2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Striding into week 8, the buds are just stacking on top of each other and I think she is going to yeild pretty big! Just trimming leaves to expose lower budsites as growth continues. She is almost too big for me to get out of my tent without snapping a branch or 2 off so the coming pictures might be not as well lit or under Burple lights :( . Sorry! I have started to reduce the amount of Grow I feed the plant and increasing my bloom nute up to 2.5m/L this week. Flowers are starting to frost up just a little bit and should see some great progression over the next week I hope! Will update with more Pictures later in the week! End of week edit: She has come out of the tent for the last time until harvest now, buds are really starting to pack on some serious weight and the branches are resting on the side of the tent, next grow I will be using a net to support the branches. Other than things going very well this week there isn;t much else to say other than, another 4 weeks seems optimistic at the moment for harvest. I think it may be 5-6 weeks, what do you guys think? Happy growing homies!

Última semana. Hice el primer lavado con flawless finish circulando durante unas 12hs. Luego ya puse agua y quedó circulando en proceso de lavado. Es el final y ya estoy ansioso. Esperaba tener listos los esquejes de la próxima camada, pero aún no tengo todos. Así que voy a ver cuantos pongo la próxima vez. Una planta quedó un poco atrasada respecto del resto. Así que voy a ver si la dejo una semana más cuando haya cortado. De todos modos no me preocupa, ya que era la planta que dejé sin guiar ni cortar y era el esqueje más nuevo de todos. Seguiré subiendo algunas fotos por si hay cambios, aunque no creo que haya mucha modificación. Día 47. Las flores estan muy duras. Parece que va a ser interesante la cosecha. En pocos días estaré cortando.

Last week of veg

9/17 - Day 30 Flowering! Both shooting pistils with Mace pulling ahead of GG's schedule even more. GG started showing what appears to be a P deficiency on largest fan leaves. Took it as a sign to feed for BAS nutrient tea and bottom feeding some Buddha Bloom. Mace shows some mag def, increased calmag. 9/18 Added slight LST to GG, mostly to guide the main cola/stalk away from growing straight into the light. Elevated blumat res to increase warer rate. 9/23 - Day 36 Humming along! Added pics.

🗓️ Week 8 complete – 4th week of flowering A few days late with the update – been a busy week – but Seriotica is doing just fine 🌱 I managed to bring the drain EC down from 2.4 to 1.8. A couple of leaves showed minor signs of overfeeding, but overall she handled it really well 👍 📏 Current height – Seriotica: 78 cm Stretch is definitely over now – holding steady. And the best part: Frost is coming ❄️✨ Seriotica is starting to develop a promising trichome layer already – looking like she’s gearing up for a strong finish!

Hello to all you growers!!😁 its fist week of blooming my plants.girls strech very nice.the space is full..im soo exited to see buds in the future.watering every second day .the girls are very thirsty🙂

day78: watered 6.4ph day80:watered 6.5ph day83:watered 6.1ph day85: watered 6.2ph give ζamensia a try, genetics are ON point.

Day 71 - Starting week 11 off with a bang and love the look and smell of this girl. She’s really starting to fatten up and and need her to start filling in more. Thinking about 2-3 weeks max before she’s ready to chop and looking forward to it. Gave her the first of her week 11 feedings today and might cut her off to just water starting week 12. Day 72 - No big changes or updates. She’s fattening up and filling in beautifully. Will most likely start flushing after the end of this week. Day 73 - Gave her a second feeding today and next one will be Saturday. All is looking great and thinking she’s in track to be chopped at the end of week 13 but will keep and eye on her. Still some clear trichs but a lot of milky in there. She looks gorgeous! Day 75 - Looks great and on track for a chop at the end of week 13. Gave her the last feeding today and will water with straight water and Sledgehammer from here on out to start flushing her out. Day 76 - Closing in on the end of week 11 and she’s just as pretty as ever. Gave her some water today as she seemed to be screaming for it. Will probably do one more week of feedings then flush her with sledgehammer during week 13 and she should be ready for chopping. Day 77 - Ending week 11 and looks great. Have a few leaves showing deficiencies but not sure if it’s because she’s coming to the end of her life or somethings missing. Runoff pH was a little high so worked to correct that today, we’ll see if that helps. If not, she’s only got 2 weeks left.

