2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

I'm a bit disappointed with the yield but overall its a great strain, smells great! My yield could have increased if all the plants were the same size. Heisenhound tastes like Master Kush, Def a good auto kush

Welcome to my Cherry Cola🍒diary. In this Diary: Seeds: [420 Fast Buds]from my growmie Tropicannibis_Todd 👊🏻😎 Media: Pro~Mix HP Open Top Grow Bag, Connect. Nutrients: Green Planet Nutrients, 2 Part Dual Fuel starter kit. RealGrowers: Recharge. Diablo nutrients: Ripping. Advanced Nutrients: Flawless Finish. ___________________________ Feeding : Mon 01Apr: 3L Monster K pH'd 6.5 Fri 05Apr: 2L Flawless Finish pH'd 6.5 ___________________________ Today Monday 08-Apr-24 is 83 Days from seed or 79 Days from Sprout🌱and the trichomes are just about right. Tomorrow morning the chopping will happen.😁 ___________________________ Thanks for stopping by, likes and comments are appreciated.👊🏻😎 Keep on growin! Keep on tokin!!! 😙💨💨💨💨💨

I water with a lot of water and I plant directly in my mixture of 70% coco, 30% perlite. Water : tap water at 280ppm. I adjust the Ph to 6. Temperature : 21°C Humidity : 60% --------------------------------------------------------- Looking for MarsHydro equipment for your crop? 🔥 You can use my promo codes! 🙏😻 3% off with "houstone3" for: TS LED Grow Light, Tent, Ventilation 5% off with "houstone5" for: FC&FC-E&SP LED Grow Lights; Grow Tent Kits https://www.mars-hydro.com/?acc=hou-stone

Welcome to week six! 🌱 Week five was a complete success – all parameters are perfectly aligned. I’ve increased the water conductivity to a solid 3000 and bumped the CO2 level up to 1200 🌿. Light intensity is now set at 1400 µmol in the center and 1000 at the edges, which will keep the plants strong in their generative state 🌞. I’ll maintain these levels until the end of the third week and then start slowly removing the large fan leaves to ensure the buds get plenty of light 💡✨. I’m also trying something new to activate the “Emerson effect” 🌄: I’m running the deep red lights for 5 minutes before sunset and for another 5 minutes after the day ends. This should give the plants a final boost in photosynthesis, helping to maximize their growth potential! Everything’s looking fantastic so far! The Black Cherry has rooted well across different experimental setups, and at the end of the grow, I’ll provide a full report on the best care techniques for both strains 🌺👌. Willkommen in Woche sechs! 🌱 Die fünfte Woche lief perfekt – alle Parameter stimmen. Ich habe die Leitfähigkeit des Wassers auf satte 3000 erhöht und den CO2-Gehalt auf 1200 angehoben 🌿. Die Lichtstärke beträgt jetzt 1400 µmol in der Mitte und 1000 am Rand, was dafür sorgen wird, dass die Pflanzen stark im generativen Zustand bleiben 🌞. Ich werde diese Werte bis Ende der dritten Woche beibehalten und dann langsam damit beginnen, die großen Sonnensegel zu entfernen, damit die Blüten ordentlich Licht abkriegen 💡✨. Zusätzlich probiere ich etwas Neues aus, um den „Emerson-Effekt“ zu aktivieren 🌄: Ich lasse das tiefrote Licht 5 Minuten vor Sonnenuntergang und 5 Minuten nach dem Ende des Tages weiterlaufen. Das sollte der Photosynthese einen letzten Schub geben und das Wachstum der Pflanzen maximal fördern! Bis jetzt sieht alles fantastisch aus! Die Black Cherry ist in verschiedenen Versuchsreihen gut angewachsen, und am Ende des Grows werde ich einen ausführlichen Bericht darüber abgeben, wie sich die beiden Sorten am besten pflegen lassen 🌺👌.

