Be happy get couple ounce off blueberryz she really stinks, very fruity and starting to get frosty. Limoncello looking amazing caked in trichs strong lemon and pine smell. Bud seem to get fatter and each time I look at her which is often she's a sexy plant.

week 9 was another great easy week she is growing like a champ I think I'm in 😍 💘.. Hoping for no hitches from here till harvest shouldnt be to hard

She’s fussy as hell, but gorgeous! Getting some purple hues now. Kind of like the crazy/hot scale. Hopefully she’s worth the pain. I flushed her out, so hopefully the bottom leaves stop yellowing so much. Still has as much as 3 weeks.

Hello Diary, In the end I can only say well done Royal Queen Seeds. I have grown a total of 4 out of 8 new hybrid F1 strains so far and all of them exceeded my expectations. Apollo F1 is no exception, although it was the smallest on the Farm, the flowers are the hardest and incredibly fragrant. During the entire cycle of growth and development, it proved to be very resistant, so that the plant looked impressive until the very end. The effect of Apollo F1 is relaxing, so I can consume it during my free time as well as when I am engaged in some creative work. It certainly removes stress and bad mood. At the end of drying, I got a little less than 50g, 49g to be exact, which is satisfactory especially because of the high quality flowers. I think I have mostly written everything that I thought was important, if anyone reading this text has any questions about Apollo F1, feel free to ask, I will be happy to answer. I would like to thank everyone for their support and comments, and especially Royal Queen Seeds for their many years of support.

👌

6/23 Due To The Two 8 Day weeks this week will only consist of five days to get me back on track. SEE LAST WEEK FOR DETAILS. I DID ANOTHER EIGHT DAY WEEK. I'LL DO THIS WEEK AS SIX DAYS. THAT WAY IM BACK ON SCHEDULE. WATERED 6/22. WENT BACK AT NIGHTCAND WATERED ANOTHER GALLON WITH 1/3 TSP Armor Si. I mixed the solution with kelp you kelp me but I dumped it out. I've mixed silica with other nutes before but I had read something earlier about bioavailability issues and I didn't want to risk it. My plants are the healthiest I've ever grown. I'm considering transplanting the large 10th planet that I'm light depping. I also need to develop my ipm and my nute schedule. Still gonna have to keep an eye onvthe water situation. Luckily I have a buddy in my corner that's co tinualkt Bern a great help. WENT BACK OVER AROUND NOON. BEAUTIFUL OUTSIDE AND THE PLANTS WERE LOVING THIS SUN. I DEFOLIATED SOME BURNED LEAVES AND MIXED A SOLUTION IF KELP ME KELP YOU THAT I PLAN TO FEED TO THE GARDEN TODAY. IM HAPPY WITH HOW THINGS ARE GOING. WE HAVE SOME RAIN AND THINDERSTORMS COMING. I NEED TO GET MY IPM UP AND RUNNING AND THE NUTES ONLINE. I ALSO NEED TO DECIDE IF IM GOING TO TRANSPLANT THE LIGHT DEP 10th PLANET. Went back over to put the girls to bed at six and if was still 90 degrees. The water from yesterday had dissipated. The tops were dust dry. I mixed two gallons of kelp you kelp me and fed it to the garden. Afterwards I pointed qbd found a couple plants that were more open to the wind were a little lighter. I know tomorrow will be very hot. Maybe quarter inch of rain. However after that it will rain (showers) off and on for days. I wanted to make sure the girls had what the needed to make it through tjis heat wave until they get that rain. I had another gallon mixed up and I'm thinking I should've given it to them. Oh well. I'd rather underwater than overwater any day. I did notice a burnt top on the plant furthest from the tarp that the sun JUST BARELY TOUCHES when the rest are in shade. I'll just raise the tarp a little. These are tiny problems. Can't upload video until tomorrow. 