SuperSativaSeedClub PurplePunchOG Auto Week 6 Weekly update for these three little ladies. They still haven't gone into the flower stage and for an Autoflower is unusual but still no complaints. Maybe they're waiting for another week. Over all health I'd say is good, I did start adding nutes this week at half strength 0.5tsp per gallon. I use the 3 part Flora series from General Hydroponics which seems to do the trick. All in all Happy Growing.

Great week, harvested the Candy Kushs and the remaining girls are doing really well. I've stopped feeding them and just giving water now as I am away for a few weeks and wanted to keep it simple for my partner who is looking after them while I am away. The fastberry can probably be harvested already but decided to leave her longer and hopefully give buds more time to fatten up. The Bubble Kush is looking amazing and the buds are growing and gaps between buds getting smaller, she looks like she can do with a few more weeks, so she'll be perfect when I am back hopefully. This will be my last update until then probably.

Chopped on day 85, nice easy harvest, drying up now

Day 71 - Starting week 11 today and gave her a feeding of nutes. She was pretty thirsty and took almost 2 full gallons of water. I did remove a few more fan leaves today and did check the pH runoff and came down a little bit, sitting right at 7.0. I’m thinking she’s down to the last couple weeks so might give her one more feeding of nutes then flush her out and just give water till the end. Day 72 - She’s still fattening up more each day and enough that I can notice the difference every time I look at her. I did notice a few leaves towards the top that are starting to fade to an off color so hopefully she’ll hold out and continue to fatten until harvest but I’ll keep an eye on her. Day 73 - Nothing new to report today, just pulled her out to tuck some leaves and check the trichs. Still quite a bit of clear but some milky in there. Most of the pistils are starting to turn orangish/red. She might be getting down to the last two weeks or so but I’ll let the trichs tell me when she’s ready. Definitely won’t be giving her anymore nutrients, just straight water from here on out. Day 74 - Nothing new again today, she’s looking and smelling great. I think two weeks tops left. Pistils are almost all shriveled up and an orangish/red color and mostly milky but still a few clear in there. She’ll be ready for some water tomorrow. Day 75 - Checking the trichs today, she’s actually just about ready to harvest. I gave her a good watering of plain water today and the PPM is down to about 700 right now. I’ll give her a few days to dry out then I will flush her with foxfarm’s Sledgehammer and wait for her to tell me she’s ready.

I am very happy with the results of this growth cycle. I have improved my techniques for growing plants (LST and topping) and methods for stopping mite infestation. I didn't get a great harvest(not too much big flowers), but I like the result, nice compact and very good smelling buds.I make a wet trimming process and after this I tried for the first time to wash the buds before the drying process, because I use the foliar products to defeat the mites and the result is excellent (the ganja tastes good when I smoke it). Next time I will try to grow bigger and denser flowers to get a bigger harvest💪💪😊. An excellent plant that I would recommend to beginners, who want to experiment without risking too much.

Hoping she stops the stretch soon as she'll be getting very close very close to the light if she doesn't. Very happy with how the canopy and defo turned out. Still stretching, OH LAWD. She was 25 cm away from the light, that's lightburn just waiting to happen. Got the two tents in series now with all the equipment in the larger tent so this light can literally kiss the roof, which was very much needed. Canopy was looking a little dense in parts and the corners had nothing at all so I fixed that. This really is turning into a very nice run for me. Last defo day 21, I'll have the choice between a 45 and 30 cm deep canopy it seems. I think I fucked up the defo, I think I took way too much fan leaves. I left 3 fan leaves at least to the top of each main.

All in all this grow was super easy. I couldve done some things to make it better but she pretty much did all the work. This strain would be perfect for a SOG style grow because it produces mostly a singular main cola nug that's pretty huge and then the bottom nugs themselves are also decently chunky. 4/22 chopped the lower half the plant. Wet it weighs 50grams. Still have the main cola in a dark room for another day or so before im gonna chop. 4/26 bottom half of plant dried in 4 days. 5/2 top cola dried in 5 days. Total weight is less than 20 grams at 19.4 5/12. Ten days of cure and shes looking even better. Spongey thick nugs. Dark red almost brown Pistils with pyramid shaped nugs. I also wanted to not how fast and easy this grow actually was. She has so much amber I probably couldve chopped her 2 weeks previous. 3 weeks veg and 5 weeks flowering and some would chop. Review. Growing 9/10 for ease of growth Super fast and simple. You can Fuck up and get fat buds lol High: 9/10 super stoney. No paranoia Yield:8/10 plant a super short. Like less than a foot. Can probably yield around a zip roughly Bag appeal: 7/10after cure buds look good. Before harvest buds look fantastic as well