Week 4 was great. Tried upping the water amount a bit and it worked well on 2 of the 3 plants so I´ll just stay at the same amount as before for the plant that didn´t like more water. Buds getting bigger. Also 2 of the 3 plants are gonna have a longer bloom and it shows now as you guys can see. I just hope now that they will stay healthy because I´m happy with the progress. Thanks alot again for you guys giving me tips and tricks to optimize the grow.

This week, the plant continued to develop with no major issues. However, signs of a magnesium and calcium deficiency have started to appear, which may require adjustments in the nutrient schedule with Calmag. The plant was watered once with clear, pH-adjusted water to maintain a stable environment. A mild yet distinct aroma has also begun to emerge, hinting at the plant’s unique character as it matures. Overall, growth is progressing well despite the minor nutrient deficiencies.

Baklava de The Plug Seeds es un cruce de Wedding Cake X Gelato. Hay varios Phenos (3) el mejor para mi gusto es bastante púrpura y de gran rendimiento. Soporta bien altas cargas de fertilizante como además las altas temperaturas de verano. Con el frío tiende a cambiar el color a negro/purpura con tonos de verde lima y rojizos cobres. Terpenos que recuerdan a una bolleria o panadería, toques dulces, mantequilla, frutos secos, canela, miel y el pheno Gelato sale mas a crema dulce terrosa. Hay un pheno que es bastante mas Kush y Gassy! Altos contenidos en THC y muy buenos resultados para hacer extracciones.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Because of how early I topped her, the base was very dense with foliage so I did some defoliation. You can see the four main branches (2 of which were topped) and the central FIM with its supercropping scars in the 4th pic. Still running a 1000w MH bulb at 50%. Looking at getting a SMLED/COB LED combo light to replace my cool tube and associated fans and ducting. Water level was down to 50%. I'll have to start topping up. July 04 I rearranged my setup a bit while switching the MH bulb for the HPS. I think next run I'm going to grow 2 autoflowers and stagger them and try to run a perpetual harvest. I'm going to try to stick with the HID lights if I can manage the heat effectively. It was working well when I had the 6" cooltube and the 4" carbon filter exhausting out the window, but now that the smell is more intense, I don't want any air going out except through the carbon filter. I've thought about running filter-->fan-->cooltube-->outside but they're different sizes so it would lose efficiency. I've also thought about running clean air from outside the tent through the cooltube and then outside as it was intended, but I just don't think that's going to work with the space. July 05 I noticed shiny spots on the leaves which seems to indicate I have thrips. I can't see any actual larvae even with my glasses on, but the sucked out spots are fairly distinctive and I can see small black particles that must be excrement. Temperature seems pretty good in there and humidity is ok at about 45%. I have some peppermint Dr Bronner's castile soap so I made a foliar spray of insecticidal soap by just pouring a few drops into a big spray bottle. Just worried about when to spray so I don't burn the plant. Just checked the reservoir and temp is 26c, PPM 860, Ph 6.5. I'm going to add plain water and try to bring the Ph back down to 5.5. It's possible that what I'm seeing is due to the Ph being too high. I'm going to start checking the Ph at least every couple days. It's annoying to unclip all the training wires every time I open the reservoir. It's also not very convenient to fill it. It's at an awkward height. I like the 5 gallon bucket because it was easy to set up and understand but I might be better off with a different type of reservoir. 3:45pm I just sprayed the underside of one section of leaves, I'll see how that section does under the lights. My concern is that if I spray under the lights, it will burn, but if I spray at night, it could cause mold. Hopefully I can get away with spraying all the leaves once or twice a week and it will go away. July 06 She looks ok but I'm thinking it might be a Ph issue. Ph was 6.2 so I decided to just pitch the water and start again. I changed my method so I'm mixing the nutrients in the actual bucket the plant lives in and testing it there, instead of mixing in another bucket and pouring it into the DWC. Ph is now 5.7 and PPM 700. July 08 just noticed that RH is 70% when the lights come on. I feel like I'm going to need a solution for overnight humidity. Maybe dry rid bags. Rh is 60% after a couple hours. Leaf, solution, and substrate temp down to 24 which is nice. Ph is back up to 6.3 again! I pH downed some tap water to 4 and added that to the reservoir. July 09 Pistils just started coming out :) The Ph is still back up to 6.3 again but PPM is 750. I'm going to change the res again and lower the nutrient content a bit.

Did some more LST on her and she liked it. Looks Like nothing happened to her. She's showing First signs of flowering Like getting pistols and Stretching a little Bit. I Hope she'll get bigger soon. Stay tuned 🤙🏽

Tag 81: Es wurde geerntet, da die nächste Tage nur noch Regen angesagt ist und ich keine weitere Ertragssteigerung erwarte und die Schimmelgefahr da ist. Bisher habe ich grob wettrimmed und lasse sie nun 14 Tage in der Garagen trocknen. Update 29g trocken

day of cleaning and preparing the next run , excited to grt this auto run going for divine.seeds aswell

Week 5 and the deficiency is gone. The feeding schedule is 2 times a day. I used the scrog very close to train the plants. I will keep it low but not that low. I need to be able to get in and hand water my girls. Keep it Green

Last day Light for the Lady.

All is going well and are now in pre flower.

