Lots of pics not much to report. Flushing and starting to yellowing nicely. Trichomes are in check so I plugged light out and left these two girls in darkness for 36-48h. These yielded clearly most in this round. Nice job Barney's with this one 👌 Can't wait to smoke this one out once cured couple weeks 👊😍

Harvest week coming up. Plant looks really good and Colas have gotten really heavy

Eccoci qui, Settimana all'insegna del Topping e dell'LST per far si che la piccola cresca con cime di serie a! Verrà eseguito scrog a breve non appena si allungheranno le cime. Topping eseguito il 16/01/2024, dopo un giorno ha reagito molto bene, sarà interessante lo sviluppo!!! Grazie a tutti per il supporto ❤️🔥🌲

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Height on 1 of these now 🚀🚀 🔥🔥🔥 I topped the big one yesterday to try get an even canopy before flipping to flower 2 weeks and we flip em!

What you guys think of my 1st attempt off growing I've learnt a hell off a lot from this grow it wasn't as easy as it looks I give my plants a lot off attention some times I think a little bit to much but this was all a learning curve very excited to havest her and continue with my other projects... The next one will be a lot more detailed and hoping for a lot better results.. Put what I've learnt to the test... Thanks guys for all your help

great, everything is going really smoothly, @Zamnesia Runtz auto, copes perfectly with leaf spot and LST, started to bloom, so I think it will soon stop growing upwards, and I won't have to hurt it anymore :) @Plagron This is the first time I've used nutritional fertilizers, but I already understand that it definitely won't be the last :) good luck to everyone :).

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 64 since time change to 12/12 h. Hey guys :-) The last days are numbered for the mini lady :-). The coming days it will be in the darkroom for 48 hours before it is carefully trimmed by hand :-). The week was two times poured with 1 l each. The tent was cleaned and everything checked. Have fun with the update and stay healthy 🙏🏻 You can buy this Strain at : https://www.zamnesia.com/de/5165-zamnesia-seeds-kalini-asia-feminisiert.html Type: Kalini Asia ☝️🏼 Genetics: Black Domina x Purple Kush 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

Not a great selection. Low yield. Hopefully it will taste and smoke great as it looks.

So those girls were transfered from main room, theirs veg story you'll find in Checkpoint diary by Sweet Seeds. In reality they veged for 5 weeks, beans didn't want to sprout, so planted two more. One from first two- didn't make it... Everything with them postponed a bit and i decided to try out other training method and new version/interpretation of FIMing. They got 30 hours of darkness, looked sad a bit, but just 3 hours under new sun - they are happy as f@ck again ;))) Will feed them later today with full range of BioBizz. Will update charts. Day 6. Girls loved food, no big stretch yet ... Happy Growing !!!

Time for drying. Btw drying them like this was a bad idea buds are pressed from one side may be conceded to move them, well I still prefer hanging drying 🤪

Super sticky frosty buds😬🔥👊🏻

All three plants are happy and showing a nice layer of trichomes. Still in 2litre pots.

English: I decided to switch to 12/12 after week 6. The risk of the plants becoming too big for the tent was too great for me. Since switching to 12/12, I have also reduced the amount of water and am trying to push the EC in the substrate further in the 3.5-5 direction. I am still giving the full dose of Grow A & B and will continue to do so until week 3 or 4, depending on how they develop. I will also increase the PPFD from 500 to 900 within the first 3 weeks of flowering. Everything seems to be working well so far, the ladies are growing and also got their next defoliation at the end of the 1st week of flowering, as it was getting very dense. New updates coming next week :) Deutsch: Ich habe mich doch dazu entschieden nach Woche 6 auf 12/12 zu switchen. Das Risiko, dass die Pflanzen zu groß für das Zelt werden, war mir zu groß. Seit dem Switch auf 12/12 habe ich auch die Wassermenge reduziert und versuche dadurch den EC im Substrat weiter in die 3.5-5 Richtung zu pushen. Ich gebe weiterhin die volle Dosis Grow A & B und werde das auch weiter bis Woche 3 oder 4 tun, je nachdem wie sie sich entwickeln. Außerdem werde ich auch den PPFD Wert innerhalb der ersten 3 Blütewochen von 500 auf 900 hochfahren. Scheint so weit auch alles gut zu funktionieren, die Ladys wachsen und haben am Ende der 1. Blütewoche auch ihre nächste Defoliation bekommen, da es doch langsam sehr dicht wurde. Nächste Woche kommen neue Updates :)

Ventunesimo giorno di vita, tutto procede regolarmente. Non mi lamento della velocità di crescita :) Le foglie più grandi risultano piegate nelle punte perché durante le ore notturne, le foglie più grandi piegandosi toccano terra.

The buds are growing fast the weight are increasing you can see on the pictures ^^ I added the plagron green sensation :)

Still only giving water at ph6.5. I will put them in 4 litre pots soon.