IVE MADE MORE VIDEOS BUT THEY JUST DON'T SEEM TO UPLOAD AND IT'S FAR TO SLOW TO DO IT FROM HOME 6/27 Made last week a five day week to get back on track. It's still overcast and rainy. It's not raining a lot bit it's consistent. Despite the weather the plants are doing phenomenal. I'll update later. It's 1pm. It's been raining consistently since 11. Just a sprinkle but it's steady. I'm going to begun uploading the weeks weather on my diary. I may start a new diary for the plants I light depped as they are flowering pretty good. Rain stopped and it's just overcast for now. I looked at some videos and did a comparison of videos one week ago and videos today and HOLY SHIT! WHAT A DIFFERENCE. Especially the light depped 10th planet. Well everything but that was the most significant difference. I'm astonished at the health and growth despite the crummy weather. Continued to rain. Just got harder. Plants are taking it but it's flooding underneath the pallets a little but it will be fine. The light dep however has me concerned. The 10th planet is looking spectacular. The bigger purple punch I'd looking good too. The smaller one though looks to have a pollen sack coming off one of the branches. Considering its not on the otherside I assume it's not just a swollen calyx. I don't mind chucking it especially if that means I don't hurt my other girls so I want to make sure. I sent videos to a few other growers and I'll add a question on here. Those three plants have been isolated from the rest for a few days due to rain. I have the suspect isolated alone until I can confirm. It sucks cause the light Depp was going good and the6ve all got little flowers. 6/28 Well that fucking sucks. ALL THREE plants I tried to light depp hermed on me. I could see male flowers. Luckily I had been keeping a really good eye on them and it was preflowers mostly. At least I caught it. One or two stamines on each plant. Would've been really easy to miss. Only one had STARTED to elongate into a stem so I think I caught it early enough. Plus since all this rain they've been kept in a different location then my big girls. Glad I did that now. Boy the roots looked good on those plants. I just grabbed the stalk and lifted and it came right out of the pot. I held it there admiring it for a minute. This sucks. At least the real plants are doing good. As far as I know. No male preflowers that's for sure. I've got some feedback from other growers and the videos are a little blurry but I had found a light leak and I'm certain these plants hermed. I know I could've tried to save them but I didn't want to risk it. I compared what I was seeing with Google photos and other websites. Aside from the larger ball with its stem, there were also several little bumps besides developed calyxes that were weaving into little buds. Trust me that I wouldn't cut down my plants if I wasn't 110% sure. I might've been able to "save them" but to me it's just not worth the risk. 6/29 I was second guessing myself pretty hard last night due to some responses I got on my light dep and messages I got from other growets. Made my anxiety horrible but I looked on several video's I'd taken again and I know what I saw. I felt better after that. This was after I researched and waited THREE days until I saw the ball on the stem and the groupings of small nubs under a fresh yellow flower. These plants were flowering good and it sucks to lose them. One MAY have been ok but one was a runt and had all the characteristics of a true hermaphrodite. They were only in 3's and I couldn't risk my harvest for an experiment. Still sucks. Oh well. Sun is starting to come out. Plants seem to be doing fantastic. I have one spot on a leaf that looks like a pillar munched on a leaf so I'll probably get the bt out soon as I have a dry day that I can apply it. I'll have to check the weather. I need to start a nute regiment but the plants aren't telling me they need anything yet. 6/30 I fucked up dates or dodnt do it yesterday or it didnt save right so I'm leaving this blank today is the 1st. 7/1 I have still only watered s couple times and I haven't had to feed. This week I'm going to start nutes. I had some external ersonal situations that have kept me from my plants. I'm hoping to get back on track. I noticed some pillar damage so I'll need to dig out the BT. This morning I saw this giant ground hog by my cage. Hated too but had to get rid of him. Of course some of the blowback landed on the leaves of one of my plants. I tried to clean it as best I could. Better than that fat bastard eating everything in one night. I broke a branch either falling around it or bulling through when I was pissed or I LST it the wrong way and the wind broke it against the tomato cage. Nice big branch too on top. I tried to fix it with duct tape but we'll see. The plants need me to spend sometime with them. I need to clean them up. Apply bt and give them their first feeding. I'll update as I go. They don't seem nutrient deficient by any means but I don't think it would hurt to start the nutes. 7/2 Bags were lighter today and if it wasn't going to rain tonight and tomorrow I'd he watering. Plants look great so soil isn't depleted yet I guess. They're growing rather rapidly. The branch I broke didnt make it. Had an idea it wouldn't but I had to try. I waited on the BT on account of the rain. I may go back over and change my mind and water with silica or a mild nute solution or maybe apply the BT. Depends what time I get back. I have some work I need to do over there. There's a few that I need to clean up the bottoms on. Pest damage is minor and limited to one or two plants and a leaf or two only. 7/3 More rain. It was supposed to rain this morning too but it didn't. We got .33in yesterday and through last night so I thought that was ok. Looking back on my previous diaries I'm doing things significantly different than before. I had used a lot more nutes earlier on. This morning I mixed two gallons of 2tsp of big bloom and fed it to the 9 plants in smart pots leaving the container plants as they have much more water in them. Looking back at other diaries I previously had, WPM and septoria by this time not to mention a shit ton of other pests I was fighting by this time. Since I poisoned where the cagexwas multiple times and sprayed the cage before it was moved I luckily don't have that problem yet knock on wood. I'm planning to apply BT tonight to deal with the moth larvae if there are any. I'm looking at plants around this area and im seeimg SOME septoria and pm on raspberry bushes and burdock so it is around. I made sure my cage is not by any other vegetation this year and is sitting on asphalt with the bags on raised pallets. Good thing I did or I guarantee they'd be flooded by now. I've been seeing multiple complaints from maine growers online (AND THEY HAVE HEALTHY PLANTS!) saying this is the worst year ever. Maybe they need a dose of fusarium oxysporum to keep them humble. This is maine. If you don't like the weather just wait five minutes. Meanwhile I'll be doing my sun dance hoping for sun. "Hard to grow cannabis with no sunlight" said another grower on my forum.

