Momma is just about ready for her 36-hr pre-harvest dark period. Going to give her 1, maybe 2, more short light cycles (~10 hrs). Trichomes are mostly cloudy with some amber. Pistils have darkened and curled in. Buds are plump and firm. It’s time! 😎

💜PINK RUNTZ 💜

Amazing amnesia smell, very powerful and strong, just like her sister, looks like this genetic by gea is super stable, both phenos 1 and 2 have the exact same pungent strong amnesia smell and the resin production is 🔝, very sticky flower and super dense and hard as well guys let's see what happens at the end! 💛💚❤️👨‍🌾

August 15th water August 16th nothing August 17th the weather is better so im not sure if i want to leave this plant on the balcony or if i will put her on the roof again, but at the moment in the 15 liter pot shes getting bigger and bigger, so maybe i will just finish this one on the balcony and accommodate the roof better August 18th first 'hairs' appeared 😁 we also watered August 19th nothing August 20th nothing: but it seems that the plant likes the guanokalong nutrients, no signs of deficiency or overfeeding! August 21th: water

No more base nutrients. Just feeding with extra Boost and a small bit of PK. I will reduce this further nearer to chop. I think this is the last week now. 2 girls have maybe another 2 weeks to go. But the other 26 are almost done. 2 different phenos. 1 is shorter and compact. Finishing quicker. The other wants to stretch and finish a week or so after.. Both smell like grapes, black current, lemon and a touch of deisal. Very beautiful! Reduced the light today. 11hrs light. 13hrs dark Much love Peace 💚

Looking for optimal led height. RQS' Northern Lights auto and Diesel auto look very sophisticated and detailed. They are standing in the middle. Rest of the plants are auto lemon Hazes and they have may varieties. Two are growing very fast and two are stunted. At least one more week of indoor growing before going outside in a bigger pot.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Curling is still a problem still :( A rainy week so high humidity all around... looking tasty though! Buds are getting bigger and sticker!

Day 45 17/08/24 Saturday Another watering today using de-chlorinated tap water pH 6 only. Lemon skittles and XXL ammo are tall, bud nice and smelly plants! The Kabul is more of a dense compact build, I'm excited to see what they grow into 😍 Picture and video update ✌️ 💚 Day 48 20/08/24 Tuesday Feed today using de-chlorinated tap water pH 6. With Plagron PK13-14 and power buds. Noticing the stretch stop, and now packing out there bud sites 💪💚 Vid + pic update ✌️💚

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Esta semana he tenido un poco de problemas con la planta debido al cambio de clima para la floración de mi planta cambio a un lugar selo exterior el clima está algo frío y con poco sol pero igual se ha desarrollado muy bien esta semana 🙏🙏🙏🙏

After 21 days in turbo cloner I transplanted 4 Sweet seeds Green Poison into 1 gallon plastic pots filled with Cana coco. Few days later I transplanted another 4 clones into DWC! Stay tuned! Coco feed at 700ppm Dwc feed at 480ppm

Ende der sechsten Woche: Die Pflanzen wurden heute ein letztes mal entlaubt. Bis zur Ernte bleibts jetzt so - außer irgendwelche Blätter geben von alleine den Geist auf. Der Geruch ist inzwischen wirklich heftig! Der Aktivkohlefilter leistet aber gute Arbeit, daher gehts klar. Nach wie vor werden die Buds dicker und dicker. Sie sehen etwas weit für ihr Alter aus, aber ich schiebs mal aufs Adlite… Die Blüten sitzen VOLL mit Trichomen - bin wirklich gespannt, wie das Endprodukt wird.

The weather is suddenly getting pretty cold outside so I have to take the plant inside so she can sleep in a warm place. The next few days should get warm again Found a bug on my plant on Sept. 24th, think they might be thrips After removing the bug I sprinkled the plant with a rape oil and water solution

Diese woche war ziemlich stillstand. Denke auch das sich nichtmehr viel tun wird. Tippe das sie in ca 3 wochn fertig sind. Werdn noch ein bisschen an gewicht zulegen denk ich. Aber viel abwerfen werden sie nicht. Sind aber ideal für extrakte. Sind ab tag 14 komplett mit resin überzogen umd wird von tag zu tag mehr.

Week 19/02 - 25/02

Tag 92 , Noch 2-3 wenige Wochen und die Tage der Ladys sollten gezählt sein . 😍🙌👏

This wedding cake is looking as healthy as her sister however she's in a bigger pot 25 liters. Let's see if she does as good as her sister,she's enjoying life right I don't think I'll have any type of issue,she's being fed 100% organically,with lactobacillus liquid made by me,liquid bat guano,and she has in her soil florians living organics and more bat guano and seaweed,mycorrizae,humic and fulvic acids,beneficial bacteria and fungus. She's gonna be a wonderful plant at the end I'm sure. Stay tuned guys! 💚🌱✌️