6/24 Good thing u gave the plants that water. I know it wasn't much but we didn't get any rain. Suppised to get a qtr in today and another qtr in tomorrow. Then rain a couple more days. It's working our really good for my watering schedule lol. Yesterday the girls got there first dose of a bottled nute. They got two gallons of kelp me kelp you (1/3 tsp per gallon) and another gallon of just phed water. I was going to do another gallon but held off due to the threat of syring rain. Didn't get it bur it looks like the plants loved the kelp. It had reached 90 yesterday. They look better today. It's 70 at 9 today. I LST the 10th planet in the 3 gallon. It's getting huge. I should start a separate diary for those three light dep plants. Its cool as hell watching this. One of the purple punches is flowering pretty good. I probably should start another diary for them. I'll be starting to feed my plants soon. Wondering if I should pinch plants again. TUCKED THE LITTLE GIRLS IN AT SIX AND SHOT A VIDEO AND TOOK SOME PUCTURES. PLANTS LOOKED PHENOMINAL! GOT HOME AND AROUND SEVEN IT JUST STARTED RORREBTIAL DOWNPOURS! THE TYPE OF RAIN THAT BREAKS STEMS. I SHOULDVE PUT MY OTHET TARP UP BUT I DIDNT. I WISH I HAD. IM A LITTLE NERVOUS. I KNOW THESE GIRLS ARE STRONG THOUGH! TOMORROW. ILL ROLL THE RARP UP AND SECURE THE BOTTOM FOR FAST ACCESS. IM ALSO GOING TO KOOK INTO FINDING A TOP FOR MY CAGE I CAN USE IN INCLIMENT WEATHER. 6/25 That little qtr in of rain we were supposed to get turned into torrential downpours and high winds. Luckily no REAL damage. If you watch the video you'll see what I mean. I'm going to havecto be cognizant moving forward. Noticed a few chunks from leaves butvplants are still healthy. I'm surprised how well these girls withstood this storm. They are certainly resilient. The 2 purple punch 1 10th planet that I'm light depping are flowering nicely now. They missed all the commotion from the storm as they were inside. It's smelling good. I'm happy. 6/26 It's what a friend would say is "maineing") out. That mistcthe plants love. I'm surprised the plants are doing as good as they have been considering the wind. Will be adding nutes soon. Need to add extra supports too. Light dep 6/27 Brief periods or torrential downpours. Light depped 3 gallon 0lants are under an overhanfcwhete the hatsxrain can't hurt them but they still get sun. They are really starting to flower now! I'm going to begin feeding with a base nute and a flower nute I think. I'm thinking about giving the light dep some open sesame or something similar. I need to set up a nute program soon but the plants ALL look so healthy! And it's been raining! I've only watered like once or twice since they've been in the big pots. I've seen zero pests aside from a chunk missing from a grasshopper. Usually by now I'd have battled half a dozen things and be battling another half dozen. However I was posing from clones. These are seeds and boy don't I see the difference!

