Last week. 9 weeks have pass now 48h of darkeness and then chop chop..

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Continue LST. Fed with a nutrient balance compost tea.

Day 15: 👍🏾 Note: Plant closer to plant slower growth than the other. Day 18: 👍🏾 Day 21: 👍🏾 Note: Electric out for 2 days due to bad storm therefore plants are on balcony. They love their natural habitat.

Been productive week for this little lass, I believe she's still stunted but she's picked up immensely, maybe a quart from this, but it's medicine at the end so I'll be happy 😊, happy growing and stay green ✌️ 💚 👌 😊

Primera semana de floracion recuperan su color verde agrego estimulador de floracion de bac , y mantengo grow de bacpara continuar alimentandolas en este proceso de cambio de luces e ire quitando las dosis hasta solo enfocar en florecer con toda la fuerza disculpen mi falta de fotos las perdi todas

Impressionante la velocità di fioritura delle dutchfem autofiorenti! Sono nel periodo di fioritura inoltrata e si stanno riempiendo tutte di infiorescenze dure, appiccicose e molto resinose e profumate, in giardino sembra di essere in un negozio di frutta fresca! Sfiorano il metro e cinquanta centimetri di altezza alcune e la maggior parte sembrano XL perché le lunghe diramazioni si stanno riempiendo tutte in ogni parte delle piante. Purtroppo il clima al centro Italia è un po' sfasato e ogni giorno alterna caldo umido forte con pioggie fresche brevi e poi ritorna il sole. Gli afidi stanno provando con tutte le loro forze a superare la protezione dell'olio di piretro, sono sotto le foglie gli afidi ma non stanno minimamente indebolendo il vigore delle piante anzi sembrano fiorire più aggressive e la resina inizia ad incollare ogni insetto parassita che sta tentando di attaccare le mie piante. Biologicamente queste varietà sono impeccabili e si adattano ai cambiamenti climatici e ai nuovi insetti parassiti resistono. Qualche lepidottero ha iniziato a mangiare qualche foglia ed infiorescenze ma non è un problema perché manualmente ogni giorno dedico un ora a rimuovere i bruchi. Dai video e foto si vede che non si scherza con queste varietà! Un abbraccio a tutti e forza Dutchfem auto in Italia!

SO UPDATE GANG!!!! The girls are looking great, So far so good! The grow setup is taking them to levels beyond what i could have done on my own But like i said before its a learning process so im fine tuning things and we getting there lets GOOOO!!!!!

So this week after I gave it under nutrients I apparently gave it too much nitrogen while having a potassium deficiency. Shiney dark leaves, So i fixed that, but some didn't bounce back, and I tried nitrogen. I think they are doing pretty good considering everything I've put them through SO FAR. lol. Nutrients are NPK Raw's total lineup, follow their instructions at first, Fastbuds adjustments as of this week.

Getting very excited about this process! First time mainlining and it is great! Also love the advanced nutrients nuts, first time trying them!

Inicia floración en su primera semana 23 - 29 agosto

Branches are starting to need support. No issues yet this week or changes to note. Hoping to get at least 6 ounces dry from this plant, that would be a huge win.

3 in the 1 gallons are starting to take shape and swell a little bit. I like growin autos in little pots.

Flowering day 3 since time change to 12 / 12 h Hey guys . My cutting heating broke 3-4 weeks ago, which was fixed immediately and everything went wonderfully. Now, 3 days ago, the central heating broke down in the entire room, so that at night I only have temperatures of around 13 degrees :-( . One or the other lady is slowly starting to see a phosphorus blockage, as this can no longer be absorbed at temperatures below 15 degrees, like many other nutrients :-( . A friend looked at the heating yesterday and came to the conclusion that a real company had to do it, which is by no means possible for a stranger to enter my room. I quickly ordered heating mats for all the boxes so that the ladies could at least get warm feet. That's all I have this winter full can do . Let's hope everything will be fine 🙏🏻. As soon as the heating mats are attached in the coming days, I will post them in the pictures. Now for the update. Despite the severe cold, the ladies are still doing well and have made good progres . The ladies only had to be watered once this week, each time with 1 l, because it absorbs the water very slowly because of the cold and I don't want to overwater it. I took every single plant in the pot to take a close look at their roots. These are snow white and look very healthy 🙏🏻. It was also the last time neem oil sprayed so that the last trips are finally gone. Otherwise everything was checked and a lot of planning was done, like I do with the heating mats. Since a friend had the same problem in one winter and the heating mats got 10 degrees plus on the pots, I am very confident that it will at least bring something for the last cold month 😃 🙏🏻. I've started flowering even if some are still a bit small, but otherwise they'll get too wide from training and end up taking up space :-) until then have fun and stay healthy 🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : https://www.exoticseed.eu/ Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.4 MadeInGermany

Total de Dias 15 - 17/08/2021 / Inicio da 3º semana Total de Dias 16 - 18/08/2021 Total de Dias 17 - 19/08/2021 / Rega Total de Dias 18 - 20/08/2021 Total de Dias 19 - 21/08/2021 / Rega com 2ml de calmag apenas por prevenção Total de Dias 20 - 22/08/2021 Total de Dias 21 - 23/08/2021 / Rega

10.08. Wedding Cake 1# Day 107# Wedding Cake 2# Day 101# The plants are progressing well, the day before yesterday was the end of their 15th week Both plants went into preflower and 2 days ago they got a dose of bloom and half a dose of boost, and this morning they got bloom again I managed to get 2# out, but she is still constantly thirsty, and now I water her every day, but I managed to satiate her with food, in the previous days was given double doses of tnt and full doses of cal-mag. Stay High and Keep Growing!!!

Very busy have some white Dynamite seedlings started for hopefully more feminised strains. My dream is to open a seed company and become the best seed supplier! Thanks for the views. Seeds should be on eBay by tommorow (regular ones)

hello weed friends!welcome back to Peaky's Enchanted Gardens!Our beautiful girls are in excellent health and everything seems to be going well! Meanwhile they continue their phase of fattening the buds and sweating of trichomes and to help their development we have added PK Stay up to date my friends that soon I will show you a lot of glue😜🤪😎🍭