2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Getting even blacker, my black girl

-Strain: Purple Lemonade - Fast Buds -Tent: 5x5 Gorilla Grow Tent -Lights: Budget LED Grow Lights 2 x 250 Watt LED Full Spec/Red Spec mixed boards -Light Cycle: 18/6 -Soil: Fox Farm -Air Circulation: AC Infinity Cloudline T4 Inline Duct Fan WECLOME BACK GROWMIES! Week 8 here with our STRAWBERRY NUGGETS by MEPHISTO January 21, 2020 (DAY 59) - Hey there growmies welcome to week number 9 we have great progress finally the flowering is very apparent and its making me so happy, she is stacking and starting to frost.

The stretch is slowing down and the buds are starting. The one I topped has lots of bud sites 👍 we will see witch has a better yield. It has a fruity smell with a diesel after smell. 🔥🔥

.

NICE BLACK-OUT FADE TIPS BURN HERE & THERE CUD AVE TAKEN HER XTRA MORE WK BUT THE OUTDOOR WEATHER WAS TOO RISKY!!

Run two with pineapple muffins! And my cure from run one is smelling absolutely delicious. This first Pheno of pineapple muffins is showing the same traits of my Pheno 1 first run. I’m going to cross breed this Pheno with humboldt headband or possibly grape odder popz depending on how those Pheno hunts are looking as well.

10 semanas desde germinación día 11 de abril hasta corte viernes 25 de junio (día 69 corte) cultivada en maceta de 11 litros con tierra plagron all mix, inicio de floración en semana 5, día 20 de mayo

Olá pessoal, nova semana, Purple Punch está uma verdadeira árvore 🌲 esta semana voltou a surpreender-me cresceu 15cm de altura está com 1,65m e cada vez mais ramificada, as ramas laterais querem ir ao topo como a haste principal, tive que adicionar uns tutores para suportar o peso das ramas, esta semana adicionei growth-c e alga-c, tudo vai muito bem, Fico maravilhado a cada dia que passa. 🤩 🌲🌱🍀 Bons cultivos companheiros

Because I used 2/3rds of the fresh plant on dry ice extraction and because I did it solo, there are no official weights for the CBD harvest. I will weigh the 1/3rd still hanging and report on that and my estimate of what the total weight would've been. Because of the size of the colas, the number of branches and such, I'm going to guess that it was very close to 3oz or slightly over as it was larger than the BHB. Will definitely grow again. Very nice genetics! Harvested the CBD at the recommended moment when the trichomes were as close to all cloudy as possible. I believe it was around 90%. Very strong effects that are not psychoactive. Highly recommended.

Bonsoir à tous Voilà encore de nouvelles photos et vidéos que je viens juste de faire, mes fifilles grandissent bien encore une bonne semaine et elles passent en floraison je pense vivement 😜 j'ai hâte de là sentir et Surtout de la goûter 😋😋😋 je vous dis à bientôt bye bye

Hallo Ihr lieben 💚 Woche 2. hat bekommen, die Damen wurden in Ihre 20l Töpfe umgezogen und haben inzwischen wieder Fuß gefasst. Bald werden die kleinen getoppt und dann wird es auch endlich wieder etwas spannender. Ich finde die ersten Wochen vergehen immer sehr langsam. Genießt das tolle Wetter und habt einen guten Start ins Wochenende. ☀️

Hi all the happy people here in GrowDiaries. And hello to you who read my diary, I just want to say that I am pleased that you have chosen to check in with me and in my diary. I do this for myself and for a steady flow of my medicine. Everything you read and see in my diary is 100% honest and I will never distort or beautify anything here. I document my crops so that I can learn from my mistakes and also to look back at those different crops. I try to update with pictures every day and with text if something special has happened in the garden. This is my strainhunt for the best medicine and the beginning of my journey with cannabis and the cultivation of it. This is my second feminised cultivation ever. First, I'm just going to say something about my grow room. The room is 2.14 meters by 1.7 meters and has a ceiling height of 2 meters. It provides a floor area of ​​3.6 square meters. I use a 54 Watt Lightwawe T5 for germination and 2 Pcs 400 Watt HPS lamps for bloom. I have a channel fan that replaces the room air about 40 times an hour to get a comfortable environment in the room, the air enters a fresh air intake from the outside. The air is purified through a carbon filter to then leave the room to the rest of the basement. Then I use that heat to heat the rest of the basement. I will use 15 liter Autopots to grow with and a 100 liter water tank that supplies the pots of water and nutrition. I will grow completely organically in soil and will watercure my buds to get the best possible medicine for me. Medical Mass is a new medical cannabis strain with a high CBD content and a low THC level that provides good medical properties without a strong psychoactive effect. This is a mostly indica plant that contains 40% sativa and 60% indica. Critical Mass and CBD rich strain were crossed to create this amazing valuable variety. The plant suits both for indoors and outdoors, for beginner and experienced growers. The strain grows up to 60 - 100 cm tall and produces good yields for such height of about 500 - 550 gr/m2. The plant demonstrates great results even in small spaces. The flowering time takes only 7-8 weeks and the harvest comes in late September. The smoke brings very strong, intense flavors with sweet notes of honey and skunk and an intense pleasant relaxation. Medical Mass is the great choice for each medical user due its small height, ease of growing, fast flowering and of course great medical potency. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 2017-09-27. Week 3 day 2. The girls are doing awesome and has grown a lot. Girl Nr 1 is 18 cm high, Nr 2 is 14 and Nr 3 is 15 cm. This week i going to start defoliate, give nutes and some LST.

Going well;))

Easy for grow, If you haven't tried this strain. You should hurry and buy the seeds. A link for buy 👉🏻( https://2fast4buds.com/ )