🤙Everything goes according to plan. Blueberries show a confident growth and filling of space with green ☘️ ▪️I water every other day through a powerful sprayer with clean water 💦 without fertilizers ▪️My close landing technique in action took only 2 week, and we have 18 major growth tips on almost the same plane ▪️the lamp is still 25cm from the tops, improved the cooler mount now it is exactly in the middle under the lamp ▪️the plants are tremendously strong and dynamically developing, in this regard, I worry about the fact that they will stretch overnight and touch the hot♨️ surface of the diode. update: 18 day installation of SCROG I use tents of the 🔸SecretJardin company version 4.0; they come with a stunningly comfortable SCROG; they are elastic and allow you to adjust the crown of the 🌱 plant by installing an additional staple

8/27 I think I mightve been overwatering. It's so hard to tell when the conditions change week to week. That's Maine for ya. Bags were still heavy and plants were soaked. I tried to shake the more advanced flowering plants without sending water everywhere. That's how septoria spreads. The event horizon I treated with plant doctor looks the best it ever has. It's tile for another application on the toasted toffy. I'm seeing a few spots again. I didn't have time to take pictures or do a video. This video is from last night. I'll treat that toasted toffy again today and I'll record it here. After I see it working u may treat the others. I see some residue of k bicarb but it has pretty much eradicated the pm. I know it will always be there but these treatments seem to be working. I'll be adding beastie bloom to my line up soon. EVERYDAY I go over the buds are bigger more compact and dense. Event horizon, toasted toffy and the red runtz (I think) in the 50 are flowering to beat hell! The get bigger everytime I see them. I can smell them as soon as I round the corner. This is going to be a good year. These strainsxare high in thc. Maybe I gave up some mold resistance and a harder to grow strain but if I can make it to the end it will be fire. WENT BACK OVER AROUND 5. OBVIOUSLY IN THIS SCORCHING 80°+ day the seedling in the 10 was dry as a popcorn fart. Some other plants seemed dry when I stuck my arm down the bag but I could feel some moisture when I pushed my finger through the medium. They WILL need water but I'm not giving it to them tonight. Took a shit ton if pictures and a video this morning. I'll uploadcthat shit tomorrow. I'm trying to do a couple right now. Anyway I continued to defoliate what needs it. Flowers are swelling. I GAVE THE TOASTED TOFFY ITS 2ND TREATING OF PLANT DOCTOR. I USED A QTR GALLON OF A 4 TSP PER GALLON MIXTURE. I MADE SURE TO GET AS MUCH OF THE FOLIAGE AS POSSIBLE AND THEN I USED THE RESTCON THE MEDIUM. I SHOULDVE PROBABLY WATERED THAT ONE BUT ITS GOING TO RAIN TONIGHT. AT LEAST I HOPE IT IS. Looks like some of the pics are uploading! Yay! The plants looked the best I've ever seen them tonight so I held off watering. My wife is I'll and may need to go to the hospital. So instead of watering prematurely I can monitor the situation and make a decision in the morning. I made enough phed water for my father to go around and water everything. He is aware of the situation. Hopfully that will not be necessary. However, I don't want to over water and I feel like they didn't want it. Just going with my intuition. It's done me pretty good so far. 8/28 Luckily no hospital. It's sprinkling out. Plants are covered in water so we got SOME rain. It says we will get 0.00 but that can't be right. I watered the 10 yesterday and everything else this morning with a gallon each except the middle gmo in the 30 which got 1.5 gallons. Did not water the one in the 50 gal pot. It looks a little overwatered. Also looks like it has a slight calcium/magnesium deficiency. I'll up the calmag and i'll make sure when I do water I'll water thoroughly. Like usual toasted toffy is looking better after the treatment. The all star has been the k bicarb. I haven't seen shit for pm. Just a little residue from the k-bicarb. Took a video and some pictures. This is the fun time. I like watching the buds develop. Some of these plants were monster cropped so they are STACKED. I'll keep what I do updated. 