PurplePunchOG Auto Week 4 What up Grow Fam. Weekly update on these 3 little ladies. I did a defoliation and topped the bigger one in the back but over all seems to be going smoothly. I'll start introducing nutes General Hydroponics 3 part flora series with some calmag this week and see how they handle it. All in all been a great week. Happy Growing

Week 5 flower. This one just grows slow. Slowly filling in. Very frosty, but not a nice smell. Hopefully the flowers end up tight, hard, and smelling great. Lights are at 12-14” from canopy, and plants are taking it well. Blumat drip still working, and hand watering once a week with Gaia Soluble Seaweed Extract 0-0-17. Not going to top dress anymore as these 30 gallon pots have lots to offer still and this plant is using nothing really. Thanks for views!

😍😍😍 its that time again already!!!! the flowers are coming on very nicely. I did the yoga session and get all the under canopy trimmed up. So tricky without being able to get to the back middle from the front but well worth the effort. I should film it one of the times hahaha only have to do it once a harvest but damn. anyways.......I made my own co2 mix this time, had enough lol all it is, is 2 cups of sugar and some yeast lol no need to pay $28 a batch for it, man i feel silly now. got the co2 in there and started using a new terp and resin enhancer called, well, resin lol this stuff smells even better than terpinator some how lol definetely excited to smell and taste the results. Continued with the microbiable mass every 2nd week and also got rid of the pully things i had and did a hard zip tie to the beams so i could raise the light up another 4-5 inches. This should help the outer perameter get some extra light it was not before. All in all, i'm very happy!!! the scrog is even better than last run with almost the whole tent absolutey packed. though there is still some room for improvement of course! left is SLIGHTLY more bushy canopy up this run to see if i can increase the yeilds with a new strategy. Thanks guys!!! next week i should start having some beautiful flower pics

I think I over did it on the numbers big

สุดยอดมากสายพันธุ์นี้ ฉันรักมัน ฉันจะกลับมาทำให้ดีขึ้นกับรอบต่อไป แน่นอนเก็บโคลนไว้

Week 5 – Beast Mode & The Big Flip! This week I set up a timelapse to capture just how vigorous these ladies really are — and it didn’t disappoint! 🌱 The tallest Lemon Cherry Runtz even surpassed the Papaytons, though to be fair she’s sitting right in the sweet spot in the center of the tent. I also bumped up the light a bit, and the response was instant. Big thanks to SANlight, AC Infinity, and Autopots for keeping this jungle happy. Some of the girls figured out that there’s basically an endless water source waiting under their pots, and in search of it they threw down insane root systems. You can tell they went into full-on beast mode. This time I’ve been home for almost a month straight, so I could keep a daily eye on the propagation tent. It paid off: the first clones of all four strains rooted within 6–10 days! They’ll be kept safe until we find out which phenos are the real winners. Before flipping them to 12/12 on 19.08, I pulled each cultivar out, gave them a clean topping, and prepped them for flower. Since they were so big already, I skipped cloning the tops and went for side branches instead — they root faster, which is crucial in summer when cloning isn’t the easiest game in my setup.-äpl-.lp0üß0 Meanwhile, the moms I sent to the flower tent are already flashing their first pistils — can’t wait to show you how those develop. The rest will have to stay in the 4x3 for now until the flower run is done in 3–4 weeks, so I went hard on them: lollipopped, defoliated, topped — the works. For now, the goal is to keep them compact and healthy until it’s their time to shine. The real fun is just beginning… stay tuned for flower! 🔥💚

ESPAÑOL: Hola chicos, espero se encuentren muy bien! Esta semana las chicas han mejorado un poco, se detuvo el amarillamiento, descubri que Cal-Max de Grotek interfiere en la composicion de la linea hesi por lo que las chicas sufren un bloqueo de nutrientes, dejé de utilizarlo y las chicas han continuado con su desarrollo relativamente normal. ya estan en su 5ta semana de floracion, Tutankhamon ha producido una cantidad gigantesca de resina, Lennon esta engordando a toda velocidad y aun le quedan 6 semanas aprox. Anubis a pesar de su color intenso amarillo palido esta engordando bastante bien y su produccion de resina es inimaginable y ya solo que quedan 2 semanas y media aprox. espero que a partir de ahora ya no surjan mas problemas y que las chicas terminen su floracion de la mejor manera. esto es todo por esta semana chicos, espero que se encuentren muy bien ! INGLES: Hello guys, I hope you are very well! This week the girls have improved a little, the yellowing stopped, I discovered that Cal-Max from Grotek interferes with the composition of the hesi line so the girls suffer a nutrient lock, I stopped using it and the girls have continued with their relatively normal development. They are already in their 5th week of flowering, Tutankhamun has produced a gigantic amount of resin, Lennon is gaining weight at full speed and he still has about 6 weeks left. Anubis, despite its intense pale yellow color, is gaining weight quite well and its resin production is unimaginable and there are only about 2 and a half weeks left. I hope that from now on no more problems arise and that the girls finish their flowering in the best way. This is it for this week guys, I hope you are doing well!

Hey guys :-) First of all I have to say that all 5 strains I have in the tent from Amsterdam Genetic are beautiful genetics 👍 . This week they were repotted into 8L fabric pots. When repotting, 2 g of Green House Powder Feeding Bio line were added per liter of substrate :-) . That's enough until the first flowers start 👍. Watering was done twice this week with 0.8 l each. Otherwise everything was cleaned and checked and fresh osmosis water was mixed. Stay healthy 🙏🏻💚 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://shop.greenhousefeeding.com/us/ 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : https://www.amsterdamgenetics.com/product/skyrocket-feminized-cannabis-seeds/ Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.4 MadeInGermany