process. I pushed with the top, she pushed back with buds. I pushed her again by changing her grow environment when I decided to prematurely move my grow tent into the basement. She stacked and fattened. When I traveled and let her house get too chilly, she tossed me a couple of foxtails to remind me that I need to do a better job. I responded with more environment correction and she rewarded me by finishing up beautifully.