8/29 Made an awesome video today bit it won't upload. Probably because its a little over 2 minutes. I'll do another one if it won't upload. Nope it won't even attempt to upload it now. Oh well. They flowers just looked so pretty this morning. I noticed some WPM on the event horizon in the middle. I noticed SOME on the bottom and interior of the GMO that I had already identified. I'll do another k-bicarb treatment and see how that goes. Plant Doctor seems to he doing a pretty good job on the septoria on toastedctoffy and my favorite event horizon. That plantvis THICK. it's going to be stacked and have some huge colas. I need to LST it and suppirt those giant colas and separate them a bit more. There is never any shortage of work to be done I can tell you that. I have defoliation to do on the sherb pie and the middle event horizon. It seems like crisis after crisis. Bu my plants are STRONG. 8/30 It was in the 40s at 6am. Shook off the plants. Bags are still heavy. I see some septoria climbing a bottom branch of the toasted toffy. It's foggy as hell out. Can't see a foot in front of me. Saw a LITTLE PM on the gmo I've been treating. It's just time to apply the ki bicarb. Plant Doctor is working wonders on even horizon in the back. I see some pm on the one in the middle. I think I'll treat that with plant doctor as well. I'm not sure. I messed up the video. I've got work to do. I'm waiting until I water and I'll feed again. I'm going to add beastie bloom to the lineup this time I think. Only one plant seems like it m9ght be too early for it. We'll see. I need to look back. I'll document what I do. Video won't upload. I'll try later but it's sideways WENT OVER AROUND 3PM AND EVERYTHING LOOKED FABULOUS. WELL....THE PLANT I DIDNT WATERCLAST TIME IN THE 50 WAS DROOPING SO I GAVE IT A GALLON AND A HALF. THE TEN WAS LIGHT SO I GAVE IT A GALLON. IT WASNT DROOPY BUT ITS A HUGE PLANT IN A 10GAL AND USES A LOT OF WATER. I WAS PLANNING TO GO CHECK THEM TONIGHT AND SEE IF THEY NEEDED WATER BUT EVERYTHING IS PRAYING TO THE SUN! I DONT WANT TO OVERWATER AND I BELIEVE THESE WOULD BE BEST SUITED TO BE WATERED IN TGE MORNING. ILL FEED THIS TIME AS WELL. IM CONSIDERING ANOTHER APP OF K BICARB. TOOK A FEW PICTURES AND VIDEOS BUT ILL NEED TO UPLOAD 8/31 FED AND WATERED EVERYTHING. TOASTED TOFFY DIDNT GET FED DUE TO SEPTORIA AND THE 10 ISNT FAR ENOUGH ALONG.. THE 50 WAS WATERED YESTERDAY BUT GOT THE FEED WITH A LITTLE MORE WATER THIS MORNING. I ADDED ABOUT 1/16 TSP BEASTUE BLOOM TO THE REGULAR LINEUP TO SEE HOW IT GOES. MY BUDDY TOLD ME LOTS OF PEOPLE HAVING PROBLEMS WITH PM. I WENT BACK OVER TO DEFOLIATE AND LOOK FOR PM AND I FOUND IT. ON THE GMO INTETIOR AND SOME BOTTOM BRANCHES. I DEFOLIATED WHAT WAS BAD AND THEN I SPRAYED 3 GMO'S, EVENT HORIZON IN THE MIDDLE AND SHERB PIE WITH K-BICARB. HOPFULLY THAT WILL KEEP THINGS OUT OF CONTROL. I WILL BE WATCHING AND KEEPING UP WITH FUNGACIDES. IF I FIND ONE NOT WORKING ILL SWITCH IT UP. I HAVE A FEW OPTIONS. BUDS ARE EXPLODING IN GROWTH! WINDY OUT. I SAW A SECONDARY BRANCH LIMP AND MY GEART SUNK. I LOOKED DOWN THOUGH AND SAW THAT IT JUST WAS BLOWN HATD AGAINST THE TOMATO CAGE AND SNAPPED. I REMOVED IT WITB A SIGH OF RELIEF. MY BUDDY SAYS THE SEPTORIA DOESNT LOOK TO BAD AND ITS PRETTY FAR IN FLOWER SO HE THINKS ILL BE ALRIGHT. NEVER GROWING CLONES AGAIN. ILL STICK TO SEEDS AND STEAINS MADE FOR MAINES 9/1 Forgot to write an entry. IT RAINED FOR A BIT BUT TURNED TO BRIGHT 80'S 9/2 WATERED EVERYTHING. I THINK ITS TIME FOR ANOTHER TREATMENT OF PLANT DOCTOR TODAY. The septoria is getting a little worse. The buds are swelling everyday though. I think this plant only has a month left. I just need to outrun it. So far so good. I think my watering habits are better now. It's different depending on weather. Potassium bicarb has been wonderful treating the pm on that gmo. I treated the middle event horizon with it as well. It seems to work awesome. I'm losing tons of leaves. I hope that's normal senescence. I think it is seeing as how I see SOME foliage in the woods. Its matching other diaries. The plant must shed some leaves to make these flowers that seem to double in size every time I see them! Trying to upload a couple videos but they are long so we'll see what happens. WENT BACK OVER AT NIGHT ANDCAPPLIED A LITTLE BIT OF K BICARB AND DEFOLIATED WHAT I SAW ON THAT GMO. IVE BEEN DEFOLIATING WHAT SEEMS LIKE A LOT BUT BUDS ARE SWELLING AND ITS CONGRUENT WITH THE MAJORITY OF MY DIARIES. I WISH I HAD PERFECT CONDITIONS BUT I DONT. I TREATED THE EVENTHORIZON WITH PLANT DOCTOR AGAIN. I USED APPROX A QTR GALLON OF 2 TSP/GALLON AND TRIED TO AVOID BUDS WHILE STILL GETTING THE LEAVES. I KINDA USED THE REST AS A SQUALCH SINCE I HAD WATERED THIS MORNING. THE WIND WAS STRONG. I ADDED A GARDEN STAKE AND ROPE SUPPORT TO THE TOASTED TOFFY AND ATTACHED IT RIGHT TO THE FRAME OF THE CAGE. LOOKS GOOD FOR NOW. ILL KEEP THINGS UPDATED.

