Dropped light hours down to 16 . Topped off nutrient solution with 5.0 ph evened out .removed none chosen phenos to be flowered in separate room no longer tracking those we will only follow the chosen mothers clones i cut clones today as well had the lady friend clean floor with bleach and dawn put clones in ez cloner 32 systems

Week 8 Watering in at 700ml getting lots of color!

Great growth this week. She is looking fine, really strong pheno from great genetics. Starting to give off a small scent. I’m feeding every 3 days at the minute.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Empecé a hacer estrategia de riego de precisión, acumule ec en el sustrato hasta llegar a 3000ms de salida en la escorrentia, voy a tratar de mantenerlo ahí unos días a ver como reaccionan las plantas, por ahora parece que hasta están pidiendo más comida aun. Hice defoliacion y acomode las ramas en la red y quedo casi toda la superficie cubierta. La luz en el medio llega a 1100PPFD y en los costados más alejados unos 700PPFD

Esa familia, buenas de nuevo, siento la tardanza, asuntos propios ya me entendéis... pero ya actualizo y es que esta farm cheese de genofarm esta ya apunto de caramelo. Se espera el corte muy pronto y la verdad que promete , se ve increíble y huele que flipais. Ph controlado temperatura por debajo de 27 grados y humedad por debajo de 40%. Estoy muy contento porque el genotipo cheese es de mis preferidos y está en concreto tiene ese olor tan peculiar. Un saludo y nos vemos fumetillas.

I didn’t take pictures before defoliation but these pics are from after the fact So you can only imagine. So many bud sites she should be stacked!!! The game plan is to move her into a 5 gallon once I get a chance this week. Glacial, 2-8-4 and some kelp meal will be used.

Day64/Flower16 03.03 Optimized my setup. I can handle humidity better now. Just in time. Puuh. I also changed back to hesi nutrients. Plants are really thirsty now. Watering every 2 days. Actually using about 12 liters. About 3 liters per plant every second day. Plants are really looking nice. Super excited. Plants are super easy to train, amazing flexibility. You can almost do knots with the branches. :) Growers life is an adventurous life...yeah... Day67/FL19 06.03 Everything looks fine. Planning to water them everyday as long as i'm not busy. Trying to figure out the exact amount of hesi nutrients..

never thought this clone would survive taken day 15 of flowering from my previously monster cropped tropicana cookies

Going into darkness within next few days for 48hours.. She smells amazing. Buds appear very dense. Overall very pleased. A few things I should have done differently but none the less a good grow. Today is day 61 from light switch.

Se realiza trasplante a macetas definitivas de 10 Litros Geotextiles, se aplica super soil con 3 productos orgánicos de la marca Yeskaya nacional (chile) mezclando un total de 90 Litros, se riega con 200 ml por planta con Great White y Orca. Se realiza Riego Foliar con Knactive producto antiestrés nacional (chile). Todo con PH 6.0 yeskaya.cl www.instagram.com/knactive_cl

the two rainbows are on flowering Time and we also changed the ciclus for the san Fernando valley also from B.S.F. Seeds, we are expecting the TnB naturals #co2enhancerbottle

I hope you will enjoy my work, the peaks are to dry, share a few days for comments or advice, or questions please write to me.... !! peace & love

day 15 - 27/11/2025. all looking good, healthy foliage all around. beginning of week 3. did a early def on the tiniest of them all, due to her leaves shadowing herself. so hopefully it will help her to get more light into the canopy

Buds were delicious and very heavy high! Definitely night time gear. Beautiful aroma and tastes of fruit loops. Pretty sure I topped this plant. Won't be doing that again as I've had better success with light lst. Very happy with the quality of cannabis produced from this plant!

it's amazing, this growth looks much better than my first attempt, my decision was to put a smaller pot and change the light - the girl really liked it, FastBuds goriilla cookies with a small space manage perfectly:).

Journal de culture - Semaine 3 de croissance La plante a bien repris sa vigueur cette semaine, développant une belle masse racinaire, ce qui est très encourageant. Elle continue de se développer et je m’efforce de la maintenir la plus basse possible en utilisant des techniques de Low Stress Training. J’ai essayé le pinching, qui consiste à pincer légèrement les extrémités des branches pour les courber. Cela permet de rediriger les hormones de croissance vers les parties inférieures, ce qui maximise le développement des pousses. J’ai également effectué une légère défoliation en enlevant quelques grandes feuilles superflues. Pour le reste, je surveille principalement le pH. J’essaie de le maintenir autour de 5.8, mais avec les tampons présents dans les produits Terra Aquatica, il remonte régulièrement à 6.2. Je me demande si ce pH légèrement élevé pourrait être à l’origine d’un problème de coloration des feuilles, car la plante semble présenter une légère carence malgré la présence de tous les nutriments nécessaires. Je fais attention à ne pas ajouter trop d’acide afin de ne pas surcharger la solution nutritive. Globalement, la plante est en bonne santé et continue de bien évoluer. Je pense passer en floraison d’ici une ou deux semaines maximum. Étant donné qu’il s’agit d’une variété Jack Herer, qui a tendance à beaucoup s’étirer en floraison, je préfère la maintenir encore un peu en croissance pour travailler tranquillement avec les techniques de Low Stress Training et la défoliation. On verra son évolution la semaine prochaine ! 🇺🇸 Grow Diary - Week 3 of Veg The plant has regained its vigor this week, developing a strong root mass, which is great. It keeps growing, and I’m trying to keep it as low as possible using Low Stress Training techniques. I’ve been trying pinching, which involves lightly pinching the tips of the branches to bend them. This redirects the growth hormones to the lower parts, maximizing the development of new shoots. I’ve also done a bit of light defoliation by removing some large, unnecessary leaves. Other than that, I’m mainly monitoring the pH. I’m trying to keep it as close to 5.8 as possible, but the buffers in the Terra Aquatica products keep raising it to 6.2. I’m wondering if this slightly higher pH might be causing some discoloration on the leaves—it feels like a deficiency, even though all the necessary nutrients are present. I’m cautious about adding too much acid to avoid overloading the nutrient solution. Overall, the plant is healthy and continues to grow well. I’m thinking about switching to flowering in a week or two at most. Since this is a Jack Herer strain, which tends to stretch a lot during flowering, I want to keep it in veg a bit longer while working with Low Stress Training and defoliation. We’ll see how it evolves next week! 🤘😎🤘

Ok so this week I did a defoliation and a tigh down too the netting , I really was not planning on doing such a big defoliation but the leafs became thick and dense and there was almost no light penitration down too the lower branches of the plant , so she is opened right up now and the humidity and temperature has dropped loads and the air is moving alot better around the leaves and branches , I will not take anything more off her now , I have also added Cal mag too the nutrients for this week only , she is well into pre flower now but the stretch has not yet started so I figured it's no or never too defoliate and tigh them down , I am very happy with them so far and being new too all this it's all very exciting , Thanks for looking :)