Another beautiful

Here we are in week six of flower and not a lot has happen this week other than the usual watering and letting her do her thing. Only trouble this week was updating GoPro had to try a few times. Other than that we got some rain in my home town and collected about 60 gallons that I'm going to use to finish the rest of the grow. Also, got my new AC Infinity CLOUDLINE T4, Quiet Inline Duct Fan System with Temperature and Humidity Controller, 4-Inch. I was really hoping this two plants would finish before the year ended, however, it looks like they got others plans. See you guys next week.

Last day of week 5 I been high asf I almost forgot about this lately lol but you see the results

Wunderbar👍

Hey folks. Into week 5. Nothing much happening here, some lst, hst, topping and some more defoil. Broke a branch while applying lst and taped her back up. They have been consuming more water now so I increased it from 4L every 2-3 days to 6L for the larger gals.

I’m not too happy with the rate of growth but I knew I would be as I didn’t put dynomyco in (purposely) I’ve been feeding every 2 weeks (top feed) with Phyco myco (started last feed) and am seeing some beneficial changes! But will see in time just how much! I was 1” ahead last time and 10” ahead the time before that! So will be running the organic fertiliser next time with dynomyco and just feeding water and seeing the outcome of that! Autos are my testing ground now! As I know exactly what to do with photos! 😉

Hello everyone, I've got 517 gram weet buds, trimed and clean buds, Dry was 133 gr. One normal size joint for two person is enough. You are so happy and high. Awesome strain <3 R.I.P Franco <3

I'm satisfied from tbe overall yield, 65 grams dry is good, but I would have expected more. The smell and taste are awesome and it's very strong, it gives you good appetite and the high last long. Buds are a little bit airy but this must be that the light source is not enough. That's all lads, have been a pleasure updating this diary, and now I'll focus on the other one running that is coming up really nice. It's not a very cultivated phenotype on the site, so come and check it out! Stay tuned and stay high nice people!!

Day 17 well I think I’ll try and price LH & MD sometimes later this week or next. Happy to see the Dr has finally shown up and is starting to stretch. Hopefully a couple more dominate limbs will show, and will trim her up too. As of Saturday Fozzy Bear (we wanted it Fozzie but official paper work says otherwise) joined the family and his new big brother Buddy. He has already grown a few inches since we got him but god is he ever cute, and god bless Buddy for taking his playful shit since

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Amo este fenotipo tanto como el otro esta genetica es magistral,cogollos compactos,pegajosos,super duros un aroma muy rico y agradable,no se puede pedir mas,este es el último riego que realizo con nutrientes a partir de ahora comienzo a lavar las raices por tanto solo regaré con agua con su ph bien ajustado a 6,5 para que ka planta se vaya alimentando de todo de lo que quede y eliminar cualquier resto que pueda quedar que empeora la calidad a la hora de fumar,exquisitez de 00 seed bank!

Week 6 Day 38 of flower 08/14/2020 ~Average~ Temp: 76-84f RH: 42% Ph: 5.9-6.2 Notes: Everything is right on schedule. These ladies are swelling by the day, trichromes production is still at large. The trichromes are starting to be noticeable from the eye. I checked with my digi scope and the trichromes are mostly clear with milky, little to no amber. I will keep my eye on those these next couple of weeks. Happy cultivating✌️🏽

Alles gut👍

Hello my friends 😇 I'm very sorry for missing updates, I'm very busy man in last weeks so I have very limited time for myself thats why I get silent for a while 😑 Good think is all of my girls doing great or even better 😅 I didn't add any new pictures for few days, different between that days is amazing, what do you think ?? Have a good night 😴 You Lovely Girls Growers 😇 💪 🙏

War ein run der seine Zeit gebraucht hat und zum Glück bekommen hat. Ist Mitte der Blüte ins den Wintergarten gezogen, das hat geholfen.

Hello and welcome to week 8. Enigma is on day 53 now and has partially recovered from the burn. Hopefully she will steady up as she goes. I thinn 3 or 4 weeks is what we can expect. Does she have to be harvested before the competition ends? Day 55: Not much change. Day 56: End of week. Roll on week 9!