Day 59, 17th of November 2021: Well, not much to mention but the most important: Pistils are coming so the flowers are on the way!!!!! Set up is the same nothing has changed: ViparSpectra XS2000 is on full power which is 240W so 100% capacity. Ventilation: 12 hours period when the lamp is on it switches 1 hour on and after one hour off mostly to keep the temperature and of course because of fresh air flow. When the lamp is off it switches on in every 2 hours for 15 min at the same time with the extraction fan. The extraction fan is on 12 hours when the lamp is, and then it switches on in every 2 hours for 30 min. That's all a bit more water for them from today also. Humidity quite the same approx 50%, temperature as well approx 26-27 Celsius. Fertilization remains the same since last week, the BioBizz family raised up to be 2ml/L each so it is like 2ml/L of BioGrow, 2ml/L of BioBloom, 2ml/L of TopMax... see the ratio above. The schedule of the fertilization has not changed happnes 2x a week except epsom salt just 1x a week.

Muchas gracias Pablo plagron 🙌 Esquejes día 1 Oxígeno concentrado Generador de micro nano burbujas Tanque del agua 300% de oxígeno

58 days of flowering: I started cutting the most mature apicals 42/57. I will wait for the remaining 7-14 days. first 42: 370.5g

Finally starting flowering :D

Incredibile ma vero...ho trovato alcuni ragnetti rossi in una zona del palco. Dopo aver rimosso la parte "infetta",ho dato una passata di piretro(essendo solo alla seconda di fioritura,non dovrebbe creare peoblemi ma,per ogni evenienza,mi sono procurato dell'olio di neem) e per ora,sembra che i ragnetti rossi siano definitivamente deceduti,speriamo tutti. Cmq il mio bellissimo veleno cresce e ci sono un sacco di cime che si stanno riempiendo di peletti bianchi

Doing great as you can see, flipped schedule to 12/12 hope it goes into flowering in a couple of days. Plant is loving the rqs organic pellets in the substrate

35 dias de actividad en el indoor, foto periodo de 20 horas de luz y 4 de oscuridad

the day after being topped the ledies look good😎 Irene (the sativa dominant) reacted very well to LST😄 . the ladies are growing really fast👌 i've lowered a bit the nutrient

She is alot different than last week shi is so bushy and she is indica for sure Day 49 I find her even more bigger I upload video too she is way wider than door 🚪 and she grow in 6L I'm shock she is too big

🌱: Topping on day 13 💧 : 5l 💡 : Dli: 35 mol/m²/d 🤔: Switch to Spider Farmer equipment on day 13