Finaly, they started to build some buds.. im sorry guys for prety weak updates,.. hopefuly ll change it in close future :) 💚

Still wet, but I am very happy with the result for my first grow :) The autos grew much bigger than I expected and I underestimated the growth spurt during flower, so I had increase the setup beyond what people might see as pure budget. Anyway I can't wait to try :)

27/04/2021 (Day 42) Todo viene muy bien, las plantas están comiendo con muchas ganas y consumen unos 15L de solución diario. La preflora está comenzando! Ya se puede observar como van estirando y mostrando bastantes pelos! Los Citizen x 8 están trabajando a un 80% rondado los 500W. 28/04/2021 (Day 43) Se realizo un nuevo cambio de solución, las plantas ya están tirando pelos y los leds están al 80%, se observo un gran crecimiento radicular y las paredes del tallo son increíblemente gruesas y duras. 03/05/2021 (Day 48) Las plantas vienen con un crecimiento optimo pero la flora se está tardando demasiado en hacerse presente, se estima que es debido al estrés que tuvo en semana 3 de vege produjo un retraso de algunos días. Esta semana se incorpora Safe Roots al sistema con la finalidad de que deje la solución lo mas limpia posible. También se realizaron bastantes defoliaciones ya que no existía penetracion de luz teniendo en cuenta el tamaño que están ocupando (85% del espacio de cultivo a 30/35 cm de altura)

Week 2 - I gave up on 2 that only grew a tail, didn't make it through transplant. 3 healthy plants now! I keep hurrying them deeper in their dirt. Excited to see the progress!!

Vigorous Males, Strong Clones, and Bud Formation This week was full of action in the grow! Two out of the three males are in full bloom, and I’m actively collecting pollen. They are impressive plants, with strong structures and large pollen sacs. If everything stays on track, I believe the females will be ready for pollination in about a week. Observations: All 18 clones have successfully rooted! Some have already entered the vegetative stage and are receiving supplemental light at nightfall to keep their growth steady. The plants responded very well to the light defoliation I did earlier. Now, the buds are starting to take shape, showing great progress in the flowering phase. To optimize this stage, I spaced out the pots, ensuring better airflow and increased light penetration, which should support full and healthy flowering. This week, I plan to top off the pots with more substrate, ensuring the plants have enough space and nutrients to reach their full potential. Next Steps: Keep monitoring the males and collecting pollen at the right time. Watch the females closely to determine the ideal moment for planned pollinations. Observe the clones' growth and adjust care as needed. The grow is on the right track, and expectations for the coming days are high!

Last week of pre flower girls are doing great can’t believe they’re clones

Solo cup challenge continues to press along. Not much change, plants are in veg, growing and healthy. Still a few weeks before the flowers start so I hope to get a bit of size going before it does. Having fun and really like looking at the other entries in this contest. Check out WildeWeed, Butterstotch and BeanswithPork, silky, larimar, fireman

3 Auto's set away. Green Poison, Jealousy and Skywalker OG Not a lot to report on as such. 14 days old. Epic Attic 1.2 x 1.2 x 1.6 with 'Karma EVO 3' https://ledgrowstore.co.uk/products/karma-horticulture-240w-evo-3-0umol Ask for James, Top Bloke. 60x60x120 with Karma Stealth 1000 Strains purchased from 'The Vault' Seedbank. https://www.cannabis-seeds-store.co.uk/ Ask for George, Top Man. Pots/Trays/Substrate/Nutrients purchased from 'PeterleeHydroponics' Ask for Neil, he's a Legend!

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

This girl was a fast finisher harvested on day 46 of flower, nearly 7 full weeks.