July 9th - fish poop applied for breakfast - with all the rain, i applied Kelp Meal to the surface area - fed 2 gallons of Nutes/water -Weather can do what it wants now June 10th, - looking at the leaf formations on the end of the Branches, and I’m sure there will be Pistiles showing in about a week. The bud sites are starting to take shape and cluster. I’ll grab some shots in 4 days and see where its going - foliar spray for breakfast, thats a good feeding in the last 24 hrs - next meal posted above - 2 weeks past the Solstice, the plants will start moving into Flower very shortly, if not already. Days are Shorter, lets grow some Buds July 11th, - another whole day of Rain, so 1-2” (~ 1.5 gal) of Compost was added to the entire Soil surface. A first for me, thats for sure… all in the name of the science in the soil - before adding compost, I stood on the tomato cage, to fully re-sink it into the ground; top rim is 29” legit July 13th - foliar spray in the late afternoon - plant is becoming a Bush - yep, in a moment of weakness lol, I took a few leaves off, clones were gone, had to make it right… call it LST - you can see the branches are making use of the Cage, for support of the lower Topped branches.

Happy 420!😁😁 She began week two and she looks healthy💪 I was think about some lst or topping but i decide let her grow natural without any stress✊

If you are looking for yield then green gelato is most certainly the one you're looking for. If controlled right you can easy get 10oz per plant on these!

This gurl is done! She smells and feels sticky buds are not huge how I wanted but I take into consideration I use very little fertilizers and she started turning yellow and leaves dying!

Week 2 of veg and the plants are looking healthy and growing. It took the plants a good few days to get over the transplant shock. I understand now why you don't transplant auto-flowering plants. Day 13 of Veg: The plants are looking healthy and producing new growth.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Alright so last week is here, finally... After smelling this girl you literally want to bite the plant and eat it 🤗🍭🍬, this thing smells like candy and all kind of sweet fruit loops and things, its a world of happiness and unicorns in a plant! Basically she didn't get that big but this was expected as the plant only gets some hours of direct sunlight, maybe 6 or 7 + we had several weeks of rain and now we have an heatwave (this fuc*ing weather is amazing) the good news is that the worms learned their lesson and didn't appear again (they know this hood is mine now!) For the rest, cant wait to harvest and start another grow, as mentioned in my previous balcony grow i would like to put around 6 or something like that, even if i get only 10-15g per plant its still worth it, sunlight is free and its just a little balcony, they dont disturb nobody there

Day 8: Watered the plants 0.25L each plant with nuts 1 seedling was burn because of too much humidity. One seed already germinated ready to replace it. Lights at 40% Day 11: Watered the plants 0.25L each plant with nuts

Looking and smelling the goods , a week of flush to come and then I can smoke it all , she’s my fave lil plant ever , thanks heaps to fast buds … it’s perfect

Harvest day 67 since time switch to 12 / 12 h Hey guys :-) Finally it's time 💚 The lady is done the large leaves have been removed and hung upside down to dry in the dark drying room. You can now stay there for 13-15 days at a temperature of 16-18 degrees and 55-59% humidity. After 13-15 days it is neatly trimmed by hand and placed in jars with boveda packs 62. After 4 weeks Boveda 58% come in and are ready for testing ;-). After everything has been cut cleanly, the last update comes with the smoke report and the finished pictures. Let's get to the plant 💚. Unfortunately, the rating system at Growdiaries is a bit strange because I have to give the stars before the Smoke Report and in the end it might have led to more stars in terms of taste. . The smell is great I'm curious about the taste 💚 Have fun and stay healthy 💚🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 ‘Powered by GreenHouse Feeding’ Copy the link for 10% off all Nutrients 👇🏼 http://shop.greenhousefeeding.com/ affiliate/madelngermany_passiongrower/ 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 Water 💧 💧💧 Osmosis water mixed with Cal/Mag (24 hours stale that the chlorine evaporates) to 290 ppm and Ph with Ph- to 5.8 - 6.4 MadeInGermany

This is the 8th week. Thursday will be 9 weeks of 12/12. Some trichs are amber. They just added a good bunch of girth over the past few days, but most of the pistils are red now. The rest of them have been dropping leaves consistenly but the canopy remains full so they must be putting out more leaves. I just flushed to 80% runoff and found out that I had 1200ppm. I just got an ec meter but my last reading said closer to 600 so idk what happened there. I flushed until I had runoff ppm of about 150. Now they're back in the grow tent. I don't want to keep assaulting them with my microscope. I think they're ready. Just gonna let them settle another day or two to dry out a bit. I took one small branch off the small Wedding Cake plant on Thursday and I've just been watching it dry on my counter. It's been 5 days and the stem snaps but still leaves a fiber connecting. I put it into a small jar with a 64RH thing. I just want to try it. It already smells amazing. The leaves on the big CBD Blue Shark plant are going really yellow and some other colours are starting to come out as well. The buds all fattened up and got more frosty again. I was worried they were foxtailing but they got more calyxes underneath the peaks and have been making more of a pyramid shape. They're really close to peak. Harvest day will be March 17 or 18. March 17 1023pm chopped and hung after lights off all day. RH was 65% before I chopped and 50% after I closed the window and cut the plants off the saturated pots. I did a wet trim to prevent mold. The buds are sticky af. Gonna hang them to dry for 7-9 days before curing in jars :) March 18 : checked the RH when I woke up and it's chilling at 45% 😎 March 19 : just checked to see how they were drying. There are seeds in every plant. RIP I'll update when I put them in jars and again a few weeks later for final review. Overall it was a good grow despite the rocky start and a good introduction to the mainline technique and my new grow space. Next grow I should have everything pretty well dialed in. March 23 They were hanging for 3 days. Rh was around 45, then spiked to 65 overnight when I forgot to turn the fans back on. Temp was high at around 26.they felt wet after 2 days, and bone dry after 4 days. The stems still didn't snap but I put them in jars anyway. A day later, jar RH was 50%. I burped all the jars and there was a smell from the new jars. I took all the weed out, washed out the jars, and did a dry trim on the weed. One of the Wedding Cake plants must have gone hermie. I didn't see any bananas and didn't even realize there were seeds until the very end. The seed pods looked just like calyxes while the plants were growing. Many of the nugs are unusable. Under the sugar leaves are just layers and layers of seeds. Some weren't hit that badly. The CBD Blue Shark is much better off but still has some seeds. I'm trying to stay positive. It's a bit of a shock though. I'm anxious to try the finished product. If the flowers at least taste good and have a good effect, all is not lost.

coming along now guna be a bushy girl can tell

8th week , 4th of flora They are tall, strong and healthy Using the same fert proportions as vegetative stage. They reached 77cm tall

Love the strain. There was heat stress, and for such much light 2x2 tent would be better. After curing: Tasty as hell grape "cold" flavour, body tranqulizing high, after few hits from the bong its a good idea to chill and watch a movie. Absolutley love the smoke in the end, from bag appeal to high :)0

After a week of curing the flavour and aroma are already good, I imagine in a few weeks it will be the best.

So first of All i have to say this strain is recommendable. It is Not a heavy gitter that is for Sure, but it delivers a pleasent high with a relaxing Note. Flowering takes a little longer then it said but that is alright. i harvested at about 5% amber coulored trichomes. I will give this strain a try i would recommend taking clones during flower, due to the exploding growth paterns afterwards. Cheers to everyone who had a look Frugo